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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).2 Publications

Miscellaneous

Specific tRNA undermodification may be a switch to higher mutation frequency when cells are subject to environmental stresses other than treatment with mutagens.

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Strongly inhibited by ADP, ATP and other NTPs.1 Publication

Kineticsi

  1. KM=96 nM for tRNA(Phe)2 Publications
  2. KM=3.2 µM for DMAPP2 Publications

    pH dependencei

    Optimum pH is 6.5-9.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei280Required for specificity towards tRNA substrates containing a purine at position 291

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi17 – 24ATPSequence analysis8

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • tRNA dimethylallyltransferase activity Source: EcoCyc

    GO - Biological processi

    • cellular response to heat Source: EcoCyc
    • regulation of cytoplasmic translation in response to stress Source: EcoCyc
    • tRNA modification Source: EcoCyc

    Keywordsi

    Molecular functionTransferase
    Biological processtRNA processing
    LigandATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10595-MONOMER.
    MetaCyc:EG10595-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA dimethylallyltransferase (EC:2.5.1.75)
    Alternative name(s):
    Dimethylallyl diphosphate:tRNA dimethylallyltransferase
    Short name:
    DMAPP:tRNA dimethylallyltransferase
    Short name:
    DMATase
    Isopentenyl-diphosphate:tRNA isopentenyltransferase
    Short name:
    IPP transferase
    Short name:
    IPPT
    Short name:
    IPTase
    Gene namesi
    Name:miaA
    Synonyms:trpX
    Ordered Locus Names:b4171, JW4129
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10595. miaA.

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB02270. Dimethylallyl S-Thiolodiphosphate.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001639131 – 316tRNA dimethylallyltransferaseAdd BLAST316

    Proteomic databases

    PaxDbiP16384.
    PRIDEiP16384.

    Expressioni

    Inductioni

    By 2-aminopurine.

    Interactioni

    Subunit structurei

    Monomer. Binds to tRNA(Phe).3 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei108Interaction with substrate tRNA1
    Sitei130Interaction with substrate tRNA1

    Protein-protein interaction databases

    BioGridi4263002. 49 interactors.
    IntActiP16384. 5 interactors.
    STRINGi316385.ECDH10B_4366.

    Structurei

    Secondary structure

    1316
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi11 – 16Combined sources6
    Helixi23 – 33Combined sources11
    Beta strandi36 – 40Combined sources5
    Turni43 – 46Combined sources4
    Turni52 – 55Combined sources4
    Helixi59 – 64Combined sources6
    Beta strandi67 – 71Combined sources5
    Helixi81 – 97Combined sources17
    Beta strandi101 – 107Combined sources7
    Helixi109 – 116Combined sources8
    Helixi127 – 152Combined sources26
    Helixi154 – 159Combined sources6
    Helixi165 – 178Combined sources14
    Helixi182 – 186Combined sources5
    Beta strandi194 – 203Combined sources10
    Helixi207 – 223Combined sources17
    Helixi226 – 236Combined sources11
    Turni244 – 247Combined sources4
    Helixi251 – 259Combined sources9
    Helixi264 – 287Combined sources24
    Beta strandi293 – 296Combined sources4
    Helixi300 – 308Combined sources9

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2ZM5X-ray2.55A/B1-316[»]
    2ZXUX-ray2.75A/B1-316[»]
    3FOZX-ray2.50A/B1-316[»]
    ProteinModelPortaliP16384.
    SMRiP16384.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16384.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni19 – 24Substrate bindingBy similarity6
    Regioni42 – 45Interaction with substrate tRNA4
    Regioni120 – 124Interaction with substrate tRNA5
    Regioni166 – 170Interaction with substrate tRNA5
    Regioni206 – 229Interaction with isopentenylpyrophosphate transferaseAdd BLAST24
    Regioni247 – 252Interaction with substrate tRNA6
    Regioni280 – 287Interaction with substrate tRNA8

    Sequence similaritiesi

    Belongs to the IPP transferase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105DKX. Bacteria.
    COG0324. LUCA.
    HOGENOMiHOG000039996.
    InParanoidiP16384.
    KOiK00791.
    PhylomeDBiP16384.

    Family and domain databases

    HAMAPiMF_00185. IPP_trans. 1 hit.
    InterProiView protein in InterPro
    IPR018022. IPP_trans.
    IPR027417. P-loop_NTPase.
    PfamiView protein in Pfam
    PF01715. IPPT. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00174. miaA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P16384-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSDISKASLP KAIFLMGPTA SGKTALAIEL RKILPVELIS VDSALIYKGM
    60 70 80 90 100
    DIGTAKPNAE ELLAAPHRLL DIRDPSQAYS AADFRRDALA EMADITAAGR
    110 120 130 140 150
    IPLLVGGTML YFKALLEGLS PLPSADPEVR ARIEQQAAEQ GWESLHRQLQ
    160 170 180 190 200
    EVDPVAAARI HPNDPQRLSR ALEVFFISGK TLTELTQTSG DALPYQVHQF
    210 220 230 240 250
    AIAPASRELL HQRIEQRFHQ MLASGFEAEV RALFARGDLH TDLPSIRCVG
    260 270 280 290 300
    YRQMWSYLEG EISYDEMVYR GVCATRQLAK RQITWLRGWE GVHWLDSEKP
    310
    EQARDEVLQV VGAIAG
    Length:316
    Mass (Da):35,065
    Last modified:November 1, 1991 - v2
    Checksum:iFBFDC18770781C8F
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti101I → D in AAC43396 (Ref. 7) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M63655 Genomic DNA. Translation: AAA24174.1.
    U14003 Genomic DNA. Translation: AAA97067.1.
    U00096 Genomic DNA. Translation: AAC77128.1.
    AP009048 Genomic DNA. Translation: BAE78172.1.
    D00743 Genomic DNA. Translation: BAA00643.1.
    U00005 Unassigned DNA. Translation: AAC43396.1.
    Z11831 Genomic DNA. Translation: CAA77852.1.
    PIRiB37318.
    RefSeqiNP_418592.1. NC_000913.3.
    WP_001280345.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77128; AAC77128; b4171.
    BAE78172; BAE78172; BAE78172.
    GeneIDi948690.
    KEGGiecj:JW4129.
    eco:b4171.
    PATRICifig|1411691.4.peg.2530.

    Similar proteinsi

    Entry informationi

    Entry nameiMIAA_ECOLI
    AccessioniPrimary (citable) accession number: P16384
    Secondary accession number(s): Q2M6D4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: November 1, 1991
    Last modified: October 25, 2017
    This is version 152 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families