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P16143

- PO2F1_XENLA

UniProt

P16143 - PO2F1_XENLA

Protein

POU domain, class 2, transcription factor 1

Gene

pou2f1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (15 Dec 2009)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and histone H2B. Acts downstream of Notch signaling during radial glia formation. Regulates apoptosis, possibly via an FGF-signaling pathway.6 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi395 – 45460HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: UniProtKB
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. Notch signaling pathway Source: UniProtKB
    2. positive regulation of transcription, DNA-templated Source: UniProtKB
    3. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    4. radial glial cell differentiation Source: UniProtKB
    5. regulation of apoptotic process Source: UniProtKB
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 2, transcription factor 1
    Alternative name(s):
    Octamer-binding protein 1
    Short name:
    Oct-1
    Short name:
    XOct1
    Octamer-binding transcription factor 1
    Short name:
    OTF-1
    Xloct1-32
    Gene namesi
    Name:pou2f1
    Synonyms:oct-1, oct1, oct1.32
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-853997. pou2f1.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Retained in the cytoplasm during early development, then gradually translocates to the nucleus around the mid-blastula transition (MBT).

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. transcription factor complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 758758POU domain, class 2, transcription factor 1PRO_0000100713Add
    BLAST

    Expressioni

    Tissue specificityi

    Abundant in the animal hemisphere of the blastula embryo and during early embryogenesis. Expressed in ectodermal and mesodermal cell lineages where expression becomes spatially restricted to the developing nervous system as development progresses (at protein level). Expressed in the neural plate at late neurula stage (stage 17). At early tailbud stage (stage 25), expression continues in the neural tube and begins in the eyes. At late tailbud stages (stage 30), expressed broadly in the CNS.3 Publications

    Developmental stagei

    Expressed both maternally and zygotically.3 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliP16143.
    SMRiP16143. Positions 295-455.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini294 – 36875POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi457 – 573117Ser-richAdd
    BLAST

    Domaini

    The POU domain controls nuclear translocation.1 Publication

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    HOVERGENiHBG057016.
    KOiK09364.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR000972. TF_octamer.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00029. OCTAMER.
    PR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P16143-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKLHSSSKIQ NHAWLSDARM NNPSETSKSP ESGDGNTGTQ TNGLDFQKQA    50
    VPIGAITSAQ AQALLGHLHQ VQLAGTSLQA AAHSLNVQTK FKEEPGEPMQ 100
    VVQPSQQPSL QAAIPQTQLM VAGGQIAGLT LTPAQQQMLL QQAQAQLLAA 150
    AVQHSASQQH NAAGATISAS AATPMTQIPL SQPIQIAQDL QQLQQLQQQN 200
    LNLQQYVLVH PTTNLQSAQF IISQTPQGQQ GLLQAQNLLT QLPQQSQANL 250
    LQSQPSITLT SQPATPTRTI AATPVQQLPQ SQTTPKRIDT PSLEEPSDLE 300
    ELEQFAKTFK QRRIKLGFTQ GDVGLAMGKL YGNDFSQTTI SRFEALNLSF 350
    KNMCKLKPLL EKWLNDAENI TSDSTLTNQS VLNSPGHGME GLNRRRKKRT 400
    SIETNIRVAL EKSFLENQKP TSEEITMIAD QLNMEKEVIR VWFCNRRQKE 450
    KRINPPSSGG SSSSPIKSLF SSPNPLVAST PSLVTSSPAT TLTVNPVLPL 500
    TSAAAITSFH IPGTTGTSSA NTATVISTAP PVSSVLTSPS LSSSPSATAA 550
    SSEASTAGET STTQTTSTPM TSSLNTGQVM VTASGIHTAA ATALQGAAQL 600
    PTNASLAAMA AAAGLNPGLM APSQFAAGGA LFSLNPGALG SALSPALMSN 650
    STLATIQALA SSGSLPITSL DAAGNLVFAN AGGTPNIVTA PLFLNPQNLS 700
    LFTSNPVSLI SAASAGATGP ITSLHATTSS IDSIQNALFT MASASGAAST 750
    TTSASKAQ 758
    Length:758
    Mass (Da):78,858
    Last modified:December 15, 2009 - v3
    Checksum:i807E21D83DA1985F
    GO
    Isoform 2 (identifier: P16143-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         368-368: E → VLE

