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P16388 (KCNA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily A member 1
Alternative name(s):
MBK1
MKI
Voltage-gated potassium channel subunit Kv1.1
Gene names
Name:Kcna1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure

Heterotetramer of potassium channel proteins. Binds KCNAB2 and PDZ domains of DLG1, DLG2 and DLG4 By similarity. Interacts with LGI1 within a complex containing LGI1, KCNA4 and KCNAB1 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Domain

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Post-translational modification

Palmitoylated on Cys-243; which may be required for membrane targeting By similarity.

Sequence similarities

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily. [View classification]

RNA editing

Edited at position 400.
Partially edited. RNA editing varies from 35% in the frontal cortex to 75% in the spinal chord. Ref.3

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Potassium voltage-gated channel subfamily A member 1
PRO_0000053969

Regions

Transmembrane168 – 18619Helical; Name=Segment S1; Potential
Transmembrane221 – 24222Helical; Name=Segment S2; Potential
Transmembrane254 – 27421Helical; Name=Segment S3; Potential
Transmembrane290 – 30920Helical; Voltage-sensor; Name=Segment S4; Potential
Transmembrane326 – 34520Helical; Name=Segment S5; Potential
Transmembrane387 – 40822Helical; Name=Segment S6; Potential
Motif372 – 3776Selectivity filter By similarity
Motif493 – 4953PDZ-binding By similarity

Amino acid modifications

Modified residue3221Phosphoserine; by PKA Potential
Modified residue4461Phosphoserine; by PKA Potential
Lipidation2431S-palmitoyl cysteine By similarity
Glycosylation2071N-linked (GlcNAc...) Potential

Natural variations

Natural variant4001I → V in RNA edited version.

Sequences

Sequence LengthMass (Da)Tools
P16388 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: C9249F130E943D3D

FASTA49556,409
        10         20         30         40         50         60 
MTVMSGENAD EASTAPGHPQ DGSYPRQADH DDHECCERVV INISGLRFET QLKTLAQFPN 

        70         80         90        100        110        120 
TLLGNPKKRM RYFDPLRNEY FFDRNRPSFD AILYYYQSGG RLRRPVNVPL DMFSEEIKFY 

       130        140        150        160        170        180 
ELGEEAMEKF REDEGFIKEE ERPLPEKEYQ RQVWLLFEYP ESSGPARVIA IVSVMVILIS 

       190        200        210        220        230        240 
IVIFCLETLP ELKDDKDFTG TIHRIDNTTV IYTSNIFTDP FFIVETLCII WFSFELVVRF 

       250        260        270        280        290        300 
FACPSKTDFF KNIMNFIDIV AIIPYFITLG TEIAEQEGNQ KGEQATSLAI LRVIRLVRVF 

       310        320        330        340        350        360 
RIFKLSRHSK GLQILGQTLK ASMRELGLLI FFLFIGVILF SSAVYFAEAE EAESHFSSIP 

       370        380        390        400        410        420 
DAFWWAVVSM TTVGYGDMYP VTIGGKIVGS LCAIAGVLTI ALPVPVIVSN FNYFYHRETE 

       430        440        450        460        470        480 
GEEQAQLLHV SSPNLASDSD LSRRSSSTIS KSEYMEIEED MNNSIAHYRQ ANIRTGNCTT 

       490 
ADQNCVNKSK LLTDV 

« Hide

References

« Hide 'large scale' references
[1]"A family of three mouse potassium channel genes with intronless coding regions."
Chandy K.G., Williams C.B., Spencer R.H., Aguilar B.A., Ghanshani S., Tempel B.L., Gutman G.A.
Science 247:973-975(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning of a probable potassium channel gene from mouse brain."
Tempel B.L., Jan Y.N., Jan L.Y.
Nature 332:837-839(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Nervous system targets of RNA editing identified by comparative genomics."
Hoopengardner B., Bhalla T., Staber C., Reenan R.
Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA EDITING OF POSITION 400.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30439 Genomic DNA. Translation: AAA39711.1.
Y00305 mRNA. Translation: CAA68408.1.
CCDSCCDS20555.1.
PIRA40090.
S09042.
RefSeqNP_034725.3. NM_010595.3.
UniGeneMm.40424.

3D structure databases

ProteinModelPortalP16388.
SMRP16388. Positions 36-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200876. 1 interaction.
IntActP16388. 2 interactions.
MINTMINT-4099639.

Chemistry

ChEMBLCHEMBL2429705.
GuidetoPHARMACOLOGY538.

PTM databases

PhosphoSiteP16388.

Proteomic databases

MaxQBP16388.
PaxDbP16388.
PRIDEP16388.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055168; ENSMUSP00000055225; ENSMUSG00000047976.
GeneID16485.
KEGGmmu:16485.
UCSCuc009dvb.1. mouse.

Organism-specific databases

CTD3736.
MGIMGI:96654. Kcna1.

Phylogenomic databases

eggNOGCOG1226.
GeneTreeENSGT00740000115256.
HOGENOMHOG000231015.
HOVERGENHBG052230.
InParanoidP16388.
KOK04874.
OMAEHDDHEC.
OrthoDBEOG7M0NRD.
PhylomeDBP16388.
TreeFamTF313103.

Gene expression databases

ArrayExpressP16388.
BgeeP16388.
GenevestigatorP16388.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004048. K_chnl_volt-dep_Kv1.1.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERPTHR11537. PTHR11537. 1 hit.
PfamPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR00169. KCHANNEL.
PR01508. KV11CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
ProtoNetSearch...

Other

NextBio289783.
PROP16388.
SOURCESearch...

Entry information

Entry nameKCNA1_MOUSE
AccessionPrimary (citable) accession number: P16388
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot