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Protein

Endogenous alpha-amylase/subtilisin inhibitor

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of endogenous alpha-amylase (wheat also produces an exogenous inhibitor which inactivates alpha-amylase from animal and insect origin). This inhibitor can also inhibit subtilisin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei66 – 67Reactive bondBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionAlpha-amylase inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI03.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Endogenous alpha-amylase/subtilisin inhibitor
Alternative name(s):
WASI
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unplaced

Pathology & Biotechi

Protein family/group databases

Allergomei9593. Tri a aA_SI.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833221 – 180Endogenous alpha-amylase/subtilisin inhibitorAdd BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 89
Disulfide bondi143 ↔ 147

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP16347.

Structurei

3D structure databases

ProteinModelPortaliP16347.
SMRiP16347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd00178. STI. 1 hit.
InterProiView protein in InterPro
IPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
PfamiView protein in Pfam
PF00197. Kunitz_legume. 1 hit.
PRINTSiPR00291. KUNITZINHBTR.
SMARTiView protein in SMART
SM00452. STI. 1 hit.
SUPFAMiSSF50386. SSF50386. 1 hit.
PROSITEiView protein in PROSITE
PS00283. SOYBEAN_KUNITZ. 1 hit.

Sequencei

Sequence statusi: Complete.

P16347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DPPPVHDTDG NELRADANYY VLPANRAHGG GLTMAPGHGR RCPLFVSQEA
60 70 80 90 100
DGQRDGLPVR IAPHGGAPSD KIIRLSTDVR ISFRAYTTCV QSTEWHIDSE
110 120 130 140 150
LVSGRRHVIT GPVRDPSPSG RENAFRIEKY SGAEVHEYKL MACGDSCQDL
160 170 180
GVFRDLKGGA WFLGATEPYH VVVFKKAPPA
Length:180
Mass (Da):19,633
Last modified:August 1, 1990 - v1
Checksum:iDFF9F2A6DD47B561
GO

Sequence databases

PIRiS38955.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIAAS_WHEAT
AccessioniPrimary (citable) accession number: P16347
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 10, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families