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Protein

POU domain, class 2, transcription factor 1

Gene

pou2f1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and histone H2B. Acts downstream of Notch signaling during radial glia formation. Regulates apoptosis, possibly via an FGF-signaling pathway.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi395 – 454HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • Notch signaling pathway Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • radial glial cell differentiation Source: UniProtKB
  • regulation of apoptotic process Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
Octamer-binding protein 1
Short name:
Oct-1
Short name:
XOct1
Octamer-binding transcription factor 1
Short name:
OTF-1
Xloct1-32
Gene namesi
Name:pou2f1
Synonyms:oct-1, oct1, oct1.32
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-853997. pou2f1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Retained in the cytoplasm during early development, then gradually translocates to the nucleus around the mid-blastula transition (MBT).

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007131 – 758POU domain, class 2, transcription factor 1Add BLAST758

Proteomic databases

PRIDEiP16143.

Expressioni

Tissue specificityi

Abundant in the animal hemisphere of the blastula embryo and during early embryogenesis. Expressed in ectodermal and mesodermal cell lineages where expression becomes spatially restricted to the developing nervous system as development progresses (at protein level). Expressed in the neural plate at late neurula stage (stage 17). At early tailbud stage (stage 25), expression continues in the neural tube and begins in the eyes. At late tailbud stages (stage 30), expressed broadly in the CNS.3 Publications

Developmental stagei

Expressed both maternally and zygotically.3 Publications

Structurei

3D structure databases

ProteinModelPortaliP16143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 368POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi457 – 573Ser-richAdd BLAST117

Domaini

The POU domain controls nuclear translocation.1 Publication

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG057016.
KOiK09364.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P16143-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLHSSSKIQ NHAWLSDARM NNPSETSKSP ESGDGNTGTQ TNGLDFQKQA
60 70 80 90 100
VPIGAITSAQ AQALLGHLHQ VQLAGTSLQA AAHSLNVQTK FKEEPGEPMQ
110 120 130 140 150
VVQPSQQPSL QAAIPQTQLM VAGGQIAGLT LTPAQQQMLL QQAQAQLLAA
160 170 180 190 200
AVQHSASQQH NAAGATISAS AATPMTQIPL SQPIQIAQDL QQLQQLQQQN
210 220 230 240 250
LNLQQYVLVH PTTNLQSAQF IISQTPQGQQ GLLQAQNLLT QLPQQSQANL
260 270 280 290 300
LQSQPSITLT SQPATPTRTI AATPVQQLPQ SQTTPKRIDT PSLEEPSDLE
310 320 330 340 350
ELEQFAKTFK QRRIKLGFTQ GDVGLAMGKL YGNDFSQTTI SRFEALNLSF
360 370 380 390 400
KNMCKLKPLL EKWLNDAENI TSDSTLTNQS VLNSPGHGME GLNRRRKKRT
410 420 430 440 450
SIETNIRVAL EKSFLENQKP TSEEITMIAD QLNMEKEVIR VWFCNRRQKE
460 470 480 490 500
KRINPPSSGG SSSSPIKSLF SSPNPLVAST PSLVTSSPAT TLTVNPVLPL
510 520 530 540 550
TSAAAITSFH IPGTTGTSSA NTATVISTAP PVSSVLTSPS LSSSPSATAA
560 570 580 590 600
SSEASTAGET STTQTTSTPM TSSLNTGQVM VTASGIHTAA ATALQGAAQL
610 620 630 640 650
PTNASLAAMA AAAGLNPGLM APSQFAAGGA LFSLNPGALG SALSPALMSN
660 670 680 690 700
STLATIQALA SSGSLPITSL DAAGNLVFAN AGGTPNIVTA PLFLNPQNLS
710 720 730 740 750
LFTSNPVSLI SAASAGATGP ITSLHATTSS IDSIQNALFT MASASGAAST

TTSASKAQ
Length:758
Mass (Da):78,858
Last modified:December 15, 2009 - v3
Checksum:i807E21D83DA1985F
GO
Isoform 2 (identifier: P16143-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: E → VLE

Show »
Length:760
Mass (Da):79,070
Checksum:iB2058F94F8BD0A5D
GO

Sequence cautioni

The sequence CAA36119 differs from that shown. Unidentified sequence at N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157S → N in CAA35051 (PubMed:2326173).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038472368E → VLE in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17190 mRNA. Translation: CAA35051.1.
X57165 mRNA. Translation: CAA40454.1.
X51819 Genomic DNA. Translation: CAA36119.1. Sequence problems.
X54683 mRNA. Translation: CAA38497.1.
PIRiS07896.
RefSeqiNP_001095255.1. NM_001101785.1. [P16143-1]
UniGeneiXl.1265.

Genome annotation databases

GeneIDi399316.
KEGGixla:399316.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17190 mRNA. Translation: CAA35051.1.
X57165 mRNA. Translation: CAA40454.1.
X51819 Genomic DNA. Translation: CAA36119.1. Sequence problems.
X54683 mRNA. Translation: CAA38497.1.
PIRiS07896.
RefSeqiNP_001095255.1. NM_001101785.1. [P16143-1]
UniGeneiXl.1265.

3D structure databases

ProteinModelPortaliP16143.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP16143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399316.
KEGGixla:399316.

Organism-specific databases

CTDi5451.
XenbaseiXB-GENE-853997. pou2f1.

Phylogenomic databases

HOVERGENiHBG057016.
KOiK09364.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO2F1_XENLA
AccessioniPrimary (citable) accession number: P16143
Secondary accession number(s): Q01236, Q91809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 15, 2009
Last modified: November 2, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:2017364 and PubMed:1990276 show that although pou2f1 binds well to a perfect octamer motif, it does not bind to the variant octamer motif most often found in Xenopus laevis histone H2B gene promoters. PubMed:1406629 therefore suggest that interactions between pou2f1 and other factors bound to the H2B octamer are required for octamer-dependent H2B transcription.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.