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P16143 (PO2F1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
Octamer-binding protein 1
Short name=Oct-1
Short name=XOct1
Octamer-binding transcription factor 1
Short name=OTF-1
Xloct1-32
Gene names
Name:pou2f1
Synonyms:oct-1, oct1, oct1.32
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and histone H2B. Acts downstream of Notch signaling during radial glia formation. Regulates apoptosis, possibly via an FGF-signaling pathway. Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.12

Subcellular location

Cytoplasm. Nucleus. Note: Retained in the cytoplasm during early development, then gradually translocates to the nucleus around the mid-blastula transition (MBT). Ref.6 Ref.11 Ref.12

Tissue specificity

Abundant in the animal hemisphere of the blastula embryo and during early embryogenesis. Expressed in ectodermal and mesodermal cell lineages where expression becomes spatially restricted to the developing nervous system as development progresses (at protein level). Expressed in the neural plate at late neurula stage (stage 17). At early tailbud stage (stage 25), expression continues in the neural tube and begins in the eyes. At late tailbud stages (stage 30), expressed broadly in the CNS. Ref.9 Ref.11 Ref.12

Developmental stage

Expressed both maternally and zygotically. Ref.6 Ref.7 Ref.9

Domain

The POU domain controls nuclear translocation. Ref.11

Sequence similarities

Belongs to the POU transcription factor family. Class-2 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Caution

Ref.6 and Ref.5 show that although pou2f1 binds well to a perfect octamer motif, it does not bind to the variant octamer motif most often found in Xenopus laevis histone H2B gene promoters. Ref.8 therefore suggest that interactions between pou2f1 and other factors bound to the H2B octamer are required for octamer-dependent H2B transcription.

Sequence caution

The sequence CAA36119.1 differs from that shown. Reason: Unidentified sequence at N-terminus.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P16143-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P16143-2)

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: E → VLE

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758POU domain, class 2, transcription factor 1
PRO_0000100713

Regions

Domain294 – 36875POU-specific
DNA binding395 – 45460Homeobox
Compositional bias457 – 573117Ser-rich

Natural variations

Alternative sequence3681E → VLE in isoform 2.
VSP_038472

Experimental info

Sequence conflict1571S → N in CAA35051. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2009. Version 3.
Checksum: 807E21D83DA1985F

FASTA75878,858
        10         20         30         40         50         60 
MKLHSSSKIQ NHAWLSDARM NNPSETSKSP ESGDGNTGTQ TNGLDFQKQA VPIGAITSAQ 

        70         80         90        100        110        120 
AQALLGHLHQ VQLAGTSLQA AAHSLNVQTK FKEEPGEPMQ VVQPSQQPSL QAAIPQTQLM 

       130        140        150        160        170        180 
VAGGQIAGLT LTPAQQQMLL QQAQAQLLAA AVQHSASQQH NAAGATISAS AATPMTQIPL 

       190        200        210        220        230        240 
SQPIQIAQDL QQLQQLQQQN LNLQQYVLVH PTTNLQSAQF IISQTPQGQQ GLLQAQNLLT 

       250        260        270        280        290        300 
QLPQQSQANL LQSQPSITLT SQPATPTRTI AATPVQQLPQ SQTTPKRIDT PSLEEPSDLE 

       310        320        330        340        350        360 
ELEQFAKTFK QRRIKLGFTQ GDVGLAMGKL YGNDFSQTTI SRFEALNLSF KNMCKLKPLL 

       370        380        390        400        410        420 
EKWLNDAENI TSDSTLTNQS VLNSPGHGME GLNRRRKKRT SIETNIRVAL EKSFLENQKP 

       430        440        450        460        470        480 
TSEEITMIAD QLNMEKEVIR VWFCNRRQKE KRINPPSSGG SSSSPIKSLF SSPNPLVAST 

       490        500        510        520        530        540 
PSLVTSSPAT TLTVNPVLPL TSAAAITSFH IPGTTGTSSA NTATVISTAP PVSSVLTSPS 

