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Protein

Cytochrome c oxidase subunit 1

Gene

ctaD

Organism
Bacillus sp. (strain PS3)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper B. This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Cu2+Note: Binds 1 copper B ion per subunit.
  • hemeNote: Binds 2 heme groups per subunit.

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Iron (heme A axial ligand)Curated1
Metal bindingi249Copper BCurated1
Metal bindingi253Copper BCurated1
Metal bindingi298Copper BCurated1
Metal bindingi299Copper BCurated1
Metal bindingi384Iron (heme A3/O axial ligand)Curated1
Metal bindingi386Iron (heme A axial ligand)Curated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome aa3 subunit 1
Cytochrome c oxidase polypeptide I
Gene namesi
Name:ctaD
Synonyms:caaB, coi
OrganismiBacillus sp. (strain PS3)
Taxonomic identifieri2334 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei28 – 48HelicalSequence analysisAdd BLAST21
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Transmembranei380 – 400HelicalSequence analysisAdd BLAST21
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Transmembranei463 – 483HelicalSequence analysisAdd BLAST21
Transmembranei553 – 573HelicalSequence analysisAdd BLAST21
Transmembranei577 – 597HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001834352 – 616Cytochrome c oxidase subunit 1Add BLAST615

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki249 ↔ 2531'-histidyl-3'-tyrosine (His-Tyr)By similarity

Structurei

3D structure databases

ProteinModelPortaliP16262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR014241. Cyt_c_oxidase_su1_bac.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02891. CtaD_CoxA. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIARKKGV GAVLWDYLTT VDHKKIAHLY LISGGFFFLL GGLEALFIRI
60 70 80 90 100
QLAKPNNDFL VGGLYNEVLT MHGTTMIFLA AMPLVFAFMN AVVPLQIGAR
110 120 130 140 150
DVAFPFLNAL GFWMFFFGGL FLNCSWFLGG APDAGWTSYA SLSLDSKAHH
160 170 180 190 200
GIDFYTLGLQ ISGFGTIMGA INFLVTIINM RAPGMTFMRM PMFTWATFVT
210 220 230 240 250
SALILFAFPP LTVGLIFMMM DRLFGGNFFN PAAGGNTIIW EHLFWVFGHP
260 270 280 290 300
EVYILVLPAF GIFSEIFATF SRKRLFGYSS MVFATVLIAF LGFMVWAHHM
310 320 330 340 350
FTVGMGPIAN AIFAVATMTI AVPTGVKIFN WLFTMWGGSI KFTTPMHYAV
360 370 380 390 400
AFIPSFVMGG VTGVMLASAA ADYQYHDSYF VVAHFHYVIV GGVVFALLAG
410 420 430 440 450
THYWWPKMFG RMLNETLGKI TFWLFFIGFH LTFFIQHFLG LTGMPRRVFT
460 470 480 490 500
YLPHQGWETG NLISTIGAFF IAAATVILLI NIVVTTAKGE KVPGDAWGDG
510 520 530 540 550
RTLEWAIASP PPVYNFAQTP LVRGLDAFWL EKMEGKKELT PAEPLGDIHM
560 570 580 590 600
PNSSFLPFVI AFGLFVAAFG FTYHNDAGWG LPVAILGLLI TLGSMFLRSV
610
IDDHGFHIHK EEVLEL
Length:616
Mass (Da):68,294
Last modified:January 23, 2007 - v3
Checksum:i17DF1E3325014B96
GO

Sequence cautioni

The sequence BAA01793 differs from that shown. Reason: Frameshift at positions 520 and 540.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti448V → W in BAA01793 (PubMed:2844737).Curated1
Sequence conflicti470F → I in BAA01793 (PubMed:2844737).Curated1
Sequence conflicti506 – 508AIA → RS in BAA01793 (PubMed:2844737).Curated3
Sequence conflicti525 – 526LD → WT AA sequence (PubMed:2844737).Curated2
Sequence conflicti549Missing AA sequence (PubMed:2844737).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13955 Genomic DNA. Translation: BAA03046.1.
D11038 Genomic DNA. Translation: BAA01793.1. Frameshift.
PIRiJX0140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13955 Genomic DNA. Translation: BAA03046.1.
D11038 Genomic DNA. Translation: BAA01793.1. Frameshift.
PIRiJX0140.

3D structure databases

ProteinModelPortaliP16262.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00705.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR014241. Cyt_c_oxidase_su1_bac.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02891. CtaD_CoxA. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOX1_BACP3
AccessioniPrimary (citable) accession number: P16262
Secondary accession number(s): Q56248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Under slightly air-limited conditions, the cytochrome a3 center of the enzyme is replaced by cytochrome o.
This cytochrome c oxidase shows clear proton pump activity in addition to electron transfer across the membrane.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.