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P16154

- TOXA_PEPDI

UniProt

P16154 - TOXA_PEPDI

Protein

Toxin A

Gene

toxA

Organism
Peptoclostridium difficile (Clostridium difficile)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Only after the enteral delivery of the enterotoxin A may the characteristic disease called pseudomembranous colitis be induced.

    GO - Molecular functioni

    1. transferase activity, transferring glycosyl groups Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Enterotoxin, Toxin

    Protein family/group databases

    CAZyiGT44. Glycosyltransferase Family 44.
    MEROPSiC80.002.
    TCDBi1.C.57.1.2. the clostridial cytotoxin (cct) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toxin A
    Gene namesi
    Name:toxA
    Synonyms:tcdA
    OrganismiPeptoclostridium difficile (Clostridium difficile)
    Taxonomic identifieri1496 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 27102710Toxin APRO_0000072634Add
    BLAST

    Structurei

    Secondary structure

    1
    2710
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 127
    Helixi21 – 3414
    Helixi41 – 6121
    Helixi68 – 8821
    Beta strandi95 – 1006
    Helixi108 – 12013
    Turni121 – 1233
    Beta strandi124 – 1307
    Helixi137 – 16024
    Helixi167 – 19226
    Helixi201 – 21212
    Helixi217 – 23216
    Turni233 – 2353
    Beta strandi236 – 2383
    Turni239 – 2435
    Helixi248 – 25811
    Turni259 – 2613
    Helixi264 – 27916
    Beta strandi281 – 2844
    Turni294 – 2996
    Helixi308 – 32316
    Helixi334 – 3363
    Helixi339 – 35012
    Helixi355 – 3573
    Beta strandi366 – 3683
    Beta strandi373 – 3775
    Beta strandi380 – 3889
    Helixi393 – 41725
    Turni418 – 4203
    Helixi423 – 43513
    Turni440 – 4423
    Helixi443 – 4497
    Helixi452 – 4543
    Turni455 – 4573
    Helixi464 – 4674
    Helixi470 – 48213
    Helixi494 – 4974
    Helixi498 – 5003
    Helixi504 – 5063
    Helixi512 – 5176
    Helixi523 – 53614
    Helixi558 – 5636
    Helixi565 – 5695
    Beta strandi578 – 5858
    Helixi590 – 60213
    Helixi604 – 6063
    Beta strandi607 – 6115
    Helixi612 – 6176
    Beta strandi619 – 6235
    Beta strandi630 – 6345
    Helixi640 – 6423
    Beta strandi646 – 6538
    Helixi670 – 68415
    Turni685 – 6873
    Beta strandi691 – 70111
    Helixi709 – 7113
    Helixi713 – 72816
    Helixi734 – 7363
    Beta strandi737 – 7415
    Beta strandi746 – 7483
    Beta strandi754 – 7574
    Helixi766 – 7716
    Beta strandi778 – 7836
    Turni784 – 7874
    Beta strandi788 – 7958
    Beta strandi2395 – 23973
    Beta strandi2399 – 24013
    Beta strandi2410 – 24123
    Beta strandi2424 – 24296
    Beta strandi2442 – 24454
    Beta strandi2448 – 24514
    Beta strandi2454 – 24574
    Beta strandi2462 – 24654
    Beta strandi2468 – 24725
    Beta strandi2474 – 24785
    Beta strandi2482 – 24865
    Beta strandi2489 – 24935
    Turni2495 – 24973
    Beta strandi2503 – 25075
    Beta strandi2510 – 25145
    Beta strandi2523 – 25275
    Beta strandi2530 – 25345
    Beta strandi2553 – 25586
    Beta strandi2561 – 25655
    Beta strandi2574 – 25785
    Beta strandi2581 – 25855
    Turni2587 – 25893
    Beta strandi2590 – 25934
    Beta strandi2595 – 25995
    Beta strandi2602 – 26076
    Beta strandi2614 – 26185
    Beta strandi2621 – 26266
    Beta strandi2644 – 26496
    Beta strandi2652 – 26565
    Beta strandi2660 – 26623
    Beta strandi2665 – 26695
    Beta strandi2672 – 26765
    Turni2678 – 26803
    Beta strandi2686 – 26916
    Beta strandi2694 – 26985

