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Protein

G protein alpha o subunit

Gene

Galphao

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphai and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity
Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi176 – 1827GTPBy similarity
Nucleotide bindingi201 – 2055GTPBy similarity
Nucleotide bindingi270 – 2734GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • asymmetric cell division Source: FlyBase
  • axon ensheathment in central nervous system Source: UniProtKB
  • behavioral response to starvation Source: FlyBase
  • calcium-mediated signaling Source: FlyBase
  • cell adhesion involved in heart morphogenesis Source: FlyBase
  • cortical actin cytoskeleton organization Source: UniProtKB
  • establishment of endothelial blood-brain barrier Source: UniProtKB
  • establishment of glial blood-brain barrier Source: FlyBase
  • establishment of imaginal disc-derived wing hair orientation Source: FlyBase
  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • heart development Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • sensory perception of sweet taste Source: FlyBase
  • septate junction assembly Source: FlyBase
  • ventral cord development Source: FlyBase
  • Wnt signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-112043. PLC beta mediated events.
R-DME-202040. G-protein activation.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein alpha o subunit
Alternative name(s):
Guanine nucleotide-binding protein G(o) subunit alpha 47A
Gene namesi
Name:Galphao
Synonyms:G-oa47A, G-oalpha47A
ORF Names:CG2204
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001122. Galphao.

Subcellular locationi

GO - Cellular componenti

  • heterotrimeric G-protein complex Source: FlyBase
  • plasma membrane Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 354353G protein alpha o subunitPRO_0000203716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Lipidationi3 – 31S-palmitoyl cysteineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiP16378.
PRIDEiP16378.

Expressioni

Tissue specificityi

Expressed primarily in neuronal cell bodies in the brain, optic lobe, and thoracic and abdominal ganglia. Also expressed in antenna, oocytes and ovarian nurse cells.4 Publications

Developmental stagei

Expressed in the surface glial cells of the nerve cords at the larval stage (at protein level). Expressed throughout development.2 Publications

Gene expression databases

BgeeiFBgn0001122.
ExpressionAtlasiP16378. differential.
GenevisibleiP16378. DM.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi61922. 12 interactions.
DIPiDIP-17224N.
IntActiP16378. 6 interactions.
STRINGi7227.FBpp0087359.

Structurei

3D structure databases

ProteinModelPortaliP16378.
SMRiP16378. Positions 35-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
InParanoidiP16378.
KOiK04534.
OMAiNNEREPD.
OrthoDBiEOG091G0VUT.
PhylomeDBiP16378.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P16378-1) [UniParc]FASTAAdd to basket
Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCAQSAEER AAAARSRLIE RNLKEDGIQA AKDIKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHESG FTAEDFKQYR PVVYSNTIQS LVAILRAMPT LSIQYSNNER
110 120 130 140 150
ESDAKMVFDV CQRMHDTEPF SEELLAAMKR LWQDAGVQEC FSRSNEYQLN
160 170 180 190 200
DSAKYFLDDL DRLGAKDYQP TEQDILRTRV KTTGIVEVHF SFKNLNFKLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVAMSE YDQVLHEDET TNRMQESLKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIR KSPLTICFPE YTGGQEYGEA
310 320 330 340 350
AAYIQAQFEA KNKSTSKEIY CHMTCATDTN NIQFVFDAVT DVIIANNLRG

CGLY
Length:354
Mass (Da):40,476
Last modified:August 1, 1990 - v1
Checksum:i3C5DA142B4CF7DD2
GO
Isoform B (identifier: P16378-2) [UniParc]FASTAAdd to basket
Also known as: D, E, F, G, H

The sequence of this isoform differs from the canonical sequence as follows:
     4-21: AQSAEERAAAARSRLIER → TTSAEERAAIQRSKQIEK

