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P16340

- PUR2_DROPS

UniProt

P16340 - PUR2_DROPS

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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

ade3

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi291 – 2911ManganeseBy similarity
Metal bindingi293 – 2931ManganeseBy similarity
Binding sitei1221 – 1221110-formyltetrahydrofolateBy similarity
Binding sitei1263 – 1263110-formyltetrahydrofolateBy similarity
Active sitei1265 – 12651Proton donorBy similarity
Sitei1301 – 13011Raises pKa of active site HisBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 20263ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
  6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:ade3
Synonyms:gart
ORF Names:GA16345
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0012717. Dpse\ade3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13641364Trifunctional purine biosynthetic protein adenosine-3PRO_0000074935Add
BLAST

Proteomic databases

PRIDEiP16340.

Interactioni

Protein-protein interaction databases

STRINGi7237.FBpp0279413.

Structurei

3D structure databases

ProteinModelPortaliP16340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 321208ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni435 – 1154720AIRSAdd
BLAST
Regioni1155 – 1364210GARTAdd
BLAST
Regioni1166 – 116835'-phosphoribosylglycinamide bindingBy similarity
Regioni1246 – 1249410-formyltetrahydrofolate bindingBy similarity
Regioni1297 – 1301510-formyltetrahydrofolate bindingBy similarity
Regioni1327 – 133045'-phosphoribosylglycinamide bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG0151.
InParanoidiP16340.
KOiK11787.
OrthoDBiEOG7RBZ7H.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P16340-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA
60 70 80 90 100
KVLDPKDFEA IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG
110 120 130 140 150
KQGAQIEADK KWAKDFMLRH GIPTARYESF TDTNKAKAFI RSAPYQALVV
160 170 180 190 200
KAAGLAAGKG VVVAANVDEA CQAVDEILGD LKYGQAGATL VVEELLEGEE
210 220 230 240 250
ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC PCPLISQPAL
260 270 280 290 300
ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE
310 320 330 340 350
TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET
360 370 380 390 400
STKGCLITGL PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL
410 420 430 440 450
KEAAAKATKL AGTITFAGTG AQYRTDIAQK AFKIAIATAP GLSYKDSGVD
460 470 480 490 500
IDAGDALVQR IKPLSRGTQR PGVLGGLGGF GGLFRLKDLS YKEPVIAEAT
510 520 530 540 550
QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA FLDYIACGKL
560 570 580 590 600
HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV
610 620 630 640 650
EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP
660 670 680 690 700
VGGGKHGLSL AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ
710 720 730 740 750
RLLPEGFEVT LDFGAVPVPD VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL
760 770 780 790 800
PQSSLLWRTA LPGAKVLGVL NRQAKASGGA PRVKVRNFVE QLQKLAAPFG
810 820 830 840 850
GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP NHYVDPILIL
860 870 880 890 900
GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG
910 920 930 940 950
KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG
960 970 980 990 1000
IAERSGILPR LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR
1010 1020 1030 1040 1050
APFSEKTLGE ELLVPTKIYV KALSALLSRP NHGIKALAHI TGGGLSENIP
1060 1070 1080 1090 1100
RVLRKELAVR LDANKYPLPP VFAWLAAAGN ISSTELQRTY NCGLGLVLVV
1110 1120 1130 1140 1150
GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF EASLARTQRM
1160 1170 1180 1190 1200
LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL
1210 1220 1230 1240 1250
ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS
1260 1270 1280 1290 1300
VPFVREWRGR LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV
1310 1320 1330 1340 1350
DTGAIIVQAA VPILPGDDEE TLTQRIHYAE HWAFPRALAL LASGALRRVS
1360
EVKKEAPKDI KDSQ
Length:1,364
Mass (Da):145,663
Last modified:June 27, 2006 - v2
Checksum:i4BDF1C7BD435E642
GO
Isoform Short (identifier: P16340-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1364: Missing.

Show »
Length:434
Mass (Da):46,252
Checksum:iB0067502F4A69D52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti327 – 3271L → M in AAX12966. (PubMed:15545653)Curated
Sequence conflicti425 – 4251T → M in AAX12966. (PubMed:15545653)Curated
Sequence conflicti805 – 8051T → S in CAA29611. (PubMed:3123310)Curated
Sequence conflicti956 – 9561G → A in CAA29611. (PubMed:3123310)Curated
Sequence conflicti1145 – 11451A → T in CAA29611. (PubMed:3123310)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei434 – 4341I → M in isoform Short. CuratedVSP_005514
Alternative sequencei435 – 1364930Missing in isoform Short. CuratedVSP_005515Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06285 Genomic DNA. Translation: CAA29611.1.
CH379058 Genomic DNA. Translation: EAL34461.2.
AY754390 Genomic DNA. Translation: AAX12966.1.
PIRiS01204. AJFFPP.
RefSeqiXP_001357392.2. XM_001357356.2. [P16340-1]

Genome annotation databases

EnsemblMetazoaiFBtr0280975; FBpp0279413; FBgn0012717. [P16340-1]
GeneIDi4817813.
KEGGidpo:Dpse_GA25439.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06285 Genomic DNA. Translation: CAA29611.1 .
CH379058 Genomic DNA. Translation: EAL34461.2 .
AY754390 Genomic DNA. Translation: AAX12966.1 .
PIRi S01204. AJFFPP.
RefSeqi XP_001357392.2. XM_001357356.2. [P16340-1 ]

3D structure databases

ProteinModelPortali P16340.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7237.FBpp0279413.

Proteomic databases

PRIDEi P16340.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0280975 ; FBpp0279413 ; FBgn0012717 . [P16340-1 ]
GeneIDi 4817813.
KEGGi dpo:Dpse_GA25439.

Organism-specific databases

FlyBasei FBgn0012717. Dpse\ade3.

Phylogenomic databases

eggNOGi COG0151.
InParanoidi P16340.
KOi K11787.
OrthoDBi EOG7RBZ7H.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00126 .
UPA00074 ; UER00129 .

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPi MF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved arrangement of nested genes at the Drosophila Gart locus."
    Henikoff S., Eghtedarzadeh M.K.
    Genetics 117:711-725(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: EST10.
  2. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.
  3. "Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
    Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
    Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-565.

Entry informationi

Entry nameiPUR2_DROPS
AccessioniPrimary (citable) accession number: P16340
Secondary accession number(s): Q29P31, Q56RW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 27, 2006
Last modified: October 29, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3