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Protein

Leukosialin

Gene

SPN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).By similarity2 Publications
CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival.By similarity

GO - Molecular functioni

  • heat shock protein binding Source: CAFA
  • Hsp70 protein binding Source: CAFA
  • transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  • cellular defense response Source: ProtInc
  • chemotaxis Source: ProtInc
  • defense response to bacterium Source: MGI
  • establishment or maintenance of cell polarity Source: ProtInc
  • immune response Source: ProtInc
  • interferon-gamma production Source: UniProtKB
  • leukocyte migration Source: Reactome
  • leukocyte tethering or rolling Source: UniProtKB
  • negative regulation of cell adhesion Source: ProtInc
  • negative regulation of T cell proliferation Source: UniProtKB
  • positive regulation of T cell migration Source: UniProtKB
  • positive regulation of tumor necrosis factor biosynthetic process Source: MGI
  • regulation of T cell migration Source: UniProtKB
  • signal transduction Source: ProtInc
  • T-helper 1 cell lineage commitment Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-210991. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukosialin
Alternative name(s):
GPL1151 Publication
Galactoglycoprotein
Short name:
GALGP
Leukocyte sialoglycoprotein
Sialophorin
CD_antigen: CD43
Cleaved into the following chain:
CD43 cytoplasmic tail1 Publication
Short name:
CD43-ct1 Publication
Short name:
CD43ct1 Publication
Gene namesi
Name:SPN
Synonyms:CD43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000197471.11.
HGNCiHGNC:11249. SPN.
MIMi182160. gene.
neXtProtiNX_P16150.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 253ExtracellularSequence analysisAdd BLAST234
Transmembranei254 – 276HelicalSequence analysisAdd BLAST23
Topological domaini277 – 400CytoplasmicSequence analysisAdd BLAST124

Keywords - Cellular componenti

Cell projection, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6693.
OpenTargetsiENSG00000197471.
PharmGKBiPA36079.

Polymorphism and mutation databases

BioMutaiSPN.
DMDMi126213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002158820 – 400LeukosialinAdd BLAST381
ChainiPRO_0000443406277 – 400CD43 cytoplasmic tailBy similarityAdd BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21O-linked (GalNAc...) threonine1 Publication1
Glycosylationi22O-linked (GalNAc...) threonine1 Publication1
Glycosylationi26O-linked (GalNAc...) threonine1 Publication1
Glycosylationi28O-linked (GalNAc...) threonine1 Publication1
Glycosylationi29O-linked (GalNAc...) serine1 Publication1
Glycosylationi35O-linked (GalNAc...) serine1 Publication1
Glycosylationi36O-linked (GalNAc...) threonine1 Publication1
Glycosylationi37O-linked (GalNAc...) serine1 Publication1
Glycosylationi41O-linked (GalNAc...) serine1 Publication1
Glycosylationi42O-linked (GalNAc...) serine1 Publication1
Glycosylationi46O-linked (GalNAc...) threonine1 Publication1
Glycosylationi47O-linked (GalNAc...) threonine1 Publication1
Glycosylationi48O-linked (GalNAc...) serine1 Publication1
Glycosylationi50O-linked (GalNAc...) threonine1 Publication1
Glycosylationi58O-linked (GalNAc...) threonine1 Publication1
Glycosylationi69O-linked (GalNAc...) threonine1 Publication1
Glycosylationi99O-linked (GalNAc...) serine1 Publication1
Glycosylationi103O-linked (GalNAc...) serine1 Publication1
Glycosylationi109O-linked (GalNAc...) threonine1 Publication1
Glycosylationi113O-linked (GalNAc...) threonine1 Publication1
Glycosylationi114O-linked (GalNAc...) serine1 Publication1
Glycosylationi136O-linked (GalNAc...) threonine1 Publication1
Glycosylationi137O-linked (GalNAc...) threonine1 Publication1
Glycosylationi173O-linked (GalNAc...) threonine1 Publication1
Glycosylationi178O-linked (GalNAc...) threonine1 Publication1
Glycosylationi239N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei291PhosphoserineCombined sources1 Publication1
Modified residuei336PhosphoserineCombined sources1
Modified residuei341PhosphothreonineCombined sources1
Modified residuei351PhosphoserineCombined sources1 Publication1
Modified residuei355Phosphoserine; by PKC/PRKCQCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei379PhosphoserineBy similarity1

Post-translational modificationi

Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.1 Publication
Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.By similarity
Has a high content of sialic acid and O-linked carbohydrate structures.
Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.2 Publications
CD43 cytoplasmic tail: Sumoylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP16150.
MaxQBiP16150.
PaxDbiP16150.
PeptideAtlasiP16150.
PRIDEiP16150.

