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P16218

- GUNH_CLOTH

UniProt

P16218 - GUNH_CLOTH

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Protein

Endoglucanase H

Gene

celH

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei460 – 4601Proton donorBy similarity
Active sitei565 – 5651NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: MENGO
  2. mannan endo-1,4-beta-mannosidase activity Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. substituted mannan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-1525-MONOMER.
MetaCyc:MONOMER-16422.

Protein family/group databases

CAZyiCBM11. Carbohydrate-Binding Module Family 11.
GH26. Glycoside Hydrolase Family 26.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase H (EC:3.2.1.4)
Alternative name(s):
Cellulase H
Endo-1,4-beta-glucanase H
Short name:
EgH
Gene namesi
Name:celH
Ordered Locus Names:Cthe_1472
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Add
BLAST
Chaini45 – 900856Endoglucanase HPRO_0000007854Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_1472.

Structurei

Secondary structure

1
900
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 365
Helixi43 – 5311
Beta strandi58 – 658
Helixi70 – 8213
Beta strandi86 – 927
Helixi98 – 1025
Turni103 – 1064
Helixi107 – 12014
Beta strandi124 – 1296
Beta strandi134 – 1374
Helixi150 – 16617
Beta strandi172 – 1754
Beta strandi178 – 1814
Helixi196 – 1983
Beta strandi200 – 2089
Turni214 – 2163
Helixi222 – 23312
Beta strandi236 – 2383
Beta strandi240 – 2478
Helixi254 – 26815
Beta strandi272 – 2787
Beta strandi281 – 2855
Helixi292 – 30110
Beta strandi656 – 6627
Beta strandi664 – 6674
Beta strandi671 – 6755
Beta strandi679 – 6868
Beta strandi688 – 69811
Beta strandi704 – 7107
Beta strandi721 – 7288
Beta strandi730 – 7323
Beta strandi736 – 7438
Beta strandi747 – 75812
Beta strandi765 – 7706
Helixi771 – 7733
Beta strandi788 – 7903
Beta strandi795 – 80612
Beta strandi809 – 81911

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V0AX-ray1.98A655-821[»]
2BV9X-ray1.50A26-304[»]
2BVDX-ray1.60A26-304[»]
2CIPX-ray1.40A26-304[»]
2CITX-ray1.40A26-304[»]
2LRONMR-A655-821[»]
2LRPNMR-A655-821[»]
2V3GX-ray1.20A26-305[»]
2VI0X-ray1.51A26-304[»]
ProteinModelPortaliP16218.
SMRiP16218. Positions 29-304, 655-821.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16218.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini655 – 900246CBM11Add
BLAST
Repeati833 – 856241Add
BLAST
Repeati872 – 895242Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 630586CatalyticBy similarityAdd
BLAST
Regioni833 – 895632 X 24 AA approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi631 – 65424Pro/Thr-rich (linker)Add
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 26 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2730.
KOiK01179.
OMAiGSANEIR.
OrthoDBiEOG66F040.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR005087. CBM_fam11.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03425. CBM_11. 1 hit.
PF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16218 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKRLLVSFL VLSIIVGLLS FQSLGNYNSG LKIGAWVGTQ PSESAIKSFQ
60 70 80 90 100
ELQGRKLDIV HQFINWSTDF SWVRPYADAV YNNGSILMIT WEPWEYNTVD
110 120 130 140 150
IKNGKADAYI TRMAQDMKAY GKEIWLRPLH EANGDWYPWA IGYSSRVNTN
160 170 180 190 200
ETYIAAFRHI VDIFRANGAT NVKWVFNVNC DNVGNGTSYL GHYPGDNYVD
210 220 230 240 250
YTSIDGYNWG TTQSWGSQWQ SFDQVFSRAY QALASINKPI IIAEFASAEI
260 270 280 290 300
GGNKARWITE AYNSIRTSYN KVIAAVWFHE NKETDWRINS SPEALAAYRE
310 320 330 340 350
AIGAGSSNPT PTPTWTSTPP SSSPKAVDPF EMVRKMGMGT NLGNTLEAPY
360 370 380 390 400
EGSWSKSAME YYFDDFKAAG YKNVRIPVRW DNHTMRTYPY TIDKAFLDRV
410 420 430 440 450
EQVVDWSLSR GFVTIINSHH DDWIKEDYNG NIERFEKIWE QIAERFKNKS
460 470 480 490 500
ENLLFEIMNE PFGNITDEQI DDMNSRILKI IRKTNPTRIV IIGGGYWNSY
510 520 530 540 550
NTLVNIKIPD DPYLIGTFHY YDPYEFTHKW RGTWGTQEDM DTVVRVFDFV
560 570 580 590 600
KSWSDRNNIP VYFGEFAVMA YADRTSRVKW YDFISDAALE RGFACSVWDN
610 620 630 640 650
GVFGSLDNDM AIYNRDTRTF DTEILNALFN PGTYPSYSPK PSPTPRPTKP
660 670 680 690 700
PVTPAVGEKM LDDFEGVLNW GSYSGEGAKV STKIVSGKTG NGMEVSYTGT
710 720 730 740 750
TDGYWGTVYS LPDGDWSKWL KISFDIKSVD GSANEIRFMI AEKSINGVGD
760 770 780 790 800
GEHWVYSITP DSSWKTIEIP FSSFRRRLDY QPPGQDMSGT LDLDNIDSIH
810 820 830 840 850
FMYANNKSGK FVVDNIKLIG ATSDPTPSIK HGDLNFDNAV NSTDLLMLKR
860 870 880 890 900
YILKSLELGT SEQEEKFKKA ADLNRDNKVD STDLTILKRY LLKAISEIPI
Length:900
Mass (Da):102,416
Last modified:April 1, 1990 - v1
Checksum:i973AFB1954FC246B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31903 Genomic DNA. Translation: AAA23225.1.
CP000568 Genomic DNA. Translation: ABN52701.1.
PIRiJH0157.
RefSeqiWP_011838089.1. NC_009012.1.
YP_001037894.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN52701; ABN52701; Cthe_1472.
GeneIDi4810622.
KEGGicth:Cthe_1472.
PATRICi19516761. VBICloThe47081_1560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31903 Genomic DNA. Translation: AAA23225.1 .
CP000568 Genomic DNA. Translation: ABN52701.1 .
PIRi JH0157.
RefSeqi WP_011838089.1. NC_009012.1.
YP_001037894.1. NC_009012.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1V0A X-ray 1.98 A 655-821 [» ]
2BV9 X-ray 1.50 A 26-304 [» ]
2BVD X-ray 1.60 A 26-304 [» ]
2CIP X-ray 1.40 A 26-304 [» ]
2CIT X-ray 1.40 A 26-304 [» ]
2LRO NMR - A 655-821 [» ]
2LRP NMR - A 655-821 [» ]
2V3G X-ray 1.20 A 26-305 [» ]
2VI0 X-ray 1.51 A 26-304 [» ]
ProteinModelPortali P16218.
SMRi P16218. Positions 29-304, 655-821.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_1472.

Protein family/group databases

CAZyi CBM11. Carbohydrate-Binding Module Family 11.
GH26. Glycoside Hydrolase Family 26.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN52701 ; ABN52701 ; Cthe_1472 .
GeneIDi 4810622.
KEGGi cth:Cthe_1472.
PATRICi 19516761. VBICloThe47081_1560.

Phylogenomic databases

eggNOGi COG2730.
KOi K01179.
OMAi GSANEIR.
OrthoDBi EOG66F040.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-1525-MONOMER.
MetaCyc:MONOMER-16422.

Miscellaneous databases

EvolutionaryTracei P16218.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR005087. CBM_fam11.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF03425. CBM_11. 1 hit.
PF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF63446. SSF63446. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and deletion analysis of the cellulase-encoding gene celH of Clostridium thermocellum."
    Yaguee E., Beguin P., Aubert J.-P.
    Gene 89:61-67(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiGUNH_CLOTH
AccessioniPrimary (citable) accession number: P16218
Secondary accession number(s): A3DFH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 1, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3