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P16143

- PO2F1_XENLA

UniProt

P16143 - PO2F1_XENLA

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Protein

POU domain, class 2, transcription factor 1

Gene

pou2f1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and histone H2B. Acts downstream of Notch signaling during radial glia formation. Regulates apoptosis, possibly via an FGF-signaling pathway.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi395 – 45460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. Notch signaling pathway Source: UniProtKB
  2. positive regulation of transcription, DNA-templated Source: UniProtKB
  3. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  4. radial glial cell differentiation Source: UniProtKB
  5. regulation of apoptotic process Source: UniProtKB
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
Octamer-binding protein 1
Short name:
Oct-1
Short name:
XOct1
Octamer-binding transcription factor 1
Short name:
OTF-1
Xloct1-32
Gene namesi
Name:pou2f1
Synonyms:oct-1, oct1, oct1.32
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-853997. pou2f1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Retained in the cytoplasm during early development, then gradually translocates to the nucleus around the mid-blastula transition (MBT).

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 758758POU domain, class 2, transcription factor 1PRO_0000100713Add
BLAST

Expressioni

Tissue specificityi

Abundant in the animal hemisphere of the blastula embryo and during early embryogenesis. Expressed in ectodermal and mesodermal cell lineages where expression becomes spatially restricted to the developing nervous system as development progresses (at protein level). Expressed in the neural plate at late neurula stage (stage 17). At early tailbud stage (stage 25), expression continues in the neural tube and begins in the eyes. At late tailbud stages (stage 30), expressed broadly in the CNS.3 Publications

Developmental stagei

Expressed both maternally and zygotically.3 Publications

Structurei

3D structure databases

ProteinModelPortaliP16143.
SMRiP16143. Positions 295-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini294 – 36875POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi457 – 573117Ser-richAdd
BLAST

Domaini

The POU domain controls nuclear translocation.1 Publication

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG057016.
KOiK09364.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P16143-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLHSSSKIQ NHAWLSDARM NNPSETSKSP ESGDGNTGTQ TNGLDFQKQA
60 70 80 90 100
VPIGAITSAQ AQALLGHLHQ VQLAGTSLQA AAHSLNVQTK FKEEPGEPMQ
110 120 130 140 150
VVQPSQQPSL QAAIPQTQLM VAGGQIAGLT LTPAQQQMLL QQAQAQLLAA
160 170 180 190 200
AVQHSASQQH NAAGATISAS AATPMTQIPL SQPIQIAQDL QQLQQLQQQN
210 220 230 240 250
LNLQQYVLVH PTTNLQSAQF IISQTPQGQQ GLLQAQNLLT QLPQQSQANL
260 270 280 290 300
LQSQPSITLT SQPATPTRTI AATPVQQLPQ SQTTPKRIDT PSLEEPSDLE
310 320 330 340 350
ELEQFAKTFK QRRIKLGFTQ GDVGLAMGKL YGNDFSQTTI SRFEALNLSF
360 370 380 390 400
KNMCKLKPLL EKWLNDAENI TSDSTLTNQS VLNSPGHGME GLNRRRKKRT
410 420 430 440 450
SIETNIRVAL EKSFLENQKP TSEEITMIAD QLNMEKEVIR VWFCNRRQKE
460 470 480 490 500
KRINPPSSGG SSSSPIKSLF SSPNPLVAST PSLVTSSPAT TLTVNPVLPL
510 520 530 540 550
TSAAAITSFH IPGTTGTSSA NTATVISTAP PVSSVLTSPS LSSSPSATAA
560 570 580 590 600
SSEASTAGET STTQTTSTPM TSSLNTGQVM VTASGIHTAA ATALQGAAQL
610 620 630 640 650
PTNASLAAMA AAAGLNPGLM APSQFAAGGA LFSLNPGALG SALSPALMSN
660 670 680 690 700
STLATIQALA SSGSLPITSL DAAGNLVFAN AGGTPNIVTA PLFLNPQNLS
710 720 730 740 750
LFTSNPVSLI SAASAGATGP ITSLHATTSS IDSIQNALFT MASASGAAST

TTSASKAQ
Length:758
Mass (Da):78,858
Last modified:December 15, 2009 - v3
Checksum:i807E21D83DA1985F
GO
Isoform 2 (identifier: P16143-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: E → VLE

Show »
Length:760
Mass (Da):79,070
Checksum:iB2058F94F8BD0A5D
GO

Sequence cautioni

The sequence CAA36119.1 differs from that shown. Reason: Unidentified sequence at N-terminus.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti157 – 1571S → N in CAA35051. (PubMed:2326173)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei368 – 3681E → VLE in isoform 2. 2 PublicationsVSP_038472

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17190 mRNA. Translation: CAA35051.1.
X57165 mRNA. Translation: CAA40454.1.
X51819 Genomic DNA. Translation: CAA36119.1. Sequence problems.
X54683 mRNA. Translation: CAA38497.1.
PIRiS07896.
RefSeqiNP_001095255.1. NM_001101785.1. [P16143-1]
UniGeneiXl.1265.

Genome annotation databases

GeneIDi399316.
KEGGixla:399316.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17190 mRNA. Translation: CAA35051.1 .
X57165 mRNA. Translation: CAA40454.1 .
X51819 Genomic DNA. Translation: CAA36119.1 . Sequence problems.
X54683 mRNA. Translation: CAA38497.1 .
PIRi S07896.
RefSeqi NP_001095255.1. NM_001101785.1. [P16143-1 ]
UniGenei Xl.1265.

3D structure databases

ProteinModelPortali P16143.
SMRi P16143. Positions 295-455.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 399316.
KEGGi xla:399316.

Organism-specific databases

CTDi 5451.
Xenbasei XB-GENE-853997. pou2f1.

Phylogenomic databases

HOVERGENi HBG057016.
KOi K09364.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of Xenopus laevis Oct-1 cDNA."
    Smith D.P., Old R.W.
    Nucleic Acids Res. 18:369-369(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Oocyte.
  2. "Regulated binding specificity of Xenopus OCT-1, a maternal transcription factor."
    Hinkley C., Leibham D., Perry M.
    Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. Schilthuis J.G., Baarends W.M., Peterson-Maduro J., Destre O.H.J.
    Submitted (FEB-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 261-335.
  4. "Cloning and sequencing of POU-boxes expressed in Xenopus laevis neurula embryos."
    Baltzinger M., Stiegler P., Remy P.
    Nucleic Acids Res. 18:6131-6131(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-433 (ISOFORM 2).
    Tissue: Neurula.
  5. "A variant octamer motif in a Xenopus H2B histone gene promoter is not required for transcription in frog oocytes."
    Hinkley C., Perry M.
    Mol. Cell. Biol. 11:641-654(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Xenopus laevis Oct-1 does not bind to certain histone H2B gene promoter octamer motifs for which a novel octamer-binding factor has high affinity."
    Smith D.P., Old R.W.
    Nucleic Acids Res. 19:815-821(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  7. "Sequential expression of multiple POU proteins during amphibian early development."
    Hinkley C.S., Martin J.F., Leibham D., Perry M.
    Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  8. "Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs."
    Hinkley C., Perry M.
    Mol. Cell. Biol. 12:4400-4411(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA."
    Veenstra G.J., Beumer T.L., Peterson-Maduro J., Stegeman B.I., Karg H.A., van der Vliet P.C., Destree O.H.
    Mech. Dev. 50:103-117(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  10. "Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo."
    Veenstra G.J., Peterson-Maduro J., Mathu M.T., van der Vliet P.C., Destree O.H.
    Cell Death Differ. 5:774-784(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The Oct-1 POU domain directs developmentally regulated nuclear translocation in Xenopus embryos."
    Veenstra G.J., Mathu M.T., Destree O.H.
    Biol. Chem. 380:253-257(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
  12. "The POU homeobox protein Oct-1 regulates radial glia formation downstream of Notch signaling."
    Kiyota T., Kato A., Altmann C.R., Kato Y.
    Dev. Biol. 315:579-592(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPO2F1_XENLA
AccessioniPrimary (citable) accession number: P16143
Secondary accession number(s): Q01236, Q91809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 15, 2009
Last modified: October 1, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:2017364 and PubMed:1990276 show that although pou2f1 binds well to a perfect octamer motif, it does not bind to the variant octamer motif most often found in Xenopus laevis histone H2B gene promoters. PubMed:1406629 therefore suggest that interactions between pou2f1 and other factors bound to the H2B octamer are required for octamer-dependent H2B transcription.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3