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P16390

- KCNA3_MOUSE

UniProt

P16390 - KCNA3_MOUSE

Protein

Potassium voltage-gated channel subfamily A member 3

Gene

Kcna3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome
    2. outward rectifier potassium channel activity Source: Ensembl

    GO - Biological processi

    1. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199077. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 3
    Alternative name(s):
    MK3
    Voltage-gated potassium channel subunit Kv1.3
    Gene namesi
    Name:Kcna3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:96660. Kcna3.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane raft Source: Ensembl
    2. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 528528Potassium voltage-gated channel subfamily A member 3PRO_0000053978Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
    Lipidationi270 – 2701S-palmitoyl cysteineSequence Analysis
    Modified residuei473 – 4731Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    N-glycosylation promotes the cell surface expression.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP16390.
    PRIDEiP16390.

    PTM databases

    PhosphoSiteiP16390.

    Expressioni

    Gene expression databases

    BgeeiP16390.
    GenevestigatoriP16390.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG4 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP16390. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP16390.
    SMRiP16390. Positions 57-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 187187CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini207 – 24741ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini270 – 28011CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini302 – 31514ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini335 – 35016CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini371 – 41141ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini435 – 52894CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei188 – 20619Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei248 – 26922Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei281 – 30121Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei316 – 33419Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei351 – 37020Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei412 – 43423Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi397 – 4026Selectivity filterBy similarity
    Motifi526 – 5283PDZ-bindingBy similarity

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115256.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiA3KMM2.
    KOiK04876.
    OMAiKRRMRYF.
    OrthoDBiEOG7M0NRD.
    PhylomeDBiP16390.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004050. K_chnl_volt-dep_Kv1.3.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01510. KV13CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P16390-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVVPGDHLL EPEAAGGGGG DPPQGGCGSG GGGGGCDRYE PLPPALPAAG    50
    EQDCCGERVV INISGLRFET QLKTLCQFPE TLLGDPKRRM RYFDPLRNEY 100
    FFDRNRPSFD AILYYYQSGG RIRRPVNVPI DIFSEEIRFY QLGEEAMEKF 150
    REDEGFLREE ERPLPRRDFQ RQVWLLFEYP ESSGPARGIA IVSVLVILIS 200
    IVIFCLETLP EFRDEKDYPA SPSQDVFEAA NNSTSGAPSG ASSFSDPFFV 250
    VETLCIIWFS FELLVRFFAC PSKATFSRNI MNLIDIVAII PYFITLGTEL 300
    AERQGNGQQA MSLAILRVIR LVRVFRIFKL SRHSKGLQIL GQTLKASMRE 350
    LGLLIFFLFI GVILFSSAVY FAEADDPSSG FNSIPDAFWW AVVTMTTVGY 400
    GDMHPVTIGG KIVGSLCAIA GVLTIALPVP VIVSNFNYFY HRETEGEEQA 450
    QYMHVGSCQH LSSSAEELRK ARSNSTLSKS EYMVIEEGGM NHSAFPQTPF 500
    KTGNSTATCT TNNNPNSCVN IKKIFTDV 528
    Length:528
    Mass (Da):58,564
    Last modified:November 25, 2008 - v3
    Checksum:i12AC94FB13A523E7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti369 – 3691V → A in AAA39716. (PubMed:2305265)Curated
    Sequence conflicti492 – 4921H → Q in AAA39716. (PubMed:2305265)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30441 Genomic DNA. Translation: AAA39716.1.
    CH466607 Genomic DNA. Translation: EDL01888.1.
    BC132349 mRNA. Translation: AAI32350.1.
    BC137667 mRNA. Translation: AAI37668.1.
    CCDSiCCDS17730.1.
    PIRiC40090. I84205.
    S09044.
    RefSeqiNP_032444.2. NM_008418.2.
    UniGeneiMm.30640.

    Genome annotation databases

    EnsembliENSMUST00000052718; ENSMUSP00000050680; ENSMUSG00000047959.
    GeneIDi16491.
    KEGGimmu:16491.
    UCSCiuc008qwp.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30441 Genomic DNA. Translation: AAA39716.1 .
    CH466607 Genomic DNA. Translation: EDL01888.1 .
    BC132349 mRNA. Translation: AAI32350.1 .
    BC137667 mRNA. Translation: AAI37668.1 .
    CCDSi CCDS17730.1.
    PIRi C40090. I84205.
    S09044.
    RefSeqi NP_032444.2. NM_008418.2.
    UniGenei Mm.30640.

    3D structure databases

    ProteinModelPortali P16390.
    SMRi P16390. Positions 57-444.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P16390. 1 interaction.

    Chemistry

    BindingDBi P16390.
    ChEMBLi CHEMBL4818.
    GuidetoPHARMACOLOGYi 540.

    PTM databases

    PhosphoSitei P16390.

    Proteomic databases

    PaxDbi P16390.
    PRIDEi P16390.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052718 ; ENSMUSP00000050680 ; ENSMUSG00000047959 .
    GeneIDi 16491.
    KEGGi mmu:16491.
    UCSCi uc008qwp.2. mouse.

    Organism-specific databases

    CTDi 3738.
    MGIi MGI:96660. Kcna3.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115256.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi A3KMM2.
    KOi K04876.
    OMAi KRRMRYF.
    OrthoDBi EOG7M0NRD.
    PhylomeDBi P16390.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199077. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 289791.
    PROi P16390.
    SOURCEi Search...

    Gene expression databases

    Bgeei P16390.
    Genevestigatori P16390.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004050. K_chnl_volt-dep_Kv1.3.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01510. KV13CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A family of three mouse potassium channel genes with intronless coding regions."
      Chandy K.G., Williams C.B., Spencer R.H., Aguilar B.A., Ghanshani S., Tempel B.L., Gutman G.A.
      Science 247:973-975(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNA3_MOUSE
    AccessioniPrimary (citable) accession number: P16390
    Secondary accession number(s): A3KMM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3