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Protein

Corticosteroid 11-beta-dehydrogenase isozyme 1

Gene

Hsd11b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes reversibly the conversion of corticosterone to 11-dehydrocorticosterone.

Catalytic activityi

An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Active sitei179Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 63NADPBy similarityAdd BLAST27
Nucleotide bindingi88 – 89NADPBy similarity2
Nucleotide bindingi115 – 117NADPBy similarity3
Nucleotide bindingi179 – 183NADPBy similarity5
Nucleotide bindingi212 – 218NADPBy similarity7

GO - Molecular functioni

  • 11-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: UniProtKB-EC
  • 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: RGD
  • NADP binding Source: RGD
  • steroid binding Source: RGD

GO - Biological processi

  • cellular response to estradiol stimulus Source: RGD
  • glucocorticoid biosynthetic process Source: RGD
  • glucocorticoid catabolic process Source: RGD
  • phthalate metabolic process Source: RGD
  • regulation of pentose-phosphate shunt Source: RGD
  • response to nutrient levels Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.146. 5301.
SABIO-RKP16232.

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 1 (EC:1.1.1.146)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase 1
Short name:
11-DH
Short name:
11-beta-HSD1
Gene namesi
Name:Hsd11b1
Synonyms:Hsd11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2834. Hsd11b1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 20Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini21 – 288LumenalSequence analysisAdd BLAST268

GO - Cellular componenti

  • apical part of cell Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • nuclear membrane Source: RGD
  • rough endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110D → N: Slight loss of activity. 1
Mutagenesisi179Y → F or S: Complete loss of activity. 1 Publication1
Mutagenesisi183K → R: Complete loss of activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2391.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546231 – 288Corticosteroid 11-beta-dehydrogenase isozyme 1Add BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi158N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP16232.

PTM databases

iPTMnetiP16232.
PhosphoSitePlusiP16232.

Expressioni

Tissue specificityi

Liver, kidney, testis and placenta.

Developmental stagei

Expression of both isoforms is found in 1 week-old rats. Expression increases exponentially up to 4 weeks but after this time there is no further increase up to 16 weeks.

Gene expression databases

BgeeiENSRNOG00000005861.
ExpressionAtlasiP16232. baseline and differential.
GenevisibleiP16232. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-4580128.

Chemistry databases

BindingDBiP16232.

Structurei

3D structure databases

ProteinModelPortaliP16232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00780000121888.
HOGENOMiHOG000010276.
HOVERGENiHBG005481.
InParanoidiP16232.
KOiK15680.
OMAiFIDTESA.
PhylomeDBiP16232.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 11-HSD1A (identifier: P16232-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKYLLPVLV LCLGYYYSTN EEFRPEMLQG KKVIVTGASK GIGREMAYHL
60 70 80 90 100
SKMGAHVVLT ARSEEGLQKV VSRCLELGAA SAHYIAGTME DMAFAERFVV
110 120 130 140 150
EAGKLLGGLD MLILNHITQT TMSLFHDDIH SVRRSMEVNF LSYVVLSTAA
160 170 180 190 200
LPMLKQSNGS IAIISSMAGK MTQPLIASYS ASKFALDGFF STIRKEHLMT
210 220 230 240 250
KVNVSITLCV LGFIDTETAL KETSGIILSQ AAPKEECALE IIKGTVLRKD
260 270 280
EVYYDKSSWT PLLLGNPGRR IMEFLSLRSY NRDLFVSN
Length:288
Mass (Da):31,883
Last modified:October 10, 2002 - v2
Checksum:iAC445C0D92004808
GO
Isoform 11-HSD1B (identifier: P16232-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Note: Kidney-specific.
Show »
Length:262
Mass (Da):28,732
Checksum:i22616268BA4DF8C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31K → N in CAA71447 (PubMed:10906322).Curated1
Sequence conflicti235E → Q in AAA40886 (PubMed:2808402).Curated1
Sequence conflicti241Missing in AAA40886 (PubMed:2808402).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0126161 – 26Missing in isoform 11-HSD1B. CuratedAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05107 mRNA. Translation: AAA40886.1.
BC078865 mRNA. Translation: AAH78865.1.
S43333 Genomic DNA. Translation: AAB22993.1.
Y10420 Genomic DNA. Translation: CAA71447.1.
M77835 mRNA. Translation: AAA40600.1.
PIRiA34430. DXRTBH.
RefSeqiNP_058776.2. NM_017080.2. [P16232-1]
XP_006250535.1. XM_006250473.3. [P16232-1]
UniGeneiRn.231830.
Rn.888.

Genome annotation databases

EnsembliENSRNOT00000007870; ENSRNOP00000007870; ENSRNOG00000005861. [P16232-1]
GeneIDi25116.
KEGGirno:25116.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05107 mRNA. Translation: AAA40886.1.
BC078865 mRNA. Translation: AAH78865.1.
S43333 Genomic DNA. Translation: AAB22993.1.
Y10420 Genomic DNA. Translation: CAA71447.1.
M77835 mRNA. Translation: AAA40600.1.
PIRiA34430. DXRTBH.
RefSeqiNP_058776.2. NM_017080.2. [P16232-1]
XP_006250535.1. XM_006250473.3. [P16232-1]
UniGeneiRn.231830.
Rn.888.

3D structure databases

ProteinModelPortaliP16232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4580128.

Chemistry databases

BindingDBiP16232.
ChEMBLiCHEMBL2391.

PTM databases

iPTMnetiP16232.
PhosphoSitePlusiP16232.

Proteomic databases

PRIDEiP16232.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007870; ENSRNOP00000007870; ENSRNOG00000005861. [P16232-1]
GeneIDi25116.
KEGGirno:25116.

Organism-specific databases

CTDi3290.
RGDi2834. Hsd11b1.

Phylogenomic databases

GeneTreeiENSGT00780000121888.
HOGENOMiHOG000010276.
HOVERGENiHBG005481.
InParanoidiP16232.
KOiK15680.
OMAiFIDTESA.
PhylomeDBiP16232.

Enzyme and pathway databases

BRENDAi1.1.1.146. 5301.
SABIO-RKP16232.

Miscellaneous databases

PROiP16232.

Gene expression databases

BgeeiENSRNOG00000005861.
ExpressionAtlasiP16232. baseline and differential.
GenevisibleiP16232. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHI1_RAT
AccessioniPrimary (citable) accession number: P16232
Secondary accession number(s): O09170, Q6LDH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 10, 2002
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.