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Protein

Uricase

Gene

Uro

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.1 Publication

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Enzyme regulationi

Repressed by 20-hydroxyecdysone.1 Publication

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (Uro)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei231 – 2311Charge relay systemBy similarity
Binding sitei231 – 2311SubstrateBy similarity
Active sitei280 – 2801Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

  • allantoin biosynthetic process Source: FlyBase
  • purine nucleobase metabolic process Source: UniProtKB-KW
  • response to ethanol Source: FlyBase
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:Uro
Synonyms:UO
ORF Names:CG7171
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003961. Uro.

Subcellular locationi

  • Peroxisome 1 Publication

GO - Cellular componenti

  • peroxisome Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352UricasePRO_0000165991Add
BLAST

Proteomic databases

PaxDbiP16163.
PRIDEiP16163.

Expressioni

Tissue specificityi

Malpighian tubules.1 Publication

Developmental stagei

Third instar larvae and adult.1 Publication

Gene expression databases

BgeeiFBgn0003961.
ExpressionAtlasiP16163. differential.
GenevisibleiP16163. DM.

Interactioni

Protein-protein interaction databases

BioGridi60199. 13 interactions.
DIPiDIP-20028N.
IntActiP16163. 11 interactions.
MINTiMINT-786396.
STRINGi7227.FBpp0079109.

Structurei

3D structure databases

ProteinModelPortaliP16163.
SMRiP16163. Positions 33-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 872Substrate bindingBy similarity
Regioni279 – 2802Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi350 – 3523Microbody targeting signalSequence analysis

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
InParanoidiP16163.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP16163.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFATPLRQPA AANHQTPKNS AGMDEHGKPY QYEITDHGYG KDAVKVLHVS
60 70 80 90 100
RNGPVHAIQE FEVGTHLKLY SKKDYYQGNN SDIVATDSQK NTVYLLAKKH
110 120 130 140 150
GIESPEKFAL LLARHFINKY SHVEEAHVHV EAYPWQRVCQ EETRTNVNGK
160 170 180 190 200
CENGVQGNCD FSSIDNRSLH NHAFIFTPTA LHYCDVVIRR TDPKQTVITG
210 220 230 240 250
IKGLRVLKTT QSSFVNFVND EFRSLPDQYD RIFSTVVDCS WEYSDTENLD
260 270 280 290 300
FLRAWQTVKN IIIRNFAGDP QVGVSSPSVQ HTLYLSERQV LDVLPQVSVI
310 320 330 340 350
SMTMPNKHYF NFDTKPFQKI APGDNNEVFI PVDKPHGTIY AQLARKNINS

HL
Length:352
Mass (Da):40,062
Last modified:June 1, 2001 - v2
Checksum:i1D0E00F8AD0FC96F
GO

Sequence cautioni

The sequence ABC86484 differs from that shown. Reason: Frameshift at position 342. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141R → K in CAA36203 (PubMed:2118989).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51940 Genomic DNA. Translation: CAA36203.1.
AE014134 Genomic DNA. Translation: AAF52555.1.
BT024422 mRNA. Translation: ABC86484.1. Sequence problems.
PIRiA35920.
RefSeqiNP_476779.1. NM_057431.3.
UniGeneiDm.23856.

Genome annotation databases

EnsemblMetazoaiFBtr0079486; FBpp0079109; FBgn0003961.
GeneIDi34060.
KEGGidme:Dmel_CG7171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51940 Genomic DNA. Translation: CAA36203.1.
AE014134 Genomic DNA. Translation: AAF52555.1.
BT024422 mRNA. Translation: ABC86484.1. Sequence problems.
PIRiA35920.
RefSeqiNP_476779.1. NM_057431.3.
UniGeneiDm.23856.

3D structure databases

ProteinModelPortaliP16163.
SMRiP16163. Positions 33-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60199. 13 interactions.
DIPiDIP-20028N.
IntActiP16163. 11 interactions.
MINTiMINT-786396.
STRINGi7227.FBpp0079109.

Proteomic databases

PaxDbiP16163.
PRIDEiP16163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079486; FBpp0079109; FBgn0003961.
GeneIDi34060.
KEGGidme:Dmel_CG7171.

Organism-specific databases

CTDi34060.
FlyBaseiFBgn0003961. Uro.

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
InParanoidiP16163.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP16163.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Miscellaneous databases

GenomeRNAii34060.
PROiP16163.

Gene expression databases

BgeeiFBgn0003961.
ExpressionAtlasiP16163. differential.
GenevisibleiP16163. DM.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURIC_DROME
AccessioniPrimary (citable) accession number: P16163
Secondary accession number(s): Q29QG8, Q9VLX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.