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Reviewed, UniProtKB/Swiss-Prot P16150 (LEUK_HUMAN)

Last modified November 24, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Leukosialin
Alternative name(s):
    Leukocyte sialoglycoprotein
    Sialophorin
    Galactoglycoprotein
      Short name=GALGP
    CD_antigen=CD43
Gene names
Name: SPN
Synonyms: CD43
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 By similarity. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response By similarity.

Subunit structure

Interacts with HIPK2 via the cytoplasmic domain By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Post-translational modification

Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. Ref.6

Involvement in disease

CD43 expression is defective on the T-cells of males with the immunodeficiency Wiskott-Aldrich syndrome. Affected males are susceptible to opportunistic infections and do not respond to polysaccharide antigens, reflecting defects in cytotoxic and helper T-cell functions.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.6
Chain20 – 400381Leukosialin
PRO_0000021588

Regions

Topological domain20 – 253234Extracellular Potential
Transmembrane254 – 27623 Potential
Topological domain277 – 400124Cytoplasmic Potential

Amino acid modifications

Modified residue2911Phosphoserine
Modified residue3411Phosphothreonine Ref.8
Modified residue3511Phosphoserine By similarity
Modified residue3551Phosphoserine Ref.7 Ref.9
Modified residue3681Phosphoserine
Glycosylation211O-linked (GalNAc...) Ref.6
Glycosylation221O-linked (GalNAc...) Ref.6
Glycosylation261O-linked (GalNAc...) Ref.6
Glycosylation281O-linked (GalNAc...) Ref.6
Glycosylation291O-linked (GalNAc...) Ref.6
Glycosylation351O-linked (GalNAc...) Ref.6
Glycosylation361O-linked (GalNAc...) Ref.6
Glycosylation371O-linked (GalNAc...) Ref.6
Glycosylation411O-linked (GalNAc...) Ref.6
Glycosylation421O-linked (GalNAc...) Ref.6
Glycosylation461O-linked (GalNAc...) Ref.6
Glycosylation471O-linked (GalNAc...) Ref.6
Glycosylation481O-linked (GalNAc...) Ref.6
Glycosylation501O-linked (GalNAc...) Ref.6
Glycosylation581O-linked (GalNAc...) Ref.6
Glycosylation691O-linked (GalNAc...) Ref.6
Glycosylation991O-linked (GalNAc...) Ref.6
Glycosylation1031O-linked (GalNAc...) Ref.6
Glycosylation1091O-linked (GalNAc...) Ref.6
Glycosylation1131O-linked (GalNAc...) Ref.6
Glycosylation1141O-linked (GalNAc...) Ref.6
Glycosylation1361O-linked (GalNAc...) Ref.6
Glycosylation1371O-linked (GalNAc...) Ref.6
Glycosylation1731O-linked (GalNAc...) Ref.6
Glycosylation1781O-linked (GalNAc...) Ref.6
Glycosylation2391N-linked (GlcNAc...) Ref.6

Natural variations

Natural variant221T → I: dbSNP rs2229653.
VAR_051091
Natural variant931T → A: dbSNP rs2229654.
VAR_051092

Sequences

Sequence LengthMass (Da)Tools
P16150-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: C9C9AB8435D5E1FE

FASTA40040,322
        10         20         30         40         50         60 
MATLLLLLGV LVVSPDALGS TTAVQTPTSG EPLVSTSEPL SSKMYTTSIT SDPKADSTGD 

        70         80         90        100        110        120 
QTSALPPSTS INEGSPLWTS IGASTGSPLP EPTTYQEVSI KMSSVPQETP HATSHPAVPI 

       130        140        150        160        170        180 
TANSLGSHTV TGGTITTNSP ETSSRTSGAP VTTAASSLET SRGTSGPPLT MATVSLETSK 

       190        200        210        220        230        240 
GTSGPPVTMA TDSLETSTGT TGPPVTMTTG SLEPSSGASG PQVSSVKLST MMSPTTSTNA 

       250        260        270        280        290        300 
STVPFRNPDE NSRGMLPVAV LVALLAVIVL VALLLLWRRR QKRRTGALVL SRGGKRNGVV 

