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P16154 (TOXA_CLODI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toxin A
Gene names
Name:toxA
Synonyms:tcdA
OrganismClostridium difficile
Taxonomic identifier1496 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceae

Protein attributes

Sequence length2710 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Only after the enteral delivery of the enterotoxin A may the characteristic disease called pseudomembranous colitis be induced.

Domain

The C-terminal part of toxin A consists of a 833 AA repetitive structure. This part of toxin A is composed of five different oligopeptides.

Sequence similarities

Belongs to the peptidase C80 family.

Contains 32 cell wall-binding repeats.

Contains 1 peptidase C80 domain.

Ontologies

Keywords
   DomainRepeat
   Molecular functionEnterotoxin
Toxin
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiontransferase activity, transferring glycosyl groups

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 27102710Toxin A
PRO_0000072634

Regions

Domain583 – 768186Peptidase C80
Repeat1810 – 182920Cell wall-binding 1
Repeat1851 – 187020Cell wall-binding 2
Repeat1872 – 189120Cell wall-binding 3
Repeat1923 – 194220Cell wall-binding 4
Repeat1943 – 196220Cell wall-binding 5
Repeat1964 – 198320Cell wall-binding 6
Repeat1985 – 200420Cell wall-binding 7
Repeat2006 – 202520Cell wall-binding 8
Repeat2057 – 207620Cell wall-binding 9
Repeat2077 – 209620Cell wall-binding 10
Repeat2098 – 211720Cell wall-binding 11
Repeat2119 – 213820Cell wall-binding 12
Repeat2140 – 215920Cell wall-binding 13
Repeat2191 – 221020Cell wall-binding 14
Repeat2211 – 223020Cell wall-binding 15
Repeat2232 – 225120Cell wall-binding 16
Repeat2252 – 227120Cell wall-binding 17
Repeat2305 – 232420Cell wall-binding 18
Repeat2325 – 234420Cell wall-binding 19
Repeat2346 – 236520Cell wall-binding 20
Repeat2367 – 238620Cell wall-binding 21
Repeat2388 – 240720Cell wall-binding 22
Repeat2439 – 245820Cell wall-binding 23
Repeat2459 – 247820Cell wall-binding 24
Repeat2480 – 249920Cell wall-binding 25
Repeat2501 – 252020Cell wall-binding 26
Repeat2552 – 257120Cell wall-binding 27
Repeat2572 – 259120Cell wall-binding 28
Repeat2593 – 261220Cell wall-binding 29
Repeat2643 – 266220Cell wall-binding 30
Repeat2663 – 268220Cell wall-binding 31
Repeat2685 – 270420Cell wall-binding 32

Secondary structure

........................................................................................................................................................................................ 2710
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16154 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 0A6E52CE84C14421

FASTA2,710308,056
        10         20         30         40         50         60 
MSLISKEELI KLAYSIRPRE NEYKTILTNL DEYNKLTTNN NENKYLQLKK LNESIDVFMN 

        70         80         90        100        110        120 
KYKTSSRNRA LSNLKKDILK EVILIKNSNT SPVEKNLHFV WIGGEVSDIA LEYIKQWADI 

       130        140        150        160        170        180 
NAEYNIKLWY DSEAFLVNTL KKAIVESSTT EALQLLEEEI QNPQFDNMKF YKKRMEFIYD 

       190        200        210        220        230        240 
RQKRFINYYK SQINKPTVPT IDDIIKSHLV SEYNRDETVL ESYRTNSLRK INSNHGIDIR 

       250        260        270        280        290        300 
ANSLFTEQEL LNIYSQELLN RGNLAAASDI VRLLALKNFG GVYLDVDMLP GIHSDLFKTI 

       310        320        330        340        350        360 
SRPSSIGLDR WEMIKLEAIM KYKKYINNYT SENFDKLDQQ LKDNFKLIIE SKSEKSEIFS 

       370        380        390        400        410        420 
KLENLNVSDL EIKIAFALGS VINQALISKQ GSYLTNLVIE QVKNRYQFLN QHLNPAIESD 

       430        440        450        460        470        480 
NNFTDTTKIF HDSLFNSATA ENSMFLTKIA PYLQVGFMPE ARSTISLSGP GAYASAYYDF 

       490        500        510        520        530        540 
INLQENTIEK TLKASDLIEF KFPENNLSQL TEQEINSLWS FDQASAKYQF EKYVRDYTGG 

