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P16283 (B3A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anion exchange protein 3
Short name=AE 3
Short name=Anion exchanger 3
Alternative name(s):
Neuronal band 3-like protein
Solute carrier family 4 member 3
Gene names
Name:Slc4a3
Synonyms:Ae3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1227 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plasma membrane anion exchange protein.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Neuronal.

Sequence similarities

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform FL-AE3 (identifier: P16283-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 311-AE3 (identifier: P16283-2)

The sequence of this isoform differs from the canonical sequence as follows:
     381-391: AALLDLEQTTL → RAFWAGNESLL
     392-1227: Missing.
Isoform 14-AE3 (identifier: P16283-3)

The sequence of this isoform differs from the canonical sequence as follows:
     487-503: KPLHMPGGDGHRGKSLK → FCVLRSPSPCLGETVTEGKA
     504-1227: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12271227Anion exchange protein 3
PRO_0000079220

Regions

Topological domain1 – 707707Cytoplasmic
Transmembrane708 – 73023Helical; Potential
Transmembrane736 – 77338Helical; Potential
Transmembrane793 – 81523Helical; Potential
Transmembrane825 – 84622Helical; Potential
Transmembrane888 – 90518Helical; Potential
Topological domain906 – 92015Cytoplasmic Potential
Transmembrane921 – 94121Helical; Potential
Transmembrane975 – 99723Helical; Potential
Transmembrane1023 – 104422Helical; Potential
Transmembrane1078 – 112346Helical; Potential
Transmembrane1150 – 118637Helical; Potential
Region708 – 1227520Membrane (anion exchange)
Compositional bias135 – 14511Poly-Glu

Amino acid modifications

Modified residue1701Phosphoserine Ref.5
Modified residue1981Phosphoserine Ref.5
Modified residue2961Phosphoserine Ref.5
Lipidation11601S-palmitoyl cysteine By similarity
Glycosylation8681N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence381 – 39111AALLDLEQTTL → RAFWAGNESLL in isoform 311-AE3.
VSP_000464
Alternative sequence392 – 1227836Missing in isoform 311-AE3.
VSP_000465
Alternative sequence487 – 50317KPLHM…GKSLK → FCVLRSPSPCLGETVTEGKA in isoform 14-AE3.
VSP_000466
Alternative sequence504 – 1227724Missing in isoform 14-AE3.
VSP_000467

Experimental info

Sequence conflict5301A → G in AAA37184. Ref.1
Sequence conflict8121S → T in AAA37184. Ref.1
Sequence conflict1150 – 11512MH → ID in AAA37184. Ref.1
Sequence conflict1187 – 11882RR → SG in AAA37184. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform FL-AE3 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 68BC64B72E5FDC55

FASTA1,227135,373
        10         20         30         40         50         60 
MANGVIPPPG GASPLPQVRV PLEEPPLGPD VEEEDDDLGK TLAVSRFGDL ISKTPAWDPE 

        70         80         90        100        110        120 
KPSRSYSERD FEFHRHTSHH THHPLSARLP PPHKLRRPPP TSARHTRRKR KKEKTSAPPS 

       130        140        150        160        170        180 
EGTPPIQEEG GAGAEEEEEE EEEEEGESEA EPVEPLPPGP PQKAKFSIGS DEDDSPGLPV 

       190        200        210        220        230        240 
KAPCAKALPS VGLQSDQSPQ RSGSSPSPRA RASRISTEKS RPWSPSASYD LRERLCPGSA 

       250        260        270        280        290        300 
LGNPGPEQRV PTDEAEAQML GSADLDDMKS HRLEDNPGVR RHLVKKPSRI QGGRGSPSGL 

       310        320        330        340        350        360 
APILRRKKKK KKLDRRPHEV FVELNELMLD RSQEPHWRET ARWIKFEEDV EEETERWGKP 

       370        380        390        400        410        420 
HVASLSFRSL LELRRTIAQG AALLDLEQTT LPGIAHLVVE TMIVSDQIRP EDRASVLRTL 

       430        440        450        460        470        480 
LLKHSHPNDD KDSGFFPRNP SSSSVNSVLG NHHPTPSHGP DGAVPTMADD QGEPAPLWPH 

       490        500        510        520        530        540 
DPDAKEKPLH MPGGDGHRGK SLKLLEKIPE DAEATVVLVG CVPFLEQPAA AFVRLSEAVL 

       550        560        570        580        590        600 
LESVLEVPVP VRFLFVMLGP SHTSTDYHEL GRSIATLMSD KLFHEAAYQA DDRQDLLGAI 

       610        620        630        640        650        660 
SEFLDGSIVI PPSEVEGRDL LRSVAAFQRE LLRKRREREQ TKVEMTTRGG YAAPGKELSL 