PTM databases

iPTMnetiP16150.
PhosphoSitePlusiP16150.
UniCarbKBiP16150.

Miscellaneous databases

PMAP-CutDBiP16150.

Expressioni

Tissue specificityi

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Gene expression databases

BgeeiENSG00000197471.
CleanExiHS_SPN.
ExpressionAtlasiP16150. baseline and differential.
GenevisibleiP16150. HS.

Organism-specific databases

HPAiCAB002666.
HPA055244.

Interactioni

Subunit structurei

Interacts with HIPK2 via the cytoplasmic domain. Interacts with SIGLEC1, RDX, EZR and MSN.By similarity

GO - Molecular functioni

  • heat shock protein binding Source: CAFA
  • Hsp70 protein binding Source: CAFA

Protein-protein interaction databases

BioGridi112571. 12 interactors.
IntActiP16150. 4 interactors.
STRINGi9606.ENSP00000353238.

Structurei

3D structure databases

ProteinModelPortaliP16150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 308Required for interaction with EZR, MSN and RDX and for co-localization to microvilliBy similarityAdd BLAST31

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J161. Eukaryota.
ENOG410YXQE. LUCA.
GeneTreeiENSGT00390000017626.
HOGENOMiHOG000294199.
HOVERGENiHBG006258.
InParanoidiP16150.
KOiK06477.
OMAiTGPPVTM.
OrthoDBiEOG091G0MEV.
PhylomeDBiP16150.
TreeFamiTF337688.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLLLLGV LVVSPDALGS TTAVQTPTSG EPLVSTSEPL SSKMYTTSIT
60 70 80 90 100
SDPKADSTGD QTSALPPSTS INEGSPLWTS IGASTGSPLP EPTTYQEVSI
110 120 130 140 150
KMSSVPQETP HATSHPAVPI TANSLGSHTV TGGTITTNSP ETSSRTSGAP
160 170 180 190 200
VTTAASSLET SRGTSGPPLT MATVSLETSK GTSGPPVTMA TDSLETSTGT
210 220 230 240 250
TGPPVTMTTG SLEPSSGASG PQVSSVKLST MMSPTTSTNA STVPFRNPDE
260 270 280 290 300
NSRGMLPVAV LVALLAVIVL VALLLLWRRR QKRRTGALVL SRGGKRNGVV
310 320 330 340 350
DAWAGPAQVP EEGAVTVTVG GSGGDKGSGF PDGEGSSRRP TLTTFFGRRK
360 370 380 390 400
SRQGSLAMEE LKSGSGPSLK GEEEPLVASE DGAVDAPAPD EPEGGDGAAP
Length:400
Mass (Da):40,322
Last modified:April 1, 1990 - v1
Checksum:iC9C9AB8435D5E1FE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05109122T → I. Corresponds to variant dbSNP:rs2229653Ensembl.1
Natural variantiVAR_05109293T → A. Corresponds to variant dbSNP:rs2229654Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04168 mRNA. Translation: AAA59510.1.
J04536 mRNA. Translation: AAB59540.1.
X52075 Genomic DNA. Translation: CAA36294.1.
M61827 Genomic DNA. Translation: AAA51949.1.
AK292626 mRNA. Translation: BAF85315.1.
AK313750 mRNA. Translation: BAG36490.1.
CH471238 Genomic DNA. Translation: EAW80015.1.
BC012350 mRNA. Translation: AAH12350.1.
CCDSiCCDS10650.1.
PIRiA39822.
RefSeqiNP_001025459.1. NM_001030288.2.
NP_003114.1. NM_003123.4.
UniGeneiHs.632188.

Genome annotation databases

EnsembliENST00000360121; ENSP00000353238; ENSG00000197471.
ENST00000395389; ENSP00000378787; ENSG00000197471.
ENST00000563039; ENSP00000455266; ENSG00000197471.
GeneIDi6693.
KEGGihsa:6693.
UCSCiuc002dtm.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLEUK_HUMAN
AccessioniPrimary (citable) accession number: P16150
Secondary accession number(s): A8K9B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome