P16159 (IAC16_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-amylase/trypsin inhibitor CM16 Alternative name(s): Chloroform/methanol-soluble protein CM16
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-amylase/trypsin inhibitor. It could be involved in insect defense mechanisms.
Subunit structure	Subunit of the tetrameric inhibitor.
Subcellular location	Secreted.
Tissue specificity	Developing endosperm.
Post-translational modification	Five disulfide bonds are present Probable, which are essential for the inhibitor activity.
Miscellaneous	CM proteins would be involved in the cooking quality of pasta.
Sequence similarities	Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Alpha-amylase inhibitor Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.4
	Chain	25 – 143	119	Alpha-amylase/trypsin inhibitor CM16		PRO_0000014347

Sequences

	Sequence		Length	Mass (Da)
	P16159 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: DEB33F376D85ECCA	FASTA	143	15,782
	10 20 30 40 50 60 MASKSNCVLL LAAVLVSIFA AVAAIGNEDC TPWMSTLITP LPSCRDYVEQ QACRIETPGS 70 80 90 100 110 120 PYLAKQQCCG ELANIPQQCR CQALRYFMGP KSRPDQSGLM ELPGCPREVQ MDFVRILVTP 130 140 GYCNLTTVHN TPYCLAMEES QWS

References

[1]	"Cloning and characterization of a cDNA encoding the wheat (Triticum durum Desf.) CM16 protein." Gautier M.-F., Alary R., Joudrier P. Plant Mol. Biol. 14:313-322(1990) [PubMed: 2102817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Agathe. Tissue: Seed.
[2]	"Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect alpha-amylase." Garcia-Maroto F., Marana C., Mena M., Garcia-Olmedo F., Carbonero P. Plant Mol. Biol. 14:845-853(1990) [PubMed: 2102861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Chinese Spring. Tissue: Endosperm.
[3]	"Characterization of a cDNA clone encoding the Triticum aestivum L. CM16 protein: homology with the Triticum durum Desf. sequence." Lullien V., Alary R., Joudrier P., Gautier M.-F. Plant Mol. Biol. 16:373-374(1991) [PubMed: 1893108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Timgalen. Tissue: Seed.
[4]	"Evolutionary implications of sequential homologies among members of the trypsin / alpha-amylase inhibitor family (CM-proteins) in wheat and barley." Barber D., Sanchez-Monge R., Garcia-Olmedo F., Salcedo G., Mendez E. Biochim. Biophys. Acta 873:147-151(1986) Cited for: PROTEIN SEQUENCE OF 25-53.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16733 mRNA. Translation: CAA34709.1. X17573 mRNA. Translation: CAA35596.1. X55455 mRNA. Translation: CAA39100.1.
PIR	C25310. S13384.
UniGene	Ta.58.
3D structure databases
ProteinModelPortal	P16159.
ModBase	Search...
Protein family/group databases
Allergome	8777. Tri a CM16.
MEROPS	I06.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P16159.
Family and domain databases
InterPro	IPR006106. Allergen/soft/tryp_amyl_inhib. IPR006105. Allergen/tryp_amyl_inhib_CS. IPR016140. Bifunc_inhib/LTP/seed_store. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00808. AMLASEINHBTR.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITE	PS00426. CEREAL_TRYP_AMYL_INH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAC16_WHEAT

Accession

Primary (citable) accession number: P16159

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program