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P16340

- PUR2_DROPS

UniProt

P16340 - PUR2_DROPS

Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

ade3

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
    10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi291 – 2911ManganeseBy similarity
    Metal bindingi293 – 2931ManganeseBy similarity
    Binding sitei1221 – 1221110-formyltetrahydrofolateBy similarity
    Binding sitei1263 – 1263110-formyltetrahydrofolateBy similarity
    Active sitei1265 – 12651Proton donorBy similarity
    Sitei1301 – 13011Raises pKa of active site HisBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi140 – 20263ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. methyltransferase activity Source: InterPro
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
    5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
    6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00125.
    UPA00074; UER00126.
    UPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trifunctional purine biosynthetic protein adenosine-3
    Including the following 3 domains:
    Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name:
    GART
    Gene namesi
    Name:ade3
    Synonyms:gart
    ORF Names:GA16345
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0012717. Dpse\ade3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13641364Trifunctional purine biosynthetic protein adenosine-3PRO_0000074935Add
    BLAST

    Proteomic databases

    PRIDEiP16340.

    Interactioni

    Protein-protein interaction databases

    STRINGi7237.FBpp0279413.

    Structurei

    3D structure databases

    ProteinModelPortaliP16340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini114 – 321208ATP-graspAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni435 – 1154720AIRSAdd
    BLAST
    Regioni1155 – 1364210GARTAdd
    BLAST
    Regioni1166 – 116835'-phosphoribosylglycinamide bindingBy similarity
    Regioni1246 – 1249410-formyltetrahydrofolate bindingBy similarity
    Regioni1297 – 1301510-formyltetrahydrofolate bindingBy similarity
    Regioni1327 – 133045'-phosphoribosylglycinamide bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the GARS family.Curated
    In the central section; belongs to the AIR synthase family.Curated
    In the C-terminal section; belongs to the GART family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    eggNOGiCOG0151.
    InParanoidiP16340.
    KOiK11787.
    OrthoDBiEOG7RBZ7H.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 2 hits.
    HAMAPiMF_00138. GARS.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    TIGR00878. purM. 2 hits.
    TIGR00639. PurN. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P16340-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA     50
    KVLDPKDFEA IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG 100
    KQGAQIEADK KWAKDFMLRH GIPTARYESF TDTNKAKAFI RSAPYQALVV 150
    KAAGLAAGKG VVVAANVDEA CQAVDEILGD LKYGQAGATL VVEELLEGEE 200
    ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC PCPLISQPAL 250
    ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 300
    TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET 350
    STKGCLITGL PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL 400
    KEAAAKATKL AGTITFAGTG AQYRTDIAQK AFKIAIATAP GLSYKDSGVD 450
    IDAGDALVQR IKPLSRGTQR PGVLGGLGGF GGLFRLKDLS YKEPVIAEAT 500
    QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA FLDYIACGKL 550
    HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV 600
    EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP 650
    VGGGKHGLSL AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ 700
    RLLPEGFEVT LDFGAVPVPD VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL 750
    PQSSLLWRTA LPGAKVLGVL NRQAKASGGA PRVKVRNFVE QLQKLAAPFG 800
    GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP NHYVDPILIL 850
    GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG 900
    KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG 950
    IAERSGILPR LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR 1000
    APFSEKTLGE ELLVPTKIYV KALSALLSRP NHGIKALAHI TGGGLSENIP 1050
    RVLRKELAVR LDANKYPLPP VFAWLAAAGN ISSTELQRTY NCGLGLVLVV 1100
    GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF EASLARTQRM 1150
    LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL 1200
    ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS 1250
    VPFVREWRGR LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV 1300
    DTGAIIVQAA VPILPGDDEE TLTQRIHYAE HWAFPRALAL LASGALRRVS 1350
    EVKKEAPKDI KDSQ 1364
    Length:1,364
    Mass (Da):145,663
    Last modified:June 27, 2006 - v2
    Checksum:i4BDF1C7BD435E642
    GO
    Isoform Short (identifier: P16340-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         434-434: I → M
         435-1364: Missing.

    Show »
    Length:434
    Mass (Da):46,252
    Checksum:iB0067502F4A69D52
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti327 – 3271L → M in AAX12966. (PubMed:15545653)Curated
    Sequence conflicti425 – 4251T → M in AAX12966. (PubMed:15545653)Curated
    Sequence conflicti805 – 8051T → S in CAA29611. (PubMed:3123310)Curated
    Sequence conflicti956 – 9561G → A in CAA29611. (PubMed:3123310)Curated
    Sequence conflicti1145 – 11451A → T in CAA29611. (PubMed:3123310)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei434 – 4341I → M in isoform Short. CuratedVSP_005514
    Alternative sequencei435 – 1364930Missing in isoform Short. CuratedVSP_005515Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06285 Genomic DNA. Translation: CAA29611.1.
    CH379058 Genomic DNA. Translation: EAL34461.2.
    AY754390 Genomic DNA. Translation: AAX12966.1.
    PIRiS01204. AJFFPP.
    RefSeqiXP_001357392.2. XM_001357356.2. [P16340-1]

    Genome annotation databases

    EnsemblMetazoaiFBtr0280975; FBpp0279413; FBgn0012717. [P16340-1]
    GeneIDi4817813.
    KEGGidpo:Dpse_GA25439.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06285 Genomic DNA. Translation: CAA29611.1 .
    CH379058 Genomic DNA. Translation: EAL34461.2 .
    AY754390 Genomic DNA. Translation: AAX12966.1 .
    PIRi S01204. AJFFPP.
    RefSeqi XP_001357392.2. XM_001357356.2. [P16340-1 ]

    3D structure databases

    ProteinModelPortali P16340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7237.FBpp0279413.

    Proteomic databases

    PRIDEi P16340.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0280975 ; FBpp0279413 ; FBgn0012717 . [P16340-1 ]
    GeneIDi 4817813.
    KEGGi dpo:Dpse_GA25439.

    Organism-specific databases

    FlyBasei FBgn0012717. Dpse\ade3.

    Phylogenomic databases

    eggNOGi COG0151.
    InParanoidi P16340.
    KOi K11787.
    OrthoDBi EOG7RBZ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    UPA00074 ; UER00126 .
    UPA00074 ; UER00129 .

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 2 hits.
    HAMAPi MF_00138. GARS.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    TIGR00878. purM. 2 hits.
    TIGR00639. PurN. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conserved arrangement of nested genes at the Drosophila Gart locus."
      Henikoff S., Eghtedarzadeh M.K.
      Genetics 117:711-725(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: EST10.
    2. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.
    3. "Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
      Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
      Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-565.

    Entry informationi

    Entry nameiPUR2_DROPS
    AccessioniPrimary (citable) accession number: P16340
    Secondary accession number(s): Q29P31, Q56RW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3