Tyrosine-protein kinase Blk - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P16277 (BLK_MOUSE)

Last modified February 9, 2010. Version 103. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase Blk
    EC=2.7.10.2
Alternative name(s):
    B lymphocyte kinase
    p55-Blk

Gene names

Name:

Blk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

May function in a signal transduction pathway that is restricted to B-lymphoid cells.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

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Ontologies

Keywords
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 499

498

Tyrosine-protein kinase Blk

PRO_0000088062

Regions

Domain

52 – 112

SH3

Domain

118 – 214

SH2

Domain

235 – 488

254

Protein kinase

Nucleotide binding

241 – 249

ATP By similarity

Sites

Active site

354

Proton acceptor By similarity

Binding site

263

ATP By similarity

Amino acid modifications

Modified residue

383

Phosphotyrosine; by autocatalysis By similarity

Lipidation

N-myristoyl glycine By similarity

Secondary structure

499

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P16277-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: DE49C21CDBB4F1E5
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FASTA

499

56,644

        10         20         30         40         50         60 
MGLLSSKRQV SEKGKGWSPV KIRTQDKAPP PLPPLVVFNH LAPPSPNQDP DEEERFVVAL 

        70         80         90        100        110        120 
FDYAAVNDRD LQVLKGEKLQ VLRSTGDWWL ARSLVTGREG YVPSNFVAPV ETLEVEKWFF 

       130        140        150        160        170        180 
RTISRKDAER QLLAPMNKAG SFLIRESESN KGAFSLSVKD ITTQGEVVKH YKIRSLDNGG 

       190        200        210        220        230        240 
YYISPRITFP TLQALVQHYS KKGDGLCQKL TLPCVNLAPK NLWAQDEWEI PRQSLKLVRK 

       250        260        270        280        290        300 
LGSGQFGEVW MGYYKNNMKV AIKTLKEGTM SPEAFLGEAN VMKTLQHERL VRLYAVVTRE 

       310        320        330        340        350        360 
PIYIVTEYMA RGCLLDFLKT DEGSRLSLPR LIDMSAQVAE GMAYIERMNS IHRDLRAANI 

       370        380        390        400        410        420 
LVSETLCCKI ADFGLARIID SEYTAQEGAK FPIKWTAPEA IHFGVFTIKA DVWSFGVLLM 

       430        440        450        460        470        480 
VIVTYGRVPY PGMSNPEVIR SLEHGYRMPC PETCPPELYN DIITECWRGR PEERPTFEFL 

       490 
QSVLEDFYTA TEGQYELQP

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References

[1]	"Specific expression of a tyrosine kinase gene, blk, in B lymphoid cells." Dymecki S.M., Niederhuber J.E., Desiderio S.V. Science 247:332-336(1990) [PubMed: 2404338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell.
[2]	"The three-dimensional solution structure of the SH2 domain from p55blk kinase." Metzler W.J., Leiting B., Pryor K., Mueller L., Farmer B.T. II Biochemistry 35:6201-6211(1996) [PubMed: 8639560] [Abstract] Cited for: STRUCTURE BY NMR OF SH2 DOMAIN.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M30903 mRNA. Translation: AAA40453.1.

IPI

IPI00555003.

PIR

A40092.

UniGene

Mm.3962

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BLJ	NMR	-	A	107-218	[»]
1BLK	NMR	-	A	107-218	[»]

SMR

P16277. Positions 57-499.

ModBase

Search...

Protein-protein interaction databases

STRING

P16277.

PTM databases

PhosphoSite

P16277.

Proteomic databases

PRIDE

P16277.

Genome annotation databases

Ensembl

ENSMUST00000014597; ENSMUSP00000014597; ENSMUSG00000014453; Mus musculus. [Genome view]

Organism-specific databases

MGI

MGI:88169. Blk.

Phylogenomic databases

eggNOG

maNOG16387.

HOGENOM

HBG755340.

HOVERGEN

P16277.

InParanoid

P16277.

Enzyme and pathway databases

BRENDA

2.7.10.2. 244.

Gene expression databases

ArrayExpress

P16277.

Bgee

P16277.

CleanEx

MM_BLK.

Genevestigator

P16277.

GermOnline

ENSMUSG00000014453. Mus musculus.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR020473. SH3_region.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
[Graphical view]

Gene3D

G3DSA:3.30.505.10. SH2. 1 hit.

PANTHER

PTHR23256. Tyr_prot_kinase. 1 hit.

Pfam

PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. False negative.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

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Entry information

Entry name

BLK_MOUSE

Accession

Primary (citable) accession number: P16277

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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