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2F6EX-ray1.85A2583-2709[»]
    2G7CX-ray2.00A/B2456-2710[»]
    2QJ6X-ray2.50A/B2387-2706[»]
    3HO6X-ray1.60A/B543-809[»]
    4DMVX-ray1.50A1-541[»]
    4DMWX-ray2.50A1-541[»]
    ProteinModelPortaliP16154.
    SMRiP16154. Positions 1-542, 1862-1980, 2254-2706.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16154.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini583 – 768186Peptidase C80Add
    BLAST
    Repeati1810 – 182920Cell wall-binding 1Add
    BLAST
    Repeati1851 – 187020Cell wall-binding 2Add
    BLAST
    Repeati1872 – 189120Cell wall-binding 3Add
    BLAST
    Repeati1923 – 194220Cell wall-binding 4Add
    BLAST
    Repeati1943 – 196220Cell wall-binding 5Add
    BLAST
    Repeati1964 – 198320Cell wall-binding 6Add
    BLAST
    Repeati1985 – 200420Cell wall-binding 7Add
    BLAST
    Repeati2006 – 202520Cell wall-binding 8Add
    BLAST
    Repeati2057 – 207620Cell wall-binding 9Add
    BLAST
    Repeati2077 – 209620Cell wall-binding 10Add
    BLAST
    Repeati2098 – 211720Cell wall-binding 11Add
    BLAST
    Repeati2119 – 213820Cell wall-binding 12Add
    BLAST
    Repeati2140 – 215920Cell wall-binding 13Add
    BLAST
    Repeati2191 – 221020Cell wall-binding 14Add
    BLAST
    Repeati2211 – 223020Cell wall-binding 15Add
    BLAST
    Repeati2232 – 225120Cell wall-binding 16Add
    BLAST
    Repeati2252 – 227120Cell wall-binding 17Add
    BLAST
    Repeati2305 – 232420Cell wall-binding 18Add
    BLAST
    Repeati2325 – 234420Cell wall-binding 19Add
    BLAST
    Repeati2346 – 236520Cell wall-binding 20Add
    BLAST
    Repeati2367 – 238620Cell wall-binding 21Add
    BLAST
    Repeati2388 – 240720Cell wall-binding 22Add
    BLAST
    Repeati2439 – 245820Cell wall-binding 23Add
    BLAST
    Repeati2459 – 247820Cell wall-binding 24Add
    BLAST
    Repeati2480 – 249920Cell wall-binding 25Add
    BLAST
    Repeati2501 – 252020Cell wall-binding 26Add
    BLAST
    Repeati2552 – 257120Cell wall-binding 27Add
    BLAST
    Repeati2572 – 259120Cell wall-binding 28Add
    BLAST
    Repeati2593 – 261220Cell wall-binding 29Add
    BLAST
    Repeati2643 – 266220Cell wall-binding 30Add
    BLAST
    Repeati2663 – 268220Cell wall-binding 31Add
    BLAST
    Repeati2685 – 270420Cell wall-binding 32Add
    BLAST

    Domaini

    The C-terminal part of toxin A consists of a 833 AA repetitive structure. This part of toxin A is composed of five different oligopeptides.

