P16340 (PUR2_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trifunctional purine biosynthetic protein adenosine-3

Including the following 3 domains:

1. Phosphoribosylamine--glycine ligase
   EC=6.3.4.13
   Alternative name(s):
   Glycinamide ribonucleotide synthetase
   Short name=GARS
   Phosphoribosylglycinamide synthetase
2. Phosphoribosylformylglycinamidine cyclo-ligase
   EC=6.3.3.1
   Alternative name(s):
   AIR synthase
   Short name=AIRS
   Phosphoribosyl-aminoimidazole synthetase
3. Phosphoribosylglycinamide formyltransferase
   EC=2.1.2.2
   Alternative name(s):
   5'-phosphoribosylglycinamide transformylase
   GAR transformylase
   Short name=GART

Gene names

Name:	ade3
Synonyms:	gart
ORF Names:	GA16345

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	1364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similarities	In the N-terminal section; belongs to the GARS family. In the central section; belongs to the AIR synthase family. In the C-terminal section; belongs to the GART family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway methylation Inferred from electronic annotation. Source: GOC purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P16340-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P16340-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1364: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1364	1364	Trifunctional purine biosynthetic protein adenosine-3		PRO_0000074935
Regions
	Domain	114 – 321	208	ATP-grasp
	Nucleotide binding	140 – 202	63	ATP By similarity
	Region	435 – 1154	720	AIRS
	Region	1155 – 1364	210	GART
	Region	1166 – 1168	3	5'-phosphoribosylglycinamide binding By similarity
	Region	1246 – 1249	4	10-formyltetrahydrofolate binding By similarity
	Region	1297 – 1301	5	10-formyltetrahydrofolate binding By similarity
	Region	1327 – 1330	4	5'-phosphoribosylglycinamide binding By similarity
Sites
	Active site	1265	1	Proton donor By similarity
	Metal binding	291	1	Manganese By similarity
	Metal binding	293	1	Manganese By similarity
	Binding site	1221	1	10-formyltetrahydrofolate By similarity
	Binding site	1263	1	10-formyltetrahydrofolate By similarity
	Site	1301	1	Raises pKa of active site His By similarity
Natural variations
	Alternative sequence	434	1	I → M in isoform Short.		VSP_005514
	Alternative sequence	435 – 1364	930	Missing in isoform Short.		VSP_005515
Experimental info
	Sequence conflict	327	1	L → M in AAX12966. Ref.3
	Sequence conflict	425	1	T → M in AAX12966. Ref.3
	Sequence conflict	805	1	T → S in CAA29611. Ref.1
	Sequence conflict	956	1	G → A in CAA29611. Ref.1
	Sequence conflict	1145	1	A → T in CAA29611. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 4BDF1C7BD435E642	FASTA	1,364	145,663
	10 20 30 40 50 60 MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA KVLDPKDFEA 70 80 90 100 110 120 IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG KQGAQIEADK KWAKDFMLRH 130 140 150 160 170 180 GIPTARYESF TDTNKAKAFI RSAPYQALVV KAAGLAAGKG VVVAANVDEA CQAVDEILGD 190 200 210 220 230 240 LKYGQAGATL VVEELLEGEE ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 250 260 270 280 290 300 PCPLISQPAL ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 310 320 330 340 350 360 TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET STKGCLITGL 370 380 390 400 410 420 PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL KEAAAKATKL AGTITFAGTG 430 440 450 460 470 480 AQYRTDIAQK AFKIAIATAP GLSYKDSGVD IDAGDALVQR IKPLSRGTQR PGVLGGLGGF 490 500 510 520 530 540 GGLFRLKDLS YKEPVIAEAT QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA 550 560 570 580 590 600 FLDYIACGKL HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV 610 620 630 640 650 660 EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP VGGGKHGLSL 670 680 690 700 710 720 AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ RLLPEGFEVT LDFGAVPVPD 730 740 750 760 770 780 VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL PQSSLLWRTA LPGAKVLGVL NRQAKASGGA 790 800 810 820 830 840 PRVKVRNFVE QLQKLAAPFG GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP 850 860 870 880 890 900 NHYVDPILIL GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG 910 920 930 940 950 960 KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG IAERSGILPR 970 980 990 1000 1010 1020 LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR APFSEKTLGE ELLVPTKIYV 1030 1040 1050 1060 1070 1080 KALSALLSRP NHGIKALAHI TGGGLSENIP RVLRKELAVR LDANKYPLPP VFAWLAAAGN 1090 1100 1110 1120 1130 1140 ISSTELQRTY NCGLGLVLVV GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF 1150 1160 1170 1180 1190 1200 EASLARTQRM LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL 1210 1220 1230 1240 1250 1260 ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS VPFVREWRGR 1270 1280 1290 1300 1310 1320 LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV DTGAIIVQAA VPILPGDDEE 1330 1340 1350 1360 TLTQRIHYAE HWAFPRALAL LASGALRRVS EVKKEAPKDI KDSQ
	Isoform Short [UniParc]. Checksum: B0067502F4A69D52	FASTA	434	46,252
	10 20 30 40 50 60 MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA KVLDPKDFEA 70 80 90 100 110 120 IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG KQGAQIEADK KWAKDFMLRH 130 140 150 160 170 180 GIPTARYESF TDTNKAKAFI RSAPYQALVV KAAGLAAGKG VVVAANVDEA CQAVDEILGD 190 200 210 220 230 240 LKYGQAGATL VVEELLEGEE ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 250 260 270 280 290 300 PCPLISQPAL ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 310 320 330 340 350 360 TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET STKGCLITGL 370 380 390 400 410 420 PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL KEAAAKATKL AGTITFAGTG 430 AQYRTDIAQK AFKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conserved arrangement of nested genes at the Drosophila Gart locus." Henikoff S., Eghtedarzadeh M.K. Genetics 117:711-725(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: EST10.
[2]	"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.
[3]	"Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda." Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B. Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-565.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06285 Genomic DNA. Translation: CAA29611.1. CH379058 Genomic DNA. Translation: EAL34461.2. AY754390 Genomic DNA. Translation: AAX12966.1.
PIR	AJFFPP. S01204.
RefSeq	XP_001357392.2. XM_001357356.2.
3D structure databases
ProteinModelPortal	P16340.
ModBase	Search...
Protein-protein interaction databases
STRING	7237.FBpp0279413.
Proteomic databases
PRIDE	P16340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0280975; FBpp0279413; FBgn0012717.
GeneID	4817813.
KEGG	dpo:Dpse_GA25439.
Organism-specific databases
FlyBase	FBgn0012717. Dpse\ade3.
Phylogenomic databases
eggNOG	COG0151.
InParanoid	P16340.
KO	K11787.
OrthoDB	EOG4THT80.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125. UPA00074; UER00126. UPA00074; UER00129.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.170. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit. 3.90.650.10. 2 hits.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR004607. PurN_trans. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. PF00551. Formyl_trans_N. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF53328. formyl_transf. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF55326. PurM_N-like. 2 hits. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit. TIGR00878. purM. 2 hits. TIGR00639. PurN. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_DROPS

Accession

Primary (citable) accession number: P16340
Secondary accession number(s): Q29P31, Q56RW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	June 27, 2006
Last modified:	May 29, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project