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Protein

Potassium voltage-gated channel subfamily A member 3

Gene

Kcna3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296072. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 3
Alternative name(s):
MK3
Voltage-gated potassium channel subunit Kv1.3
Gene namesi
Name:Kcna3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96660. Kcna3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 187CytoplasmicSequence analysisAdd BLAST187
Transmembranei188 – 206Helical; Name=Segment S1Sequence analysisAdd BLAST19
Topological domaini207 – 247ExtracellularSequence analysisAdd BLAST41
Transmembranei248 – 269Helical; Name=Segment S2Sequence analysisAdd BLAST22
Topological domaini270 – 280CytoplasmicSequence analysisAdd BLAST11
Transmembranei281 – 301Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini302 – 315ExtracellularSequence analysisAdd BLAST14
Transmembranei316 – 334Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST19
Topological domaini335 – 350CytoplasmicSequence analysisAdd BLAST16
Transmembranei351 – 370Helical; Name=Segment S5Sequence analysisAdd BLAST20
Topological domaini371 – 411ExtracellularSequence analysisAdd BLAST41
Transmembranei412 – 434Helical; Name=Segment S6Sequence analysisAdd BLAST23
Topological domaini435 – 528CytoplasmicSequence analysisAdd BLAST94

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4818.
GuidetoPHARMACOLOGYi540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539781 – 528Potassium voltage-gated channel subfamily A member 3Add BLAST528

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi232N-linked (GlcNAc...)Sequence analysis1
Lipidationi270S-palmitoyl cysteineSequence analysis1
Modified residuei473Phosphoserine; by PKASequence analysis1

Post-translational modificationi

N-glycosylation promotes the cell surface expression.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP16390.
PaxDbiP16390.
PeptideAtlasiP16390.
PRIDEiP16390.

PTM databases

iPTMnetiP16390.
PhosphoSitePlusiP16390.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047959.
GenevisibleiP16390. MM.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG4 (By similarity).By similarity

Protein-protein interaction databases

IntActiP16390. 1 interactor.
STRINGi10090.ENSMUSP00000050680.

Chemistry databases

BindingDBiP16390.

Structurei

3D structure databases

ProteinModelPortaliP16390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi397 – 402Selectivity filterBy similarity6
Motifi526 – 528PDZ-bindingBy similarity3

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1545. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP16390.
KOiK04876.
OMAiKRRMRYF.
OrthoDBiEOG091G10NU.
PhylomeDBiP16390.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004050. K_chnl_volt-dep_Kv1.3.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01510. KV13CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P16390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVPGDHLL EPEAAGGGGG DPPQGGCGSG GGGGGCDRYE PLPPALPAAG
60 70 80 90 100
EQDCCGERVV INISGLRFET QLKTLCQFPE TLLGDPKRRM RYFDPLRNEY
110 120 130 140 150
FFDRNRPSFD AILYYYQSGG RIRRPVNVPI DIFSEEIRFY QLGEEAMEKF
160 170 180 190 200
REDEGFLREE ERPLPRRDFQ RQVWLLFEYP ESSGPARGIA IVSVLVILIS
210 220 230 240 250
IVIFCLETLP EFRDEKDYPA SPSQDVFEAA NNSTSGAPSG ASSFSDPFFV
260 270 280 290 300
VETLCIIWFS FELLVRFFAC PSKATFSRNI MNLIDIVAII PYFITLGTEL
310 320 330 340 350
AERQGNGQQA MSLAILRVIR LVRVFRIFKL SRHSKGLQIL GQTLKASMRE
360 370 380 390 400
LGLLIFFLFI GVILFSSAVY FAEADDPSSG FNSIPDAFWW AVVTMTTVGY
410 420 430 440 450
GDMHPVTIGG KIVGSLCAIA GVLTIALPVP VIVSNFNYFY HRETEGEEQA
460 470 480 490 500
QYMHVGSCQH LSSSAEELRK ARSNSTLSKS EYMVIEEGGM NHSAFPQTPF
510 520
KTGNSTATCT TNNNPNSCVN IKKIFTDV
Length:528
Mass (Da):58,564
Last modified:November 25, 2008 - v3
Checksum:i12AC94FB13A523E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti369V → A in AAA39716 (PubMed:2305265).Curated1
Sequence conflicti492H → Q in AAA39716 (PubMed:2305265).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30441 Genomic DNA. Translation: AAA39716.1.
CH466607 Genomic DNA. Translation: EDL01888.1.
BC132349 mRNA. Translation: AAI32350.1.
BC137667 mRNA. Translation: AAI37668.1.
CCDSiCCDS17730.1.
PIRiC40090. I84205.
S09044.
RefSeqiNP_032444.2. NM_008418.2.
UniGeneiMm.30640.

Genome annotation databases

EnsembliENSMUST00000052718; ENSMUSP00000050680; ENSMUSG00000047959.
GeneIDi16491.
KEGGimmu:16491.
UCSCiuc008qwp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30441 Genomic DNA. Translation: AAA39716.1.
CH466607 Genomic DNA. Translation: EDL01888.1.
BC132349 mRNA. Translation: AAI32350.1.
BC137667 mRNA. Translation: AAI37668.1.
CCDSiCCDS17730.1.
PIRiC40090. I84205.
S09044.
RefSeqiNP_032444.2. NM_008418.2.
UniGeneiMm.30640.

3D structure databases

ProteinModelPortaliP16390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16390. 1 interactor.
STRINGi10090.ENSMUSP00000050680.

Chemistry databases

BindingDBiP16390.
ChEMBLiCHEMBL4818.
GuidetoPHARMACOLOGYi540.

PTM databases

iPTMnetiP16390.
PhosphoSitePlusiP16390.

Proteomic databases

MaxQBiP16390.
PaxDbiP16390.
PeptideAtlasiP16390.
PRIDEiP16390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052718; ENSMUSP00000050680; ENSMUSG00000047959.
GeneIDi16491.
KEGGimmu:16491.
UCSCiuc008qwp.2. mouse.

Organism-specific databases

CTDi3738.
MGIiMGI:96660. Kcna3.

Phylogenomic databases

eggNOGiKOG1545. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP16390.
KOiK04876.
OMAiKRRMRYF.
OrthoDBiEOG091G10NU.
PhylomeDBiP16390.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiR-MMU-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiP16390.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047959.
GenevisibleiP16390. MM.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004050. K_chnl_volt-dep_Kv1.3.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01510. KV13CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNA3_MOUSE
AccessioniPrimary (citable) accession number: P16390
Secondary accession number(s): A3KMM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 25, 2008
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.