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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

ade3

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (ade3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (Dpse\GA15494), Amidophosphoribosyltransferase (Dpse\GA10055)
  2. Trifunctional purine biosynthetic protein adenosine-3 (ade3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (ade3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291ManganeseBy similarity1
Metal bindingi293ManganeseBy similarity1
Binding sitei122110-formyltetrahydrofolateBy similarity1
Binding sitei126310-formyltetrahydrofolateBy similarity1
Active sitei1265Proton donorBy similarity1
Sitei1301Raises pKa of active site HisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 202ATPBy similarityAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:ade3
Synonyms:gart
ORF Names:GA16345
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001819 Componenti: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0012717. Dpse\ade3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749351 – 1364Trifunctional purine biosynthetic protein adenosine-3Add BLAST1364

Proteomic databases

PRIDEiP16340.

Structurei

3D structure databases

ProteinModelPortaliP16340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 321ATP-graspAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni435 – 1154AIRSAdd BLAST720
Regioni1155 – 1364GARTAdd BLAST210
Regioni1166 – 11685'-phosphoribosylglycinamide bindingBy similarity3
Regioni1246 – 124910-formyltetrahydrofolate bindingBy similarity4
Regioni1297 – 130110-formyltetrahydrofolate bindingBy similarity5
Regioni1327 – 13305'-phosphoribosylglycinamide bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

InParanoidiP16340.
KOiK11787.
OMAiYRTDIAQ.

Family and domain databases

CDDicd08645. FMT_core_GART. 1 hit.
cd02196. PurM. 2 hits.
Gene3Di3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPiMF_00741. AIRS. 1 hit.
MF_00138. GARS. 1 hit.
MF_01930. PurN. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
IPR004607. PurN.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P16340-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA
60 70 80 90 100
KVLDPKDFEA IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG
110 120 130 140 150
KQGAQIEADK KWAKDFMLRH GIPTARYESF TDTNKAKAFI RSAPYQALVV
160 170 180 190 200
KAAGLAAGKG VVVAANVDEA CQAVDEILGD LKYGQAGATL VVEELLEGEE
210 220 230 240 250
ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC PCPLISQPAL
260 270 280 290 300
ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE
310 320 330 340 350
TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET
360 370 380 390 400
STKGCLITGL PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL
410 420 430 440 450
KEAAAKATKL AGTITFAGTG AQYRTDIAQK AFKIAIATAP GLSYKDSGVD
460 470 480 490 500
IDAGDALVQR IKPLSRGTQR PGVLGGLGGF GGLFRLKDLS YKEPVIAEAT
510 520 530 540 550
QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA FLDYIACGKL
560 570 580 590 600
HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV
610 620 630 640 650
EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP
660 670 680 690 700
VGGGKHGLSL AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ
710 720 730 740 750
RLLPEGFEVT LDFGAVPVPD VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL
760 770 780 790 800
PQSSLLWRTA LPGAKVLGVL NRQAKASGGA PRVKVRNFVE QLQKLAAPFG
810 820 830 840 850
GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP NHYVDPILIL
860 870 880 890 900
GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG
910 920 930 940 950
KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG
960 970 980 990 1000
IAERSGILPR LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR
1010 1020 1030 1040 1050
APFSEKTLGE ELLVPTKIYV KALSALLSRP NHGIKALAHI TGGGLSENIP
1060 1070 1080 1090 1100
RVLRKELAVR LDANKYPLPP VFAWLAAAGN ISSTELQRTY NCGLGLVLVV
1110 1120 1130 1140 1150
GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF EASLARTQRM
1160 1170 1180 1190 1200
LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL
1210 1220 1230 1240 1250
ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS
1260 1270 1280 1290 1300
VPFVREWRGR LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV
1310 1320 1330 1340 1350
DTGAIIVQAA VPILPGDDEE TLTQRIHYAE HWAFPRALAL LASGALRRVS
1360
EVKKEAPKDI KDSQ
Length:1,364
Mass (Da):145,663
Last modified:June 27, 2006 - v2
Checksum:i4BDF1C7BD435E642
GO
Isoform Short (identifier: P16340-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1364: Missing.

Show »
Length:434
Mass (Da):46,252
Checksum:iB0067502F4A69D52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti327L → M in AAX12966 (PubMed:15545653).Curated1
Sequence conflicti425T → M in AAX12966 (PubMed:15545653).Curated1
Sequence conflicti805T → S in CAA29611 (PubMed:3123310).Curated1
Sequence conflicti956G → A in CAA29611 (PubMed:3123310).Curated1
Sequence conflicti1145A → T in CAA29611 (PubMed:3123310).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005514434I → M in isoform Short. Curated1
Alternative sequenceiVSP_005515435 – 1364Missing in isoform Short. CuratedAdd BLAST930

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06285 Genomic DNA. Translation: CAA29611.1.
CH379058 Genomic DNA. Translation: EAL34461.2.
AY754390 Genomic DNA. Translation: AAX12966.1.
PIRiS01204. AJFFPP.
RefSeqiXP_001357392.2. XM_001357356.3. [P16340-1]

Genome annotation databases

EnsemblMetazoaiFBtr0280975; FBpp0279413; FBgn0012717. [P16340-1]
GeneIDi4817813.
KEGGidpo:Dpse_GA25439.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06285 Genomic DNA. Translation: CAA29611.1.
CH379058 Genomic DNA. Translation: EAL34461.2.
AY754390 Genomic DNA. Translation: AAX12966.1.
PIRiS01204. AJFFPP.
RefSeqiXP_001357392.2. XM_001357356.3. [P16340-1]

3D structure databases

ProteinModelPortaliP16340.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP16340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0280975; FBpp0279413; FBgn0012717. [P16340-1]
GeneIDi4817813.
KEGGidpo:Dpse_GA25439.

Organism-specific databases

FlyBaseiFBgn0012717. Dpse\ade3.

Phylogenomic databases

InParanoidiP16340.
KOiK11787.
OMAiYRTDIAQ.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Family and domain databases

CDDicd08645. FMT_core_GART. 1 hit.
cd02196. PurM. 2 hits.
Gene3Di3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPiMF_00741. AIRS. 1 hit.
MF_00138. GARS. 1 hit.
MF_01930. PurN. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
IPR004607. PurN.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR2_DROPS
AccessioniPrimary (citable) accession number: P16340
Secondary accession number(s): Q29P31, Q56RW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 27, 2006
Last modified: October 5, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.