Metalloproteinase inhibitor 2 precursor

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Reviewed, UniProtKB/Swiss-Prot P16368 (TIMP2_BOVIN)

Last modified September 1, 2009. Version 89. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Metalloproteinase inhibitor 2
Alternative name(s):
    Tissue inhibitor of metalloproteinases 2
      Short name=TIMP-2
    Collagenase inhibitor

Gene names

Name:

TIMP2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them.
Subcellular location	Secreted.
Post-translational modification	The activity of TIMP2 is dependent on the presence of disulfide bonds.
Sequence similarities	Belongs to the protease inhibitor I35 (TIMP) family. [View classification] Contains 1 NTR domain.

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Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
MMP14	P50281	1	EBI-1027047,EBI-992788	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Ref.4 Ref.5

Chain

27 – 220

194

Metalloproteinase inhibitor 2

PRO_0000034331

Regions

Domain

27 – 152

126

NTR

Amino acid modifications

Disulfide bond

27 ↔ 98

By similarity

Disulfide bond

29 ↔ 127

By similarity

Disulfide bond

39 ↔ 152

By similarity

Disulfide bond

154 ↔ 201

By similarity

Disulfide bond

159 ↔ 164

By similarity

Disulfide bond

172 ↔ 193

By similarity

Experimental info

Sequence conflict

L → M in AAI02711. Ref.2

Sequence conflict

D → C AA sequence Ref.4

Sequence conflict

D → E AA sequence Ref.4

Sequence conflict

R → S AA sequence Ref.4

Sequence conflict

F → L in AAI02711. Ref.2

Sequence conflict

94 – 95

AA → SS in AAI02711. Ref.2

Secondary structure

220

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P16368-1 [UniParc].

Last modified May 1, 1991. Version 2.
Checksum: 9A5438737110E7B7
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FASTA

220

24,355

        10         20         30         40         50         60 
MGAAARSLPL AFCLLLLGTL LPRADACSCS PVHPQQAFCN ADIVIRAKAV NKKEVDSGND 

        70         80         90        100        110        120 
IYGNPIKRIQ YEIKQIKMFK GPDQDIEFIY TAPAAAVCGV SLDIGGKKEY LIAGKAEGNG 

       130        140        150        160        170        180 
NMHITLCDFI VPWDTLSATQ KKSLNHRYQM GCECKITRCP MIPCYISSPD ECLWMDWVTE 

       190        200        210        220 
KNINGHQAKF FACIKRSDGS CAWYRGAAPP KQEFLDIEDP

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of a metalloproteinase inhibitor related to tissue inhibitor of metalloproteinases." Boone T.C., Johnson M.J., de Clerck Y.A., Langley K.E. Proc. Natl. Acad. Sci. U.S.A. 87:2800-2804(1990) [PubMed: 2157214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[3]	"Involvement of fibroblasts and muscle cells in the expression of an extracellular proteolytic cascade in bovine skeletal muscle." Balcerzak D., Querengesser L., Dixon W.T., Baracos V.E. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-218. Tissue: Skeletal muscle.
[4]	"Purification and partial amino acid sequence of a bovine cartilage-derived collagenase inhibitor." Murray J.B., Allison K., Sudhalter J., Langer R. J. Biol. Chem. 261:4154-4159(1986) [PubMed: 3005321] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-71. Tissue: Cartilage.
[5]	"Purification and characterization of two related but distinct metalloproteinase inhibitors secreted by bovine aortic endothelial cells." de Clerck Y.A., Yean T.D., Ratzkin B.J., Lu H.S., Langley K.E. J. Biol. Chem. 264:17445-17453(1989) [PubMed: 2551903] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-71.
[6]	"Characterization of the functional domain of tissue inhibitor of metalloproteinases-2 (TIMP-2)." De Clerck Y.A., Yean T.D., Lee Y., Tomich J.M., Langley K.E. Biochem. J. 289:65-69(1993) [PubMed: 8424773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-71.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M32303 mRNA. Translation: AAA30636.1.
BC102710 mRNA. Translation: AAI02711.1.
AF144764 mRNA. Translation: AAD30304.1.

IPI

IPI00710784.

PIR

A35996.

RefSeq

NP_776897.2.

UniGene

Bt.52974

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BQQ	X-ray	2.75	T	27-210	[»]
1BUV	X-ray	2.75	T	27-210	[»]
2E2D	X-ray	2.00	C	28-206	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P16368. 1 interaction.

STRING

P16368.

Protein family/group databases

MEROPS

I35.002.

Genome annotation databases

Ensembl

ENSBTAT00000014476; ENSBTAP00000014476; ENSBTAG00000010899; Bos taurus. [Genome view]

GeneID

282093.

KEGG

bta:282093.

Organism-specific databases

CTD

282093.

Phylogenomic databases

HOVERGEN

P16368.

Family and domain databases

InterPro

IPR001134. Netrin_domain.
IPR001820. Prot_inh_TIMP.
IPR015613. TIMP2.
[Graphical view]

PANTHER

PTHR11844. Prot_inh_TIMP. 1 hit.
PTHR11844:SF7. TIMP2. 1 hit.

Pfam

PF00965. TIMP. 1 hit.
[Graphical view]

SMART

SM00206. NTR. 1 hit.
[Graphical view]

PROSITE

PS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

TIMP2_BOVIN

Accession

Primary (citable) accession number: P16368
Secondary accession number(s): Q3SZU3, Q9TVB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	May 1, 1991
Last modified:	September 1, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents