P16389 (KCNA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily A member 2

Alternative name(s):
NGK1
Voltage-gated K(+) channel HuKIV
Voltage-gated potassium channel HBK5
Voltage-gated potassium channel subunit Kv1.2

Gene names

Name:

KCNA2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Subunit structure	Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Post-translational modification	Phosphorylated on tyrosine residues by activated PTK2B/PYK2; this regulates ion channel activity By similarity.
Sequence similarities	Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	optic nerve structural organization Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: InterPro synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	juxtaparanode region of axon Inferred from sequence or structural similarity. Source: BHF-UCL voltage-gated potassium channel complex Traceable author statement PubMed 2251283. Source: ProtInc
Molecular_function	delayed rectifier potassium channel activity Traceable author statement PubMed 1715584. Source: ProtInc outward rectifier potassium channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P16389-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P16389-2)

The sequence of this isoform differs from the canonical sequence as follows:
     299-499: VRVFRIFKLS...VNITKMLTDV → ERRPLQSQKS...PAVTTLHRMY

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Potassium voltage-gated channel subfamily A member 2		PRO_0000053972
Regions
	Transmembrane	164 – 182	19	Helical; Name=Segment S1; Potential
	Transmembrane	222 – 243	22	Helical; Name=Segment S2; Potential
	Transmembrane	255 – 275	21	Helical; Name=Segment S3; Potential
	Transmembrane	293 – 311	19	Helical; Voltage-sensor; Name=Segment S4; Potential
	Transmembrane	328 – 347	20	Helical; Name=Segment S5; Potential
	Transmembrane	389 – 411	23	Helical; Name=Segment S6; Potential
	Motif	374 – 379	6	Selectivity filter By similarity
	Motif	497 – 499	3	PDZ-binding By similarity
Amino acid modifications
	Modified residue	421	1	Phosphothreonine By similarity
	Modified residue	429	1	Phosphotyrosine By similarity
	Modified residue	433	1	Phosphothreonine By similarity
	Modified residue	449	1	Phosphoserine; by PKA Potential
	Lipidation	244	1	S-palmitoyl cysteine Potential
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	299 – 499	201	VRVFR…MLTDV → ERRPLQSQKSKRGRQHLNTS HDCTLGINLVAGMTVQWTRA SGPDDRQTPAVTTLHRMY in isoform 2.		VSP_043077

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 4B03F1B46A826C39	FASTA	499	56,717
	10 20 30 40 50 60 MTVATGDPAD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT LAQFPETLLG 70 80 90 100 110 120 DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR PVNVPLDIFS EEIRFYELGE 130 140 150 160 170 180 EAMEMFREDE GYIKEEERPL PENEFQRQVW LLFEYPESSG PARIIAIVSV MVILISIVSF 190 200 210 220 230 240 CLETLPIFRD ENEDMHGSGV TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR 250 260 270 280 290 300 FFACPSKAGF FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLVR 310 320 330 340 350 360 VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE ADERESQFPS 370 380 390 400 410 420 IPDAFWWAVV SMTTVGYGDM VPTTIGGKIV GSLCAIAGVL TIALPVPVIV SNFNYFYHRE 430 440 450 460 470 480 TEGEEQAQYL QVTSCPKIPS SPDLKKSRSA STISKSDYME IQEGVNNSNE DFREENLKTA 490 NCTLANTNYV NITKMLTDV
	Isoform 2 [UniParc]. Checksum: 9917ABBFCCCF7411	FASTA	356	40,966
	10 20 30 40 50 60 MTVATGDPAD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT LAQFPETLLG 70 80 90 100 110 120 DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR PVNVPLDIFS EEIRFYELGE 130 140 150 160 170 180 EAMEMFREDE GYIKEEERPL PENEFQRQVW LLFEYPESSG PARIIAIVSV MVILISIVSF 190 200 210 220 230 240 CLETLPIFRD ENEDMHGSGV TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR 250 260 270 280 290 300 FFACPSKAGF FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLER 310 320 330 340 350 RPLQSQKSKR GRQHLNTSHD CTLGINLVAG MTVQWTRASG PDDRQTPAVT TLHRMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human potassium channel genes: molecular cloning and functional expression." Ramaswami M., Gautam M., Kamb A., Rudy B., Tanouye M.A., Mathew M.K. Mol. Cell. Neurosci. 1:214-223(1990) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Blood.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02752 mRNA. Translation: AAA36141.1. AL365361 Genomic DNA. Translation: CAH71982.1. BC043564 mRNA. Translation: AAH43564.1.
IPI	IPI00028134. IPI00844248.
PIR	I77466.
RefSeq	NP_001191198.1. NM_001204269.1. NP_004965.1. NM_004974.3.
UniGene	Hs.248139. Hs.657199. Hs.731191.
3D structure databases
ProteinModelPortal	P16389.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000314520.
Protein family/group databases
TCDB	1.A.1.2.10. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	P16389.
Polymorphism databases
DMDM	1345813.
Proteomic databases
PaxDb	P16389.
PRIDE	P16389.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316361; ENSP00000314520; ENSG00000177301. ENST00000369770; ENSP00000358785; ENSG00000177301. ENST00000440270; ENSP00000415257; ENSG00000177301. ENST00000485317; ENSP00000433109; ENSG00000177301.
GeneID	3737.
KEGG	hsa:3737.
UCSC	uc009wfw.3. human.
Organism-specific databases
CTD	3737.
GeneCards	GC01M111137.
HGNC	HGNC:6220. KCNA2.
HPA	CAB001976.
MIM	176262. gene.
neXtProt	NX_P16389.
PharmGKB	PA206.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1226.
HOGENOM	HOG000231015.
HOVERGEN	HBG052230.
InParanoid	P16389.
KO	K04875.
OMA	AQYLQVN.
OrthoDB	EOG4DR9CB.
PhylomeDB	P16389.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
Bgee	P16389.
CleanEx	HS_KCNA2.
Genevestigator	P16389.
GermOnline	ENSG00000177301. Homo sapiens.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003972. K_chnl_volt-dep_Kv1. IPR004049. K_chnl_volt-dep_Kv1.2. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01509. KV12CHANNEL. PR01491. KVCHANNEL. PR01496. SHAKERCHANEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
BindingDB	P16389.
ChEMBL	CHEMBL2086.
GenomeRNAi	3737.
NextBio	14627.
SOURCE	Search...

Entry information

Entry name

KCNA2_HUMAN

Accession

Primary (citable) accession number: P16389
Secondary accession number(s): Q86XG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.