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Protein

Protein groucho

Gene

gro

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that regulates transcription when recruited to specific target DNA by hairy-related bHLH proteins. Maternally required for neurogenesis; in the segregation of the neuroectoderm. Directly or indirectly interacts with Notch and Delta.1 Publication

GO - Molecular functioni

  • CRD domain binding Source: UniProtKB
  • HMG box domain binding Source: UniProtKB
  • repressing transcription factor binding Source: FlyBase
  • RNA polymerase II transcription corepressor activity Source: FlyBase
  • transcription corepressor activity Source: UniProtKB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • nervous system development Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • sex determination, primary response to X:A ratio Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: Reactome

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-DME-201722 Formation of the beta-catenin:TCF transactivating complex
R-DME-209421 Transcription activation by ARM
R-DME-209441 WG ligand not bound to FZ receptors
R-DME-3769402 Deactivation of the beta-catenin transactivating complex
R-DME-4641265 Repression of WNT target genes
SignaLinkiP16371

Names & Taxonomyi

Protein namesi
Recommended name:
Protein groucho
Alternative name(s):
Enhancer of split m9/10 protein
Short name:
E(spl)m9/10
Gene namesi
Name:gro
Synonyms:E(spl)m9/m10
ORF Names:CG8384
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0001139 gro

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510101 – 730Protein grouchoAdd BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205Phosphoserine1 Publication1
Modified residuei207Phosphoserine1 Publication1
Modified residuei218Phosphoserine1 Publication1
Modified residuei242Phosphoserine; by CK2Sequence analysis1
Modified residuei258Phosphoserine; by CDC2Sequence analysis1
Modified residuei267Phosphoserine1 Publication1
Modified residuei326Phosphothreonine1 Publication1
Modified residuei328Phosphothreonine1 Publication1

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP16371
PRIDEiP16371

PTM databases

iPTMnetiP16371

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0001139
ExpressionAtlasiP16371 baseline and differential

Interactioni

Subunit structurei

Forms a complex with the hairy/Enhancer of split/deadpan family of basic helix-loop-helix proteins in order to repress transcription. Its activity in regulating transcription depends on other proteins as it lacks a DNA-binding motif.

Binary interactionsi

Show more details

GO - Molecular functioni

  • CRD domain binding Source: UniProtKB
  • HMG box domain binding Source: UniProtKB
  • repressing transcription factor binding Source: FlyBase
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi68062, 65 interactors
DIPiDIP-40N
ELMiP16371
IntActiP16371, 24 interactors
MINTiP16371
STRINGi7227.FBpp0089115

Structurei

3D structure databases

ProteinModelPortaliP16371
SMRiP16371
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati442 – 480WD 1Add BLAST39
Repeati488 – 527WD 2Add BLAST40
Repeati532 – 571WD 3Add BLAST40
Repeati574 – 613WD 4Add BLAST40
Repeati615 – 654WD 5Add BLAST40
Repeati656 – 695WD 6Add BLAST40
Repeati697 – 730WD 7Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 267CCN domainAdd BLAST62
Regioni262 – 425Binding to basic helix-loop-helix domainAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi227 – 230Nuclear localization signalSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi93 – 144Gln-richAdd BLAST52
Compositional biasi317 – 423Pro-richAdd BLAST107

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639 Eukaryota
ENOG410XPX3 LUCA
GeneTreeiENSGT00550000074465
InParanoidiP16371
KOiK04497
OrthoDBiEOG091G181Y
PhylomeDBiP16371

