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Protein

Translocator protein

Gene

Tspo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism, but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis (By similarity). Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed:2555358).By similarity2 Publications

GO - Molecular functioni

  • androgen binding Source: RGD
  • benzodiazepine receptor activity Source: RGD

GO - Biological processi

  • adrenal gland development Source: RGD
  • aging Source: RGD
  • behavioral response to pain Source: RGD
  • cellular hypotonic response Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • cellular response to zinc ion Source: RGD
  • chloride transport Source: RGD
  • contact inhibition Source: RGD
  • establishment of protein localization to mitochondrion Source: Ensembl
  • glial cell migration Source: RGD
  • ion transport Source: RGD
  • lipid transport Source: UniProtKB-KW
  • maintenance of protein location in mitochondrion Source: Ensembl
  • negative regulation of ATP metabolic process Source: Ensembl
  • negative regulation of glial cell proliferation Source: RGD
  • negative regulation of mitophagy Source: Ensembl
  • negative regulation of nitric oxide biosynthetic process Source: RGD
  • negative regulation of protein ubiquitination Source: Ensembl
  • negative regulation of tumor necrosis factor production Source: RGD
  • peripheral nervous system axon regeneration Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of calcium ion transport Source: RGD
  • positive regulation of glial cell proliferation Source: RGD
  • positive regulation of mitochondrial depolarization Source: RGD
  • positive regulation of necrotic cell death Source: RGD
  • positive regulation of reactive oxygen species metabolic process Source: RGD
  • regulation of steroid biosynthetic process Source: RGD
  • response to axon injury Source: RGD
  • response to drug Source: RGD
  • response to manganese ion Source: RGD
  • response to pain Source: RGD
  • response to progesterone Source: RGD
  • response to testosterone Source: RGD
  • response to vitamin B1 Source: RGD
  • steroid biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-6241-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocator protein
Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name:
PBR
Gene namesi
Name:Tspo
Synonyms:Bzrp, Mbr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2228. Tspo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 54Mitochondrial intermembraneBy similarity
Transmembranei6 – 2621Helical; Name=1By similarityAdd
BLAST
Topological domaini27 – 4620CytoplasmicBy similarityAdd
BLAST
Transmembranei47 – 6721Helical; Name=2By similarityAdd
BLAST
Topological domaini68 – 7912Mitochondrial intermembraneBy similarityAdd
BLAST
Transmembranei80 – 10021Helical; Name=3By similarityAdd
BLAST
Topological domaini101 – 1055CytoplasmicBy similarity
Transmembranei106 – 12621Helical; Name=4By similarityAdd
BLAST
Topological domaini127 – 1348Mitochondrial intermembraneBy similarity
Transmembranei135 – 15521Helical; Name=5By similarityAdd
BLAST
Topological domaini156 – 16914CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial outer membrane Source: RGD
  • mitochondrion Source: RGD
  • nuclear membrane Source: Ensembl
  • postsynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2096683.
GuidetoPHARMACOLOGYi2879.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 169168Translocator proteinPRO_0000190999Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP16257.
PRIDEiP16257.

Expressioni

Tissue specificityi

Highly expressed in adrenal gland.1 Publication

Gene expression databases

GenevisibleiP16257. RN.

Interactioni

Subunit structurei

Interacts with TSPOAP1. Interacts with MOST-1. May interact with STAR.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014089.

Chemistry

BindingDBiP16257.

Family & Domainsi

Sequence similaritiesi

Belongs to the TspO/BZRP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3797. Eukaryota.
COG3476. LUCA.
GeneTreeiENSGT00390000012980.
HOGENOMiHOG000224659.
HOVERGENiHBG001296.
InParanoidiP16257.
KOiK05770.
OMAiNPPDWLF.
OrthoDBiEOG7V7687.
PhylomeDBiP16257.
TreeFamiTF342852.

Family and domain databases

InterProiIPR030164. TSPO.
IPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PTHR10057:SF5. PTHR10057:SF5. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA
60 70 80 90 100
PIWGTLYSAM GYGSYIIWKE LGGFTEEAMV PLGLYTGQLA LNWAWPPIFF
110 120 130 140 150
GARQMGWALV DLMLVSGVAT ATTLAWHRVS PPAARLLYPY LAWLAFATML
160
NYYVWRDNSG RRGGSRLTE
Length:169
Mass (Da):18,940
Last modified:August 1, 1990 - v1
Checksum:i0D750157DB5585AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05122 mRNA. Translation: AAA41862.1.
M84221 Genomic DNA. Translation: AAA41978.1.
PIRiJC1393.
RefSeqiNP_036647.1. NM_012515.2.
UniGeneiRn.1820.
Rn.229367.

Genome annotation databases

EnsembliENSRNOT00000014089; ENSRNOP00000014089; ENSRNOG00000010549.
GeneIDi24230.
KEGGirno:24230.
UCSCiRGD:2228. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05122 mRNA. Translation: AAA41862.1.
M84221 Genomic DNA. Translation: AAA41978.1.
PIRiJC1393.
RefSeqiNP_036647.1. NM_012515.2.
UniGeneiRn.1820.
Rn.229367.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014089.

Chemistry

BindingDBiP16257.
ChEMBLiCHEMBL2096683.
GuidetoPHARMACOLOGYi2879.

Proteomic databases

PaxDbiP16257.
PRIDEiP16257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014089; ENSRNOP00000014089; ENSRNOG00000010549.
GeneIDi24230.
KEGGirno:24230.
UCSCiRGD:2228. rat.

Organism-specific databases

CTDi706.
RGDi2228. Tspo.

Phylogenomic databases

eggNOGiKOG3797. Eukaryota.
COG3476. LUCA.
GeneTreeiENSGT00390000012980.
HOGENOMiHOG000224659.
HOVERGENiHBG001296.
InParanoidiP16257.
KOiK05770.
OMAiNPPDWLF.
OrthoDBiEOG7V7687.
PhylomeDBiP16257.
TreeFamiTF342852.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-6241-MONOMER.

Miscellaneous databases

PROiP16257.

Gene expression databases

GenevisibleiP16257. RN.

Family and domain databases

InterProiIPR030164. TSPO.
IPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PTHR10057:SF5. PTHR10057:SF5. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of cDNA encoding a peripheral-type benzodiazepine receptor."
    Sprengel R., Werner P., Seeburg P.H., Mukhin A.G., Santi M.R., Grayson D.R., Guidotti A., Krueger K.E.
    J. Biol. Chem. 264:20415-20421(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Adrenal gland.
  2. "Structure of the rat gene encoding the mitochondrial benzodiazepine receptor."
    Casalotti S.O., Pelaia G., Yakovlev A.G., Csikos T., Grayson D.R., Krueger K.E.
    Gene 121:377-382(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
  3. "In vivo binding of protoporphyrin IX to rat translocator protein imaged with positron emission tomography."
    Ozaki H., Zoghbi S.S., Hong J., Verma A., Pike V.W., Innis R.B., Fujita M.
    Synapse 64:649-653(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PROTOPORPHYRIN BINDING.

Entry informationi

Entry nameiTSPO_RAT
AccessioniPrimary (citable) accession number: P16257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.