       310        320        330        340        350        360 
DAWAGPAQVP EEGAVTVTVG GSGGDKGSGF PDGEGSSRRP TLTTFFGRRK SRQGSLAMEE 

       370        380        390        400 
LKSGSGPSLK GEEEPLVASE DGAVDAPAPD EPEGGDGAAP 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16."
Pallant A., Eskenazi A., Mattei M.-G., Fournier R.E.K., Carlsson S.R., Fukuda M., Frelinger J.G.
Proc. Natl. Acad. Sci. U.S.A. 86:1328-1332(1989) [PubMed: 2521952] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome."
Shelley C.S., Remold-O'Donnell E., Davis A.E. III, Bruns G.A.P., Rosen F.S., Carroll M.C., Whitehead D.A.S.
Proc. Natl. Acad. Sci. U.S.A. 86:2819-2823(1989) [PubMed: 2784859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins."
Shelley C.S., Remold-O'Donnell E., Rosen F.S., Whitehead D.A.S.
Biochem. J. 270:569-576(1990) [PubMed: 2241892] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes."
Kudo S., Fukuda M.
J. Biol. Chem. 266:8483-8489(1991) [PubMed: 1827122] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[6]"Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)."
Schmid K., Hediger M.A., Brossmer R., Collins J.H., Haupt H., Marti T., Offner G.D., Schaller J., Takagaki K., Walsh M.T., Schwick H.G., Rose F.S., Remold-O'Donnell E.
Proc. Natl. Acad. Sci. U.S.A. 89:663-667(1992) [PubMed: 1731338] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE OF 20-245, GLYCOSYLATION AT THR-21; THR-22; THR-26; THR-28; SER-29; SER-35; THR-36; SER-37; SER-41; SER-42; THR-46; THR-47; SER-48; THR-50; THR-58; THR-69; SER-99; SER-103; THR-109; THR-113; SER-114; THR-136; THR-137; THR-173; THR-178 AND ASN-239.
[7]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, MASS SPECTROMETRY.
Tissue: Platelet.
[8]"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.
J. Proteome Res. 7:5167-5176(2008) [PubMed: 19367720] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-341, MASS SPECTROMETRY.
Tissue: T-cell.
[9]Carrascal M., Abian J.
Submitted (JAN-2008) to UniProtKB
Cited for: PHOSPHORYLATION AT SER-355, MASS SPECTROMETRY.
Tissue: T-cell.
[10]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291 AND SER-368, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

J04168 mRNA. Translation: AAA59510.1.
J04536 mRNA. Translation: AAB59540.1.
X52075 Genomic DNA. Translation: CAA36294.1.
M61827 Genomic DNA. Translation: AAA51949.1.
BC012350 mRNA. Translation: AAH12350.1.
IPIIPI00027430.
PIRA39822.
RefSeqNP_001025459.1.
NP_003114.1.
UniGeneHs.632188

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP16150.

PTM databases

GlycoSuiteDBP16150.
PhosphoSiteP16150.

Proteomic databases

PeptideAtlasP16150.
PRIDEP16150.

Genome annotation databases

EnsemblENST00000360121; ENSP00000353238; ENSG00000197471; Homo sapiens. [Genome view]
ENST00000395389; ENSP00000378787; ENSG00000197471; Homo sapiens. [Genome view]
GeneID6693.
KEGGhsa:6693.
UCSCuc002dtm.1. human.

Organism-specific databases

CTD6693.
GeneCardsGC16P029581.
H-InvDBHIX0012925.
HGNCHGNC:11249. SPN.
HPACAB002666.
MIM182160. gene.
PharmGKBPA25057.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP16150.
HOVERGENP16150.
OMAGSLAMEE
OrthoDBEOG9DRCXR

Enzyme and pathway databases

BioCycCATTLE:614645-MON.
ReactomeREACT_604. Hemostasis.
REACT_6900. Signaling in Immune system.

Gene expression databases

ArrayExpressP16150.
BgeeP16150.
CleanExHS_SPN.
GenevestigatorP16150.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio26089.
PMAP-CutDBP16150.
SOURCESearch...

Entry information

Entry nameLEUK_HUMAN
AccessionPrimary (citable) accession number: P16150
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 24, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents