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P16388

- KCNA1_MOUSE

UniProt

P16388 - KCNA1_MOUSE

Protein

Potassium voltage-gated channel subfamily A member 1

Gene

Kcna1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome

    GO - Biological processi

    1. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199077. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 1
    Alternative name(s):
    MBK1
    MKI
    Voltage-gated potassium channel subunit Kv1.1
    Gene namesi
    Name:Kcna1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:96654. Kcna1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. dendrite Source: Ensembl
    3. juxtaparanode region of axon Source: BHF-UCL
    4. neuronal cell body Source: Ensembl
    5. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Potassium voltage-gated channel subfamily A member 1PRO_0000053969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
    Lipidationi243 – 2431S-palmitoyl cysteineBy similarity
    Modified residuei322 – 3221Phosphoserine; by PKASequence Analysis
    Modified residuei446 – 4461Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    Palmitoylated on Cys-243; which may be required for membrane targeting.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP16388.
    PaxDbiP16388.
    PRIDEiP16388.

    PTM databases

    PhosphoSiteiP16388.

    Expressioni

    Gene expression databases

    ArrayExpressiP16388.
    BgeeiP16388.
    GenevestigatoriP16388.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins. Binds KCNAB2 and PDZ domains of DLG1, DLG2 and DLG4 By similarity. Interacts with LGI1 within a complex containing LGI1, KCNA4 and KCNAB1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200876. 1 interaction.
    IntActiP16388. 2 interactions.
    MINTiMINT-4099639.

    Structurei

    3D structure databases

    ProteinModelPortaliP16388.
    SMRiP16388. Positions 36-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei168 – 18619Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei221 – 24222Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei290 – 30920Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei326 – 34520Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei387 – 40822Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi372 – 3776Selectivity filterBy similarity
    Motifi493 – 4953PDZ-bindingBy similarity

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115256.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiP16388.
    KOiK04874.
    OMAiEHDDHEC.
    OrthoDBiEOG7M0NRD.
    PhylomeDBiP16388.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004048. K_chnl_volt-dep_Kv1.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01508. KV11CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P16388-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVMSGENAD EASTAPGHPQ DGSYPRQADH DDHECCERVV INISGLRFET    50
    QLKTLAQFPN TLLGNPKKRM RYFDPLRNEY FFDRNRPSFD AILYYYQSGG 100
    RLRRPVNVPL DMFSEEIKFY ELGEEAMEKF REDEGFIKEE ERPLPEKEYQ 150
    RQVWLLFEYP ESSGPARVIA IVSVMVILIS IVIFCLETLP ELKDDKDFTG 200
    TIHRIDNTTV IYTSNIFTDP FFIVETLCII WFSFELVVRF FACPSKTDFF 250
    KNIMNFIDIV AIIPYFITLG TEIAEQEGNQ KGEQATSLAI LRVIRLVRVF 300
    RIFKLSRHSK GLQILGQTLK ASMRELGLLI FFLFIGVILF SSAVYFAEAE 350
    EAESHFSSIP DAFWWAVVSM TTVGYGDMYP VTIGGKIVGS LCAIAGVLTI 400
    ALPVPVIVSN FNYFYHRETE GEEQAQLLHV SSPNLASDSD LSRRSSSTIS 450
    KSEYMEIEED MNNSIAHYRQ ANIRTGNCTT ADQNCVNKSK LLTDV 495
    Length:495
    Mass (Da):56,409
    Last modified:August 1, 1990 - v1
    Checksum:iC9249F130E943D3D
    GO

    RNA editingi

    Partially edited. RNA editing varies from 35% in the frontal cortex to 75% in the spinal chord.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti400 – 4001I → V in RNA edited version.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30439 Genomic DNA. Translation: AAA39711.1.
    Y00305 mRNA. Translation: CAA68408.1.
    CCDSiCCDS20555.1.
    PIRiA40090.
    S09042.
    RefSeqiNP_034725.3. NM_010595.3.
    UniGeneiMm.40424.

    Genome annotation databases

    EnsembliENSMUST00000055168; ENSMUSP00000055225; ENSMUSG00000047976.
    GeneIDi16485.
    KEGGimmu:16485.
    UCSCiuc009dvb.1. mouse.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30439 Genomic DNA. Translation: AAA39711.1 .
    Y00305 mRNA. Translation: CAA68408.1 .
    CCDSi CCDS20555.1.
    PIRi A40090.
    S09042.
    RefSeqi NP_034725.3. NM_010595.3.
    UniGenei Mm.40424.

    3D structure databases

    ProteinModelPortali P16388.
    SMRi P16388. Positions 36-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200876. 1 interaction.
    IntActi P16388. 2 interactions.
    MINTi MINT-4099639.

    Chemistry

    ChEMBLi CHEMBL2429705.
    GuidetoPHARMACOLOGYi 538.

    PTM databases

    PhosphoSitei P16388.

    Proteomic databases

    MaxQBi P16388.
    PaxDbi P16388.
    PRIDEi P16388.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000055168 ; ENSMUSP00000055225 ; ENSMUSG00000047976 .
    GeneIDi 16485.
    KEGGi mmu:16485.
    UCSCi uc009dvb.1. mouse.

    Organism-specific databases

    CTDi 3736.
    MGIi MGI:96654. Kcna1.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115256.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi P16388.
    KOi K04874.
    OMAi EHDDHEC.
    OrthoDBi EOG7M0NRD.
    PhylomeDBi P16388.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199077. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 289783.
    PROi P16388.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P16388.
    Bgeei P16388.
    Genevestigatori P16388.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004048. K_chnl_volt-dep_Kv1.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01508. KV11CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A family of three mouse potassium channel genes with intronless coding regions."
      Chandy K.G., Williams C.B., Spencer R.H., Aguilar B.A., Ghanshani S., Tempel B.L., Gutman G.A.
      Science 247:973-975(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning of a probable potassium channel gene from mouse brain."
      Tempel B.L., Jan Y.N., Jan L.Y.
      Nature 332:837-839(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Nervous system targets of RNA editing identified by comparative genomics."
      Hoopengardner B., Bhalla T., Staber C., Reenan R.
      Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA EDITING OF POSITION 400.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNA1_MOUSE
    AccessioniPrimary (citable) accession number: P16388
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3