P16257 (TSPO_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translocator protein

Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name=PBR

Gene names

Name:	Tspo
Synonyms:	Bzrp, Mbr

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells By similarity.
Subunit structure	Interacts with BZRAP1 By similarity. Interacts with MOST-1 By similarity. May interact with STAR By similarity.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the TspO/BZRP family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	adrenal gland development Inferred from expression pattern PubMed 9927316. Source: RGD aging Inferred from expression pattern PubMed 12002446. Source: RGD behavioral response to pain Inferred from mutant phenotype PubMed 19555675. Source: RGD cellular hypotonic response Inferred from expression pattern PubMed 18709649. Source: RGD cellular response to lipopolysaccharide Inferred from expression pattern PubMed 14678758. Source: RGD cellular response to zinc ion Inferred from expression pattern PubMed 18709649. Source: RGD chloride transport Inferred from direct assay PubMed 14726306. Source: RGD contact inhibition Inferred from direct assay PubMed 18190798. Source: RGD glial cell migration Inferred from direct assay PubMed 18190798. Source: RGD negative regulation of glial cell proliferation Inferred from direct assay PubMed 14678758. Source: RGD negative regulation of nitric oxide biosynthetic process Inferred from direct assay PubMed 14678758. Source: RGD negative regulation of tumor necrosis factor production Inferred from direct assay PubMed 14678758. Source: RGD peripheral nervous system axon regeneration Inferred from expression pattern PubMed 8977149. Source: RGD positive regulation of apoptotic process Inferred from mutant phenotype PubMed 15284300. Source: RGD positive regulation of calcium ion transport Inferred from mutant phenotype PubMed 17174393. Source: RGD positive regulation of glial cell proliferation Inferred from direct assay PubMed 18190798. Source: RGD positive regulation of mitochondrial depolarization Inferred from direct assay PubMed 15183124. Source: RGD positive regulation of necrotic cell death Inferred from mutant phenotype PubMed 15284300. Source: RGD positive regulation of reactive oxygen species metabolic process Inferred from direct assay PubMed 12437594. Source: RGD regulation of steroid biosynthetic process Inferred from mutant phenotype PubMed 9974124. Source: RGD response to drug Inferred from expression pattern PubMed 17561380. Source: RGD response to manganese ion Inferred from expression pattern PubMed 12946575. Source: RGD response to progesterone stimulus Inferred from expression pattern PubMed 8201845. Source: RGD response to testosterone stimulus Inferred from expression pattern PubMed 8201845. Source: RGD response to vitamin B1 Inferred from expression pattern PubMed 10517697. Source: RGD steroid biosynthetic process Inferred from mutant phenotype PubMed 9405411. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from direct assay PubMed 19863077. Source: RGD
Molecular_function	androgen binding Inferred from direct assay PubMed 8918981. Source: RGD benzodiazepine receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Translocator protein		PRO_0000190999
Regions
	Transmembrane	6 – 26	21	Helical; Name=1; Potential
	Transmembrane	47 – 67	21	Helical; Name=2; Potential
	Transmembrane	80 – 100	21	Helical; Name=3; Potential
	Transmembrane	106 – 126	21	Helical; Name=4; Potential
	Transmembrane	135 – 155	21	Helical; Name=5; Potential

Sequences

	Sequence		Length	Mass (Da)
	P16257 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 0D750157DB5585AF	FASTA	169	18,940
	10 20 30 40 50 60 MSQSWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA PIWGTLYSAM 70 80 90 100 110 120 GYGSYIIWKE LGGFTEEAMV PLGLYTGQLA LNWAWPPIFF GARQMGWALV DLMLVSGVAT 130 140 150 160 ATTLAWHRVS PPAARLLYPY LAWLAFATML NYYVWRDNSG RRGGSRLTE

References

[1]	"Molecular cloning and expression of cDNA encoding a peripheral-type benzodiazepine receptor." Sprengel R., Werner P., Seeburg P.H., Mukhin A.G., Santi M.R., Grayson D.R., Guidotti A., Krueger K.E. J. Biol. Chem. 264:20415-20421(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Adrenal gland.
[2]	"Structure of the rat gene encoding the mitochondrial benzodiazepine receptor." Casalotti S.O., Pelaia G., Yakovlev A.G., Csikos T., Grayson D.R., Krueger K.E. Gene 121:377-382(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05122 mRNA. Translation: AAA41862.1. M84221 Genomic DNA. Translation: AAA41978.1.
IPI	IPI00211128.
PIR	JC1393.
RefSeq	NP_036647.1. NM_012515.2.
UniGene	Rn.1820. Rn.229367.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000014089.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000014089; ENSRNOP00000014089; ENSRNOG00000010549.
GeneID	24230.
KEGG	rno:24230.
UCSC	RGD:2228. rat.
Organism-specific databases
CTD	706.
RGD	2228. Tspo.
Phylogenomic databases
eggNOG	COG3476.
GeneTree	ENSGT00390000012980.
HOGENOM	HOG000224659.
HOVERGEN	HBG001296.
InParanoid	P16257.
KO	K05770.
OMA	ARQMGWA.
OrthoDB	EOG4HMJBC.
Gene expression databases
Genevestigator	P16257.
GermOnline	ENSRNOG00000010549. Rattus norvegicus.
Family and domain databases
InterPro	IPR004307. TspO_MBR. [Graphical view]
PANTHER	PTHR10057. PTHR10057. 1 hit.
Pfam	PF03073. TspO_MBR. 1 hit. [Graphical view]
PIRSF	PIRSF005859. PBR. 1 hit.
ProtoNet	Search...
Other
BindingDB	P16257.
ChEMBL	CHEMBL4552.
NextBio	602675.

Entry information

Entry name

TSPO_RAT

Accession

Primary (citable) accession number: P16257

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program