    Show »
    Length:760
    Mass (Da):79,070
    Checksum:iB2058F94F8BD0A5D
    GO

    Sequence cautioni

    The sequence CAA36119.1 differs from that shown. Reason: Unidentified sequence at N-terminus.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti157 – 1571S → N in CAA35051. (PubMed:2326173)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei368 – 3681E → VLE in isoform 2. 2 PublicationsVSP_038472

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17190 mRNA. Translation: CAA35051.1.
    X57165 mRNA. Translation: CAA40454.1.
    X51819 Genomic DNA. Translation: CAA36119.1. Sequence problems.
    X54683 mRNA. Translation: CAA38497.1.
    PIRiS07896.
    RefSeqiNP_001095255.1. NM_001101785.1. [P16143-1]
    UniGeneiXl.1265.

    Genome annotation databases

    GeneIDi399316.
    KEGGixla:399316.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17190 mRNA. Translation: CAA35051.1 .
    X57165 mRNA. Translation: CAA40454.1 .
    X51819 Genomic DNA. Translation: CAA36119.1 . Sequence problems.
    X54683 mRNA. Translation: CAA38497.1 .
    PIRi S07896.
    RefSeqi NP_001095255.1. NM_001101785.1. [P16143-1 ]
    UniGenei Xl.1265.

    3D structure databases

    ProteinModelPortali P16143.
    SMRi P16143. Positions 295-455.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399316.
    KEGGi xla:399316.

    Organism-specific databases

    CTDi 5451.
    Xenbasei XB-GENE-853997. pou2f1.

    Phylogenomic databases

    HOVERGENi HBG057016.
    KOi K09364.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR000972. TF_octamer.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00029. OCTAMER.
    PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of Xenopus laevis Oct-1 cDNA."
      Smith D.P., Old R.W.
      Nucleic Acids Res. 18:369-369(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Oocyte.
    2. "Regulated binding specificity of Xenopus OCT-1, a maternal transcription factor."
      Hinkley C., Leibham D., Perry M.
      Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. Schilthuis J.G., Baarends W.M., Peterson-Maduro J., Destre O.H.J.
      Submitted (FEB-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 261-335.
    4. "Cloning and sequencing of POU-boxes expressed in Xenopus laevis neurula embryos."
      Baltzinger M., Stiegler P., Remy P.
      Nucleic Acids Res. 18:6131-6131(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-433 (ISOFORM 2).
      Tissue: Neurula.
    5. "A variant octamer motif in a Xenopus H2B histone gene promoter is not required for transcription in frog oocytes."
      Hinkley C., Perry M.
      Mol. Cell. Biol. 11:641-654(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Xenopus laevis Oct-1 does not bind to certain histone H2B gene promoter octamer motifs for which a novel octamer-binding factor has high affinity."
      Smith D.P., Old R.W.
      Nucleic Acids Res. 19:815-821(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    7. "Sequential expression of multiple POU proteins during amphibian early development."
      Hinkley C.S., Martin J.F., Leibham D., Perry M.
      Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    8. "Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs."
      Hinkley C., Perry M.
      Mol. Cell. Biol. 12:4400-4411(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA."
      Veenstra G.J., Beumer T.L., Peterson-Maduro J., Stegeman B.I., Karg H.A., van der Vliet P.C., Destree O.H.
      Mech. Dev. 50:103-117(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    10. "Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo."
      Veenstra G.J., Peterson-Maduro J., Mathu M.T., van der Vliet P.C., Destree O.H.
      Cell Death Differ. 5:774-784(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The Oct-1 POU domain directs developmentally regulated nuclear translocation in Xenopus embryos."
      Veenstra G.J., Mathu M.T., Destree O.H.
      Biol. Chem. 380:253-257(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
    12. "The POU homeobox protein Oct-1 regulates radial glia formation downstream of Notch signaling."
      Kiyota T., Kato A., Altmann C.R., Kato Y.
      Dev. Biol. 315:579-592(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPO2F1_XENLA
    AccessioniPrimary (citable) accession number: P16143
    Secondary accession number(s): Q01236, Q91809
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: December 15, 2009
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    PubMed:2017364 and PubMed:1990276 show that although pou2f1 binds well to a perfect octamer motif, it does not bind to the variant octamer motif most often found in Xenopus laevis histone H2B gene promoters. PubMed:1406629 therefore suggest that interactions between pou2f1 and other factors bound to the H2B octamer are required for octamer-dependent H2B transcription.Curated

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3