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P16370

- RPB3_YEAST

UniProt

P16370 - RPB3_YEAST

Protein

DNA-directed RNA polymerase II subunit RPB3

Gene

RPB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Seems to be involved in transcription termination.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi86 – 861Zinc
    Metal bindingi88 – 881Zinc
    Metal bindingi92 – 921Zinc
    Metal bindingi95 – 951Zinc

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct

    GO - Biological processi

    1. termination of RNA polymerase II transcription Source: SGD
    2. transcription, RNA-templated Source: GOC
    3. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31296-MONOMER.
    ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB3
    Short name:
    RNA polymerase II subunit 3
    Short name:
    RNA polymerase II subunit B3
    Alternative name(s):
    B44.5
    DNA-directed RNA polymerase II 45 kDa polypeptide
    Gene namesi
    Name:RPB3
    Ordered Locus Names:YIL021W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL021w.
    SGDiS000001283. RPB3.

    Subcellular locationi

    GO - Cellular componenti

    1. DNA-directed RNA polymerase II, core complex Source: SGD
    2. nucleoplasm Source: SGD

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi9 – 91K → E: Transcript termination readthrough. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 318317DNA-directed RNA polymerase II subunit RPB3PRO_0000132748Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP16370.
    PaxDbiP16370.
    PeptideAtlasiP16370.

    Expressioni

    Gene expression databases

    GenevestigatoriP16370.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RPB11P389024EBI-15773,EBI-15806
    RPB2P085185EBI-15773,EBI-15767

    Protein-protein interaction databases

    BioGridi34967. 194 interactions.
    DIPiDIP-837N.
    IntActiP16370. 32 interactions.
    MINTiMINT-613688.
    STRINGi4932.YIL021W.

    Structurei

    Secondary structure

    1
    318
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 73
    Beta strandi11 – 133
    Beta strandi15 – 184
    Beta strandi21 – 255
    Helixi27 – 3913
    Beta strandi43 – 5412
    Beta strandi56 – 583
    Helixi60 – 689
    Beta strandi72 – 743
    Helixi77 – 793
    Turni83 – 853
    Beta strandi86 – 905
    Turni93 – 953
    Beta strandi96 – 1049
    Beta strandi107 – 1093
    Beta strandi111 – 1144
    Helixi115 – 1173
    Beta strandi118 – 1203
    Beta strandi128 – 1325
    Beta strandi137 – 1393
    Beta strandi142 – 1476
    Beta strandi152 – 16211
    Turni164 – 1663
    Helixi168 – 1703
    Beta strandi173 – 1808
    Helixi184 – 1863
    Helixi197 – 2004
    Helixi205 – 2073
    Beta strandi208 – 2103
    Beta strandi215 – 2173
    Beta strandi228 – 2347
    Beta strandi236 – 2383
    Helixi240 – 26526

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I3QX-ray3.10C1-318[»]
    1I50X-ray2.80C1-318[»]
    1I6HX-ray3.30C1-318[»]
    1K83X-ray2.80C1-318[»]
    1NIKX-ray4.10C1-318[»]
    1NT9X-ray4.20C1-318[»]
    1PQVX-ray3.80C1-318[»]
    1R5UX-ray4.50C1-318[»]
    1R9SX-ray4.25C1-318[»]
    1R9TX-ray3.50C1-318[»]
    1SFOX-ray3.61C1-318[»]
    1TWAX-ray3.20C1-318[»]
    1TWCX-ray3.00C1-318[»]
    1TWFX-ray2.30C1-318[»]
    1TWGX-ray3.30C1-318[»]
    1TWHX-ray3.40C1-318[»]
    1WCMX-ray3.80C1-318[»]
    1Y1VX-ray3.80C1-318[»]
    1Y1WX-ray4.00C1-318[»]
    1Y1YX-ray4.00C1-318[»]
    1Y77X-ray4.50C1-318[»]
    2B63X-ray3.80C1-318[»]
    2B8KX-ray4.15C1-318[»]
    2E2HX-ray3.95C1-318[»]
    2E2IX-ray3.41C1-318[»]
    2E2JX-ray3.50C1-318[»]
    2JA5X-ray3.80C1-318[»]
    2JA6X-ray4.00C1-318[»]
    2JA7X-ray3.80C/O1-318[»]
    2JA8X-ray3.80C1-318[»]
    2NVQX-ray2.90C1-318[»]
    2NVTX-ray3.36C1-318[»]
    2NVXX-ray3.60C1-318[»]
    2NVYX-ray3.40C1-318[»]
    2NVZX-ray4.30C1-318[»]
    2R7ZX-ray3.80C1-318[»]
    2R92X-ray3.80C1-318[»]
    2R93X-ray4.00C1-318[»]
    2VUMX-ray3.40C1-318[»]
    2YU9X-ray3.40C1-318[»]
    3CQZX-ray2.80C1-318[»]
    3FKIX-ray3.88C1-318[»]
    3GTGX-ray3.78C1-318[»]
    3GTJX-ray3.42C1-318[»]
    3GTKX-ray3.80C1-318[»]
    3GTLX-ray3.38C1-318[»]
    3GTMX-ray3.80C1-318[»]
    3GTOX-ray4.00C1-318[»]
    3GTPX-ray3.90C1-318[»]
    3GTQX-ray3.80C1-318[»]
    3H3VX-ray4.00D1-318[»]
    3HOUX-ray3.20C/O1-318[»]
    3HOVX-ray3.50C1-318[»]
    3HOWX-ray3.60C2-318[»]
    3HOXX-ray3.65C2-318[»]
    3HOYX-ray3.40C2-318[»]
    3HOZX-ray3.65C2-318[»]
    3I4MX-ray3.70C1-318[»]
    3I4NX-ray3.90C1-318[»]
    3J0Kelectron microscopy36.00C1-268[»]
    3J1Nelectron microscopy16.00C1-268[»]
    3K1FX-ray4.30C1-318[»]
    3K7AX-ray3.80C1-318[»]
    3M3YX-ray3.18C1-318[»]
    3M4OX-ray3.57C1-318[»]
    3PO2X-ray3.30C1-318[»]
    3PO3X-ray3.30C1-318[»]
    3QT1X-ray4.30C1-318[»]
    3RZDX-ray3.30C1-318[»]
    3RZOX-ray3.00C1-318[»]
    3S14X-ray2.85C1-318[»]
    3S15X-ray3.30C1-318[»]
    3S16X-ray3.24C1-318[»]
    3S17X-ray3.20C1-318[»]
    3S1MX-ray3.13C1-318[»]
    3S1NX-ray3.10C1-318[»]
    3S1QX-ray3.30C1-318[»]
    3S1RX-ray3.20C1-318[»]
    3S2DX-ray3.20C1-318[»]
    3S2HX-ray3.30C1-318[»]
    4A3BX-ray3.50C1-318[»]
    4A3CX-ray3.50C1-318[»]
    4A3DX-ray3.40C1-318[»]
    4A3EX-ray3.40C1-318[»]
    4A3FX-ray3.50C1-318[»]
    4A3GX-ray3.50C1-318[»]
    4A3IX-ray3.80C1-318[»]
    4A3JX-ray3.70C1-318[»]
    4A3KX-ray3.50C1-318[»]
    4A3LX-ray3.50C1-318[»]
    4A3MX-ray3.90C1-318[»]
    4A93X-ray3.40C1-318[»]
    4BBRX-ray3.40C1-318[»]
    4BBSX-ray3.60C1-318[»]
    4BXXX-ray3.28C1-318[»]
    4BXZX-ray4.80C1-318[»]
    4BY1X-ray3.60C1-318[»]
    4BY7X-ray3.15C1-318[»]
    ProteinModelPortaliP16370.
    SMRiP16370. Positions 3-268.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16370.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0202.
    GeneTreeiENSGT00640000091532.
    HOGENOMiHOG000230844.
    KOiK03011.
    OMAiHTDYWYE.
    OrthoDBiEOG776T14.

