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P16389 (KCNA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily A member 2
Alternative name(s):
NGK1
Voltage-gated K(+) channel HuKIV
Voltage-gated potassium channel HBK5
Voltage-gated potassium channel subunit Kv1.2
Gene names
Name:KCNA2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Domain

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Post-translational modification

Phosphorylated on tyrosine residues by activated PTK2B/PYK2; this regulates ion channel activity By similarity.

Sequence similarities

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P16389-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P16389-2)

The sequence of this isoform differs from the canonical sequence as follows:
     299-499: VRVFRIFKLS...VNITKMLTDV → ERRPLQSQKS...PAVTTLHRMY
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Potassium voltage-gated channel subfamily A member 2
PRO_0000053972

Regions

Transmembrane164 – 18219Helical; Name=Segment S1; Potential
Transmembrane222 – 24322Helical; Name=Segment S2; Potential
Transmembrane255 – 27521Helical; Name=Segment S3; Potential
Transmembrane293 – 31119Helical; Voltage-sensor; Name=Segment S4; Potential
Transmembrane328 – 34720Helical; Name=Segment S5; Potential
Transmembrane389 – 41123Helical; Name=Segment S6; Potential
Motif374 – 3796Selectivity filter By similarity
Motif497 – 4993PDZ-binding By similarity

Amino acid modifications

Modified residue4291Phosphotyrosine By similarity
Modified residue4491Phosphoserine; by PKA Potential
Lipidation2441S-palmitoyl cysteine Potential
Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation2071N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence299 – 499201VRVFR…MLTDV → ERRPLQSQKSKRGRQHLNTS HDCTLGINLVAGMTVQWTRA SGPDDRQTPAVTTLHRMY in isoform 2.
VSP_043077

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 4B03F1B46A826C39

FASTA49956,717
        10         20         30         40         50         60 
MTVATGDPAD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT LAQFPETLLG 

        70         80         90        100        110        120 
DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR PVNVPLDIFS EEIRFYELGE 

       130        140        150        160        170        180 
EAMEMFREDE GYIKEEERPL PENEFQRQVW LLFEYPESSG PARIIAIVSV MVILISIVSF 

       190        200        210        220        230        240 
CLETLPIFRD ENEDMHGSGV TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR 

       250        260        270        280        290        300 
FFACPSKAGF FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLVR 

       310        320        330        340        350        360 
VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE ADERESQFPS 

       370        380        390        400        410        420 
IPDAFWWAVV SMTTVGYGDM VPTTIGGKIV GSLCAIAGVL TIALPVPVIV SNFNYFYHRE 

       430        440        450        460        470        480 
TEGEEQAQYL QVTSCPKIPS SPDLKKSRSA STISKSDYME IQEGVNNSNE DFREENLKTA 

       490 
NCTLANTNYV NITKMLTDV 

« Hide

Isoform 2 [UniParc].

Checksum: 9917ABBFCCCF7411
Show »

FASTA35640,966

References

« Hide 'large scale' references
[1]"Human potassium channel genes: molecular cloning and functional expression."
Ramaswami M., Gautam M., Kamb A., Rudy B., Tanouye M.A., Mathew M.K.
Mol. Cell. Neurosci. 1:214-223(1990)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Blood.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L02752 mRNA. Translation: AAA36141.1.
AL365361 Genomic DNA. Translation: CAH71982.1.
BC043564 mRNA. Translation: AAH43564.1.
PIRI77466.
RefSeqNP_001191198.1. NM_001204269.1.
NP_004965.1. NM_004974.3.
UniGeneHs.248139.
Hs.657199.
Hs.731191.

3D structure databases

ProteinModelPortalP16389.
SMRP16389. Positions 3-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109940. 16 interactions.
STRING9606.ENSP00000314520.

Chemistry

BindingDBP16389.
ChEMBLCHEMBL2086.
GuidetoPHARMACOLOGY539.

Protein family/group databases

TCDB1.A.1.2.10. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteP16389.

Polymorphism databases

DMDM1345813.

Proteomic databases

PaxDbP16389.
PRIDEP16389.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000316361; ENSP00000314520; ENSG00000177301. [P16389-1]
ENST00000369770; ENSP00000358785; ENSG00000177301. [P16389-2]
ENST00000440270; ENSP00000415257; ENSG00000177301. [P16389-1]
ENST00000485317; ENSP00000433109; ENSG00000177301. [P16389-1]
GeneID3737.
KEGGhsa:3737.
UCSCuc009wfv.2. human. [P16389-2]
uc009wfw.3. human. [P16389-1]

Organism-specific databases

CTD3737.
GeneCardsGC01M111137.
HGNCHGNC:6220. KCNA2.
HPACAB001976.
MIM176262. gene.
neXtProtNX_P16389.
PharmGKBPA206.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1226.
HOGENOMHOG000231015.
HOVERGENHBG052230.
InParanoidP16389.
KOK04875.
OMAMTFHTYS.
OrthoDBEOG7M0NRD.
PhylomeDBP16389.
TreeFamTF313103.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

BgeeP16389.
CleanExHS_KCNA2.
GenevestigatorP16389.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004049. K_chnl_volt-dep_Kv1.2.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERPTHR11537. PTHR11537. 1 hit.
PfamPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR00169. KCHANNEL.
PR01509. KV12CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
ProtoNetSearch...

Other

GeneWikiKCNA2.
GenomeRNAi3737.
NextBio14627.
PROP16389.
SOURCESearch...

Entry information

Entry nameKCNA2_HUMAN
AccessionPrimary (citable) accession number: P16389
Secondary accession number(s): Q86XG6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM