P16383 (GCFC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GC-rich sequence DNA-binding factor 2

Alternative name(s):
GC-rich sequence DNA-binding factor
Transcription factor 9
Short name=TCF-9

Gene names

Name:	GCFC2
Synonyms:	C2orf3, GCF, TCF9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	781 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.
Subcellular location	Nucleus.
Tissue specificity	Widely expressed in tissues and cell lines.
Sequence similarities	Belongs to the GCF family.
Sequence caution	The sequence AAA35598.1 differs from that shown. Reason: Frameshift at position 147. The sequence AAA35598.1 differs from that shown. Reason: Contaminating sequence. The N-terminus matches the 2q37.3 region.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Non-traceable author statement PubMed 10500253. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P16383-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P16383-2)

The sequence of this isoform differs from the canonical sequence as follows:
     169-207: GMKRESEDDPESEPDDHEKRIPFTLRPQTLRQRMAEESI → V

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 781	781	GC-rich sequence DNA-binding factor 2		PRO_0000087441
Regions
	Coiled coil	267 – 312	46	Potential
	Compositional bias	116 – 122	7	Poly-Ser
Amino acid modifications
	Modified residue	16	1	Phosphoserine Ref.5 Ref.6 Ref.9
	Modified residue	17	1	Phosphoserine Ref.5 Ref.6 Ref.9
	Modified residue	19	1	Phosphoserine Ref.5 Ref.6 Ref.9
	Modified residue	96	1	Phosphoserine Ref.7 Ref.9
	Modified residue	97	1	Phosphothreonine Ref.7 Ref.9
	Modified residue	129	1	Phosphoserine By similarity
	Modified residue	180	1	Phosphoserine By similarity
	Modified residue	213	1	Phosphothreonine Ref.5 Ref.6
	Modified residue	214	1	Phosphoserine Ref.5 Ref.6
	Modified residue	217	1	Phosphoserine Ref.5 Ref.6
Natural variations
	Alternative sequence	169 – 207	39	GMKRE…AEESI → V in isoform 2.		VSP_021798
	Natural variant	32	1	P → A. Ref.2 Corresponds to variant rs7559767 [ dbSNP | Ensembl ].		VAR_051005
	Natural variant	249	1	N → S. Corresponds to variant rs7560262 [ dbSNP | Ensembl ].		VAR_051006
	Natural variant	316	1	Q → E. Corresponds to variant rs6742946 [ dbSNP | Ensembl ].		VAR_051007
	Natural variant	594	1	T → A. Corresponds to variant rs6722682 [ dbSNP | Ensembl ].		VAR_051008
	Natural variant	724	1	E → D. Corresponds to variant rs17690300 [ dbSNP | Ensembl ].		VAR_051009

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 38D34EE4442EB3DF	FASTA	781	89,385
	10 20 30 40 50 60 MAHRPKRTFR QRAADSSDSD GAEESPAEPG APRELPVPGS AEEEPPSGGG RAQVAGLPHR 70 80 90 100 110 120 VRGPRGRGRV WASSRRATKA APRADEGSES RTLDVSTDEE DKIHHSSESK DDQGLSSDSS 130 140 150 160 170 180 SSLGEKELSS TVKIPDAAFI QAARRKRELA RAQDDYISLD VQHTSSISGM KRESEDDPES 190 200 210 220 230 240 EPDDHEKRIP FTLRPQTLRQ RMAEESISRN EETSEESQED EKQDTWEQQQ MRKAVKIIEE 250 260 270 280 290 300 RDIDLSCGNG SSKVKKFDTS ISFPPVNLEI IKKQLNTRLT LLQETHRSHL REYEKYVQDV 310 320 330 340 350 360 KSSKSTIQNL ESSSNQALNC KFYKSMKIYV ENLIDCLNEK IINIQEIESS MHALLLKQAM 370 380 390 400 410 420 TFMKRRQDEL KHESTYLQQL SRKDETSTSG NFSVDEKTQW ILEEIESRRT KRRQARVLSG 430 440 450 460 470 480 NCNHQEGTSS DDELPSAEMI DFQKSQGDIL QKQKKVFEEV QDDFCNIQNI LLKFQQWREK 490 500 510 520 530 540 FPDSYYEAFI SLCIPKLLNP LIRVQLIDWN PLKLESTGLK EMPWFKSVEE FMDSSVEDSK 550 560 570 580 590 600 KESSSDKKVL SAIINKTIIP RLTDFVEFLW DPLSTSQTTS LITHCRVILE EHSTCENEVS 610 620 630 640 650 660 KSRQDLLKSI VSRMKKAVED DVFIPLYPKS AVENKTSPHS KFQERQFWSG LKLFRNILLW 670 680 690 700 710 720 NGLLTDDTLQ ELGLGKLLNR YLIIALLNAT PGPDVVKKCN QVAACLPEKW FENSAMRTSI 730 740 750 760 770 780 PQLENFIQFL LQSAHKLSRS EFRDEVEEII LILVKIKALN QAESFIGEHH LDHLKSLIKE D
	Isoform 2 [UniParc]. Checksum: E0187138CD59CE75	FASTA	743	84,847
	10 20 30 40 50 60 MAHRPKRTFR QRAADSSDSD GAEESPAEPG APRELPVPGS AEEEPPSGGG RAQVAGLPHR 70 80 90 100 110 120 VRGPRGRGRV WASSRRATKA APRADEGSES RTLDVSTDEE DKIHHSSESK DDQGLSSDSS 130 140 150 160 170 180 SSLGEKELSS TVKIPDAAFI QAARRKRELA RAQDDYISLD VQHTSSISVS RNEETSEESQ 190 200 210 220 230 240 EDEKQDTWEQ QQMRKAVKII EERDIDLSCG NGSSKVKKFD TSISFPPVNL EIIKKQLNTR 250 260 270 280 290 300 LTLLQETHRS HLREYEKYVQ DVKSSKSTIQ NLESSSNQAL NCKFYKSMKI YVENLIDCLN 310 320 330 340 350 360 EKIINIQEIE SSMHALLLKQ AMTFMKRRQD ELKHESTYLQ QLSRKDETST SGNFSVDEKT 370 380 390 400 410 420 QWILEEIESR RTKRRQARVL SGNCNHQEGT SSDDELPSAE MIDFQKSQGD ILQKQKKVFE 430 440 450 460 470 480 EVQDDFCNIQ NILLKFQQWR EKFPDSYYEA FISLCIPKLL NPLIRVQLID WNPLKLESTG 490 500 510 520 530 540 LKEMPWFKSV EEFMDSSVED SKKESSSDKK VLSAIINKTI IPRLTDFVEF LWDPLSTSQT 550 560 570 580 590 600 TSLITHCRVI LEEHSTCENE VSKSRQDLLK SIVSRMKKAV EDDVFIPLYP KSAVENKTSP 610 620 630 640 650 660 HSKFQERQFW SGLKLFRNIL LWNGLLTDDT LQELGLGKLL NRYLIIALLN ATPGPDVVKK 670 680 690 700 710 720 CNQVAACLPE KWFENSAMRT SIPQLENFIQ FLLQSAHKLS RSEFRDEVEE IILILVKIKA 730 740 LNQAESFIGE HHLDHLKSLI KED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a human DNA binding factor that represses transcription." Kageyama R., Pastan I. Cell 59:815-825(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-32.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-17; SER-19; THR-213; SER-214 AND SER-217, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-17; SER-19; THR-213; SER-214 AND SER-217, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96 AND THR-97, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-17; SER-19; SER-96 AND THR-97, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29204 mRNA. Translation: AAA35598.1. Sequence problems. AC005034 Genomic DNA. Translation: AAY14973.1. BC064559 mRNA. Translation: AAH64559.1.
IPI	IPI00418340. IPI00807475.
PIR	A33633.
RefSeq	NP_001188263.1. NM_001201334.1. NP_003194.3. NM_003203.4.
UniGene	Hs.303808. Hs.662279. Hs.710597.
3D structure databases
ProteinModelPortal	P16383.
ModBase	Search...
Protein-protein interaction databases
IntAct	P16383. 4 interactions.
MINT	MINT-2863474.
STRING	9606.ENSP00000318690.
PTM databases
PhosphoSite	P16383.
Polymorphism databases
DMDM	118572650.
Proteomic databases
PaxDb	P16383.
PRIDE	P16383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000321027; ENSP00000318690; ENSG00000005436. ENST00000409857; ENSP00000386552; ENSG00000005436.
GeneID	6936.
KEGG	hsa:6936.
UCSC	uc002snn.3. human.
Organism-specific databases
CTD	6936.
GeneCards	GC02M075880.
HGNC	HGNC:1317. GCFC2.
MIM	189901. gene.
neXtProt	NX_P16383.
PharmGKB	PA25892.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG294271.
HOGENOM	HOG000112699.
HOVERGEN	HBG101878.
InParanoid	P16383.
KO	K09061.
OMA	QDTWEQQ.
OrthoDB	EOG415GDS.
PhylomeDB	P16383.
Gene expression databases
ArrayExpress	P16383.
Bgee	P16383.
CleanEx	HS_C2orf3.
Genevestigator	P16383.
GermOnline	ENSG00000005436. Homo sapiens.
Family and domain databases
InterPro	IPR012890. GCFC. IPR022783. GCFC_dom. [Graphical view]
PANTHER	PTHR12214. PTHR12214. 1 hit.
Pfam	PF07842. GCFC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6936.
NextBio	27141.
SOURCE	Search...

Entry information

Entry name

GCFC2_HUMAN

Accession

Primary (citable) accession number: P16383
Secondary accession number(s): O95032, Q53TY0, Q6P2F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 28, 2006
Last modified:	April 3, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.