    Family and domain databases

    Gene3Di2.170.120.12. 1 hit.
    InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
    IPR011262. DNA-dir_RNA_pol_insert.
    IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
    IPR009025. RBP11-like_dimer.
    [Graphical view]
    PfamiPF01000. RNA_pol_A_bac. 1 hit.
    PF01193. RNA_pol_L. 1 hit.
    [Graphical view]
    SMARTiSM00662. RPOLD. 1 hit.
    [Graphical view]
    SUPFAMiSSF55257. SSF55257. 1 hit.
    SSF56553. SSF56553. 1 hit.
    PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16370-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE    50
    VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCED HCDKCSVVLT 100
    LQAFGESEST TNVYSKDLVI VSNLMGRNIG HPIIQDKEGN GVLICKLRKG 150
    QELKLTCVAK KGIAKEHAKW GPAAAIEFEY DPWNKLKHTD YWYEQDSAKE 200
    WPQSKNCEYE DPPNEGDPFD YKAQADTFYM NVESVGSIPV DQVVVRGIDT 250
    LQKKVASILL ALTQMDQDKV NFASGDNNTA SNMLGSNEDV MMTGAEQDPY 300
    SNASQMGNTG SGGYDNAW 318
    Length:318
    Mass (Da):35,298
    Last modified:February 1, 1995 - v2
    Checksum:i8E1D1E6BB7B51D89
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti175 – 1751A → G in AAA34889. (PubMed:2685562)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti30 – 301A → D in mutant RPB3-1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46881 Genomic DNA. Translation: CAA86971.1.
    M27496 Genomic DNA. Translation: AAA34889.1.
    BK006942 Genomic DNA. Translation: DAA08524.1.
    PIRiS49961.
    RefSeqiNP_012243.3. NM_001179371.3.

