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Protein

Leukosialin

Gene

SPN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity).By similarity

GO - Molecular functioni

  • heat shock protein binding Source: CAFA
  • Hsp70 protein binding Source: CAFA
  • transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  • apoptotic signaling pathway Source: Ensembl
  • cell surface receptor signaling pathway Source: Ensembl
  • cellular defense response Source: ProtInc
  • chemotaxis Source: ProtInc
  • defense response to bacterium Source: MGI
  • establishment or maintenance of cell polarity Source: ProtInc
  • immune response Source: ProtInc
  • leukocyte migration Source: Reactome
  • negative regulation of cell adhesion Source: ProtInc
  • negative regulation of T cell proliferation Source: Ensembl
  • negative regulation of type IV hypersensitivity Source: Ensembl
  • negative thymic T cell selection Source: Ensembl
  • positive regulation of protein phosphorylation Source: Ensembl
  • positive regulation of T cell proliferation Source: Ensembl
  • positive regulation of tumor necrosis factor biosynthetic process Source: MGI
  • regulation of defense response to virus Source: Ensembl
  • response to protozoan Source: Ensembl
  • signal transduction Source: ProtInc
  • T cell costimulation Source: Ensembl

Enzyme and pathway databases

ReactomeiR-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-210991. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukosialin
Alternative name(s):
Galactoglycoprotein
Short name:
GALGP
Leukocyte sialoglycoprotein
Sialophorin
CD_antigen: CD43
Gene namesi
Name:SPN
Synonyms:CD43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000197471.11.
HGNCiHGNC:11249. SPN.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 253ExtracellularSequence analysisAdd BLAST234
Transmembranei254 – 276HelicalSequence analysisAdd BLAST23
Topological domaini277 – 400CytoplasmicSequence analysisAdd BLAST124

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6693.
OpenTargetsiENSG00000197471.
PharmGKBiPA36079.

Polymorphism and mutation databases

BioMutaiSPN.
DMDMi126213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002158820 – 400LeukosialinAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21O-linked (GalNAc...) threonine1 Publication1
Glycosylationi22O-linked (GalNAc...) threonine1 Publication1
Glycosylationi26O-linked (GalNAc...) threonine1 Publication1
Glycosylationi28O-linked (GalNAc...) threonine1 Publication1
Glycosylationi29O-linked (GalNAc...) serine1 Publication1
Glycosylationi35O-linked (GalNAc...) serine1 Publication1
Glycosylationi36O-linked (GalNAc...) threonine1 Publication1
Glycosylationi37O-linked (GalNAc...) serine1 Publication1
Glycosylationi41O-linked (GalNAc...) serine1 Publication1
Glycosylationi42O-linked (GalNAc...) serine1 Publication1
Glycosylationi46O-linked (GalNAc...) threonine1 Publication1
Glycosylationi47O-linked (GalNAc...) threonine1 Publication1
Glycosylationi48O-linked (GalNAc...) serine1 Publication1
Glycosylationi50O-linked (GalNAc...) threonine1 Publication1
Glycosylationi58O-linked (GalNAc...) threonine1 Publication1
Glycosylationi69O-linked (GalNAc...) threonine1 Publication1
Glycosylationi99O-linked (GalNAc...) serine1 Publication1
Glycosylationi103O-linked (GalNAc...) serine1 Publication1
Glycosylationi109O-linked (GalNAc...) threonine1 Publication1
Glycosylationi113O-linked (GalNAc...) threonine1 Publication1
Glycosylationi114O-linked (GalNAc...) serine1 Publication1
Glycosylationi136O-linked (GalNAc...) threonine1 Publication1
Glycosylationi137O-linked (GalNAc...) threonine1 Publication1
Glycosylationi173O-linked (GalNAc...) threonine1 Publication1
Glycosylationi178O-linked (GalNAc...) threonine1 Publication1
Glycosylationi239N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei291PhosphoserineCombined sources1 Publication1
Modified residuei336PhosphoserineCombined sources1
Modified residuei341PhosphothreonineCombined sources1
Modified residuei351PhosphoserineCombined sources1 Publication1
Modified residuei355PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei379PhosphoserineBy similarity1

Post-translational modificationi

Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP16150.
MaxQBiP16150.
PaxDbiP16150.
PeptideAtlasiP16150.
PRIDEiP16150.

PTM databases

iPTMnetiP16150.
PhosphoSitePlusiP16150.
UniCarbKBiP16150.

Miscellaneous databases

PMAP-CutDBiP16150.

Expressioni

Tissue specificityi

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Gene expression databases

BgeeiENSG00000197471.
CleanExiHS_SPN.
ExpressionAtlasiP16150. baseline and differential.
GenevisibleiP16150. HS.

Organism-specific databases

HPAiCAB002666.
HPA055244.

Interactioni

Subunit structurei

Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX (By similarity).By similarity

GO - Molecular functioni

  • heat shock protein binding Source: CAFA
  • Hsp70 protein binding Source: CAFA

Protein-protein interaction databases

BioGridi112571. 12 interactors.
IntActiP16150. 4 interactors.
STRINGi9606.ENSP00000353238.

Structurei

3D structure databases

ProteinModelPortaliP16150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J161. Eukaryota.
ENOG410YXQE. LUCA.
GeneTreeiENSGT00390000017626.
HOGENOMiHOG000294199.
HOVERGENiHBG006258.
InParanoidiP16150.
KOiK06477.
OMAiGSLAMEE.
OrthoDBiEOG091G0MEV.
PhylomeDBiP16150.
TreeFamiTF337688.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLLLLGV LVVSPDALGS TTAVQTPTSG EPLVSTSEPL SSKMYTTSIT
60 70 80 90 100
SDPKADSTGD QTSALPPSTS INEGSPLWTS IGASTGSPLP EPTTYQEVSI
110 120 130 140 150
KMSSVPQETP HATSHPAVPI TANSLGSHTV TGGTITTNSP ETSSRTSGAP
160 170 180 190 200
VTTAASSLET SRGTSGPPLT MATVSLETSK GTSGPPVTMA TDSLETSTGT
210 220 230 240 250
TGPPVTMTTG SLEPSSGASG PQVSSVKLST MMSPTTSTNA STVPFRNPDE
260 270 280 290 300
NSRGMLPVAV LVALLAVIVL VALLLLWRRR QKRRTGALVL SRGGKRNGVV
310 320 330 340 350
DAWAGPAQVP EEGAVTVTVG GSGGDKGSGF PDGEGSSRRP TLTTFFGRRK
360 370 380 390 400
SRQGSLAMEE LKSGSGPSLK GEEEPLVASE DGAVDAPAPD EPEGGDGAAP
Length:400
Mass (Da):40,322
Last modified:April 1, 1990 - v1
Checksum:iC9C9AB8435D5E1FE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05109122T → I. Corresponds to variant dbSNP:rs2229653Ensembl.1
Natural variantiVAR_05109293T → A. Corresponds to variant dbSNP:rs2229654Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04168 mRNA. Translation: AAA59510.1.
J04536 mRNA. Translation: AAB59540.1.
X52075 Genomic DNA. Translation: CAA36294.1.
M61827 Genomic DNA. Translation: AAA51949.1.
AK292626 mRNA. Translation: BAF85315.1.
AK313750 mRNA. Translation: BAG36490.1.
CH471238 Genomic DNA. Translation: EAW80015.1.
BC012350 mRNA. Translation: AAH12350.1.
CCDSiCCDS10650.1.
PIRiA39822.
RefSeqiNP_001025459.1. NM_001030288.2.
NP_003114.1. NM_003123.4.
UniGeneiHs.632188.

Genome annotation databases

EnsembliENST00000360121; ENSP00000353238; ENSG00000197471.
ENST00000395389; ENSP00000378787; ENSG00000197471.
ENST00000563039; ENSP00000455266; ENSG00000197471.
GeneIDi6693.
KEGGihsa:6693.
UCSCiuc002dtm.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLEUK_HUMAN
AccessioniPrimary (citable) accession number: P16150
Secondary accession number(s): A8K9B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 25, 2017
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot