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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

ade3

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (ade3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (Dpse\GA15494), Amidophosphoribosyltransferase (Dpse\GA10055)
  2. Trifunctional purine biosynthetic protein adenosine-3 (ade3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (ade3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291ManganeseBy similarity1
Metal bindingi293ManganeseBy similarity1
Binding sitei122110-formyltetrahydrofolateBy similarity1
Binding sitei126310-formyltetrahydrofolateBy similarity1
Active sitei1265Proton donorBy similarity1
Sitei1301Raises pKa of active site HisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 202ATPBy similarityAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125
UPA00074; UER00126
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:ade3
Synonyms:gart
ORF Names:GA16345
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001819 Componenti: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0012717 Dpse\ade3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749351 – 1364Trifunctional purine biosynthetic protein adenosine-3Add BLAST1364

Proteomic databases

PRIDEiP16340

Expressioni

Gene expression databases

BgeeiFBgn0012717

Interactioni

Protein-protein interaction databases

STRINGi7237.FBpp0279413

Structurei

3D structure databases

ProteinModelPortaliP16340
SMRiP16340
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 321ATP-graspAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni435 – 1154AIRSAdd BLAST720
Regioni1155 – 1364GARTAdd BLAST210
Regioni1166 – 11685'-phosphoribosylglycinamide bindingBy similarity3
Regioni1246 – 124910-formyltetrahydrofolate bindingBy similarity4
Regioni1297 – 130110-formyltetrahydrofolate bindingBy similarity5
Regioni1327 – 13305'-phosphoribosylglycinamide bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated

Phylogenomic databases

InParanoidiP16340
KOiK11787
OMAiLLERHNC

Family and domain databases

CDDicd08645 FMT_core_GART, 1 hit
cd02196 PurM, 2 hits
Gene3Di3.30.1330.10, 2 hits
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
MF_01930 PurN, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR002376 Formyl_transf_N
IPR036477 Formyl_transf_N_sf
IPR004607 GART
IPR001555 GART_AS
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PANTHERiPTHR10520 PTHR10520, 2 hits
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PF00551 Formyl_trans_N, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF53328 SSF53328, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 2 hits
TIGR00639 PurN, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit
PS00373 GART, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P16340-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA
60 70 80 90 100
KVLDPKDFEA IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG
110 120 130 140 150
KQGAQIEADK KWAKDFMLRH GIPTARYESF TDTNKAKAFI RSAPYQALVV
160 170 180 190 200
KAAGLAAGKG VVVAANVDEA CQAVDEILGD LKYGQAGATL VVEELLEGEE
210 220 230 240 250
ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC PCPLISQPAL
260 270 280 290 300
ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE
310 320 330 340 350
TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET
360 370 380 390 400
STKGCLITGL PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL
410 420 430 440 450
KEAAAKATKL AGTITFAGTG AQYRTDIAQK AFKIAIATAP GLSYKDSGVD
460 470 480 490 500
IDAGDALVQR IKPLSRGTQR PGVLGGLGGF GGLFRLKDLS YKEPVIAEAT
510 520 530 540 550
QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA FLDYIACGKL
560 570 580 590 600
HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV
610 620 630 640 650
EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP
660 670 680 690 700
VGGGKHGLSL AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ
710 720 730 740 750
RLLPEGFEVT LDFGAVPVPD VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL
760 770 780 790 800
PQSSLLWRTA LPGAKVLGVL NRQAKASGGA PRVKVRNFVE QLQKLAAPFG
810 820 830 840 850
GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP NHYVDPILIL
860 870 880 890 900
GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG
910 920 930 940 950
KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG
960 970 980 990 1000
IAERSGILPR LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR
1010 1020 1030 1040 1050
APFSEKTLGE ELLVPTKIYV KALSALLSRP NHGIKALAHI TGGGLSENIP
1060 1070 1080 1090 1100
RVLRKELAVR LDANKYPLPP VFAWLAAAGN ISSTELQRTY NCGLGLVLVV
1110 1120 1130 1140 1150
GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF EASLARTQRM
1160 1170 1180 1190 1200
LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL
1210 1220 1230 1240 1250
ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS
1260 1270 1280 1290 1300
VPFVREWRGR LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV
1310 1320 1330 1340 1350
DTGAIIVQAA VPILPGDDEE TLTQRIHYAE HWAFPRALAL LASGALRRVS
1360
EVKKEAPKDI KDSQ
Length:1,364
Mass (Da):145,663
Last modified:June 27, 2006 - v2
Checksum:i4BDF1C7BD435E642
GO
Isoform Short (identifier: P16340-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1364: Missing.

Show »
Length:434
Mass (Da):46,252
Checksum:iB0067502F4A69D52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti327L → M in AAX12966 (PubMed:15545653).Curated1
Sequence conflicti425T → M in AAX12966 (PubMed:15545653).Curated1
Sequence conflicti805T → S in CAA29611 (PubMed:3123310).Curated1
Sequence conflicti956G → A in CAA29611 (PubMed:3123310).Curated1
Sequence conflicti1145A → T in CAA29611 (PubMed:3123310).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005514434I → M in isoform Short. Curated1
Alternative sequenceiVSP_005515435 – 1364Missing in isoform Short. CuratedAdd BLAST930

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06285 Genomic DNA Translation: CAA29611.1
CH379058 Genomic DNA Translation: EAL34461.2
AY754390 Genomic DNA Translation: AAX12966.1
PIRiS01204 AJFFPP
RefSeqiXP_001357392.2, XM_001357356.3 [P16340-1]

Genome annotation databases

EnsemblMetazoaiFBtr0280975; FBpp0279413; FBgn0012717 [P16340-1]
GeneIDi4817813
KEGGidpo:Dpse_GA25439

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPUR2_DROPS
AccessioniPrimary (citable) accession number: P16340
Secondary accession number(s): Q29P31, Q56RW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 27, 2006
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health