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P16142

- MDH_METFV

UniProt

P16142 - MDH_METFV

Protein

Malate/(S)-sulfolactate dehydrogenase

Gene

mdh

Organism
Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH.1 Publication

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.
    (S)-malate + NADP+ = oxaloacetate + NADPH.
    (2S)-3-sulfolactate + NAD+ = 3-sulfopyruvate + NADH.

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC
    2. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    BioCyciMFER523846:GC24-404-MONOMER.
    SABIO-RKP16142.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate/(S)-sulfolactate dehydrogenase (EC:1.1.1.310, EC:1.1.1.37, EC:1.1.1.82)
    Gene namesi
    Name:mdh
    Ordered Locus Names:Mfer_0389
    OrganismiMethanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
    Taxonomic identifieri523846 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus
    ProteomesiUP000002315: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Malate/(S)-sulfolactate dehydrogenasePRO_0000083827Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP16142.
    SMRiP16142. Positions 1-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH2/MDH2 oxidoreductase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000173270.
    KOiK05884.
    OMAiENGNINP.

    Family and domain databases

    InterProiIPR003767. Malate/L-lactate_DH.
    [Graphical view]
    PANTHERiPTHR11091. PTHR11091. 1 hit.
    PfamiPF02615. Ldh_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF89733. SSF89733. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P16142-1 [UniParc]FASTAAdd to Basket

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    MIISPEEERS LIIKILNALG VSEEHAKITA DVIVDADLKG FTSHGIGRFP    50
    QYVEGIKLGT IKTSGNIEIE KETDSVALIN GNHLLGQVVA YKGMKLAIEK 100
    AKNTGVGIVG IHDSNHFGIA GYYSDMAMKN DMIGITMTNT EPAVAPLGGK 150
    IPVLGTNPIA ISIPSNEYYV AVDMSTAAVA RGKLLEAARK NEKIPEGIAV 200
    DKNGNPTTDP NEALNGSILP FGGHKGYALC FMIEILAGPL VKAEFGSKVK 250
    GTVDPSQMCT KGDLLIAIDP SKFYDIEEFK RNVDEFVKEI KSTGKDVLIP 300
    GDRERMNIKK REKEGIELDK KLVEKLKEIA DELNIELTW 339
    Length:339
    Mass (Da):36,762
    Last modified:August 1, 1990 - v2
    Checksum:i2319D822DB275835
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51714 Genomic DNA. Translation: CAA36010.1.
    X51840 Genomic DNA. Translation: CAA36133.1.
    CP002278 Genomic DNA. Translation: ADP77191.1.
    PIRiS08981.
    RefSeqiWP_013413469.1. NC_014658.1.
    YP_004003953.1. NC_014658.1.

    Genome annotation databases

    EnsemblBacteriaiADP77191; ADP77191; Mfer_0389.
    GeneIDi9962106.
    KEGGimfv:Mfer_0389.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51714 Genomic DNA. Translation: CAA36010.1 .
    X51840 Genomic DNA. Translation: CAA36133.1 .
    CP002278 Genomic DNA. Translation: ADP77191.1 .
    PIRi S08981.
    RefSeqi WP_013413469.1. NC_014658.1.
    YP_004003953.1. NC_014658.1.

    3D structure databases

    ProteinModelPortali P16142.
    SMRi P16142. Positions 1-338.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADP77191 ; ADP77191 ; Mfer_0389 .
    GeneIDi 9962106.
    KEGGi mfv:Mfer_0389.

    Phylogenomic databases

    HOGENOMi HOG000173270.
    KOi K05884.
    OMAi ENGNINP.

    Enzyme and pathway databases

    BioCyci MFER523846:GC24-404-MONOMER.
    SABIO-RK P16142.

    Family and domain databases

    InterProi IPR003767. Malate/L-lactate_DH.
    [Graphical view ]
    PANTHERi PTHR11091. PTHR11091. 1 hit.
    Pfami PF02615. Ldh_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF89733. SSF89733. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Properties and primary structure of the L-malate dehydrogenase from the extremely thermophilic archaebacterium Methanothermus fervidus."
      Honka E., Fabry S., Niermann T., Palm P., Hensel R.
      Eur. J. Biochem. 188:623-632(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24.
      Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
    3. "Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea."
      Graupner M., Xu H., White R.H.
      J. Bacteriol. 182:3688-3692(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMDH_METFV
    AccessioniPrimary (citable) accession number: P16142
    Secondary accession number(s): E3GY09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3