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Protein

G protein alpha o subunit

Gene

Galphao

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphai and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumBy similarity1
Metal bindingi182MagnesiumBy similarity1
Binding sitei326GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47GTPBy similarity8
Nucleotide bindingi176 – 182GTPBy similarity7
Nucleotide bindingi201 – 205GTPBy similarity5
Nucleotide bindingi270 – 273GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • asymmetric cell division Source: FlyBase
  • axon ensheathment in central nervous system Source: UniProtKB
  • behavioral response to starvation Source: FlyBase
  • calcium-mediated signaling Source: FlyBase
  • cell adhesion involved in heart morphogenesis Source: FlyBase
  • cortical actin cytoskeleton organization Source: UniProtKB
  • establishment of endothelial blood-brain barrier Source: UniProtKB
  • establishment of glial blood-brain barrier Source: FlyBase
  • establishment of imaginal disc-derived wing hair orientation Source: FlyBase
  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • heart development Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • non-canonical Wnt signaling pathway Source: FlyBase
  • sensory perception of sweet taste Source: FlyBase
  • septate junction assembly Source: FlyBase
  • ventral cord development Source: FlyBase

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-112043 PLC beta mediated events
R-DME-202040 G-protein activation

Names & Taxonomyi

Protein namesi
Recommended name:
G protein alpha o subunit
Alternative name(s):
Guanine nucleotide-binding protein G(o) subunit alpha 47A
Gene namesi
Name:Galphao
Synonyms:G-oa47A, G-oalpha47A
ORF Names:CG2204
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001122 Galphao

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002037162 – 354G protein alpha o subunitAdd BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Lipidationi3S-palmitoyl cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiP16378
PRIDEiP16378

Expressioni

Tissue specificityi

Expressed primarily in neuronal cell bodies in the brain, optic lobe, and thoracic and abdominal ganglia. Also expressed in antenna, oocytes and ovarian nurse cells.4 Publications

Developmental stagei

Expressed in the surface glial cells of the nerve cords at the larval stage (at protein level). Expressed throughout development.2 Publications

Gene expression databases

BgeeiFBgn0001122
ExpressionAtlasiP16378 baseline and differential
GenevisibleiP16378 DM

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Binary interactionsi

WithEntry#Exp.IntActNotes
pinsQ9VB222EBI-197464,EBI-116643

GO - Molecular functioni

Protein-protein interaction databases

BioGridi61922, 29 interactors
DIPiDIP-17224N
IntActiP16378, 6 interactors
STRINGi7227.FBpp0087359

Structurei

3D structure databases

ProteinModelPortaliP16378
SMRiP16378
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
InParanoidiP16378
KOiK04534
OMAiVARMEDT
OrthoDBiEOG091G0VUT
PhylomeDBiP16378

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P16378-1) [UniParc]FASTAAdd to basket
Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCAQSAEER AAAARSRLIE RNLKEDGIQA AKDIKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHESG FTAEDFKQYR PVVYSNTIQS LVAILRAMPT LSIQYSNNER
110 120 130 140 150
ESDAKMVFDV CQRMHDTEPF SEELLAAMKR LWQDAGVQEC FSRSNEYQLN
160 170 180 190 200
DSAKYFLDDL DRLGAKDYQP TEQDILRTRV KTTGIVEVHF SFKNLNFKLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVAMSE YDQVLHEDET TNRMQESLKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIR KSPLTICFPE YTGGQEYGEA
310 320 330 340 350
AAYIQAQFEA KNKSTSKEIY CHMTCATDTN NIQFVFDAVT DVIIANNLRG

CGLY
Length:354
Mass (Da):40,476
Last modified:August 1, 1990 - v1
Checksum:i3C5DA142B4CF7DD2
GO
Isoform B (identifier: P16378-2) [UniParc]FASTAAdd to basket
Also known as: D, E, F, G, H

The sequence of this isoform differs from the canonical sequence as follows:
     4-21: AQSAEERAAAARSRLIER → TTSAEERAAIQRSKQIEK

Show »
Length:354
Mass (Da):40,537
Checksum:i5C80ACDDB18FF6BF
GO

Sequence cautioni

The sequence AAN71523 differs from that shown. Reason: Erroneous termination at position 44. Translated as Ser.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88M → I in AAA28585 (PubMed:2509463).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0018304 – 21AQSAE…RLIER → TTSAEERAAIQRSKQIEK in isoform B. 4 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86660 mRNA Translation: AAA28577.1
M29602 mRNA Translation: AAA28587.1
M31203
, M29601, M31198, M31199, M31200, M31201, M31202 Genomic DNA Translation: AAA28586.1
M30151 mRNA Translation: AAA28584.1
M30152 mRNA Translation: AAA28585.1
M31129 Genomic DNA Translation: AAA28583.1
M29731 mRNA Translation: AAA28580.1
M29732 mRNA Translation: AAA28581.1
AE013599 Genomic DNA Translation: AAF58789.1
AE013599 Genomic DNA Translation: AAF58790.1
AE013599 Genomic DNA Translation: AAO41420.1
AE013599 Genomic DNA Translation: AAO41421.1
AE013599 Genomic DNA Translation: AAO41422.1
AE013599 Genomic DNA Translation: AAO41423.1
AE013599 Genomic DNA Translation: AAS64872.1
AE013599 Genomic DNA Translation: AAS64873.1
AY121631 mRNA Translation: AAM51958.1
BT001768 mRNA Translation: AAN71523.1 Sequence problems.
BT046134 mRNA Translation: ACK77603.1
PIRiA34304 RGFFO1
B34304 RGFFO2
RefSeqiNP_523684.2, NM_078960.5 [P16378-1]
NP_724934.1, NM_165772.4 [P16378-2]
NP_724935.1, NM_165773.3 [P16378-1]
NP_788304.1, NM_176124.3 [P16378-2]
NP_788305.1, NM_176125.3 [P16378-2]
NP_788306.1, NM_176126.3 [P16378-2]
NP_788307.1, NM_176127.3 [P16378-2]
NP_995801.1, NM_206079.2 [P16378-1]
NP_995802.1, NM_206080.2 [P16378-2]
UniGeneiDm.12982

Genome annotation databases

EnsemblMetazoaiFBtr0088264; FBpp0087359; FBgn0001122 [P16378-2]
FBtr0088265; FBpp0087360; FBgn0001122 [P16378-1]
FBtr0088266; FBpp0087361; FBgn0001122 [P16378-1]
FBtr0088267; FBpp0087362; FBgn0001122 [P16378-2]
FBtr0088268; FBpp0087363; FBgn0001122 [P16378-2]
FBtr0088269; FBpp0087364; FBgn0001122 [P16378-2]
FBtr0088270; FBpp0087365; FBgn0001122 [P16378-2]
FBtr0088271; FBpp0089315; FBgn0001122 [P16378-2]
FBtr0088272; FBpp0089316; FBgn0001122 [P16378-1]
GeneIDi36104
KEGGidme:Dmel_CG2204

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGNAO_DROME
AccessioniPrimary (citable) accession number: P16378
Secondary accession number(s): A4UZB6
, A4UZC1, B7FF70, P16377, P16707, Q540V8, Q8IGI5, Q9V5L5, Q9V5L6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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