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR005617 Groucho/TLE_N
IPR009146 Groucho_enhance
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF03920 TLE_N, 1 hit
PF00400 WD40, 3 hits
PRINTSiPR01850 GROUCHOFAMLY
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform E (identifier: P16371-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYPSPVRHPA AGGPPPQGPI KFTIADTLER IKEEFNFLQA QYHSIKLECE
60 70 80 90 100
KLSNEKTEMQ RHYVMYYEMS YGLNVEMHKQ TEIAKRLNTL INQLLPFLQA
110 120 130 140 150
DHQQQVLQAV ERAKQVTMQE LNLIIGHQQQ HGIQQLLQQI HAQQVPGGPP
160 170 180 190 200
QPMGALNPFG ALGATMGLPH GPQGLLNKPP EHHRPDIKPT GLEGPAAAEE
210 220 230 240 250
RLRNSVSPAD REKYRTRSPL DIENDSKRRK DEKLQEDEGE KSDQDLVVDV
260 270 280 290 300
ANEMESHSPR PNGEHVSMEV RDRESLNGER LEKPSSSGIK QERPPSRSGS
310 320 330 340 350
SSSRSTPSLK TKDMEKPGTP GAKARTPTPN AAAPAPGVNP KQMMPQGPPP
360 370 380 390 400
AGYPGAPYQR PADPYQRPPS DPAYGRPPPM PYDPHAHVRT NGIPHPSALT
410 420 430 440 450
GGKPAYSFHM NGEGSLQPVP FPPDALVGVG IPRHARQINT LSHGEVVCAV
460 470 480 490 500
TISNPTKYVY TGGKGCVKVW DISQPGNKNP VSQLDCLQRD NYIRSVKLLP
510 520 530 540 550
DGRTLIVGGE ASNLSIWDLA SPTPRIKAEL TSAAPACYAL AISPDSKVCF
560 570 580 590 600
SCCSDGNIAV WDLHNEILVR QFQGHTDGAS CIDISPDGSR LWTGGLDNTV
610 620 630 640 650
RSWDLREGRQ LQQHDFSSQI FSLGYCPTGD WLAVGMENSH VEVLHASKPD
660 670 680 690 700
KYQLHLHESC VLSLRFAACG KWFVSTGKDN LLNAWRTPYG ASIFQSKETS
710 720 730
SVLSCDISTD DKYIVTGSGD KKATVYEVIY
Note: No experimental confirmation available.
Length:730
Mass (Da):80,231
Last modified:January 9, 2007 - v3
Checksum:i9DA08E1ED343B2F5
GO
Isoform A (identifier: P16371-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     127-137: Missing.

Show »
Length:719
Mass (Da):78,920
Checksum:iD495291FD77026A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41Q → H in AAA28512 (PubMed:3142687).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022308127 – 137Missing in isoform A. 2 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20571 mRNA Translation: AAA28512.1
AE014297 Genomic DNA Translation: AAF56557.1
AE014297 Genomic DNA Translation: AAN14068.1
AE014297 Genomic DNA Translation: AAN14069.1
AE014297 Genomic DNA Translation: AAS65218.1
AF145695 mRNA Translation: AAD38670.1
PIRiA30047
RefSeqiNP_001189293.1, NM_001202364.1 [P16371-2]
NP_524514.2, NM_079790.5 [P16371-2]
NP_733133.1, NM_170254.3 [P16371-2]
NP_733134.1, NM_170255.4 [P16371-2]
NP_733135.1, NM_170256.2 [P16371-2]
NP_996298.1, NM_206575.3 [P16371-1]
UniGeneiDm.1340

Genome annotation databases

EnsemblMetazoaiFBtr0084962; FBpp0084336; FBgn0001139 [P16371-2]
FBtr0084963; FBpp0084337; FBgn0001139 [P16371-2]
FBtr0084964; FBpp0084338; FBgn0001139 [P16371-2]
FBtr0084965; FBpp0084339; FBgn0001139 [P16371-2]
FBtr0084966; FBpp0089115; FBgn0001139 [P16371-1]
FBtr0302951; FBpp0292077; FBgn0001139 [P16371-2]
GeneIDi43162
KEGGidme:Dmel_CG8384
UCSCiCG8384-RA d. melanogaster

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGROU_DROME
AccessioniPrimary (citable) accession number: P16371
Secondary accession number(s): A4V3F6
, Q0KI08, Q7KRZ4, Q9V3F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 188 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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