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Reviewed, UniProtKB/Swiss-Prot P16142 (MDH_METFE)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Malate/L-sulfolactate dehydrogenase
    EC=1.1.1.37
    EC=1.1.1.82
    EC=1.1.1.272
Alternative name(s):
    (R)-2-hydroxyacid dehydrogenase
Gene names
Name: mdh
OrganismMethanothermus fervidus
Taxonomic identifier2180 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH. Ref.2

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

(S)-malate + NADP+ = oxaloacetate + NADPH.

(2R)-3-sulfolactate + NAD(P)+ = 3-sulfopyruvate + NAD(P)H.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the LDH2/MDH2 oxidoreductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Malate/L-sulfolactate dehydrogenase
PRO_0000083827

Sequences

Sequence LengthMass (Da)Tools
P16142-1 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: 2319D822DB275835

FASTA33936,762
        10         20         30         40         50         60 
MIISPEEERS LIIKILNALG VSEEHAKITA DVIVDADLKG FTSHGIGRFP QYVEGIKLGT 

        70         80         90        100        110        120 
IKTSGNIEIE KETDSVALIN GNHLLGQVVA YKGMKLAIEK AKNTGVGIVG IHDSNHFGIA 

       130        140        150        160        170        180 
GYYSDMAMKN DMIGITMTNT EPAVAPLGGK IPVLGTNPIA ISIPSNEYYV AVDMSTAAVA 

       190        200        210        220        230        240 
RGKLLEAARK NEKIPEGIAV DKNGNPTTDP NEALNGSILP FGGHKGYALC FMIEILAGPL 

       250        260        270        280        290        300 
VKAEFGSKVK GTVDPSQMCT KGDLLIAIDP SKFYDIEEFK RNVDEFVKEI KSTGKDVLIP 

       310        320        330 
GDRERMNIKK REKEGIELDK KLVEKLKEIA DELNIELTW 

« Hide

References

[1]"Properties and primary structure of the L-malate dehydrogenase from the extremely thermophilic archaebacterium Methanothermus fervidus."
Honka E., Fabry S., Niermann T., Palm P., Hensel R.
Eur. J. Biochem. 188:623-632(1990) [PubMed: 2110059] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24.
Strain: DSM 2088 / V24S.
[2]"Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea."
Graupner M., Xu H., White R.H.
J. Bacteriol. 182:3688-3692(2000) [PubMed: 10850983] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

X51714 Genomic DNA. Translation: CAA36010.1.
X51840 Genomic DNA. Translation: CAA36133.1.
PIRS08981.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.272. 7358.
1.1.1.37. 7358.
1.1.1.82. 7358.

Family and domain databases

InterProIPR003767. Malate/L-lactate_DH.
[Graphical view]
PANTHERPTHR11091. MDH_LDH. 1 hit.
PfamPF02615. Ldh_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_METFE
AccessionPrimary (citable) accession number: P16142
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1990
Last modified: June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents