Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P16142

- MDH_METFV

UniProt

P16142 - MDH_METFV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate/(S)-sulfolactate dehydrogenase

Gene

mdh

Organism
Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.
(S)-malate + NADP+ = oxaloacetate + NADPH.
(2S)-3-sulfolactate + NAD+ = 3-sulfopyruvate + NADH.

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC
  2. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciMFER523846:GC24-404-MONOMER.
SABIO-RKP16142.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate/(S)-sulfolactate dehydrogenase (EC:1.1.1.310, EC:1.1.1.37, EC:1.1.1.82)
Gene namesi
Name:mdh
Ordered Locus Names:Mfer_0389
OrganismiMethanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
Taxonomic identifieri523846 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus
ProteomesiUP000002315: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Malate/(S)-sulfolactate dehydrogenasePRO_0000083827Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP16142.
SMRiP16142. Positions 1-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH2/MDH2 oxidoreductase family.Curated

Phylogenomic databases

HOGENOMiHOG000173270.
KOiK05884.
OMAiENGNINP.

Family and domain databases

InterProiIPR003767. Malate/L-lactate_DH.
[Graphical view]
PANTHERiPTHR11091. PTHR11091. 1 hit.
PfamiPF02615. Ldh_2. 1 hit.
[Graphical view]
SUPFAMiSSF89733. SSF89733. 1 hit.

Sequencei

Sequence statusi: Complete.

P16142-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIISPEEERS LIIKILNALG VSEEHAKITA DVIVDADLKG FTSHGIGRFP
60 70 80 90 100
QYVEGIKLGT IKTSGNIEIE KETDSVALIN GNHLLGQVVA YKGMKLAIEK
110 120 130 140 150
AKNTGVGIVG IHDSNHFGIA GYYSDMAMKN DMIGITMTNT EPAVAPLGGK
160 170 180 190 200
IPVLGTNPIA ISIPSNEYYV AVDMSTAAVA RGKLLEAARK NEKIPEGIAV
210 220 230 240 250
DKNGNPTTDP NEALNGSILP FGGHKGYALC FMIEILAGPL VKAEFGSKVK
260 270 280 290 300
GTVDPSQMCT KGDLLIAIDP SKFYDIEEFK RNVDEFVKEI KSTGKDVLIP
310 320 330
GDRERMNIKK REKEGIELDK KLVEKLKEIA DELNIELTW
Length:339
Mass (Da):36,762
Last modified:August 1, 1990 - v2
Checksum:i2319D822DB275835
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51714 Genomic DNA. Translation: CAA36010.1.
X51840 Genomic DNA. Translation: CAA36133.1.
CP002278 Genomic DNA. Translation: ADP77191.1.
PIRiS08981.
RefSeqiWP_013413469.1. NC_014658.1.
YP_004003953.1. NC_014658.1.

Genome annotation databases

EnsemblBacteriaiADP77191; ADP77191; Mfer_0389.
GeneIDi9962106.
KEGGimfv:Mfer_0389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51714 Genomic DNA. Translation: CAA36010.1 .
X51840 Genomic DNA. Translation: CAA36133.1 .
CP002278 Genomic DNA. Translation: ADP77191.1 .
PIRi S08981.
RefSeqi WP_013413469.1. NC_014658.1.
YP_004003953.1. NC_014658.1.

3D structure databases

ProteinModelPortali P16142.
SMRi P16142. Positions 1-338.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADP77191 ; ADP77191 ; Mfer_0389 .
GeneIDi 9962106.
KEGGi mfv:Mfer_0389.

Phylogenomic databases

HOGENOMi HOG000173270.
KOi K05884.
OMAi ENGNINP.

Enzyme and pathway databases

BioCyci MFER523846:GC24-404-MONOMER.
SABIO-RK P16142.

Family and domain databases

InterProi IPR003767. Malate/L-lactate_DH.
[Graphical view ]
PANTHERi PTHR11091. PTHR11091. 1 hit.
Pfami PF02615. Ldh_2. 1 hit.
[Graphical view ]
SUPFAMi SSF89733. SSF89733. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Properties and primary structure of the L-malate dehydrogenase from the extremely thermophilic archaebacterium Methanothermus fervidus."
    Honka E., Fabry S., Niermann T., Palm P., Hensel R.
    Eur. J. Biochem. 188:623-632(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24.
    Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
  3. "Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea."
    Graupner M., Xu H., White R.H.
    J. Bacteriol. 182:3688-3692(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMDH_METFV
AccessioniPrimary (citable) accession number: P16142
Secondary accession number(s): E3GY09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3