Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cathepsin E

Gene

Ctse

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain (By similarity).By similarity

Miscellaneous

Administration of dexamethasone results in the conversion of the proenzyme to the mature form in thymocytes.

Catalytic activityi

Similar to cathepsin D, but slightly broader specificity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98PROSITE-ProRule annotation1
Active sitei283PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: UniProtKB
  • protein autoprocessing Source: RGD
  • protein catabolic process Source: GO_Central

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.23.34 5301
ReactomeiR-RNO-2132295 MHC class II antigen presentation

Protein family/group databases

MEROPSiA01.010

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin E (EC:3.4.23.34)
Gene namesi
Name:Ctse
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2446 Ctse

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
PropeptideiPRO_000002598022 – 58Activation peptide1 PublicationAdd BLAST37
ChainiPRO_000002598159 – 398Cathepsin EAdd BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi62InterchainCurated
Glycosylationi92N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi111 ↔ 116By similarity
Disulfide bondi274 ↔ 278By similarity

Post-translational modificationi

Glycosylated. The nature of the carbohydrate chain varies between cell types. In brain microglia, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In stomach and spleen, the mature enzyme contains a high mannose-type oligosaccharide. In erythrocyte membranes, the mature enzyme contains a complex-type oligosaccharide.3 Publications

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP16228
PRIDEiP16228

Expressioni

Tissue specificityi

Expressed abundantly in lymphocytes and macrophages of the thymus and spleen, and in the M cells of the intestine. In the brain, expression is limited to reactive microglial cells, the large pyrimidial neurons in the cerebral cortex, the CA1 and CA3 pyrimidial neurons of the hippocampus, the large neurons of the neostriatum, and the Purkinje neurons of the cerebellum.5 Publications

Developmental stagei

Expression increases in all brain regions examined with age, and increases markedly in reactive microglial cells amd CA1 pyrimidial neurons following ischemic injury. In the thymus expression increased steadily up to 8 weeks of age before decreasing to a much lower level by 52 weeks. Expression levels in the spleen and stomach do not appear to vary with age.1 Publication

Gene expression databases

BgeeiENSRNOG00000006963
GenevisibleiP16228 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048353

Structurei

3D structure databases

ProteinModelPortaliP16228
SMRiP16228
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 394Peptidase A1PROSITE-ProRule annotationAdd BLAST315

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP16228
KOiK01382
OMAiKEPGQTF
OrthoDBiEOG091G0JP7
PhylomeDBiP16228
TreeFamiTF314990

Family and domain databases

Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR033145 Cathepsin_E
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PTHR13683:SF81 PTHR13683:SF81, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P16228-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPLFVLLLL LLLLDLAQAQ GVLHRVPLRR HQSLRKKLRA QGQLSDFWRS
60 70 80 90 100
HNLDMIEFSE SCNVDKGINE PLINYLDMEY FGTVSIGSPS QNFTVIFDTG
110 120 130 140 150
SSNLWVPSVY CTSPACKAHP VFHPSQSSTY MEVGNHFSIQ YGTGSLTGII
160 170 180 190 200
GADQVSVEGL TVEGQQFGES VKEPGQTFVN AEFDGILGLG YPSLAVGGVT
210 220 230 240 250
PVFDNMMAQN LVALPMFSVY LSSDPQGGSG SELTFGGYDP SHFSGSLNWI
260 270 280 290 300
PVTKQGYWQI ALDGIQVGDT VMFCSEGCQA IVDTGTSLIT GPPKKIKQLQ
310 320 330 340 350
EAIGATPMDG EYAVDCATLN MMPNVTFLIN GVSYTLSPTA YILPDLVDGM
360 370 380 390
QFCGSGFQGL DIQPPAGPLW ILGDVFIRKF YSVFDRGNNQ VGLAPAVP
Length:398
Mass (Da):43,021
Last modified:July 15, 1998 - v3
Checksum:i25F123E67C46EB5F
GO
Isoform 2 (identifier: P16228-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-344: Missing.

Show »
Length:365
Mass (Da):39,445
Checksum:i7AED1AB6B6A447D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55M → V in AAH62002 (PubMed:15489334).Curated1
Sequence conflicti79E → N AA sequence (PubMed:2105725).Curated1
Sequence conflicti83 – 84TV → SR AA sequence (PubMed:2105725).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti114P → S1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005224312 – 344Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38104 mRNA Translation: BAA07285.1
D45187 mRNA Translation: BAA08128.1
BC062002 mRNA Translation: AAH62002.1
PIRiA34657
S66465
S66466
RefSeqiNP_037070.1, NM_012938.1
XP_006249810.1, XM_006249748.2 [P16228-1]
XP_006249811.1, XM_006249749.2 [P16228-1]
XP_017454166.1, XM_017598677.1 [P16228-1]
UniGeneiRn.92738

Genome annotation databases

EnsembliENSRNOT00000009241; ENSRNOP00000009242; ENSRNOG00000006963 [P16228-2]
ENSRNOT00000048391; ENSRNOP00000048353; ENSRNOG00000006963 [P16228-1]
GeneIDi25424
KEGGirno:25424
UCSCiRGD:2446 rat [P16228-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCATE_RAT
AccessioniPrimary (citable) accession number: P16228
Secondary accession number(s): Q63701
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health