    Sequence similaritiesi

    Belongs to the peptidase C80 family.Curated
    Contains 32 cell wall-binding repeats.PROSITE-ProRule annotation
    Contains 1 peptidase C80 domain.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR018337. Cell_wall/Cho-bd_repeat.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR020974. Pept_C80_RTX.
    IPR024770. TcdA/TcdB_cat.
    IPR024772. TcdA/TcdB_N.
    IPR024769. TcdA/TcdB_pore_forming.
    [Graphical view]
    PfamiPF01473. CW_binding_1. 12 hits.
    PF11713. Peptidase_C80. 1 hit.
    PF12919. TcdA_TcdB. 1 hit.
    PF12920. TcdA_TcdB_pore. 1 hit.
    PF12918. TcdB_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    PROSITEiPS51170. CW. 32 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16154-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLISKEELI KLAYSIRPRE NEYKTILTNL DEYNKLTTNN NENKYLQLKK     50
    LNESIDVFMN KYKTSSRNRA LSNLKKDILK EVILIKNSNT SPVEKNLHFV 100
    WIGGEVSDIA LEYIKQWADI NAEYNIKLWY DSEAFLVNTL KKAIVESSTT 150
    EALQLLEEEI QNPQFDNMKF YKKRMEFIYD RQKRFINYYK SQINKPTVPT 200
    IDDIIKSHLV SEYNRDETVL ESYRTNSLRK INSNHGIDIR ANSLFTEQEL 250
    LNIYSQELLN RGNLAAASDI VRLLALKNFG GVYLDVDMLP GIHSDLFKTI 300
    SRPSSIGLDR WEMIKLEAIM KYKKYINNYT SENFDKLDQQ LKDNFKLIIE 350
    SKSEKSEIFS KLENLNVSDL EIKIAFALGS VINQALISKQ GSYLTNLVIE 400
    QVKNRYQFLN QHLNPAIESD NNFTDTTKIF HDSLFNSATA ENSMFLTKIA 450
    PYLQVGFMPE ARSTISLSGP GAYASAYYDF INLQENTIEK TLKASDLIEF 500
    KFPENNLSQL TEQEINSLWS FDQASAKYQF EKYVRDYTGG SLSEDNGVDF 550
    NKNTALDKNY LLNNKIPSNN VEEAGSKNYV HYIIQLQGDD ISYEATCNLF 600
    SKNPKNSIII QRNMNESAKS YFLSDDGESI LELNKYRIPE RLKNKEKVKV 650
    TFIGHGKDEF NTSEFARLSV DSLSNEISSF LDTIKLDISP KNVEVNLLGC 700
    NMFSYDFNVE ETYPGKLLLS IMDKITSTLP DVNKNSITIG ANQYEVRINS 750
    EGRKELLAHS GKWINKEEAI MSDLSSKEYI FFDSIDNKLK AKSKNIPGLA 800
    SISEDIKTLL LDASVSPDTK FILNNLKLNI ESSIGDYIYY EKLEPVKNII 850
    HNSIDDLIDE FNLLENVSDE LYELKKLNNL DEKYLISFED ISKNNSTYSV 900
    RFINKSNGES VYVETEKEIF SKYSEHITKE ISTIKNSIIT DVNGNLLDNI 950
    QLDHTSQVNT LNAAFFIQSL IDYSSNKDVL NDLSTSVKVQ LYAQLFSTGL 1000
    NTIYDSIQLV NLISNAVNDT INVLPTITEG IPIVSTILDG INLGAAIKEL 1050
    LDEHDPLLKK ELEAKVGVLA INMSLSIAAT VASIVGIGAE VTIFLLPIAG 1100
    ISAGIPSLVN NELILHDKAT SVVNYFNHLS ESKKYGPLKT EDDKILVPID 1150
    DLVISEIDFN NNSIKLGTCN ILAMEGGSGH TVTGNIDHFF SSPSISSHIP 1200
    SLSIYSAIGI ETENLDFSKK IMMLPNAPSR VFWWETGAVP GLRSLENDGT 1250
    RLLDSIRDLY PGKFYWRFYA FFDYAITTLK PVYEDTNIKI KLDKDTRNFI 1300
    MPTITTNEIR NKLSYSFDGA GGTYSLLLSS YPISTNINLS KDDLWIFNID 1350
    NEVREISIEN GTIKKGKLIK DVLSKIDINK NKLIIGNQTI DFSGDIDNKD 1400
    RYIFLTCELD DKISLIIEIN LVAKSYSLLL SGDKNYLISN LSNTIEKINT 1450
    LGLDSKNIAY NYTDESNNKY FGAISKTSQK SIIHYKKDSK NILEFYNDST 1500
    LEFNSKDFIA EDINVFMKDD INTITGKYYV DNNTDKSIDF SISLVSKNQV 1550
    KVNGLYLNES VYSSYLDFVK NSDGHHNTSN FMNLFLDNIS FWKLFGFENI 1600
    NFVIDKYFTL VGKTNLGYVE FICDNNKNID IYFGEWKTSS SKSTIFSGNG 1650
    RNVVVEPIYN PDTGEDISTS LDFSYEPLYG IDRYINKVLI APDLYTSLIN 1700
    INTNYYSNEY YPEIIVLNPN TFHKKVNINL DSSSFEYKWS TEGSDFILVR 1750
    YLEESNKKIL QKIRIKGILS NTQSFNKMSI DFKDIKKLSL GYIMSNFKSF 1800
    NSENELDRDH LGFKIIDNKT YYYDEDSKLV KGLININNSL FYFDPIEFNL 1850
    VTGWQTINGK KYYFDINTGA ALTSYKIING KHFYFNNDGV MQLGVFKGPD 1900
    GFEYFAPANT QNNNIEGQAI VYQSKFLTLN GKKYYFDNNS KAVTGWRIIN 1950
    NEKYYFNPNN AIAAVGLQVI DNNKYYFNPD TAIISKGWQT VNGSRYYFDT 2000
    DTAIAFNGYK TIDGKHFYFD SDCVVKIGVF STSNGFEYFA PANTYNNNIE 2050
    GQAIVYQSKF LTLNGKKYYF DNNSKAVTGL QTIDSKKYYF NTNTAEAATG 2100
    WQTIDGKKYY FNTNTAEAAT GWQTIDGKKY YFNTNTAIAS TGYTIINGKH 2150
    FYFNTDGIMQ IGVFKGPNGF EYFAPANTDA NNIEGQAILY QNEFLTLNGK 2200
    KYYFGSDSKA VTGWRIINNK KYYFNPNNAI AAIHLCTINN DKYYFSYDGI 2250
    LQNGYITIER NNFYFDANNE SKMVTGVFKG PNGFEYFAPA NTHNNNIEGQ 2300
    AIVYQNKFLT LNGKKYYFDN DSKAVTGWQT IDGKKYYFNL NTAEAATGWQ 2350
    TIDGKKYYFN LNTAEAATGW QTIDGKKYYF NTNTFIASTG YTSINGKHFY 2400
    FNTDGIMQIG VFKGPNGFEY FAPANTDANN IEGQAILYQN KFLTLNGKKY 2450
    YFGSDSKAVT GLRTIDGKKY YFNTNTAVAV TGWQTINGKK YYFNTNTSIA 2500
    STGYTIISGK HFYFNTDGIM QIGVFKGPDG FEYFAPANTD ANNIEGQAIR 2550
    YQNRFLYLHD NIYYFGNNSK AATGWVTIDG NRYYFEPNTA MGANGYKTID 2600
    NKNFYFRNGL PQIGVFKGSN GFEYFAPANT DANNIEGQAI RYQNRFLHLL 2650
    GKIYYFGNNS KAVTGWQTIN GKVYYFMPDT AMAAAGGLFE IDGVIYFFGV 2700
    DGVKAPGIYG 2710
    Length:2,710
    Mass (Da):308,056
    Last modified:February 1, 1996 - v2
    Checksum:i0A6E52CE84C14421
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51797 Genomic DNA. Translation: CAA36094.1.
    M30307 Genomic DNA. Translation: AAA23283.1.
    X92982 Genomic DNA. Translation: CAA63564.1.
    PIRiA37052.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51797 Genomic DNA. Translation: CAA36094.1 .
    M30307 Genomic DNA. Translation: AAA23283.1 .
    X92982 Genomic DNA. Translation: CAA63564.1 .
    PIRi A37052.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2F6E X-ray 1.85 A 2583-2709 [» ]
    2G7C X-ray 2.00 A/B 2456-2710 [» ]
    2QJ6 X-ray 2.50 A/B 2387-2706 [» ]
    3HO6 X-ray 1.60 A/B 543-809 [» ]
    4DMV X-ray 1.50 A 1-541 [» ]
    4DMW X-ray 2.50 A 1-541 [» ]
    ProteinModelPortali P16154.
    SMRi P16154. Positions 1-542, 1862-1980, 2254-2706.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT44. Glycosyltransferase Family 44.
    MEROPSi C80.002.
    TCDBi 1.C.57.1.2. the clostridial cytotoxin (cct) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P16154.

