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P16150

- LEUK_HUMAN

UniProt

P16150 - LEUK_HUMAN

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Protein

Leukosialin

Gene
SPN, CD43
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 By similarity. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response By similarity.

GO - Molecular functioni

  1. protein binding Source: MTBBASE
  2. transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  1. apoptotic signaling pathway Source: Ensembl
  2. blood coagulation Source: Reactome
  3. cell surface receptor signaling pathway Source: Ensembl
  4. cellular defense response Source: ProtInc
  5. chemotaxis Source: ProtInc
  6. defense response to bacterium Source: MGI
  7. establishment or maintenance of cell polarity Source: ProtInc
  8. immune response Source: ProtInc
  9. leukocyte migration Source: Reactome
  10. negative regulation of cell adhesion Source: ProtInc
  11. negative regulation of T cell proliferation Source: Ensembl
  12. negative regulation of type IV hypersensitivity Source: Ensembl
  13. negative thymic T cell selection Source: Ensembl
  14. positive regulation of T cell proliferation Source: Ensembl
  15. positive regulation of tumor necrosis factor biosynthetic process Source: MGI
  16. regulation of defense response to virus Source: Ensembl
  17. response to protozoan Source: Ensembl
  18. signal transduction Source: ProtInc
  19. T cell costimulation Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.
REACT_12560. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukosialin
Alternative name(s):
Galactoglycoprotein
Short name:
GALGP
Leukocyte sialoglycoprotein
Sialophorin
CD_antigen: CD43
Gene namesi
Name:SPN
Synonyms:CD43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:11249. SPN.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 253234Extracellular Reviewed predictionAdd
BLAST
Transmembranei254 – 27623Helical; Reviewed predictionAdd
BLAST
Topological domaini277 – 400124Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. basement membrane Source: Ensembl
  2. cell surface Source: UniProtKB
  3. external side of plasma membrane Source: Ensembl
  4. extracellular space Source: MGI
  5. extracellular vesicular exosome Source: UniProt
  6. integral component of plasma membrane Source: ProtInc
  7. plasma membrane Source: Reactome
  8. uropod Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36079.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 400381LeukosialinPRO_0000021588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211O-linked (GalNAc...)1 Publication
Glycosylationi22 – 221O-linked (GalNAc...)1 Publication
Glycosylationi26 – 261O-linked (GalNAc...)1 Publication
Glycosylationi28 – 281O-linked (GalNAc...)1 Publication
Glycosylationi29 – 291O-linked (GalNAc...)1 Publication
Glycosylationi35 – 351O-linked (GalNAc...)1 Publication
Glycosylationi36 – 361O-linked (GalNAc...)1 Publication
Glycosylationi37 – 371O-linked (GalNAc...)1 Publication
Glycosylationi41 – 411O-linked (GalNAc...)1 Publication
Glycosylationi42 – 421O-linked (GalNAc...)1 Publication
Glycosylationi46 – 461O-linked (GalNAc...)1 Publication
Glycosylationi47 – 471O-linked (GalNAc...)1 Publication
Glycosylationi48 – 481O-linked (GalNAc...)1 Publication
Glycosylationi50 – 501O-linked (GalNAc...)1 Publication
Glycosylationi58 – 581O-linked (GalNAc...)1 Publication
Glycosylationi69 – 691O-linked (GalNAc...)1 Publication
Glycosylationi99 – 991O-linked (GalNAc...)1 Publication
Glycosylationi103 – 1031O-linked (GalNAc...)1 Publication
Glycosylationi109 – 1091O-linked (GalNAc...)1 Publication
Glycosylationi113 – 1131O-linked (GalNAc...)1 Publication
Glycosylationi114 – 1141O-linked (GalNAc...)1 Publication
Glycosylationi136 – 1361O-linked (GalNAc...)1 Publication
Glycosylationi137 – 1371O-linked (GalNAc...)1 Publication
Glycosylationi173 – 1731O-linked (GalNAc...)1 Publication
Glycosylationi178 – 1781O-linked (GalNAc...)1 Publication
Glycosylationi239 – 2391N-linked (GlcNAc...)1 Publication
Modified residuei291 – 2911Phosphoserine1 Publication
Modified residuei351 – 3511Phosphoserine1 Publication
Modified residuei355 – 3551Phosphoserine2 Publications
Modified residuei368 – 3681Phosphoserine1 Publication

Post-translational modificationi

Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP16150.
PaxDbiP16150.
PeptideAtlasiP16150.
PRIDEiP16150.

PTM databases

PhosphoSiteiP16150.
UniCarbKBiP16150.

Miscellaneous databases

PMAP-CutDBP16150.

Expressioni

Tissue specificityi

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Gene expression databases

ArrayExpressiP16150.
BgeeiP16150.
CleanExiHS_SPN.
GenevestigatoriP16150.

Organism-specific databases

HPAiCAB002666.
HPA055244.

Interactioni

Subunit structurei

Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX By similarity.

Protein-protein interaction databases

BioGridi112571. 4 interactions.
STRINGi9606.ENSP00000353238.

Structurei

3D structure databases

ProteinModelPortaliP16150.

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47487.
HOGENOMiHOG000294199.
HOVERGENiHBG006258.
InParanoidiP16150.
KOiK06477.
OMAiGSLAMEE.
OrthoDBiEOG7HXCVB.
PhylomeDBiP16150.
TreeFamiTF337688.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16150-1 [UniParc]FASTAAdd to Basket

« Hide

MATLLLLLGV LVVSPDALGS TTAVQTPTSG EPLVSTSEPL SSKMYTTSIT    50
SDPKADSTGD QTSALPPSTS INEGSPLWTS IGASTGSPLP EPTTYQEVSI 100
KMSSVPQETP HATSHPAVPI TANSLGSHTV TGGTITTNSP ETSSRTSGAP 150
VTTAASSLET SRGTSGPPLT MATVSLETSK GTSGPPVTMA TDSLETSTGT 200
TGPPVTMTTG SLEPSSGASG PQVSSVKLST MMSPTTSTNA STVPFRNPDE 250
NSRGMLPVAV LVALLAVIVL VALLLLWRRR QKRRTGALVL SRGGKRNGVV 300
DAWAGPAQVP EEGAVTVTVG GSGGDKGSGF PDGEGSSRRP TLTTFFGRRK 350
SRQGSLAMEE LKSGSGPSLK GEEEPLVASE DGAVDAPAPD EPEGGDGAAP 400
Length:400
Mass (Da):40,322
Last modified:April 1, 1990 - v1
Checksum:iC9C9AB8435D5E1FE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti22 – 221T → I.
Corresponds to variant rs2229653 [ dbSNP | Ensembl ].
VAR_051091
Natural varianti93 – 931T → A.
Corresponds to variant rs2229654 [ dbSNP | Ensembl ].
VAR_051092

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04168 mRNA. Translation: AAA59510.1.
J04536 mRNA. Translation: AAB59540.1.
X52075 Genomic DNA. Translation: CAA36294.1.
M61827 Genomic DNA. Translation: AAA51949.1.
AK292626 mRNA. Translation: BAF85315.1.
AK313750 mRNA. Translation: BAG36490.1.
CH471238 Genomic DNA. Translation: EAW80015.1.
BC012350 mRNA. Translation: AAH12350.1.
CCDSiCCDS10650.1.
PIRiA39822.
RefSeqiNP_001025459.1. NM_001030288.2.
NP_003114.1. NM_003123.4.
XP_005276570.1. XM_005276513.1.
UniGeneiHs.632188.

Genome annotation databases

EnsembliENST00000360121; ENSP00000353238; ENSG00000197471.
ENST00000395389; ENSP00000378787; ENSG00000197471.
ENST00000563039; ENSP00000455266; ENSG00000197471.
GeneIDi101929889.
6693.
KEGGihsa:101929889.
hsa:6693.
UCSCiuc002dtm.4. human.

Polymorphism databases

DMDMi126213.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04168 mRNA. Translation: AAA59510.1 .
J04536 mRNA. Translation: AAB59540.1 .
X52075 Genomic DNA. Translation: CAA36294.1 .
M61827 Genomic DNA. Translation: AAA51949.1 .
AK292626 mRNA. Translation: BAF85315.1 .
AK313750 mRNA. Translation: BAG36490.1 .
CH471238 Genomic DNA. Translation: EAW80015.1 .
BC012350 mRNA. Translation: AAH12350.1 .
CCDSi CCDS10650.1.
PIRi A39822.
RefSeqi NP_001025459.1. NM_001030288.2.
NP_003114.1. NM_003123.4.
XP_005276570.1. XM_005276513.1.
UniGenei Hs.632188.

3D structure databases

ProteinModelPortali P16150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112571. 4 interactions.
STRINGi 9606.ENSP00000353238.

PTM databases

PhosphoSitei P16150.
UniCarbKBi P16150.

Polymorphism databases

DMDMi 126213.

Proteomic databases

MaxQBi P16150.
PaxDbi P16150.
PeptideAtlasi P16150.
PRIDEi P16150.

Protocols and materials databases

DNASUi 6693.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000360121 ; ENSP00000353238 ; ENSG00000197471 .
ENST00000395389 ; ENSP00000378787 ; ENSG00000197471 .
ENST00000563039 ; ENSP00000455266 ; ENSG00000197471 .
GeneIDi 101929889.
6693.
KEGGi hsa:101929889.
hsa:6693.
UCSCi uc002dtm.4. human.

Organism-specific databases

CTDi 6693.
GeneCardsi GC16P029674.
HGNCi HGNC:11249. SPN.
HPAi CAB002666.
HPA055244.
MIMi 182160. gene.
neXtProti NX_P16150.
PharmGKBi PA36079.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG47487.
HOGENOMi HOG000294199.
HOVERGENi HBG006258.
InParanoidi P16150.
KOi K06477.
OMAi GSLAMEE.
OrthoDBi EOG7HXCVB.
PhylomeDBi P16150.
TreeFami TF337688.

Enzyme and pathway databases

Reactomei REACT_12051. Cell surface interactions at the vascular wall.
REACT_12560. Basigin interactions.

Miscellaneous databases

GeneWikii CD43.
NextBioi 26089.
PMAP-CutDB P16150.
PROi P16150.
SOURCEi Search...

Gene expression databases

ArrayExpressi P16150.
Bgeei P16150.
CleanExi HS_SPN.
Genevestigatori P16150.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16."
    Pallant A., Eskenazi A., Mattei M.-G., Fournier R.E.K., Carlsson S.R., Fukuda M., Frelinger J.G.
    Proc. Natl. Acad. Sci. U.S.A. 86:1328-1332(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome."
    Shelley C.S., Remold-O'Donnell E., Davis A.E. III, Bruns G.A.P., Rosen F.S., Carroll M.C., Whitehead D.A.S.
    Proc. Natl. Acad. Sci. U.S.A. 86:2819-2823(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins."
    Shelley C.S., Remold-O'Donnell E., Rosen F.S., Whitehead D.A.S.
    Biochem. J. 270:569-576(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes."
    Kudo S., Fukuda M.
    J. Biol. Chem. 266:8483-8489(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen and Thymus.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  8. "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)."
    Schmid K., Hediger M.A., Brossmer R., Collins J.H., Haupt H., Marti T., Offner G.D., Schaller J., Takagaki K., Walsh M.T., Schwick H.G., Rose F.S., Remold-O'Donnell E.
    Proc. Natl. Acad. Sci. U.S.A. 89:663-667(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-245, GLYCOSYLATION AT THR-21; THR-22; THR-26; THR-28; SER-29; SER-35; THR-36; SER-37; SER-41; SER-42; THR-46; THR-47; SER-48; THR-50; THR-58; THR-69; SER-99; SER-103; THR-109; THR-113; SER-114; THR-136; THR-137; THR-173; THR-178 AND ASN-239.
  9. "Phosphorylation of the major leukocyte surface sialoglycoprotein, leukosialin, is increased by phorbol 12-myristate 13-acetate."
    Piller V., Piller F., Fukuda M.
    J. Biol. Chem. 264:18824-18831(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-291 AND SER-351.
  10. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
    Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.
    J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: T-cell.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  12. Carrascal M., Abian J.
    Submitted (JAN-2008) to UniProtKB
    Cited for: PHOSPHORYLATION AT SER-355, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: T-cell.
  13. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLEUK_HUMAN
AccessioniPrimary (citable) accession number: P16150
Secondary accession number(s): A8K9B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 3, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

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