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P16262

- COX1_BACP3

UniProt

P16262 - COX1_BACP3

Protein

Cytochrome c oxidase subunit 1

Gene

ctaD

Organism
Bacillus sp. (strain PS3)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper B. This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Cofactori

    Binds 1 copper B ion per subunit.
    Binds 2 heme groups per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi72 – 721Iron (heme A axial ligand)Curated
    Metal bindingi249 – 2491Copper BCurated
    Metal bindingi253 – 2531Copper BCurated
    Metal bindingi298 – 2981Copper BCurated
    Metal bindingi299 – 2991Copper BCurated
    Metal bindingi384 – 3841Iron (heme A3/O axial ligand)Curated
    Metal bindingi386 – 3861Iron (heme A axial ligand)Curated

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. cytochrome-c oxidase activity Source: UniProtKB-EC
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: InterPro
    2. electron transport chain Source: InterPro
    3. oxidative phosphorylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00705.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome aa3 subunit 1
    Cytochrome c oxidase polypeptide I
    Gene namesi
    Name:ctaD
    Synonyms:caaB, coi
    OrganismiBacillus sp. (strain PS3)
    Taxonomic identifieri2334 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 616615Cytochrome c oxidase subunit 1PRO_0000183435Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki249 ↔ 2531'-histidyl-3'-tyrosine (His-Tyr)By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP16262.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 4821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei75 – 9521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei102 – 12221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei158 – 17821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei198 – 21821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei275 – 29521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei380 – 40021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei420 – 44021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei463 – 48321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei553 – 57321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei577 – 59721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    IPR014241. Cyt_c_oxidase_su1_bac.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    TIGRFAMsiTIGR02891. CtaD_CoxA. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16262-1 [UniParc]FASTAAdd to Basket

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    MSTIARKKGV GAVLWDYLTT VDHKKIAHLY LISGGFFFLL GGLEALFIRI    50
    QLAKPNNDFL VGGLYNEVLT MHGTTMIFLA AMPLVFAFMN AVVPLQIGAR 100
    DVAFPFLNAL GFWMFFFGGL FLNCSWFLGG APDAGWTSYA SLSLDSKAHH 150
    GIDFYTLGLQ ISGFGTIMGA INFLVTIINM RAPGMTFMRM PMFTWATFVT 200
    SALILFAFPP LTVGLIFMMM DRLFGGNFFN PAAGGNTIIW EHLFWVFGHP 250
    EVYILVLPAF GIFSEIFATF SRKRLFGYSS MVFATVLIAF LGFMVWAHHM 300
    FTVGMGPIAN AIFAVATMTI AVPTGVKIFN WLFTMWGGSI KFTTPMHYAV 350
    AFIPSFVMGG VTGVMLASAA ADYQYHDSYF VVAHFHYVIV GGVVFALLAG 400
    THYWWPKMFG RMLNETLGKI TFWLFFIGFH LTFFIQHFLG LTGMPRRVFT 450
    YLPHQGWETG NLISTIGAFF IAAATVILLI NIVVTTAKGE KVPGDAWGDG 500
    RTLEWAIASP PPVYNFAQTP LVRGLDAFWL EKMEGKKELT PAEPLGDIHM 550
    PNSSFLPFVI AFGLFVAAFG FTYHNDAGWG LPVAILGLLI TLGSMFLRSV 600
    IDDHGFHIHK EEVLEL 616
    Length:616
    Mass (Da):68,294
    Last modified:January 23, 2007 - v3
    Checksum:i17DF1E3325014B96
    GO

    Sequence cautioni

    The sequence BAA01793.1 differs from that shown. Reason: Frameshift at positions 520 and 540.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti448 – 4481V → W in BAA01793. (PubMed:2844737)Curated
    Sequence conflicti470 – 4701F → I in BAA01793. (PubMed:2844737)Curated
    Sequence conflicti506 – 5083AIA → RS in BAA01793. (PubMed:2844737)Curated
    Sequence conflicti525 – 5262LD → WT AA sequence (PubMed:2844737)Curated
    Sequence conflicti549 – 5491Missing AA sequence (PubMed:2844737)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13955 Genomic DNA. Translation: BAA03046.1.
    D11038 Genomic DNA. Translation: BAA01793.1. Frameshift.
    PIRiJX0140.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13955 Genomic DNA. Translation: BAA03046.1 .
    D11038 Genomic DNA. Translation: BAA01793.1 . Frameshift.
    PIRi JX0140.

    3D structure databases

    ProteinModelPortali P16262.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00705 .

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    IPR014241. Cyt_c_oxidase_su1_bac.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    TIGRFAMsi TIGR02891. CtaD_CoxA. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene coding for four subunits of cytochrome c oxidase from the thermophilic bacterium PS3."
      Ishizuka M., Machida K., Shimada S., Mogi A., Tsuchiya T., Ohmori T., Souma Y., Gonda M., Sone N.
      J. Biochem. 108:866-873(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31.
    2. "Nucleotide sequence of the gene coding for cytochrome oxidase subunit I from the thermophilic bacterium PS3."
      Sone N., Yokoi F., Fu T., Ohta S., Metso T., Raitio M., Saraste M.
      J. Biochem. 103:606-610(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-552, PROTEIN SEQUENCE OF 2-15.
    3. "Haem O2 can replace haem A in the active site of cytochrome c oxidase from thermophilic bacterium PS3."
      Sone N., Fujiwara Y.
      FEBS Lett. 288:154-158(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiCOX1_BACP3
    AccessioniPrimary (citable) accession number: P16262
    Secondary accession number(s): Q56248
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Under slightly air-limited conditions, the cytochrome a3 center of the enzyme is replaced by cytochrome o.
    This cytochrome c oxidase shows clear proton pump activity in addition to electron transfer across the membrane.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3