    Family and domain databases

    InterProi IPR018337. Cell_wall/Cho-bd_repeat.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR020974. Pept_C80_RTX.
    IPR024770. TcdA/TcdB_cat.
    IPR024772. TcdA/TcdB_N.
    IPR024769. TcdA/TcdB_pore_forming.
    [Graphical view ]
    Pfami PF01473. CW_binding_1. 12 hits.
    PF11713. Peptidase_C80. 1 hit.
    PF12919. TcdA_TcdB. 1 hit.
    PF12920. TcdA_TcdB_pore. 1 hit.
    PF12918. TcdB_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    PROSITEi PS51170. CW. 32 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of Clostridium difficile toxin A."
      Sauerborn M., von Eichel-Streiber C.
      Nucleic Acids Res. 18:1629-1630(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 4325 / VPI 10463.
    2. "Molecular characterization of the Clostridium difficile toxin A gene."
      Dove C.H., Wang S.-Z., Price S.B., Phelps C.J., Lyerly D.M., Wilkins T.D., Johnson J.L.
      Infect. Immun. 58:480-488(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 4325 / VPI 10463.
    3. von Eichel-Streiber C.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 4325 / VPI 10463.

    Entry informationi

    Entry nameiTOXA_PEPDI
    AccessioniPrimary (citable) accession number: P16154
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3