P16348 (API11_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Aspartic protease inhibitor 11 Alternative name(s): Cathepsin D inhibitor PDI Allergen=Sola t 2
Organism	Solanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of cathepsin D (aspartic protease) and trypsin (serine protease). May protect the plant by inhibiting proteases of invading organisms.
Subcellular location	Vacuole By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the protease inhibitor I3 (leguminous Kunitz-type inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Vacuole
Disease	Allergen
Molecular function	Aspartic protease inhibitor Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of endopeptidase activity Inferred from electronic annotation. Source: GOC
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Aspartic protease inhibitor 11		PRO_0000083313
Sites
	Site	67 – 68	2	Reactive bond for trypsin
	Site	111 – 112	2	Reactive bond for chymotrypsin By similarity
Amino acid modifications
	Glycosylation	19	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	48 ↔ 93		By similarity
	Disulfide bond	142 ↔ 159		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16348 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 4E8A49113EF6F10D	FASTA	188	20,590
	10 20 30 40 50 60 ESPLPKPVLD TNGKELNPNS SYRIISIGRG ALGGDVYLGK SPNSDAPCPD GVFRYNSDVG 70 80 90 100 110 120 PSGTPVRFIP LSGGIFEDQL LNIQFNIATV KLCVSYTIWK VGNLNAYFRT MLLETGGTIG 130 140 150 160 170 180 QADSSYFKIV KLSNFGYNLL YCPITPPFLC PFCRDDNFCA KVGVVIQNGK RRLALVNENP LDVLFQEV

References

[1]	"Primary structure of cathepsin D inhibitor from potatoes and its structure relationship to soybean trypsin inhibitor family." Mares M., Meloun B., Pavlik M., Kostka V., Baudys M. FEBS Lett. 251:94-98(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	XKPODC. S05025.
3D structure databases
ProteinModelPortal	P16348.
ModBase	Search...
Protein family/group databases
Allergome	3491. Sola t 2.0101. 640. Sola t 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011065. Kunitz_inhibitor_ST1-like. IPR002160. Prot_inh_Kunz-lg. [Graphical view]
Pfam	PF00197. Kunitz_legume. 1 hit. [Graphical view]
PRINTS	PR00291. KUNITZINHBTR.
SMART	SM00452. STI. 1 hit. [Graphical view]
SUPFAM	SSF50386. Kunitz_like. 1 hit.
PROSITE	PS00283. SOYBEAN_KUNITZ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

API11_SOLTU

Accession

Primary (citable) accession number: P16348

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program