P16389 (KCNA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily A member 2

Alternative name(s):
NGK1
Voltage-gated K(+) channel HuKIV
Voltage-gated potassium channel HBK5
Voltage-gated potassium channel subunit Kv1.2

Gene names

Name:

KCNA2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Subunit structure	Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Post-translational modification	Phosphorylated on tyrosine residues by activated PTK2B/PYK2; this regulates ion channel activity By similarity.
Sequence similarities	Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ionic channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	synaptic transmission Traceable author statement. Source: Reactome
Cellular component	juxtaparanode region of axon Inferred from sequence or structural similarity. Source: BHF-UCL voltage-gated potassium channel complex Traceable author statement. Source: ProtInc
Molecular function	delayed rectifier potassium channel activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Potassium voltage-gated channel subfamily A member 2		PRO_0000053972
Regions
	Transmembrane	164 – 182	19	Helical; Name=Segment S1; Potential
	Transmembrane	222 – 243	22	Helical; Name=Segment S2; Potential
	Transmembrane	255 – 275	21	Helical; Name=Segment S3; Potential
	Transmembrane	293 – 311	19	Helical; Voltage-sensor; Name=Segment S4; Potential
	Transmembrane	328 – 347	20	Helical; Name=Segment S5; Potential
	Transmembrane	389 – 411	23	Helical; Name=Segment S6; Potential
	Motif	374 – 379	6	Selectivity filter By similarity
	Motif	497 – 499	3	PDZ-binding By similarity
Amino acid modifications
	Modified residue	421	1	Phosphothreonine By similarity
	Modified residue	429	1	Phosphotyrosine By similarity
	Modified residue	433	1	Phosphothreonine By similarity
	Modified residue	449	1	Phosphoserine; by PKA Potential
	Lipidation	244	1	S-palmitoyl cysteine Potential
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P16389 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 4B03F1B46A826C39	FASTA	499	56,717
	10 20 30 40 50 60 MTVATGDPAD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT LAQFPETLLG 70 80 90 100 110 120 DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR PVNVPLDIFS EEIRFYELGE 130 140 150 160 170 180 EAMEMFREDE GYIKEEERPL PENEFQRQVW LLFEYPESSG PARIIAIVSV MVILISIVSF 190 200 210 220 230 240 CLETLPIFRD ENEDMHGSGV TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR 250 260 270 280 290 300 FFACPSKAGF FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLVR 310 320 330 340 350 360 VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE ADERESQFPS 370 380 390 400 410 420 IPDAFWWAVV SMTTVGYGDM VPTTIGGKIV GSLCAIAGVL TIALPVPVIV SNFNYFYHRE 430 440 450 460 470 480 TEGEEQAQYL QVTSCPKIPS SPDLKKSRSA STISKSDYME IQEGVNNSNE DFREENLKTA 490 NCTLANTNYV NITKMLTDV

References

[1]	"Human potassium channel genes: molecular cloning and functional expression." Ramaswami M., Gautam M., Kamb A., Rudy B., Tanouye M.A., Mathew M.K. Mol. Cell. Neurosci. 1:214-223(1990) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02752 mRNA. Translation: AAA36141.1.
IPI	IPI00028134.
PIR	I77466.
RefSeq	NP_004965.1. NM_004974.3.
UniGene	Hs.248139. Hs.657199.
3D structure databases
ProteinModelPortal	P16389.
SMR	P16389. Positions 32-421.
ModBase	Search...
Protein-protein interaction databases
STRING	P16389.
Protein family/group databases
TCDB	1.A.1.2.10. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	P16389.
Polymorphism databases
DMDM	1345813.
Proteomic databases
PRIDE	P16389.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316361; ENSP00000314520; ENSG00000177301. ENST00000440270; ENSP00000415257; ENSG00000177301.
GeneID	3737.
KEGG	hsa:3737.
NMPDR	fig|9606.3.peg.1678.
UCSC	uc001dzu.1. human.
Organism-specific databases
CTD	3737.
GeneCards	GC01M111137.
HGNC	HGNC:6220. KCNA2.
HPA	CAB001976.
MIM	176262. gene.
neXtProt	NX_P16389.
PharmGKB	PA206.
GenAtlas	Search...
Phylogenomic databases
eggNOG	maNOG06054.
GeneTree	ENSGT00560000076957.
HOGENOM	HBG445693.
HOVERGEN	HBG052230.
InParanoid	P16389.
OMA	AQYLQVN.
OrthoDB	EOG4DR9CB.
PhylomeDB	P16389.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	P16389.
Bgee	P16389.
CleanEx	HS_KCNA2.
Genevestigator	P16389.
GermOnline	ENSG00000177301. Homo sapiens.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003972. K_chnl_volt-dep_Kv1. IPR004049. K_chnl_volt-dep_Kv1.2. IPR003131. K_chnl_volt-dep_Kv_tetra. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
KO	K04875.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01509. KV12CHANNEL. PR01491. KVCHANNEL. PR01496. SHAKERCHANEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	14627.
SOURCE	Search...

Entry information

Entry name

KCNA2_HUMAN

Accession

Primary (citable) accession number: P16389

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.