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Protein

Putative ATP-dependent RNA helicase Pl10

Gene

D1Pas1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative ATP-dependent RNA helicase. Possible role in a key step of the spermatogenic process.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi199 – 2068ATPPROSITE-ProRule annotation
Nucleotide bindingi223 – 2308ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase Pl10 (EC:3.6.4.13)
Gene namesi
Name:D1Pas1
Synonyms:Pl10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:91842. D1Pas1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 660659Putative ATP-dependent RNA helicase Pl10PRO_0000055008Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei55 – 551N6-acetyllysineBy similarity
Modified residuei101 – 1011PhosphoserineBy similarity
Modified residuei103 – 1031PhosphotyrosineBy similarity
Modified residuei117 – 1171N6-acetyllysineBy similarity
Modified residuei182 – 1821PhosphoserineBy similarity
Cross-linki214 – 214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei455 – 4551PhosphoserineBy similarity
Modified residuei610 – 6101PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP16381.
PaxDbiP16381.
PRIDEiP16381.

PTM databases

iPTMnetiP16381.
PhosphoSiteiP16381.

Expressioni

Tissue specificityi

Testis.

Developmental stagei

High levels of PL10 during the meiotic and haploid stages of spermatogenesis.

Gene expression databases

BgeeiP16381.
CleanExiMM_D1PAS1.
ExpressionAtlasiP16381. baseline and differential.
GenevisibleiP16381. MM.

Interactioni

Protein-protein interaction databases

BioGridi226052. 2 interactions.
IntActiP16381. 1 interaction.
MINTiMINT-93268.
STRINGi10090.ENSMUSP00000035261.

Structurei

3D structure databases

ProteinModelPortaliP16381.
SMRiP16381. Positions 134-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini210 – 402193Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini413 – 574162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi179 – 20729Q motifAdd
BLAST
Motifi346 – 3494DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi581 – 66080Gly/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
HOGENOMiHOG000268804.
HOVERGENiHBG015893.
InParanoidiP16381.
KOiK11594.
OMAiMSHVAEE.
OrthoDBiEOG7B5WV8.
PhylomeDBiP16381.
TreeFamiTF300364.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHVAEEDEL GLDQQLAGLD LTSRDSQSGG STASKGRYIP PHLRNREAAK
60 70 80 90 100
AFYDKDGSRW SKDKDAYSSF GSRSDTRAKS SFFSDRGGSG SRGRFDERGR
110 120 130 140 150
SDYESVGSRG GRSGFGKFER GGNSRWCDKA DEDDWSKPLP PSERLEQELF
160 170 180 190 200
SGGNTGINFE KYDDIPVEAT GNNCPPHIES FSDVEMGEII MGNIELTRYT
210 220 230 240 250
RPTPVQKHAI PIIKEKRDLM ACAQTGSGKT AAFLLPILSQ IYTDGPGEAL
260 270 280 290 300
RAMKENGKYG RRKQYPISLV LAPTRELAVQ IYEEARKFSY RSRVRPCVVY
310 320 330 340 350
GGADIGQQIR DLERGCHLLV ATPGRLVDMM ERGKIGLDFC KYLVLDEADR
360 370 380 390 400
MLDMGFEPQI RRIVEQDTMP PKGVRHTMMF SATFPKEIQM LARDFLDEYI
410 420 430 440 450
FLAVGRVGST SENITQKVVW VEEADKRSFL LDLLNATGKD SLILVFVETK
460 470 480 490 500
KGADSLEDFL YHEGYACTSI HGDRSQRDRE EALHQFRSGK SPILVATAVA
510 520 530 540 550
ARGLDISNVK HVINFDLPSD IEEYVHRIGR TGRVGNLGLA TSFFNERNIN
560 570 580 590 600
ITKDLLDLLV EAKQEVPSWL ENMAFEHHYK GGSRGRSKSR FSGGFGARDY
610 620 630 640 650
RQSSGASSSS FSSGRASNSR SGGGSHGSSR GFGGGSYGGF YNSDGYGGNY
660
SSQGVDWWGN
Length:660
Mass (Da):73,141
Last modified:August 1, 1990 - v1
Checksum:i50AD4E6A131AE603
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04847 mRNA. Translation: AAA39942.1.
AK029542 mRNA. Translation: BAC26505.1.
CCDSiCCDS15603.1.
PIRiA32378.
RefSeqiNP_149068.1. NM_033077.3.
UniGeneiMm.108054.
Mm.487201.

Genome annotation databases

EnsembliENSMUST00000045108; ENSMUSP00000035261; ENSMUSG00000039224.
GeneIDi110957.
KEGGimmu:110957.
UCSCiuc007dzs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04847 mRNA. Translation: AAA39942.1.
AK029542 mRNA. Translation: BAC26505.1.
CCDSiCCDS15603.1.
PIRiA32378.
RefSeqiNP_149068.1. NM_033077.3.
UniGeneiMm.108054.
Mm.487201.

3D structure databases

ProteinModelPortaliP16381.
SMRiP16381. Positions 134-575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi226052. 2 interactions.
IntActiP16381. 1 interaction.
MINTiMINT-93268.
STRINGi10090.ENSMUSP00000035261.

PTM databases

iPTMnetiP16381.
PhosphoSiteiP16381.

Proteomic databases

EPDiP16381.
PaxDbiP16381.
PRIDEiP16381.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045108; ENSMUSP00000035261; ENSMUSG00000039224.
GeneIDi110957.
KEGGimmu:110957.
UCSCiuc007dzs.1. mouse.

Organism-specific databases

CTDi110957.
MGIiMGI:91842. D1Pas1.

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
HOGENOMiHOG000268804.
HOVERGENiHBG015893.
InParanoidiP16381.
KOiK11594.
OMAiMSHVAEE.
OrthoDBiEOG7B5WV8.
PhylomeDBiP16381.
TreeFamiTF300364.

Miscellaneous databases

PROiP16381.
SOURCEiSearch...

Gene expression databases

BgeeiP16381.
CleanExiMM_D1PAS1.
ExpressionAtlasiP16381. baseline and differential.
GenevisibleiP16381. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The protein encoded by a murine male germ cell-specific transcript is a putative ATP-dependent RNA helicase."
    Leroy P., Alzari P., Sassoon D., Wolgemuth D., Fellous M.
    Cell 57:549-559(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiDDX3L_MOUSE
AccessioniPrimary (citable) accession number: P16381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 8, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.