Show »
Length:354
Mass (Da):40,537
Checksum:i5C80ACDDB18FF6BF
GO

Sequence cautioni

The sequence AAN71523 differs from that shown. Reason: Erroneous termination at position 44. Translated as Ser.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881M → I in AAA28585 (PubMed:2509463).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei4 – 2118AQSAE…RLIER → TTSAEERAAIQRSKQIEK in isoform B. 4 PublicationsVSP_001830Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86660 mRNA. Translation: AAA28577.1.
M29602 mRNA. Translation: AAA28587.1.
M31203
, M29601, M31198, M31199, M31200, M31201, M31202 Genomic DNA. Translation: AAA28586.1.
M30151 mRNA. Translation: AAA28584.1.
M30152 mRNA. Translation: AAA28585.1.
M31129 Genomic DNA. Translation: AAA28583.1.
M29731 mRNA. Translation: AAA28580.1.
M29732 mRNA. Translation: AAA28581.1.
AE013599 Genomic DNA. Translation: AAF58789.1.
AE013599 Genomic DNA. Translation: AAF58790.1.
AE013599 Genomic DNA. Translation: AAO41420.1.
AE013599 Genomic DNA. Translation: AAO41421.1.
AE013599 Genomic DNA. Translation: AAO41422.1.
AE013599 Genomic DNA. Translation: AAO41423.1.
AE013599 Genomic DNA. Translation: AAS64872.1.
AE013599 Genomic DNA. Translation: AAS64873.1.
AY121631 mRNA. Translation: AAM51958.1.
BT001768 mRNA. Translation: AAN71523.1. Sequence problems.
BT046134 mRNA. Translation: ACK77603.1.
PIRiA34304. RGFFO1.
B34304. RGFFO2.
RefSeqiNP_523684.2. NM_078960.5. [P16378-1]
NP_724934.1. NM_165772.4. [P16378-2]
NP_724935.1. NM_165773.3. [P16378-1]
NP_788304.1. NM_176124.3. [P16378-2]
NP_788305.1. NM_176125.3. [P16378-2]
NP_788306.1. NM_176126.3. [P16378-2]
NP_788307.1. NM_176127.3. [P16378-2]
NP_995801.1. NM_206079.2. [P16378-1]
NP_995802.1. NM_206080.2. [P16378-2]
UniGeneiDm.12982.

Genome annotation databases

EnsemblMetazoaiFBtr0088265; FBpp0087360; FBgn0001122. [P16378-1]
FBtr0088266; FBpp0087361; FBgn0001122. [P16378-1]
FBtr0088272; FBpp0089316; FBgn0001122. [P16378-1]
GeneIDi36104.
KEGGidme:Dmel_CG2204.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86660 mRNA. Translation: AAA28577.1.
M29602 mRNA. Translation: AAA28587.1.
M31203
, M29601, M31198, M31199, M31200, M31201, M31202 Genomic DNA. Translation: AAA28586.1.
M30151 mRNA. Translation: AAA28584.1.
M30152 mRNA. Translation: AAA28585.1.
M31129 Genomic DNA. Translation: AAA28583.1.
M29731 mRNA. Translation: AAA28580.1.
M29732 mRNA. Translation: AAA28581.1.
AE013599 Genomic DNA. Translation: AAF58789.1.
AE013599 Genomic DNA. Translation: AAF58790.1.
AE013599 Genomic DNA. Translation: AAO41420.1.
AE013599 Genomic DNA. Translation: AAO41421.1.
AE013599 Genomic DNA. Translation: AAO41422.1.
AE013599 Genomic DNA. Translation: AAO41423.1.
AE013599 Genomic DNA. Translation: AAS64872.1.
AE013599 Genomic DNA. Translation: AAS64873.1.
AY121631 mRNA. Translation: AAM51958.1.
BT001768 mRNA. Translation: AAN71523.1. Sequence problems.
BT046134 mRNA. Translation: ACK77603.1.
PIRiA34304. RGFFO1.
B34304. RGFFO2.
RefSeqiNP_523684.2. NM_078960.5. [P16378-1]
NP_724934.1. NM_165772.4. [P16378-2]
NP_724935.1. NM_165773.3. [P16378-1]
NP_788304.1. NM_176124.3. [P16378-2]
NP_788305.1. NM_176125.3. [P16378-2]
NP_788306.1. NM_176126.3. [P16378-2]
NP_788307.1. NM_176127.3. [P16378-2]
NP_995801.1. NM_206079.2. [P16378-1]
NP_995802.1. NM_206080.2. [P16378-2]
UniGeneiDm.12982.

3D structure databases

ProteinModelPortaliP16378.
SMRiP16378. Positions 35-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61922. 12 interactions.
DIPiDIP-17224N.
IntActiP16378. 6 interactions.
STRINGi7227.FBpp0087359.

Proteomic databases

PaxDbiP16378.
PRIDEiP16378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088265; FBpp0087360; FBgn0001122. [P16378-1]
FBtr0088266; FBpp0087361; FBgn0001122. [P16378-1]
FBtr0088272; FBpp0089316; FBgn0001122. [P16378-1]
GeneIDi36104.
KEGGidme:Dmel_CG2204.

Organism-specific databases

CTDi36104.
FlyBaseiFBgn0001122. Galphao.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
InParanoidiP16378.
KOiK04534.
OMAiNNEREPD.
OrthoDBiEOG091G0VUT.
PhylomeDBiP16378.

Enzyme and pathway databases

ReactomeiR-DME-112043. PLC beta mediated events.
R-DME-202040. G-protein activation.

Miscellaneous databases

ChiTaRSiG-oalpha47A. fly.
GenomeRNAii36104.
PROiP16378.

Gene expression databases

BgeeiFBgn0001122.
ExpressionAtlasiP16378. differential.
GenevisibleiP16378. DM.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNAO_DROME
AccessioniPrimary (citable) accession number: P16378
Secondary accession number(s): A4UZB6
, A4UZC1, B7FF70, P16377, P16707, Q540V8, Q8IGI5, Q9V5L5, Q9V5L6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 7, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.