    Genome annotation databases

    EnsemblFungiiYIL021W; YIL021W; YIL021W.
    GeneIDi854791.
    KEGGisce:YIL021W.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46881 Genomic DNA. Translation: CAA86971.1 .
    M27496 Genomic DNA. Translation: AAA34889.1 .
    BK006942 Genomic DNA. Translation: DAA08524.1 .
    PIRi S49961.
    RefSeqi NP_012243.3. NM_001179371.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1I3Q X-ray 3.10 C 1-318 [» ]
    1I50 X-ray 2.80 C 1-318 [» ]
    1I6H X-ray 3.30 C 1-318 [» ]
    1K83 X-ray 2.80 C 1-318 [» ]
    1NIK X-ray 4.10 C 1-318 [» ]
    1NT9 X-ray 4.20 C 1-318 [» ]
    1PQV X-ray 3.80 C 1-318 [» ]
    1R5U X-ray 4.50 C 1-318 [» ]
    1R9S X-ray 4.25 C 1-318 [» ]
    1R9T X-ray 3.50 C 1-318 [» ]
    1SFO X-ray 3.61 C 1-318 [» ]
    1TWA X-ray 3.20 C 1-318 [» ]
    1TWC X-ray 3.00 C 1-318 [» ]
    1TWF X-ray 2.30 C 1-318 [» ]
    1TWG X-ray 3.30 C 1-318 [» ]
    1TWH X-ray 3.40 C 1-318 [» ]
    1WCM X-ray 3.80 C 1-318 [» ]
    1Y1V X-ray 3.80 C 1-318 [» ]
    1Y1W X-ray 4.00 C 1-318 [» ]
    1Y1Y X-ray 4.00 C 1-318 [» ]
    1Y77 X-ray 4.50 C 1-318 [» ]
    2B63 X-ray 3.80 C 1-318 [» ]
    2B8K X-ray 4.15 C 1-318 [» ]
    2E2H X-ray 3.95 C 1-318 [» ]
    2E2I X-ray 3.41 C 1-318 [» ]
    2E2J X-ray 3.50 C 1-318 [» ]
    2JA5 X-ray 3.80 C 1-318 [» ]
    2JA6 X-ray 4.00 C 1-318 [» ]
    2JA7 X-ray 3.80 C/O 1-318 [» ]
    2JA8 X-ray 3.80 C 1-318 [» ]
    2NVQ X-ray 2.90 C 1-318 [» ]
    2NVT X-ray 3.36 C 1-318 [» ]
    2NVX X-ray 3.60 C 1-318 [» ]
    2NVY X-ray 3.40 C 1-318 [» ]
    2NVZ X-ray 4.30 C 1-318 [» ]
    2R7Z X-ray 3.80 C 1-318 [» ]
    2R92 X-ray 3.80 C 1-318 [» ]
    2R93 X-ray 4.00 C 1-318 [» ]
    2VUM X-ray 3.40 C 1-318 [» ]
    2YU9 X-ray 3.40 C 1-318 [» ]
    3CQZ X-ray 2.80 C 1-318 [» ]
    3FKI X-ray 3.88 C 1-318 [» ]
    3GTG X-ray 3.78 C 1-318 [» ]
    3GTJ X-ray 3.42 C 1-318 [» ]
    3GTK X-ray 3.80 C 1-318 [» ]
    3GTL X-ray 3.38 C 1-318 [» ]
    3GTM X-ray 3.80 C 1-318 [» ]
    3GTO X-ray 4.00 C 1-318 [» ]
    3GTP X-ray 3.90 C 1-318 [» ]
    3GTQ X-ray 3.80 C 1-318 [» ]
    3H3V X-ray 4.00 D 1-318 [» ]
    3HOU X-ray 3.20 C/O 1-318 [» ]
    3HOV X-ray 3.50 C 1-318 [» ]
    3HOW X-ray 3.60 C 2-318 [» ]
    3HOX X-ray 3.65 C 2-318 [» ]
    3HOY X-ray 3.40 C 2-318 [» ]
    3HOZ X-ray 3.65 C 2-318 [» ]
    3I4M X-ray 3.70 C 1-318 [» ]
    3I4N X-ray 3.90 C 1-318 [» ]
    3J0K electron microscopy 36.00 C 1-268 [» ]
    3J1N electron microscopy 16.00 C 1-268 [» ]
    3K1F X-ray 4.30 C 1-318 [» ]
    3K7A X-ray 3.80 C 1-318 [» ]
    3M3Y X-ray 3.18 C 1-318 [» ]
    3M4O X-ray 3.57 C 1-318 [» ]
    3PO2 X-ray 3.30 C 1-318 [» ]
    3PO3 X-ray 3.30 C 1-318 [» ]
    3QT1 X-ray 4.30 C 1-318 [» ]
    3RZD X-ray 3.30 C 1-318 [» ]
    3RZO X-ray 3.00 C 1-318 [» ]
    3S14 X-ray 2.85 C 1-318 [» ]
    3S15 X-ray 3.30 C 1-318 [» ]
    3S16 X-ray 3.24 C 1-318 [» ]
    3S17 X-ray 3.20 C 1-318 [» ]
    3S1M X-ray 3.13 C 1-318 [» ]
    3S1N X-ray 3.10 C 1-318 [» ]
    3S1Q X-ray 3.30 C 1-318 [» ]
    3S1R X-ray 3.20 C 1-318 [» ]
    3S2D X-ray 3.20 C 1-318 [» ]
    3S2H X-ray 3.30 C 1-318 [» ]
    4A3B X-ray 3.50 C 1-318 [» ]
    4A3C X-ray 3.50 C 1-318 [» ]
    4A3D X-ray 3.40 C 1-318 [» ]
    4A3E X-ray 3.40 C 1-318 [» ]
    4A3F X-ray 3.50 C 1-318 [» ]
    4A3G X-ray 3.50 C 1-318 [» ]
    4A3I X-ray 3.80 C 1-318 [» ]
    4A3J X-ray 3.70 C 1-318 [» ]
    4A3K X-ray 3.50 C 1-318 [» ]
    4A3L X-ray 3.50 C 1-318 [» ]
    4A3M X-ray 3.90 C 1-318 [» ]
    4A93 X-ray 3.40 C 1-318 [» ]
    4BBR X-ray 3.40 C 1-318 [» ]
    4BBS X-ray 3.60 C 1-318 [» ]
    4BXX X-ray 3.28 C 1-318 [» ]
    4BXZ X-ray 4.80 C 1-318 [» ]
    4BY1 X-ray 3.60 C 1-318 [» ]
    4BY7 X-ray 3.15 C 1-318 [» ]
    ProteinModelPortali P16370.
    SMRi P16370. Positions 3-268.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34967. 194 interactions.
    DIPi DIP-837N.
    IntActi P16370. 32 interactions.
    MINTi MINT-613688.
    STRINGi 4932.YIL021W.

