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Protein

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Gene

Cnp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.1 Publication

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei250Proton acceptor1 Publication1
Binding sitei252Substrate1
Active sitei329Proton donor1 Publication1
Binding sitei331Substrate1

GO - Molecular functioni

GO - Biological processi

  • adult locomotory behavior Source: MGI
  • aging Source: Ensembl
  • axonogenesis Source: MGI
  • cyclic nucleotide catabolic process Source: InterPro
  • forebrain development Source: Ensembl
  • microtubule cytoskeleton organization Source: Ensembl
  • oligodendrocyte differentiation Source: MGI
  • regulation of mitochondrial membrane permeability Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to toxic substance Source: MGI
  • substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 3'-phosphodiesterase (EC:3.1.4.37)
Short name:
CNP
Short name:
CNPase
Gene namesi
Name:Cnp
Synonyms:Cnp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88437. Cnp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899621 – 4172',3'-cyclic-nucleotide 3'-phosphodiesteraseAdd BLAST417
PropeptideiPRO_0000422297418 – 420Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei110PhosphotyrosineCombined sources1
Modified residuei169PhosphoserineCombined sources1
Modified residuei227PhosphoserineBy similarity1
Modified residuei239PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1
Modified residuei417Cysteine methyl esterCurated1
Lipidationi417S-farnesyl cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP16330.
PeptideAtlasiP16330.
PRIDEiP16330.

PTM databases

iPTMnetiP16330.
PhosphoSitePlusiP16330.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006782.
CleanExiMM_CNP.
ExpressionAtlasiP16330. baseline and differential.
GenevisibleiP16330. MM.

Interactioni

Subunit structurei

Exists as monomers and homodimers.1 Publication

Protein-protein interaction databases

BioGridi198792. 4 interactors.
IntActiP16330. 12 interactors.
MINTiMINT-1955077.
STRINGi10090.ENSMUSP00000099409.

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi187 – 193Combined sources7
Helixi195 – 214Combined sources20
Helixi216 – 220Combined sources5
Helixi221 – 224Combined sources4
Helixi237 – 240Combined sources4
Beta strandi246 – 248Combined sources3
Beta strandi250 – 255Combined sources6
Helixi257 – 259Combined sources3
Helixi264 – 269Combined sources6
Helixi271 – 276Combined sources6
Beta strandi280 – 290Combined sources11
Beta strandi292 – 300Combined sources9
Helixi303 – 306Combined sources4
Helixi317 – 319Combined sources3
Beta strandi320 – 322Combined sources3
Turni324 – 327Combined sources4
Beta strandi329 – 334Combined sources6
Helixi342 – 354Combined sources13
Turni356 – 359Combined sources4
Beta strandi364 – 366Combined sources3
Beta strandi369 – 375Combined sources7
Beta strandi378 – 396Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XMIX-ray1.74A179-398[»]
2Y1PX-ray1.82A179-398[»]
2Y3XX-ray2.10A/B/E179-398[»]
2YDBX-ray2.15A179-398[»]
2YDCX-ray2.05A179-398[»]
2YDDX-ray2.40A179-398[»]
2YOZX-ray2.10A179-398[»]
2YP0X-ray2.30A179-398[»]
2YPCX-ray1.89A179-398[»]
2YPEX-ray1.90A179-398[»]
2YPHX-ray2.10A179-398[»]
2YQ9X-ray1.90A179-398[»]
3ZBRX-ray2.30A/B179-398[»]
3ZBSX-ray2.45A179-398[»]
3ZBZX-ray2.10A179-398[»]
4WBIX-ray2.00A179-398[»]
4WBLX-ray2.50A179-398[»]
4WC9X-ray2.00A179-398[»]
4WCAX-ray1.85A179-398[»]
4WCBX-ray1.57A179-398[»]
4WCCX-ray2.70A179-398[»]
4WDAX-ray1.85A179-398[»]
4WDBX-ray1.60A179-398[»]
4WDDX-ray2.10A179-398[»]
4WDEX-ray2.40A/B179-398[»]
4WDFX-ray2.00A179-398[»]
4WDGX-ray2.05A179-398[»]
4WDHX-ray1.90A179-398[»]
4WEXX-ray2.10A179-398[»]
4WFRX-ray2.00A179-398[»]
5AE0X-ray1.04A179-398[»]
ProteinModelPortaliP16330.
SMRiP16330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IT06. Eukaryota.
ENOG4111F3B. LUCA.
GeneTreeiENSGT00510000048410.
HOGENOMiHOG000111838.
HOVERGENiHBG001451.
InParanoidiP16330.
KOiK01121.
OMAiLWPNDVD.
OrthoDBiEOG091G0I8Z.
PhylomeDBiP16330.
TreeFamiTF332157.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008431. CNPase.
IPR027417. P-loop_NTPase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PANTHERiPTHR10156. PTHR10156. 1 hit.
PfamiPF05881. CNPase. 1 hit.
[Graphical view]
PIRSFiPIRSF000970. CNPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55144. SSF55144. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CNPII (identifier: P16330-1) [UniParc]FASTAAdd to basket
Also known as: DNAII

