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P16120

- THRC_YEAST

UniProt

P16120 - THRC_YEAST

Protein

Threonine synthase

Gene

THR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity

    Catalytic activityi

    O-phospho-L-homoserine + H2O = L-threonine + phosphate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. threonine synthase activity Source: SGD

    GO - Biological processi

    1. threonine biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Threonine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YCR053W-MONOMER.
    UniPathwayiUPA00050; UER00065.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine synthase (EC:4.2.3.1)
    Short name:
    TS
    Gene namesi
    Name:THR4
    Ordered Locus Names:YCR053W
    ORF Names:YCR53W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR053w.
    SGDiS000000649. THR4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 514514Threonine synthasePRO_0000185645Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei124 – 1241N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei467 – 4671Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP16120.
    PaxDbiP16120.
    PeptideAtlasiP16120.

    Expressioni

    Gene expression databases

    GenevestigatoriP16120.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SSM4P403181EBI-19259,EBI-18208

    Protein-protein interaction databases

    BioGridi31033. 336 interactions.
    DIPiDIP-6522N.
    IntActiP16120. 1 interaction.
    MINTiMINT-2787019.
    STRINGi4932.YCR053W.

    Structurei

    Secondary structure

    1
    514
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 103
    Helixi21 – 277
    Helixi46 – 516
    Turni52 – 554
    Helixi58 – 6710
    Turni72 – 743
    Helixi77 – 8711
    Turni88 – 903
    Beta strandi106 – 1083
    Beta strandi110 – 1145
    Helixi125 – 14319
    Beta strandi148 – 1503
    Beta strandi154 – 1596
    Beta strandi161 – 1633
    Helixi164 – 1729
    Beta strandi178 – 1858
    Helixi191 – 1999
    Beta strandi205 – 2128
    Helixi214 – 22613
    Helixi244 – 26219
    Beta strandi263 – 2664
    Beta strandi270 – 2756
    Beta strandi277 – 2793
    Helixi280 – 29112
    Beta strandi298 – 3025
    Helixi307 – 3148
    Beta strandi315 – 3184
    Helixi329 – 3313
    Helixi339 – 35012
    Helixi356 – 37318
    Beta strandi374 – 3774
    Helixi380 – 3867
    Turni387 – 3893
    Beta strandi390 – 3945
    Helixi397 – 41014
    Helixi421 – 43717
    Beta strandi442 – 4487
    Helixi452 – 4554
    Helixi456 – 4638
    Helixi471 – 4744
    Helixi478 – 4814
    Helixi483 – 4853
    Helixi498 – 5069

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KL7X-ray2.70A/B1-514[»]
    ProteinModelPortaliP16120.
    SMRiP16120. Positions 2-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16120.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the threonine synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0498.
    GeneTreeiENSGT00530000063879.
    HOGENOMiHOG000230745.
    KOiK01733.
    OMAiILLWLES.
    OrthoDBiEOG7N0CDH.

    Family and domain databases

    Gene3Di3.90.1380.10. 1 hit.
    InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR029144. Thr_synth_N.
    IPR004450. Thr_synthase_like.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    PF14821. Thr_synth_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR00260. thrC. 1 hit.
    PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16120-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNASQVYRS TRSSSPKTIS FEEAIIQGLA TDGGLFIPPT IPQVDQATLF    50
    NDWSKLSFQD LAFAIMRLYI AQEEIPDADL KDLIKRSYST FRSDEVTPLV 100
    QNVTGDKENL HILELFHGPT YAFKDVALQF VGNLFEYFLQ RTNANLPEGE 150
    KKQITVVGAT SGDTGSAAIY GLRGKKDVSV FILYPTGRIS PIQEEQMTTV 200
    PDENVQTLSV TGTFDNCQDI VKAIFGDKEF NSKHNVGAVN SINWARILAQ 250
    MTYYFYSFFQ ATNGKDSKKV KFVVPSGNFG DILAGYFAKK MGLPIEKLAI 300
    ATNENDILDR FLKSGLYERS DKVAATLSPA MDILISSNFE RLLWYLAREY 350
    LANGDDLKAG EIVNNWFQEL KTNGKFQVDK SIIEGASKDF TSERVSNEET 400
    SETIKKIYES SVNPKHYILD PHTAVGVCAT ERLIAKDNDK SIQYISLSTA 450
    HPAKFADAVN NALSGFSNYS FEKDVLPEEL KKLSTLKKKL KFIERADVEL 500
    VKNAIEEELA KMKL 514
    Length:514
    Mass (Da):57,474
    Last modified:April 1, 1990 - v1
    Checksum:iEF6BA9DF56D94B6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti176 – 1761K → R in AAU09679. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17256 Genomic DNA. Translation: CAA35157.1.
    X59720 Genomic DNA. Translation: CAA42284.1.
    AY723762 Genomic DNA. Translation: AAU09679.1.
    BK006937 Genomic DNA. Translation: DAA07530.1.
    PIRiS22836. SYBSR.
    RefSeqiNP_009982.1. NM_001178767.1.

    Genome annotation databases

    EnsemblFungiiYCR053W; YCR053W; YCR053W.
    GeneIDi850420.
    KEGGisce:YCR053W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17256 Genomic DNA. Translation: CAA35157.1 .
    X59720 Genomic DNA. Translation: CAA42284.1 .
    AY723762 Genomic DNA. Translation: AAU09679.1 .
    BK006937 Genomic DNA. Translation: DAA07530.1 .
    PIRi S22836. SYBSR.
    RefSeqi NP_009982.1. NM_001178767.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KL7 X-ray 2.70 A/B 1-514 [» ]
    ProteinModelPortali P16120.
    SMRi P16120. Positions 2-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31033. 336 interactions.
    DIPi DIP-6522N.
    IntActi P16120. 1 interaction.
    MINTi MINT-2787019.
    STRINGi 4932.YCR053W.

    Proteomic databases

    MaxQBi P16120.
    PaxDbi P16120.
    PeptideAtlasi P16120.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR053W ; YCR053W ; YCR053W .
    GeneIDi 850420.
    KEGGi sce:YCR053W.

    Organism-specific databases

    CYGDi YCR053w.
    SGDi S000000649. THR4.

    Phylogenomic databases

    eggNOGi COG0498.
    GeneTreei ENSGT00530000063879.
    HOGENOMi HOG000230745.
    KOi K01733.
    OMAi ILLWLES.
    OrthoDBi EOG7N0CDH.

    Enzyme and pathway databases

    UniPathwayi UPA00050 ; UER00065 .
    BioCyci YEAST:YCR053W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P16120.
    NextBioi 965986.
    PROi P16120.

    Gene expression databases

    Genevestigatori P16120.

    Family and domain databases

    Gene3Di 3.90.1380.10. 1 hit.
    InterProi IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR029144. Thr_synth_N.
    IPR004450. Thr_synthase_like.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view ]
    Pfami PF00291. PALP. 1 hit.
    PF14821. Thr_synth_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR00260. thrC. 1 hit.
    PROSITEi PS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the yeast THR4 gene encoding threonine synthase."
      Aas S.F., Rognes S.E.
      Nucleic Acids Res. 18:665-665(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26786 / X2180-1A.
    2. "Analysis of the THR4 region on chromosome III of the yeast Saccharomyces cerevisiae."
      Mannhaupt G., van der Linden C.G., Vetter I., Maurer K., Pilz U., Planta R.J., Feldmann H.
      Yeast 6:353-361(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTHRC_YEAST
    AccessioniPrimary (citable) accession number: P16120
    Secondary accession number(s): D6VR61, E9P943
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 26000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3