    Proteomic databases

    MaxQBi P16370.
    PaxDbi P16370.
    PeptideAtlasi P16370.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL021W ; YIL021W ; YIL021W .
    GeneIDi 854791.
    KEGGi sce:YIL021W.

    Organism-specific databases

    CYGDi YIL021w.
    SGDi S000001283. RPB3.

    Phylogenomic databases

    eggNOGi COG0202.
    GeneTreei ENSGT00640000091532.
    HOGENOMi HOG000230844.
    KOi K03011.
    OMAi HTDYWYE.
    OrthoDBi EOG776T14.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31296-MONOMER.
    Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

    Miscellaneous databases

    EvolutionaryTracei P16370.
    NextBioi 977585.
    PROi P16370.

    Gene expression databases

    Genevestigatori P16370.

    Family and domain databases

    Gene3Di 2.170.120.12. 1 hit.
    InterProi IPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
    IPR011262. DNA-dir_RNA_pol_insert.
    IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
    IPR009025. RBP11-like_dimer.
    [Graphical view ]
    Pfami PF01000. RNA_pol_A_bac. 1 hit.
    PF01193. RNA_pol_L. 1 hit.
    [Graphical view ]
    SMARTi SM00662. RPOLD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55257. SSF55257. 1 hit.
    SSF56553. SSF56553. 1 hit.
    PROSITEi PS00446. RNA_POL_D_30KD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus."
      Kolodziej P., Young R.A.
      Mol. Cell. Biol. 9:5387-5394(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 206-223.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "cis- and trans-Acting determinants of transcription termination by yeast RNA polymerase II."
      Steinmetz E.J., Ng S.B., Cloute J.P., Brow D.A.
      Mol. Cell. Biol. 26:2688-2696(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LYS-9.
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    7. "RNA polymerase II/TFIIF structure and conserved organization of the initiation complex."
      Chung W.H., Craighead J.L., Chang W.H., Ezeokonkwo C., Bareket-Samish A., Kornberg R.D., Asturias F.J.
      Mol. Cell 12:1003-1013(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF THE RNA POL II/TFIIF COMPLEX.
    8. "Structural basis of transcription: RNA polymerase II at 2.8 A resolution."
      Cramer P., Bushnell D.A., Kornberg R.D.
      Science 292:1863-1876(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    9. "Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution."
      Gnatt A.L., Cramer P., Fu J., Bushnell D.A., Kornberg R.D.
      Science 292:1876-1882(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    10. "Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution."
      Bushnell D.A., Cramer P., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:1218-1222(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE RNA POL II CORE COMPLEX IN COMPLEX WITH ALPHA-AMANITIN.
    11. "Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage."
      Kettenberger H., Armache K.J., Cramer P.
      Cell 114:347-357(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH DST1.
    12. "Architecture of initiation-competent 12-subunit RNA polymerase II."
      Armache K.J., Kettenberger H., Cramer P.
      Proc. Natl. Acad. Sci. U.S.A. 100:6964-6968(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS) OF THE RNA POL II COMPLEX.
    13. "Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription."
      Bushnell D.A., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 100:6969-6973(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    14. "Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center."
      Westover K.D., Bushnell D.A., Kornberg R.D.
      Cell 119:481-489(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    15. "Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS."
      Kettenberger H., Armache K.J., Cramer P.
      Mol. Cell 16:955-965(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS).
    16. "Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms."
      Bushnell D.A., Westover K.D., Davis R.E., Kornberg R.D.
      Science 303:983-988(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    17. "Structures of complete RNA polymerase II and its subcomplex, Rpb4/7."
      Armache K.J., Mitterweger S., Meinhart A., Cramer P.
      J. Biol. Chem. 280:7131-7134(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX.
    18. "Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs."
      Kettenberger H., Eisenfuhr A., Brueckner F., Theis M., Famulok M., Cramer P.
      Nat. Struct. Mol. Biol. 13:44-48(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH INHIBITING NON-CODING RNA.
    19. "Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model."
      Meyer P.A., Ye P., Zhang M., Suh M.H., Fu J.
      Structure 14:973-982(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.15 ANGSTROMS) OF THE RNA POL II COMPLEX.

    Entry informationi

    Entry nameiRPB3_YEAST
    AccessioniPrimary (citable) accession number: P16370
    Secondary accession number(s): D6VVQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3