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P16120

- THRC_YEAST

UniProt

P16120 - THRC_YEAST

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Protein

Threonine synthase

Gene

THR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity

Catalytic activityi

O-phospho-L-homoserine + H2O = L-threonine + phosphate.

Cofactori

Pyridoxal phosphate.By similarity

Pathwayi

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. threonine synthase activity Source: SGD

GO - Biological processi

  1. threonine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YCR053W-MONOMER.
UniPathwayiUPA00050; UER00065.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthase (EC:4.2.3.1)
Short name:
TS
Gene namesi
Name:THR4
Ordered Locus Names:YCR053W
ORF Names:YCR53W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR053w.
SGDiS000000649. THR4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Threonine synthasePRO_0000185645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei124 – 1241N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei467 – 4671Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP16120.
PaxDbiP16120.
PeptideAtlasiP16120.

Expressioni

Gene expression databases

GenevestigatoriP16120.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSM4P403181EBI-19259,EBI-18208

Protein-protein interaction databases

BioGridi31033. 337 interactions.
DIPiDIP-6522N.
IntActiP16120. 1 interaction.
MINTiMINT-2787019.
STRINGi4932.YCR053W.

Structurei

Secondary structure

1
514
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103
Helixi21 – 277
Helixi46 – 516
Turni52 – 554
Helixi58 – 6710
Turni72 – 743
Helixi77 – 8711
Turni88 – 903
Beta strandi106 – 1083
Beta strandi110 – 1145
Helixi125 – 14319
Beta strandi148 – 1503
Beta strandi154 – 1596
Beta strandi161 – 1633
Helixi164 – 1729
Beta strandi178 – 1858
Helixi191 – 1999
Beta strandi205 – 2128
Helixi214 – 22613
Helixi244 – 26219
Beta strandi263 – 2664
Beta strandi270 – 2756
Beta strandi277 – 2793
Helixi280 – 29112
Beta strandi298 – 3025
Helixi307 – 3148
Beta strandi315 – 3184
Helixi329 – 3313
Helixi339 – 35012
Helixi356 – 37318
Beta strandi374 – 3774
Helixi380 – 3867
Turni387 – 3893
Beta strandi390 – 3945
Helixi397 – 41014
Helixi421 – 43717
Beta strandi442 – 4487
Helixi452 – 4554
Helixi456 – 4638
Helixi471 – 4744
Helixi478 – 4814
Helixi483 – 4853
Helixi498 – 5069

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KL7X-ray2.70A/B1-514[»]
ProteinModelPortaliP16120.
SMRiP16120. Positions 2-512.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16120.

Family & Domainsi

Sequence similaritiesi

Belongs to the threonine synthase family.Curated

Phylogenomic databases

eggNOGiCOG0498.
GeneTreeiENSGT00530000063879.
HOGENOMiHOG000230745.
InParanoidiP16120.
KOiK01733.
OMAiILLWLES.
OrthoDBiEOG7N0CDH.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16120-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPNASQVYRS TRSSSPKTIS FEEAIIQGLA TDGGLFIPPT IPQVDQATLF
60 70 80 90 100
NDWSKLSFQD LAFAIMRLYI AQEEIPDADL KDLIKRSYST FRSDEVTPLV
110 120 130 140 150
QNVTGDKENL HILELFHGPT YAFKDVALQF VGNLFEYFLQ RTNANLPEGE
160 170 180 190 200
KKQITVVGAT SGDTGSAAIY GLRGKKDVSV FILYPTGRIS PIQEEQMTTV
210 220 230 240 250
PDENVQTLSV TGTFDNCQDI VKAIFGDKEF NSKHNVGAVN SINWARILAQ
260 270 280 290 300
MTYYFYSFFQ ATNGKDSKKV KFVVPSGNFG DILAGYFAKK MGLPIEKLAI
310 320 330 340 350
ATNENDILDR FLKSGLYERS DKVAATLSPA MDILISSNFE RLLWYLAREY
360 370 380 390 400
LANGDDLKAG EIVNNWFQEL KTNGKFQVDK SIIEGASKDF TSERVSNEET
410 420 430 440 450
SETIKKIYES SVNPKHYILD PHTAVGVCAT ERLIAKDNDK SIQYISLSTA
460 470 480 490 500
HPAKFADAVN NALSGFSNYS FEKDVLPEEL KKLSTLKKKL KFIERADVEL
510
VKNAIEEELA KMKL
Length:514
Mass (Da):57,474
Last modified:April 1, 1990 - v1
Checksum:iEF6BA9DF56D94B6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761K → R in AAU09679. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17256 Genomic DNA. Translation: CAA35157.1.
X59720 Genomic DNA. Translation: CAA42284.1.
AY723762 Genomic DNA. Translation: AAU09679.1.
BK006937 Genomic DNA. Translation: DAA07530.1.
PIRiS22836. SYBSR.
RefSeqiNP_009982.1. NM_001178767.1.

Genome annotation databases

EnsemblFungiiYCR053W; YCR053W; YCR053W.
GeneIDi850420.
KEGGisce:YCR053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17256 Genomic DNA. Translation: CAA35157.1 .
X59720 Genomic DNA. Translation: CAA42284.1 .
AY723762 Genomic DNA. Translation: AAU09679.1 .
BK006937 Genomic DNA. Translation: DAA07530.1 .
PIRi S22836. SYBSR.
RefSeqi NP_009982.1. NM_001178767.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KL7 X-ray 2.70 A/B 1-514 [» ]
ProteinModelPortali P16120.
SMRi P16120. Positions 2-512.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31033. 337 interactions.
DIPi DIP-6522N.
IntActi P16120. 1 interaction.
MINTi MINT-2787019.
STRINGi 4932.YCR053W.

Proteomic databases

MaxQBi P16120.
PaxDbi P16120.
PeptideAtlasi P16120.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR053W ; YCR053W ; YCR053W .
GeneIDi 850420.
KEGGi sce:YCR053W.

Organism-specific databases

CYGDi YCR053w.
SGDi S000000649. THR4.

Phylogenomic databases

eggNOGi COG0498.
GeneTreei ENSGT00530000063879.
HOGENOMi HOG000230745.
InParanoidi P16120.
KOi K01733.
OMAi ILLWLES.
OrthoDBi EOG7N0CDH.

Enzyme and pathway databases

UniPathwayi UPA00050 ; UER00065 .
BioCyci YEAST:YCR053W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P16120.
NextBioi 965986.
PROi P16120.

Gene expression databases

Genevestigatori P16120.

Family and domain databases

Gene3Di 3.90.1380.10. 1 hit.
InterProi IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view ]
Pfami PF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53686. SSF53686. 1 hit.
TIGRFAMsi TIGR00260. thrC. 1 hit.
PROSITEi PS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the yeast THR4 gene encoding threonine synthase."
    Aas S.F., Rognes S.E.
    Nucleic Acids Res. 18:665-665(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26786 / X2180-1A.
  2. "Analysis of the THR4 region on chromosome III of the yeast Saccharomyces cerevisiae."
    Mannhaupt G., van der Linden C.G., Vetter I., Maurer K., Pilz U., Planta R.J., Feldmann H.
    Yeast 6:353-361(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTHRC_YEAST
AccessioniPrimary (citable) accession number: P16120
Secondary accession number(s): D6VR61, E9P943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 26000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3