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTSFTRKSH TFLPKLFFRK MSSSGAKEKP ELQFPFLQDE DTVATLHECK
60 70 80 90 100
TLFILRGLPG SGKSTLARLI LEKYHDGTKM VSADAYKIIP GSRADFSEAY
110 120 130 140 150
KRLDEDLAGY CRRDIRVLVL DDTNHERERL DQLFEMADQY QYQVVLVEPK
160 170 180 190 200
TAWRLDCAQL KEKNQWQLSA DDLKKLKPGL EKDFLPLYFG WFLTKKSSET
210 220 230 240 250
LRKAGQVFLE ELGNHKAFKK ELRHFISGDE PKEKLELVSY FGKRPPGVLH
260 270 280 290 300
CTTKFCDYGK AAGAEEYAQQ EVVKRSYGKA FKLSISALFV TPKTAGAQVV
310 320 330 340 350
LTDQELQLWP SDLDKPSASE GLPPGSRAHV TLGCAADVQP VQTGLDLLDI
360 370 380 390 400
LQQVKGGSQG EAVGELPRGK LYSLGKGRWM LSLTKKMEVK AIFTGYYGKG
410 420
KPVPIHGSRK GGAMQICTII
Length:420
Mass (Da):47,123
Last modified:August 16, 2004 - v3
Checksum:i2FAFFDE5F0E99EAB
GO
Isoform CNPI (identifier: P16330-2) [UniParc]FASTAAdd to basket
Also known as: DNAI

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:400
Mass (Da):44,654
Checksum:iB974404499DE29BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115I → M in BAA07621 (PubMed:2558653).Curated1
Sequence conflicti115I → M in BAA07622 (PubMed:2558653).Curated1
Sequence conflicti136M → L in BAA07621 (PubMed:2558653).Curated1
Sequence conflicti136M → L in BAA07622 (PubMed:2558653).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0041721 – 20Missing in isoform CNPI. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38642 Genomic DNA. Translation: BAA07621.1.
D38642 Genomic DNA. Translation: BAA07622.1.
M31810 mRNA. Translation: AAA37429.1.
M58045 mRNA. Translation: AAA37430.1.
M58047, M58046 Genomic DNA. Translation: AAA37431.1.
AF332055 mRNA. Translation: AAK56084.1.
AF332056 mRNA. Translation: AAK56085.1.
BC005544 mRNA. Translation: AAH05544.1.
BC021904 mRNA. Translation: AAH21904.1.
AK050628 mRNA. Translation: BAC34351.1.
CCDSiCCDS25427.1. [P16330-1]
PIRiA35708. ESMS32.
RefSeqiNP_001139790.1. NM_001146318.1. [P16330-2]
NP_034053.2. NM_009923.2. [P16330-1]
XP_017169734.1. XM_017314245.1. [P16330-2]
UniGeneiMm.15711.

Genome annotation databases

EnsembliENSMUST00000103120; ENSMUSP00000099409; ENSMUSG00000006782. [P16330-1]
GeneIDi12799.
KEGGimmu:12799.
UCSCiuc007llo.2. mouse. [P16330-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38642 Genomic DNA. Translation: BAA07621.1.
D38642 Genomic DNA. Translation: BAA07622.1.
M31810 mRNA. Translation: AAA37429.1.
M58045 mRNA. Translation: AAA37430.1.
M58047, M58046 Genomic DNA. Translation: AAA37431.1.
AF332055 mRNA. Translation: AAK56084.1.
AF332056 mRNA. Translation: AAK56085.1.
BC005544 mRNA. Translation: AAH05544.1.
BC021904 mRNA. Translation: AAH21904.1.
AK050628 mRNA. Translation: BAC34351.1.
CCDSiCCDS25427.1. [P16330-1]
PIRiA35708. ESMS32.
RefSeqiNP_001139790.1. NM_001146318.1. [P16330-2]
NP_034053.2. NM_009923.2. [P16330-1]
XP_017169734.1. XM_017314245.1. [P16330-2]
UniGeneiMm.15711.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XMIX-ray1.74A179-398[»]
2Y1PX-ray1.82A179-398[»]
2Y3XX-ray2.10A/B/E179-398[»]
2YDBX-ray2.15A179-398[»]
2YDCX-ray2.05A179-398[»]
2YDDX-ray2.40A179-398[»]
2YOZX-ray2.10A179-398[»]
2YP0X-ray2.30A179-398[»]
2YPCX-ray1.89A179-398[»]
2YPEX-ray1.90A179-398[»]
2YPHX-ray2.10A179-398[»]
2YQ9X-ray1.90A179-398[»]
3ZBRX-ray2.30A/B179-398[»]
3ZBSX-ray2.45A179-398[»]
3ZBZX-ray2.10A179-398[»]
4WBIX-ray2.00A179-398[»]
4WBLX-ray2.50A179-398[»]
4WC9X-ray2.00A179-398[»]
4WCAX-ray1.85A179-398[»]
4WCBX-ray1.57A179-398[»]
4WCCX-ray2.70A179-398[»]
4WDAX-ray1.85A179-398[»]
4WDBX-ray1.60A179-398[»]
4WDDX-ray2.10A179-398[»]
4WDEX-ray2.40A/B179-398[»]
4WDFX-ray2.00A179-398[»]
4WDGX-ray2.05A179-398[»]
4WDHX-ray1.90A179-398[»]
4WEXX-ray2.10A179-398[»]
4WFRX-ray2.00A179-398[»]
5AE0X-ray1.04A179-398[»]
ProteinModelPortaliP16330.
SMRiP16330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198792. 4 interactors.
IntActiP16330. 12 interactors.
MINTiMINT-1955077.
STRINGi10090.ENSMUSP00000099409.

PTM databases

iPTMnetiP16330.
PhosphoSitePlusiP16330.

Proteomic databases

PaxDbiP16330.
PeptideAtlasiP16330.
PRIDEiP16330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103120; ENSMUSP00000099409; ENSMUSG00000006782. [P16330-1]
GeneIDi12799.
KEGGimmu:12799.
UCSCiuc007llo.2. mouse. [P16330-1]

Organism-specific databases

CTDi1267.
MGIiMGI:88437. Cnp.

Phylogenomic databases

eggNOGiENOG410IT06. Eukaryota.
ENOG4111F3B. LUCA.
GeneTreeiENSGT00510000048410.
HOGENOMiHOG000111838.
HOVERGENiHBG001451.
InParanoidiP16330.
KOiK01121.
OMAiLWPNDVD.
OrthoDBiEOG091G0I8Z.
PhylomeDBiP16330.
TreeFamiTF332157.

Miscellaneous databases

ChiTaRSiCnp. mouse.
PROiP16330.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006782.
CleanExiMM_CNP.
ExpressionAtlasiP16330. baseline and differential.
GenevisibleiP16330. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008431. CNPase.
IPR027417. P-loop_NTPase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PANTHERiPTHR10156. PTHR10156. 1 hit.
PfamiPF05881. CNPase. 1 hit.
[Graphical view]
PIRSFiPIRSF000970. CNPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55144. SSF55144. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCN37_MOUSE
AccessioniPrimary (citable) accession number: P16330
Secondary accession number(s): Q61424
, Q8C7C9, Q91V42, Q923F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.