       550        560        570        580        590        600 
SLSEDNGVDF NKNTALDKNY LLNNKIPSNN VEEAGSKNYV HYIIQLQGDD ISYEATCNLF 

       610        620        630        640        650        660 
SKNPKNSIII QRNMNESAKS YFLSDDGESI LELNKYRIPE RLKNKEKVKV TFIGHGKDEF 

       670        680        690        700        710        720 
NTSEFARLSV DSLSNEISSF LDTIKLDISP KNVEVNLLGC NMFSYDFNVE ETYPGKLLLS 

       730        740        750        760        770        780 
IMDKITSTLP DVNKNSITIG ANQYEVRINS EGRKELLAHS GKWINKEEAI MSDLSSKEYI 

       790        800        810        820        830        840 
FFDSIDNKLK AKSKNIPGLA SISEDIKTLL LDASVSPDTK FILNNLKLNI ESSIGDYIYY 

       850        860        870        880        890        900 
EKLEPVKNII HNSIDDLIDE FNLLENVSDE LYELKKLNNL DEKYLISFED ISKNNSTYSV 

       910        920        930        940        950        960 
RFINKSNGES VYVETEKEIF SKYSEHITKE ISTIKNSIIT DVNGNLLDNI QLDHTSQVNT 

       970        980        990       1000       1010       1020 
LNAAFFIQSL IDYSSNKDVL NDLSTSVKVQ LYAQLFSTGL NTIYDSIQLV NLISNAVNDT 

      1030       1040       1050       1060       1070       1080 
INVLPTITEG IPIVSTILDG INLGAAIKEL LDEHDPLLKK ELEAKVGVLA INMSLSIAAT 

      1090       1100       1110       1120       1130       1140 
VASIVGIGAE VTIFLLPIAG ISAGIPSLVN NELILHDKAT SVVNYFNHLS ESKKYGPLKT 

      1150       1160       1170       1180       1190       1200 
EDDKILVPID DLVISEIDFN NNSIKLGTCN ILAMEGGSGH TVTGNIDHFF SSPSISSHIP 

      1210       1220       1230       1240       1250       1260 
SLSIYSAIGI ETENLDFSKK IMMLPNAPSR VFWWETGAVP GLRSLENDGT RLLDSIRDLY 

      1270       1280       1290       1300       1310       1320 
PGKFYWRFYA FFDYAITTLK PVYEDTNIKI KLDKDTRNFI MPTITTNEIR NKLSYSFDGA 

      1330       1340       1350       1360       1370       1380 
GGTYSLLLSS YPISTNINLS KDDLWIFNID NEVREISIEN GTIKKGKLIK DVLSKIDINK 

      1390       1400       1410       1420       1430       1440 
NKLIIGNQTI DFSGDIDNKD RYIFLTCELD DKISLIIEIN LVAKSYSLLL SGDKNYLISN 

      1450       1460       1470       1480       1490       1500 
LSNTIEKINT LGLDSKNIAY NYTDESNNKY FGAISKTSQK SIIHYKKDSK NILEFYNDST 

      1510       1520       1530       1540       1550       1560 
LEFNSKDFIA EDINVFMKDD INTITGKYYV DNNTDKSIDF SISLVSKNQV KVNGLYLNES 

      1570       1580       1590       1600       1610       1620 
VYSSYLDFVK NSDGHHNTSN FMNLFLDNIS FWKLFGFENI NFVIDKYFTL VGKTNLGYVE 

      1630       1640       1650       1660       1670       1680 
FICDNNKNID IYFGEWKTSS SKSTIFSGNG RNVVVEPIYN PDTGEDISTS LDFSYEPLYG 

      1690       1700       1710       1720       1730       1740 
IDRYINKVLI APDLYTSLIN INTNYYSNEY YPEIIVLNPN TFHKKVNINL DSSSFEYKWS 

      1750       1760       1770       1780       1790       1800 
TEGSDFILVR YLEESNKKIL QKIRIKGILS NTQSFNKMSI DFKDIKKLSL GYIMSNFKSF 

      1810       1820       1830       1840       1850       1860 
NSENELDRDH LGFKIIDNKT YYYDEDSKLV KGLININNSL FYFDPIEFNL VTGWQTINGK 

      1870       1880       1890       1900       1910       1920 
KYYFDINTGA ALTSYKIING KHFYFNNDGV MQLGVFKGPD GFEYFAPANT QNNNIEGQAI 

      1930       1940       1950       1960       1970       1980 
VYQSKFLTLN GKKYYFDNNS KAVTGWRIIN NEKYYFNPNN AIAAVGLQVI DNNKYYFNPD 

      1990       2000       2010       2020       2030       2040 
TAIISKGWQT VNGSRYYFDT DTAIAFNGYK TIDGKHFYFD SDCVVKIGVF STSNGFEYFA 

      2050       2060       2070       2080       2090       2100 
PANTYNNNIE GQAIVYQSKF LTLNGKKYYF DNNSKAVTGL QTIDSKKYYF NTNTAEAATG 

      2110       2120       2130       2140       2150       2160 
WQTIDGKKYY FNTNTAEAAT GWQTIDGKKY YFNTNTAIAS TGYTIINGKH FYFNTDGIMQ 

      2170       2180       2190       2200       2210       2220 
IGVFKGPNGF EYFAPANTDA NNIEGQAILY QNEFLTLNGK KYYFGSDSKA VTGWRIINNK 

      2230       2240       2250       2260       2270       2280 
KYYFNPNNAI AAIHLCTINN DKYYFSYDGI LQNGYITIER NNFYFDANNE SKMVTGVFKG 

      2290       2300       2310       2320       2330       2340 
PNGFEYFAPA NTHNNNIEGQ AIVYQNKFLT LNGKKYYFDN DSKAVTGWQT IDGKKYYFNL 

      2350       2360       2370       2380       2390       2400 
NTAEAATGWQ TIDGKKYYFN LNTAEAATGW QTIDGKKYYF NTNTFIASTG YTSINGKHFY 

      2410       2420       2430       2440       2450       2460 
FNTDGIMQIG VFKGPNGFEY FAPANTDANN IEGQAILYQN KFLTLNGKKY YFGSDSKAVT 

      2470       2480       2490       2500       2510       2520 
GLRTIDGKKY YFNTNTAVAV TGWQTINGKK YYFNTNTSIA STGYTIISGK HFYFNTDGIM 

      2530       2540       2550       2560       2570       2580 
QIGVFKGPDG FEYFAPANTD ANNIEGQAIR YQNRFLYLHD NIYYFGNNSK AATGWVTIDG 

      2590       2600       2610       2620       2630       2640 
NRYYFEPNTA MGANGYKTID NKNFYFRNGL PQIGVFKGSN GFEYFAPANT DANNIEGQAI 

      2650       2660       2670       2680       2690       2700 
RYQNRFLHLL GKIYYFGNNS KAVTGWQTIN GKVYYFMPDT AMAAAGGLFE IDGVIYFFGV 

      2710 
DGVKAPGIYG 

« Hide

References

[1]"Nucleotide sequence of Clostridium difficile toxin A."
Sauerborn M., von Eichel-Streiber C.
Nucleic Acids Res. 18:1629-1630(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 4325 / VPI 10463.
[2]"Molecular characterization of the Clostridium difficile toxin A gene."
Dove C.H., Wang S.-Z., Price S.B., Phelps C.J., Lyerly D.M., Wilkins T.D., Johnson J.L.
Infect. Immun. 58:480-488(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 4325 / VPI 10463.
[3]von Eichel-Streiber C.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 4325 / VPI 10463.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X51797 Genomic DNA. Translation: CAA36094.1.
M30307 Genomic DNA. Translation: AAA23283.1.
X92982 Genomic DNA. Translation: CAA63564.1.
PIRA37052.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2F6EX-ray1.85A2583-2709[»]
2G7CX-ray2.00A/B2456-2710[»]
2QJ6X-ray2.50A/B2387-2706[»]
3HO6X-ray1.60A/B543-809[»]
4DMVX-ray1.50A1-541[»]
4DMWX-ray2.50A1-541[»]
ProteinModelPortalP16154.
SMRP16154. Positions 1-542, 1862-1980, 2254-2706.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT44. Glycosyltransferase Family 44.
MEROPSC80.002.
TCDB1.C.57.1.2. the clostridial cytotoxin (cct) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. Pept_C80_RTX.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]
PfamPF01473. CW_binding_1. 12 hits.
PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]
PROSITEPS51170. CW. 32 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP16154.

Entry information

Entry nameTOXA_CLODI
AccessionPrimary (citable) accession number: P16154
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references