       550        560        570        580        590        600 
LSSSPSATAA SSEASTAGET STTQTTSTPM TSSLNTGQVM VTASGIHTAA ATALQGAAQL 

       610        620        630        640        650        660 
PTNASLAAMA AAAGLNPGLM APSQFAAGGA LFSLNPGALG SALSPALMSN STLATIQALA 

       670        680        690        700        710        720 
SSGSLPITSL DAAGNLVFAN AGGTPNIVTA PLFLNPQNLS LFTSNPVSLI SAASAGATGP 

       730        740        750 
ITSLHATTSS IDSIQNALFT MASASGAAST TTSASKAQ 

« Hide

Isoform 2 [UniParc].

Checksum: B2058F94F8BD0A5D
Show »

FASTA76079,070

References

[1]"Nucleotide sequence of Xenopus laevis Oct-1 cDNA."
Smith D.P., Old R.W.
Nucleic Acids Res. 18:369-369(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Oocyte.
[2]"Regulated binding specificity of Xenopus OCT-1, a maternal transcription factor."
Hinkley C., Leibham D., Perry M.
Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]Schilthuis J.G., Baarends W.M., Peterson-Maduro J., Destre O.H.J.
Submitted (FEB-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 261-335.
[4]"Cloning and sequencing of POU-boxes expressed in Xenopus laevis neurula embryos."
Baltzinger M., Stiegler P., Remy P.
Nucleic Acids Res. 18:6131-6131(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-433 (ISOFORM 2).
Tissue: Neurula.
[5]"A variant octamer motif in a Xenopus H2B histone gene promoter is not required for transcription in frog oocytes."
Hinkley C., Perry M.
Mol. Cell. Biol. 11:641-654(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Xenopus laevis Oct-1 does not bind to certain histone H2B gene promoter octamer motifs for which a novel octamer-binding factor has high affinity."
Smith D.P., Old R.W.
Nucleic Acids Res. 19:815-821(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[7]"Sequential expression of multiple POU proteins during amphibian early development."
Hinkley C.S., Martin J.F., Leibham D., Perry M.
Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[8]"Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs."
Hinkley C., Perry M.
Mol. Cell. Biol. 12:4400-4411(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA."
Veenstra G.J., Beumer T.L., Peterson-Maduro J., Stegeman B.I., Karg H.A., van der Vliet P.C., Destree O.H.
Mech. Dev. 50:103-117(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[10]"Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo."
Veenstra G.J., Peterson-Maduro J., Mathu M.T., van der Vliet P.C., Destree O.H.
Cell Death Differ. 5:774-784(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"The Oct-1 POU domain directs developmentally regulated nuclear translocation in Xenopus embryos."
Veenstra G.J., Mathu M.T., Destree O.H.
Biol. Chem. 380:253-257(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
[12]"The POU homeobox protein Oct-1 regulates radial glia formation downstream of Notch signaling."
Kiyota T., Kato A., Altmann C.R., Kato Y.
Dev. Biol. 315:579-592(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17190 mRNA. Translation: CAA35051.1.
X57165 mRNA. Translation: CAA40454.1.
X51819 Genomic DNA. Translation: CAA36119.1. Sequence problems.
X54683 mRNA. Translation: CAA38497.1.
PIRS07896.
RefSeqNP_001095255.1. NM_001101785.1.
UniGeneXl.1265.

3D structure databases

ProteinModelPortalP16143.
SMRP16143. Positions 295-455.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399316.
KEGGxla:399316.

Organism-specific databases

CTD5451.
XenbaseXB-GENE-853997. pou2f1.

Phylogenomic databases

HOVERGENHBG057016.
KOK09364.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePO2F1_XENLA
AccessionPrimary (citable) accession number: P16143
Secondary accession number(s): Q01236, Q91809
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 15, 2009
Last modified: April 16, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families