P16216 (GUN1_RUMAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 1
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Endoglucanase I
Short name=EG-I

Gene names

Name:

Eg I

Organism

Ruminococcus albus

Taxonomic identifier

1264 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ruminococcus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 43	43	Ref.1 Ref.2
	Chain	44 – 406	363	Endoglucanase 1		PRO_0000007871
Sites
	Active site	210	1	Proton donor By similarity
	Active site	330	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16216 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 2E0172437B14FEA8	FASTA	406	45,390
	10 20 30 40 50 60 MNSKKIGAMI AAAVLSLIVM TPAATRKIVQ RQTRNSSTAV ENSAADESET ENVPVSQTHT 70 80 90 100 110 120 NDTMTVTSAK DLVAKMTNGW NLGNTMDATA QGLGSEVSWL PLKVTTNKYM IDMLPEAGFN 130 140 150 160 170 180 VLRIPVSWGN HIIDDKYTSD PAWMDRVQEI VNYGIDNGLY VILNTHHEEW YMPKPSEKDG 190 200 210 220 230 240 DIEEIKAVWA QIADRFKGYD EHLIFEGLNE PRLRGEGAEW TGTSEAREII NEYEKAFVET 250 260 270 280 290 300 VRASGGNNGD RCLMITGYAA SSAYNNLSAI ELPEDSDKLI ISVHAYLPYS FALDTKGTDK 310 320 330 340 350 360 YDPEDTAIPE LFEHLNELFI SKGIPVIVGE FGTMNKENTE DRVKCLEDYL AAAAKYDIPC 370 380 390 400 VWWDNYARIG NGENFGLMNR ADLEWYFPDL IETFKTYAEK DPASAE

References

[1]	"Structure of a Ruminococcus albus endo-1,4-beta-glucanase gene." Ohmiya K., Kajino T., Kato A., Shimizu S. J. Bacteriol. 171:6771-6775(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 44-58. Strain: F-40.
[2]	"Modification of the properties of a Ruminococcus albus endo-1,4-beta-glucanase by gene truncation." Ohmiya K., Deguchi H., Shimizu S. J. Bacteriol. 173:636-641(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 44-70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30928 Genomic DNA. Translation: AAA26469.1.
PIR	A43722.
3D structure databases
ProteinModelPortal	P16216.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_RUMAL

Accession

Primary (citable) accession number: P16216

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program