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P16340 (PUR2_DROPS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trifunctional purine biosynthetic protein adenosine-3

Including the following 3 domains:

  1. Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name=GARS
    Phosphoribosylglycinamide synthetase
  2. Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
    Alternative name(s):
    AIR synthase
    Short name=AIRS
    Phosphoribosyl-aminoimidazole synthetase
  3. Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name=GART
Gene names
Name:ade3
Synonyms:gart
ORF Names:GA16345
OrganismDrosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]
Taxonomic identifier46245 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1364 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00138

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00138

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the central section; belongs to the AIR synthase family.

In the C-terminal section; belongs to the GART family.

Contains 1 ATP-grasp domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P16340-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P16340-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1364: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13641364Trifunctional purine biosynthetic protein adenosine-3 HAMAP-Rule MF_00138
PRO_0000074935

Regions

Domain114 – 321208ATP-grasp
Nucleotide binding140 – 20263ATP By similarity
Region435 – 1154720AIRS HAMAP-Rule MF_00138
Region1155 – 1364210GART HAMAP-Rule MF_00138
Region1166 – 116835'-phosphoribosylglycinamide binding By similarity
Region1246 – 1249410-formyltetrahydrofolate binding By similarity
Region1297 – 1301510-formyltetrahydrofolate binding By similarity
Region1327 – 133045'-phosphoribosylglycinamide binding By similarity

Sites

Active site12651Proton donor By similarity
Metal binding2911Manganese By similarity
Metal binding2931Manganese By similarity
Binding site1221110-formyltetrahydrofolate By similarity
Binding site1263110-formyltetrahydrofolate By similarity
Site13011Raises pKa of active site His By similarity

Natural variations

Alternative sequence4341I → M in isoform Short.
VSP_005514
Alternative sequence435 – 1364930Missing in isoform Short.
VSP_005515

Experimental info

Sequence conflict3271L → M in AAX12966. Ref.3
Sequence conflict4251T → M in AAX12966. Ref.3
Sequence conflict8051T → S in CAA29611. Ref.1
Sequence conflict9561G → A in CAA29611. Ref.1
Sequence conflict11451A → T in CAA29611. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: 4BDF1C7BD435E642

FASTA1,364145,663
        10         20         30         40         50         60 
MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA KVLDPKDFEA 

        70         80         90        100        110        120 
IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG KQGAQIEADK KWAKDFMLRH 

       130        140        150        160        170        180 
GIPTARYESF TDTNKAKAFI RSAPYQALVV KAAGLAAGKG VVVAANVDEA CQAVDEILGD 

       190        200        210        220        230        240 
LKYGQAGATL VVEELLEGEE ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 

       250        260        270        280        290        300 
PCPLISQPAL ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 

       310        320        330        340        350        360 
TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET STKGCLITGL 

       370        380        390        400        410        420 
PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL KEAAAKATKL AGTITFAGTG 

       430        440        450        460        470        480 
AQYRTDIAQK AFKIAIATAP GLSYKDSGVD IDAGDALVQR IKPLSRGTQR PGVLGGLGGF 

       490        500        510        520        530        540 
GGLFRLKDLS YKEPVIAEAT QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA 

       550        560        570        580        590        600 
FLDYIACGKL HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV 

       610        620        630        640        650        660 
EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP VGGGKHGLSL 

       670        680        690        700        710        720 
AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ RLLPEGFEVT LDFGAVPVPD 

       730        740        750        760        770        780 
VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL PQSSLLWRTA LPGAKVLGVL NRQAKASGGA 

       790        800        810        820        830        840 
PRVKVRNFVE QLQKLAAPFG GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP 

       850        860        870        880        890        900 
NHYVDPILIL GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG 

       910        920        930        940        950        960 
KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG IAERSGILPR 

       970        980        990       1000       1010       1020 
LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR APFSEKTLGE ELLVPTKIYV 

      1030       1040       1050       1060       1070       1080 
KALSALLSRP NHGIKALAHI TGGGLSENIP RVLRKELAVR LDANKYPLPP VFAWLAAAGN 

      1090       1100       1110       1120       1130       1140 
ISSTELQRTY NCGLGLVLVV GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF 

      1150       1160       1170       1180       1190       1200 
EASLARTQRM LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL 

      1210       1220       1230       1240       1250       1260 
ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS VPFVREWRGR 

      1270       1280       1290       1300       1310       1320 
LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV DTGAIIVQAA VPILPGDDEE 

      1330       1340       1350       1360 
TLTQRIHYAE HWAFPRALAL LASGALRRVS EVKKEAPKDI KDSQ 

« Hide

Isoform Short [UniParc].

Checksum: B0067502F4A69D52
Show »

FASTA43446,252

References

« Hide 'large scale' references
[1]"Conserved arrangement of nested genes at the Drosophila Gart locus."
Henikoff S., Eghtedarzadeh M.K.
Genetics 117:711-725(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: EST10.
[2]"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. expand/collapse author list , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MV2-25 / Tucson 14011-0121.94.
[3]"Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-565.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06285 Genomic DNA. Translation: CAA29611.1.
CH379058 Genomic DNA. Translation: EAL34461.2.
AY754390 Genomic DNA. Translation: AAX12966.1.
PIRAJFFPP. S01204.
RefSeqXP_001357392.2. XM_001357356.2.

3D structure databases

ProteinModelPortalP16340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7237.FBpp0279413.

Proteomic databases

PRIDEP16340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0280975; FBpp0279413; FBgn0012717. [P16340-1]
GeneID4817813.
KEGGdpo:Dpse_GA25439.

Organism-specific databases

FlyBaseFBgn0012717. Dpse\ade3.

Phylogenomic databases

eggNOGCOG0151.
InParanoidP16340.
KOK11787.
OrthoDBEOG7RBZ7H.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Family and domain databases

Gene3D3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPMF_00138. GARS.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_DROPS
AccessionPrimary (citable) accession number: P16340
Secondary accession number(s): Q29P31, Q56RW7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 27, 2006
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase