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P16241 (CF1A_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain protein CF1A
Alternative name(s):
Chorion factor 1A
Short name=CF1-A
Protein drifter
Ventral veins lacking protein
Gene names
Name:vvl
Synonyms:Cf1a
ORF Names:CG10037
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to a DNA sequence element required for the expression of the dopa decarboxylase gene (Ddc) in specific dopaminergic neurons. Could also play an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system. Ref.2 Ref.6

Subcellular location

Nucleus.

Tissue specificity

Coexpressed with acj6 in overlapping subsets of neurons in the embryonic epidermis and central nervous system. First detected in the precursor of the tracheal pits and the stomodeal invagination and later in the peripheral nervous system. Ref.1 Ref.2 Ref.6

Developmental stage

Expressed at maximal levels in early embryos (6-12 hours). Expressed at lower levels during development. Ref.2

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Caution

Was originally (Ref.1) thought to interact with acj6.

Sequence caution

The sequence CAA41341.1 differs from that shown. Reason: Frameshift at positions 9, 52, 102 and 420.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbrain development

Inferred from mutant phenotype PubMed 16326080. Source: FlyBase

brain segmentation

Inferred from mutant phenotype PubMed 16326080. Source: FlyBase

dendrite morphogenesis

Traceable author statement PubMed 12744843PubMed 15018941. Source: FlyBase

epithelial cell fate determination, open tracheal system

Traceable author statement PubMed 11063940PubMed 14570584. Source: FlyBase

motor neuron axon guidance

Inferred from mutant phenotype PubMed 15469973. Source: FlyBase

peripheral nervous system development

Inferred from mutant phenotype PubMed 12702660. Source: FlyBase

positive regulation of antimicrobial peptide biosynthetic process

Inferred from direct assay PubMed 20457811. Source: FlyBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19232522PubMed 20457811. Source: FlyBase

regulation of dendrite morphogenesis

Inferred from mutant phenotype PubMed 21303851. Source: FlyBase

   Cellular_componentnucleus

Inferred from direct assay PubMed 20457811. Source: FlyBase

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 17296495. Source: FlyBase

RNA polymerase II distal enhancer sequence-specific DNA binding

Inferred from direct assay PubMed 19232522. Source: FlyBase

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 9012536. Source: FlyBase

RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 20457811. Source: FlyBase

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 14570584. Source: FlyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427POU domain protein CF1A
PRO_0000100774

Regions

Domain212 – 28675POU-specific
DNA binding304 – 36360Homeobox
Compositional bias19 – 224Poly-Gly
Compositional bias42 – 198157His-rich
Compositional bias48 – 547Poly-Ala
Compositional bias103 – 1119Poly-Ala
Compositional bias380 – 40425His-rich

Experimental info

Sequence conflict83 – 10220SWSAL…THHLP → LMECPPSGSVDANPSYAPSA in CAA36496. Ref.6
Sequence conflict130 – 14011GMASPHAAWHA → ACLAPCRLAC in CAA36496. Ref.6
Sequence conflict1471A → G in CAA36496. Ref.6
Sequence conflict1691H → N in AAO39521. Ref.5
Sequence conflict364 – 37512PPNTL…DMMDG → AKYARRRHDGR in CAA36496. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P16241 [UniParc].

Last modified January 10, 2003. Version 5.
Checksum: 161F40CF3DBB8BF7

FASTA42745,927
        10         20         30         40         50         60 
MAATSYMTPP SGDLDMALGG GGYHTSSPRS AADAGEMKYM QHHHHHHAAA AAAAHHQLPS 

        70         80         90        100        110        120 
SPSPNGQGNG GGLGLGSGSG LGSWSALHPD PWMQTHHTHH LPAAAAVASA ADTVKQEMSH 

       130        140        150        160        170        180 
LSQQTRIQQG MASPHAAWHA PHAGHYAPTG GSPLQYHHAM NGMLHHPAHA VAAAHHQSVA 

       190        200        210        220        230        240 
PLHHTLRGES PQLHIHHHMG GGDRDAISGG EEDTPTSDDL EAFAKQFKQR RIKLGFTQAD 

       250        260        270        280        290        300 
VGLALGTLYG NVFSQTTICR FEALQLSFKN MCKLKPLLQK WLEEADSTTG SPTSIDKIAA 

       310        320        330        340        350        360 
QGRKRKKRTS IEVSVKGALE QHFHKQPKPS AQEITSLADS LQLEKEVVRV WFCNRRQKEK 

       370        380        390        400        410        420 
RMTPPNTLGG DMMDGMPPGH MHHGGYHPHH DMHGSPMGTH SHSHSPPMLS PQNMQSSAVA 


AHQLAAH 

« Hide

References

« Hide 'large scale' references
[1]"I-POU: a POU-domain protein that inhibits neuron-specific gene activation."
Treacy M.N., He X., Rosenfeld M.G.
Nature 350:577-584(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Isolation of a family of Drosophila POU domain genes expressed in early development."
Billin A.N., Cockerill K.A., Poole S.J.
Mech. Dev. 34:75-84(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: Oregon-R.
Tissue: Embryo.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[6]"Binding of a Drosophila POU-domain protein to a sequence element regulating gene expression in specific dopaminergic neurons."
Johnson W.A., Hirsh J.
Nature 343:467-470(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 83-427, FUNCTION, TISSUE SPECIFICITY.
[7]"Similar DNA recognition properties of alternatively spliced Drosophila POU factors."
Turner E.E.
Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SHOWS THAT CF1A DOES NOT ASSOCIATE WITH ACJ6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58435 mRNA. Translation: CAA41341.1. Frameshift.
M81959 mRNA. Translation: AAA28831.1.
AE014296 Genomic DNA. Translation: AAF50641.3.
BT003517 mRNA. Translation: AAO39521.1.
X52252 Genomic DNA. Translation: CAA36496.1.
PIRS19095.
RefSeqNP_523948.1. NM_079224.5.
UniGeneDm.2556.

3D structure databases

ProteinModelPortalP16241.
SMRP16241. Positions 215-364.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid64202. 7 interactions.
DIPDIP-17200N.
MINTMINT-1699542.

Proteomic databases

PaxDbP16241.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0076944; FBpp0076653; FBgn0086680.
GeneID38752.
KEGGdme:Dmel_CG10037.

Organism-specific databases

CTD38752.
FlyBaseFBgn0086680. vvl.

Phylogenomic databases

eggNOGNOG261729.
GeneTreeENSGT00730000110809.
InParanoidP16241.
KOK09365.
OrthoDBEOG7DJSMG.
PhylomeDBP16241.

Enzyme and pathway databases

SignaLinkP16241.

Gene expression databases

BgeeP16241.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi38752.
NextBio810212.

Entry information

Entry nameCF1A_DROME
AccessionPrimary (citable) accession number: P16241
Secondary accession number(s): Q24460, Q86P26, Q9VRY9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 10, 2003
Last modified: April 16, 2014
This is version 146 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase