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P16283

- B3A3_MOUSE

UniProt

P16283 - B3A3_MOUSE

Protein

Anion exchange protein 3

Gene

Slc4a3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Plasma membrane anion exchange protein.

    GO - Molecular functioni

    1. anion:anion antiporter activity Source: MGI
    2. inorganic anion exchanger activity Source: InterPro

    GO - Biological processi

    1. regulation of intracellular pH Source: MGI

    Keywords - Biological processi

    Anion exchange, Antiport, Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anion exchange protein 3
    Short name:
    AE 3
    Short name:
    Anion exchanger 3
    Alternative name(s):
    Neuronal band 3-like protein
    Solute carrier family 4 member 3
    Gene namesi
    Name:Slc4a3
    Synonyms:Ae3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:109350. Slc4a3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12271227Anion exchange protein 3PRO_0000079220Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi868 – 8681N-linked (GlcNAc...)Sequence Analysis
    Lipidationi1160 – 11601S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP16283.
    PRIDEiP16283.

    PTM databases

    PhosphoSiteiP16283.

    Expressioni

    Tissue specificityi

    Neuronal.

    Gene expression databases

    ArrayExpressiP16283.
    BgeeiP16283.
    GenevestigatoriP16283.

    Interactioni

    Protein-protein interaction databases

    IntActiP16283. 1 interaction.
    MINTiMINT-4996490.

    Structurei

    3D structure databases

    ProteinModelPortaliP16283.
    SMRiP16283. Positions 319-637, 691-732.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 707707CytoplasmicAdd
    BLAST
    Topological domaini906 – 92015CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei708 – 73023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei736 – 77338HelicalSequence AnalysisAdd
    BLAST
    Transmembranei793 – 81523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei825 – 84622HelicalSequence AnalysisAdd
    BLAST
    Transmembranei888 – 90518HelicalSequence AnalysisAdd
    BLAST
    Transmembranei921 – 94121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei975 – 99723HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1023 – 104422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1078 – 112346HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1150 – 118637HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni708 – 1227520Membrane (anion exchange)Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi135 – 14511Poly-GluAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268067.
    GeneTreeiENSGT00560000076933.
    HOGENOMiHOG000280683.
    HOVERGENiHBG004326.
    KOiK13856.
    OMAiGDSHRGK.
    OrthoDBiEOG7TMZR0.
    PhylomeDBiP16283.
    TreeFamiTF313630.

    Family and domain databases

    Gene3Di3.40.1100.10. 1 hit.
    InterProiIPR001717. Anion_exchange.
    IPR002979. Anion_exchange_3.
    IPR018241. Anion_exchange_CS.
    IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view]
    PANTHERiPTHR11453. PTHR11453. 1 hit.
    PTHR11453:SF15. PTHR11453:SF15. 1 hit.
    PfamiPF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 2 hits.
    [Graphical view]
    PRINTSiPR00165. ANIONEXCHNGR.
    PR01189. ANIONEXHNGR3.
    PR01231. HCO3TRNSPORT.
    SUPFAMiSSF55804. SSF55804. 2 hits.
    TIGRFAMsiTIGR00834. ae. 1 hit.
    PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
    PS00220. ANION_EXCHANGER_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform FL-AE3 (identifier: P16283-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MANGVIPPPG GASPLPQVRV PLEEPPLGPD VEEEDDDLGK TLAVSRFGDL     50
    ISKTPAWDPE KPSRSYSERD FEFHRHTSHH THHPLSARLP PPHKLRRPPP 100
    TSARHTRRKR KKEKTSAPPS EGTPPIQEEG GAGAEEEEEE EEEEEGESEA 150
    EPVEPLPPGP PQKAKFSIGS DEDDSPGLPV KAPCAKALPS VGLQSDQSPQ 200
    RSGSSPSPRA RASRISTEKS RPWSPSASYD LRERLCPGSA LGNPGPEQRV 250
    PTDEAEAQML GSADLDDMKS HRLEDNPGVR RHLVKKPSRI QGGRGSPSGL 300
    APILRRKKKK KKLDRRPHEV FVELNELMLD RSQEPHWRET ARWIKFEEDV 350
    EEETERWGKP HVASLSFRSL LELRRTIAQG AALLDLEQTT LPGIAHLVVE 400
    TMIVSDQIRP EDRASVLRTL LLKHSHPNDD KDSGFFPRNP SSSSVNSVLG 450
    NHHPTPSHGP DGAVPTMADD QGEPAPLWPH DPDAKEKPLH MPGGDGHRGK 500
    SLKLLEKIPE DAEATVVLVG CVPFLEQPAA AFVRLSEAVL LESVLEVPVP 550
    VRFLFVMLGP SHTSTDYHEL GRSIATLMSD KLFHEAAYQA DDRQDLLGAI 600
    SEFLDGSIVI PPSEVEGRDL LRSVAAFQRE LLRKRREREQ TKVEMTTRGG 650
    YAAPGKELSL EMGGSEATSE DDPLQRTGSV FGGLVRDVKR RYPHYPSDLR 700
    DALHSQCVAA VLFIYFAALS PAITFGGLLG EKTEGLMGVS ELIVSTAVLG 750
    VLFSLLGAQP LLVVGFSGPL LVFEEAFFKF CRAQDLEYLT GRVWVGLWLV 800
    VFVLALVAAE GSFLVRYISP FTQEIFAFLI SLIFIYETFH KLYKVFTEHP 850
    LLPFYPPDEA LETGLELNSS ALPPTEGPPG PRNQPNTALL SLILMLGTFL 900
    IAFFLRKFRN SRFLGGKARR IIGDFGIPIS ILVMVLVDYS ITDTYTQKLT 950
    VPTGLSVTSP HKRTWFIPPL GSARPFPPWM MVAAAVPALL VLILIFMETQ 1000
    ITALIVSQKA RRLLKGSGFH LDLLLIGSLG GLCGLFGLPW LTAATVRSVT 1050
    HVNALTVMRT AIAPGDKPQI QEVREQRVTG VLIASLVGLS IVMGAVLRRI 1100
    PLAVLFGIFL YMGVTSLSGI QLSQRLLLIF MPAKHHPEQP YVTKVKTWRM 1150
    HLFTCIQLGC IALLWVVKST AASLAFPFLL LLTVPLRRCL LPRLFQDREL 1200
    QALDSEDAEP NFDEDGQDEY NELHMPV 1227
    Length:1,227
    Mass (Da):135,373
    Last modified:July 27, 2011 - v2
    Checksum:i68BC64B72E5FDC55
    GO
    Isoform 311-AE3 (identifier: P16283-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         381-391: AALLDLEQTTL → RAFWAGNESLL
         392-1227: Missing.

    Show »
    Length:391
    Mass (Da):43,470
    Checksum:i3489399BE07290D5
    GO
    Isoform 14-AE3 (identifier: P16283-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         487-503: KPLHMPGGDGHRGKSLK → FCVLRSPSPCLGETVTEGKA
         504-1227: Missing.

    Show »
    Length:506
    Mass (Da):55,695
    Checksum:iB9749CF90C91B695
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti530 – 5301A → G in AAA37184. (PubMed:2686841)Curated
    Sequence conflicti812 – 8121S → T in AAA37184. (PubMed:2686841)Curated
    Sequence conflicti1150 – 11512MH → ID in AAA37184. (PubMed:2686841)Curated
    Sequence conflicti1187 – 11882RR → SG in AAA37184. (PubMed:2686841)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei381 – 39111AALLDLEQTTL → RAFWAGNESLL in isoform 311-AE3. CuratedVSP_000464Add
    BLAST
    Alternative sequencei392 – 1227836Missing in isoform 311-AE3. CuratedVSP_000465Add
    BLAST
    Alternative sequencei487 – 50317KPLHM…GKSLK → FCVLRSPSPCLGETVTEGKA in isoform 14-AE3. CuratedVSP_000466Add
    BLAST
    Alternative sequencei504 – 1227724Missing in isoform 14-AE3. CuratedVSP_000467Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28383 mRNA. Translation: AAA37184.1.
    S69314 mRNA. Translation: AAB30140.1.
    AF294651 Genomic DNA. Translation: AAG25582.1.
    CH466548 Genomic DNA. Translation: EDL00426.1.
    CCDSiCCDS15080.1. [P16283-1]
    PIRiA33638.
    RefSeqiNP_033234.2. NM_009208.3. [P16283-1]
    XP_006496512.1. XM_006496449.1. [P16283-1]
    XP_006496513.1. XM_006496450.1. [P16283-1]
    XP_006496514.1. XM_006496451.1. [P16283-1]
    XP_006496515.1. XM_006496452.1. [P16283-1]
    XP_006496516.1. XM_006496453.1. [P16283-1]
    UniGeneiMm.5053.

    Genome annotation databases

    EnsembliENSMUST00000124341; ENSMUSP00000116747; ENSMUSG00000006576. [P16283-1]
    GeneIDi20536.
    KEGGimmu:20536.
    UCSCiuc007bpv.2. mouse. [P16283-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28383 mRNA. Translation: AAA37184.1 .
    S69314 mRNA. Translation: AAB30140.1 .
    AF294651 Genomic DNA. Translation: AAG25582.1 .
    CH466548 Genomic DNA. Translation: EDL00426.1 .
    CCDSi CCDS15080.1. [P16283-1 ]
    PIRi A33638.
    RefSeqi NP_033234.2. NM_009208.3. [P16283-1 ]
    XP_006496512.1. XM_006496449.1. [P16283-1 ]
    XP_006496513.1. XM_006496450.1. [P16283-1 ]
    XP_006496514.1. XM_006496451.1. [P16283-1 ]
    XP_006496515.1. XM_006496452.1. [P16283-1 ]
    XP_006496516.1. XM_006496453.1. [P16283-1 ]
    UniGenei Mm.5053.

    3D structure databases

    ProteinModelPortali P16283.
    SMRi P16283. Positions 319-637, 691-732.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P16283. 1 interaction.
    MINTi MINT-4996490.

    PTM databases

    PhosphoSitei P16283.

    Proteomic databases

    PaxDbi P16283.
    PRIDEi P16283.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000124341 ; ENSMUSP00000116747 ; ENSMUSG00000006576 . [P16283-1 ]
    GeneIDi 20536.
    KEGGi mmu:20536.
    UCSCi uc007bpv.2. mouse. [P16283-1 ]

    Organism-specific databases

    CTDi 6508.
    MGIi MGI:109350. Slc4a3.

    Phylogenomic databases

    eggNOGi NOG268067.
    GeneTreei ENSGT00560000076933.
    HOGENOMi HOG000280683.
    HOVERGENi HBG004326.
    KOi K13856.
    OMAi GDSHRGK.
    OrthoDBi EOG7TMZR0.
    PhylomeDBi P16283.
    TreeFami TF313630.

    Miscellaneous databases

    NextBioi 298807.
    PROi P16283.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P16283.
    Bgeei P16283.
    Genevestigatori P16283.

    Family and domain databases

    Gene3Di 3.40.1100.10. 1 hit.
    InterProi IPR001717. Anion_exchange.
    IPR002979. Anion_exchange_3.
    IPR018241. Anion_exchange_CS.
    IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view ]
    PANTHERi PTHR11453. PTHR11453. 1 hit.
    PTHR11453:SF15. PTHR11453:SF15. 1 hit.
    Pfami PF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 2 hits.
    [Graphical view ]
    PRINTSi PR00165. ANIONEXCHNGR.
    PR01189. ANIONEXHNGR3.
    PR01231. HCO3TRNSPORT.
    SUPFAMi SSF55804. SSF55804. 2 hits.
    TIGRFAMsi TIGR00834. ae. 1 hit.
    PROSITEi PS00219. ANION_EXCHANGER_1. 1 hit.
    PS00220. ANION_EXCHANGER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger."
      Kopito R.R., Lee B.S., Simmons D.M., Lindsey A.E., Morgans C.W., Schneider K.
      Cell 59:927-937(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Generation of truncated brain AE3 isoforms by alternate mRNA processing."
      Morgans C.W., Kopito R.R.
      J. Cell Sci. 106:1275-1282(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    3. "Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene."
      Iwaasa M., Tatewaki H., Ohno T., Okubo K., Hamasaki N., Kang D.
      DNA Seq. 13:251-255(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiB3A3_MOUSE
    AccessioniPrimary (citable) accession number: P16283
    Secondary accession number(s): Q9ERP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3