Reviewed, UniProtKB/Swiss-Prot P16355 (RPA1_TRYBB)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA1
      Short name=TbRPA1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I largest subunit

Gene names

Name:

TRP11

Organism

Trypanosoma brucei brucei

Taxonomic identifier

5702 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	1744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Post-translational modification	Phosphorylated. Ref.3
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1744	1744	DNA-directed RNA polymerase I subunit RPA1		PRO_0000073928
Regions
	Zinc finger	53 – 72	20	C2H2-type; degenerate
	Region	953 – 965	13	Bridging helix By similarity
Sites
	Metal binding	597	1	Magnesium; catalytic By similarity
	Metal binding	599	1	Magnesium; catalytic By similarity
	Metal binding	601	1	Magnesium; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16355-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 5D1D2C6C7B89229F	FASTA	1,744	191,769
	10 20 30 40 50 60 MSGIAFVEVR TRAGQEDRSA PWRPVVRNGE NHATFYDTRM GNFDANPFPP QTCQTCAASL 70 80 90 100 110 120 TGKYGNERCH GHFGFVGMPR IRPGSAHSDS DRLVVLNPHL AMDADRLFRA KCFFCHKFRA 130 140 150 160 170 180 PTFDVERFRQ ALVLADHGLP GDALHLLDTV PTAKGHDAML NHRRMANEEI VNDVSILQSY 190 200 210 220 230 240 VDRILRQRAS GCSEEDAKAR VTMAQKGTVD VRNDICNMAI SHLRSFSGPC SHCTAISPTF 250 260 270 280 290 300 LKRGGIIFFL FRKSNLVTNI AKGFLTQQEV SEWEAVNRLH GRTGTYFDGR QMLFHMKNLF 310 320 330 340 350 360 AKEQAILGLL YPNLGEPSVF TKTNKVVPAS ERYKLFFLDR ILVPPLPLRL SSGVRVNDNG 370 380 390 400 410 420 LIIPDEQTRA LSDILGFVEQ IECFHTLSAN STNGRSFITD AQRAVNESNL RNLQQKVDEF 430 440 450 460 470 480 YAEIVNSFAK KEGLFRMNMM GKRVNQACRS VISPDPFVEP NEVLLPRPLA RALSFPEQVT 490 500 510 520 530 540 CFAPARMNLL KHCVVNGPRK YPGATHIELR HANGEIRSVD LNVPEQTRRQ HAARFFAMAQ 550 560 570 580 590 600 SGVTLIVYRH ILNGDRVIFN RQPTLHKPSM MGYRVKVLSG SKTIRFHYVN GNSFNADFDG 610 620 630 640 650 660 DEMNVHVPQS IETRAEVETL MDANINYLVP TSGRPIRGLI QDHVAAGVLV TLRDKFFDHS 670 680 690 700 710 720 TFVQLVYNGV GPYIQENVGI TLAELIPIPA ILMPRPMWTG KQLISVMVRF SSGLSAASDC 730 740 750 760 770 780 GREIEGGITL KGTSQIQPSA FDRIPAGSCD AVRAKSGAVV DSTVMFANSE LITGFMCKKQ 790 800 810 820 830 840 LGASNMSAPH HVYELYGPHR TGQLFAAFGR VLLLALRKEG LSLAMDDMFL VDEERRCDLL 850 860 870 880 890 900 RKLDDIALDV PDEEATAAPM IADYATKIQQ EFVPQRMLVP FPKNHLLLMT ISGAKGSNLN 910 920 930 940 950 960 ATQMSLQLGQ QLFDGLRVKR MNSSKTLPSF FTNEKRARSF GFAMGSFASG IRPAEYTIHA 970 980 990 1000 1010 1020 MAGRDGLIDT AVKTSRSGHL QRCLIKGLES LVVHWDRTVR DSNGSVIQFM YGGDGLDPCK 1030 1040 1050 1060 1070 1080 ASTLTAWEMM KDNVVDVSKR FGGDASESVA GAEDGAAAGL KEMRNEDGKP TTEAVQNAHM 1090 1100 1110 1120 1130 1140 EQQLSTYPLP ASLDKSLSEY LCKKADFPLF RKVSTLARWD AKQQLKERLQ QRRQKWVGAF 1150 1160 1170 1180 1190 1200 EKTLADITAR RRLWALCEPG EPVGLLAAQA AGEPSTQMTL NTFHTAGSTV SHVTEGIPRL 1210 1220 1230 1240 1250 1260 RELLIYASVN KAAVVVPVTN ATEEDEKVIA KMLRAGVAAK LTDCLAKVTD GAGGQSASSS 1270 1280 1290 1300 1310 1320 MQRNLNTGFG KGYHYHVARG RTGMVITVSF LFSRSCLEEL RKRMCMSPSE HRQSFTEALK 1330 1340 1350 1360 1370 1380 NVVRLIMRSL SAVPREKESG DGSGNTGGMK GGSGRADRKR KRSGPDDGGG PLGGTFGDEI 1390 1400 1410 1420 1430 1440 MRIEEGTDSD DGMSERSSIG GGRAGSEVSS LHSDGTDTRG IAGSDTGGPQ RRRGSVESGR 1450 1460 1470 1480 1490 1500 GDDASDSEAA DPDLYARRSG SPARDAEDGG EMQDRDGTDW GGTSMQGVVG YDNFPEIHMS 1510 1520 1530 1540 1550 1560 FTKSNFGAVI APLSTAAAAR DGVVQLHEDF FIVNAVLRTA SDVIAVIPDV VDNALEAQRM 1570 1580 1590 1600 1610 1620 PSWLPQFGSL TFTRLKDKGS GQLVFQGPGS TMRNVMSFLS LFTVGIKSIK LHQACSTDIR 1630 1640 1650 1660 1670 1680 DMGTYFGIES GYAALYDELN KLFNRYNVDP RHLSLIADTS THRGRWENFN FTGVISTSAS 1690 1700 1710 1720 1730 1740 PLFQMTFASS KRWLHRAVSR GMSDDLESFS SAIMVGERPR VGTASVRLST DTAILRDVLE RNFA

References

[1]	"Complete sequence of the gene encoding the largest subunit of RNA polymerase I of Trypanosoma brucei." Jess W., Hammer A., Cornelissen A.W.C.A. FEBS Lett. 249:123-128(1989) [PubMed: 2542092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 427.
[2]	Erratum Jess W., Hammer A., Cornelissen A.W.C.A. FEBS Lett. 258:180-180(1989) Cited for: SEQUENCE REVISION.
[3]	"Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei." Walgraffe D., Devaux S., Lecordier L., Dierick J.F., Dieu M., Van den Abbeele J., Pays E., Vanhamme L. Mol. Biochem. Parasitol. 139:249-260(2005) [PubMed: 15664659] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX, PHOSPHORYLATION.
[4]	"Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei." Nguyen T.N., Schimanski B., Zahn A., Klumpp B., Gunzl A. Mol. Biochem. Parasitol. 149:27-37(2006) [PubMed: 16730080] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14399 Genomic DNA. Translation: CAA32572.1.
3D structure databases
HSSP	HSSP built from PDB template 1I6H based on UniProtKB P04050.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.6. 74165.
Family and domain databases
InterPro	IPR015699. DNA-dir_RNA_pol1_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit.
PANTHER	PTHR19376:SF11. RNA_polyI. 1 hit.
Pfam	PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA1_TRYBB

Accession

Primary (citable) accession number: P16355

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)