       670        680        690        700        710        720 
EMGGSEATSE DDPLQRTGSV FGGLVRDVKR RYPHYPSDLR DALHSQCVAA VLFIYFAALS 

       730        740        750        760        770        780 
PAITFGGLLG EKTEGLMGVS ELIVSTAVLG VLFSLLGAQP LLVVGFSGPL LVFEEAFFKF 

       790        800        810        820        830        840 
CRAQDLEYLT GRVWVGLWLV VFVLALVAAE GSFLVRYISP FTQEIFAFLI SLIFIYETFH 

       850        860        870        880        890        900 
KLYKVFTEHP LLPFYPPDEA LETGLELNSS ALPPTEGPPG PRNQPNTALL SLILMLGTFL 

       910        920        930        940        950        960 
IAFFLRKFRN SRFLGGKARR IIGDFGIPIS ILVMVLVDYS ITDTYTQKLT VPTGLSVTSP 

       970        980        990       1000       1010       1020 
HKRTWFIPPL GSARPFPPWM MVAAAVPALL VLILIFMETQ ITALIVSQKA RRLLKGSGFH 

      1030       1040       1050       1060       1070       1080 
LDLLLIGSLG GLCGLFGLPW LTAATVRSVT HVNALTVMRT AIAPGDKPQI QEVREQRVTG 

      1090       1100       1110       1120       1130       1140 
VLIASLVGLS IVMGAVLRRI PLAVLFGIFL YMGVTSLSGI QLSQRLLLIF MPAKHHPEQP 

      1150       1160       1170       1180       1190       1200 
YVTKVKTWRM HLFTCIQLGC IALLWVVKST AASLAFPFLL LLTVPLRRCL LPRLFQDREL 

      1210       1220 
QALDSEDAEP NFDEDGQDEY NELHMPV

« Hide

Isoform 311-AE3 [UniParc].

Checksum: 3489399BE07290D5
Show »

FASTA39143,470
Isoform 14-AE3 [UniParc].

Checksum: B9749CF90C91B695
Show »

FASTA50655,695

References

« Hide 'large scale' references
[1]"Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger."
Kopito R.R., Lee B.S., Simmons D.M., Lindsey A.E., Morgans C.W., Schneider K.
Cell 59:927-937(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Generation of truncated brain AE3 isoforms by alternate mRNA processing."
Morgans C.W., Kopito R.R.
J. Cell Sci. 106:1275-1282(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[3]"Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene."
Iwaasa M., Tatewaki H., Ohno T., Okubo K., Hamasaki N., Kang D.
DNA Seq. 13:251-255(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170; SER-198 AND SER-296, MASS SPECTROMETRY.
Tissue: Brain cortex.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M28383 mRNA. Translation: AAA37184.1.
S69314 mRNA. Translation: AAB30140.1.
AF294651 Genomic DNA. Translation: AAG25582.1.
CH466548 Genomic DNA. Translation: EDL00426.1.
IPIIPI00875624.
IPI00919051.
IPI01023114.
PIRA33638.
RefSeqNP_033234.2. NM_009208.3.
UniGeneMm.5053.

3D structure databases

HSSPHSSP built from PDB template 1BTQ based on UniProtKB P02730.
ProteinModelPortalP16283.
SMRP16283. Positions 691-732.
ModBaseSearch...

Protein-protein interaction databases

IntActP16283. 1 interaction.

PTM databases

PhosphoSiteP16283.

Proteomic databases

PaxDbP16283.
PRIDEP16283.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000124341; ENSMUSP00000116747; ENSMUSG00000006576.
GeneID20536.
KEGGmmu:20536.

Organism-specific databases

CTD6508.
MGIMGI:109350. Slc4a3.

Phylogenomic databases

eggNOGNOG268067.
GeneTreeENSGT00560000076933.
HOGENOMHOG000280683.
HOVERGENHBG004326.
KOK13856.
OMAGRAAFTK.

Gene expression databases

ArrayExpressP16283.
BgeeP16283.
GenevestigatorP16283.
GermOnlineENSMUSG00000006576. Mus musculus.

Family and domain databases

Gene3D3.40.1100.10. 1 hit.
InterProIPR001717. Anion_exchange.
IPR002979. Anion_exchange_3.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERPTHR11453. PTHR11453. 1 hit.
PTHR11453:SF15. PTHR11453:SF15. 1 hit.
PfamPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSPR00165. ANIONEXCHNGR.
PR01189. ANIONEXHNGR3.
PR01231. HCO3TRNSPORT.
SUPFAMSSF55804. PTrfase/Anion_transptr. 1 hit.
TIGRFAMsTIGR00834. ae. 1 hit.
PROSITEPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298807.
SOURCESearch...

Entry information

Entry nameB3A3_MOUSE
AccessionPrimary (citable) accession number: P16283
Secondary accession number(s): Q9ERP5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents