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Protein

Uricase

Gene

Uro

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.1 Publication

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Enzyme regulationi

Repressed by 20-hydroxyecdysone.1 Publication

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (Uro)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei41Charge relay systemBy similarity1
Active sitei86Charge relay systemBy similarity1
Binding sitei214SubstrateBy similarity1
Binding sitei231SubstrateBy similarity1
Active sitei308Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • allantoin biosynthetic process Source: FlyBase
  • purine nucleobase metabolic process Source: UniProtKB-KW
  • response to ethanol Source: FlyBase
  • urate catabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:Uro
Synonyms:UO
ORF Names:CG7171
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003961. Uro.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659911 – 352UricaseAdd BLAST352

Proteomic databases

PaxDbiP16163.
PRIDEiP16163.

Expressioni

Tissue specificityi

Malpighian tubules.1 Publication

Developmental stagei

Third instar larvae and adult.1 Publication

Gene expression databases

BgeeiFBgn0003961.
ExpressionAtlasiP16163. differential.
GenevisibleiP16163. DM.

Interactioni

Protein-protein interaction databases

BioGridi60199. 13 interactors.
DIPiDIP-20028N.
IntActiP16163. 20 interactors.
MINTiMINT-786396.
STRINGi7227.FBpp0079109.

Structurei

3D structure databases

ProteinModelPortaliP16163.
SMRiP16163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 87Substrate bindingBy similarity2
Regioni279 – 280Substrate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi350 – 352Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
InParanoidiP16163.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP16163.

Family and domain databases

InterProiView protein in InterPro
IPR002042. Uricase.
IPR019842. Uricase_CS.
PfamiView protein in Pfam
PF01014. Uricase. 2 hits.
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiView protein in PROSITE
PS00366. URICASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P16163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFATPLRQPA AANHQTPKNS AGMDEHGKPY QYEITDHGYG KDAVKVLHVS
60 70 80 90 100
RNGPVHAIQE FEVGTHLKLY SKKDYYQGNN SDIVATDSQK NTVYLLAKKH
110 120 130 140 150
GIESPEKFAL LLARHFINKY SHVEEAHVHV EAYPWQRVCQ EETRTNVNGK
160 170 180 190 200
CENGVQGNCD FSSIDNRSLH NHAFIFTPTA LHYCDVVIRR TDPKQTVITG
210 220 230 240 250
IKGLRVLKTT QSSFVNFVND EFRSLPDQYD RIFSTVVDCS WEYSDTENLD
260 270 280 290 300
FLRAWQTVKN IIIRNFAGDP QVGVSSPSVQ HTLYLSERQV LDVLPQVSVI
310 320 330 340 350
SMTMPNKHYF NFDTKPFQKI APGDNNEVFI PVDKPHGTIY AQLARKNINS

HL
Length:352
Mass (Da):40,062
Last modified:June 1, 2001 - v2
Checksum:i1D0E00F8AD0FC96F
GO

Sequence cautioni

The sequence ABC86484 differs from that shown. Reason: Frameshift at position 342.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114R → K in CAA36203 (PubMed:2118989).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51940 Genomic DNA. Translation: CAA36203.1.
AE014134 Genomic DNA. Translation: AAF52555.1.
BT024422 mRNA. Translation: ABC86484.1. Sequence problems.
PIRiA35920.
RefSeqiNP_476779.1. NM_057431.3.
UniGeneiDm.23856.

Genome annotation databases

EnsemblMetazoaiFBtr0079486; FBpp0079109; FBgn0003961.
GeneIDi34060.
KEGGidme:Dmel_CG7171.

Similar proteinsi

Entry informationi

Entry nameiURIC_DROME
AccessioniPrimary (citable) accession number: P16163
Secondary accession number(s): Q29QG8, Q9VLX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 2001
Last modified: September 27, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families