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Protein

Threonine synthase

Gene

THR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity

Catalytic activityi

O-phospho-L-homoserine + H2O = L-threonine + phosphate.

Cofactori

Pathwayi: L-threonine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
  4. Homoserine kinase (THR1)
  5. Threonine synthase (THR4)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • pyridoxal phosphate binding Source: GO_Central
  • threonine synthase activity Source: SGD

GO - Biological processi

  • dephosphorylation Source: GO_Central
  • threonine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YCR053W-MONOMER.
UniPathwayiUPA00050; UER00065.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthase (EC:4.2.3.1)
Short name:
TS
Gene namesi
Name:THR4
Ordered Locus Names:YCR053W
ORF Names:YCR53W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR053W.
SGDiS000000649. THR4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001856451 – 514Threonine synthaseAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei467PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP16120.
PRIDEiP16120.
TopDownProteomicsiP16120.

PTM databases

iPTMnetiP16120.

Interactioni

Protein-protein interaction databases

BioGridi31033. 337 interactors.
DIPiDIP-6522N.
IntActiP16120. 1 interactor.
MINTiMINT-2787019.

Structurei

Secondary structure

1514
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Helixi21 – 27Combined sources7
Helixi46 – 51Combined sources6
Turni52 – 55Combined sources4
Helixi58 – 67Combined sources10
Turni72 – 74Combined sources3
Helixi77 – 87Combined sources11
Turni88 – 90Combined sources3
Beta strandi106 – 108Combined sources3
Beta strandi110 – 114Combined sources5
Helixi125 – 143Combined sources19
Beta strandi148 – 150Combined sources3
Beta strandi154 – 159Combined sources6
Beta strandi161 – 163Combined sources3
Helixi164 – 172Combined sources9
Beta strandi178 – 185Combined sources8
Helixi191 – 199Combined sources9
Beta strandi205 – 212Combined sources8
Helixi214 – 226Combined sources13
Helixi244 – 262Combined sources19
Beta strandi263 – 266Combined sources4
Beta strandi270 – 275Combined sources6
Beta strandi277 – 279Combined sources3
Helixi280 – 291Combined sources12
Beta strandi298 – 302Combined sources5
Helixi307 – 314Combined sources8
Beta strandi315 – 318Combined sources4
Helixi329 – 331Combined sources3
Helixi339 – 350Combined sources12
Helixi356 – 373Combined sources18
Beta strandi374 – 377Combined sources4
Helixi380 – 386Combined sources7
Turni387 – 389Combined sources3
Beta strandi390 – 394Combined sources5
Helixi397 – 410Combined sources14
Helixi421 – 437Combined sources17
Beta strandi442 – 448Combined sources7
Helixi452 – 455Combined sources4
Helixi456 – 463Combined sources8
Helixi471 – 474Combined sources4
Helixi478 – 481Combined sources4
Helixi483 – 485Combined sources3
Helixi498 – 506Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KL7X-ray2.70A/B1-514[»]
ProteinModelPortaliP16120.
SMRiP16120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16120.

Family & Domainsi

Sequence similaritiesi

Belongs to the threonine synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063879.
HOGENOMiHOG000230745.
InParanoidiP16120.
KOiK01733.
OMAiEEIASWA.
OrthoDBiEOG092C1BXU.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR029144. Thr_synth_N.
IPR004450. Thr_synthase-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNASQVYRS TRSSSPKTIS FEEAIIQGLA TDGGLFIPPT IPQVDQATLF
60 70 80 90 100
NDWSKLSFQD LAFAIMRLYI AQEEIPDADL KDLIKRSYST FRSDEVTPLV
110 120 130 140 150
QNVTGDKENL HILELFHGPT YAFKDVALQF VGNLFEYFLQ RTNANLPEGE
160 170 180 190 200
KKQITVVGAT SGDTGSAAIY GLRGKKDVSV FILYPTGRIS PIQEEQMTTV
210 220 230 240 250
PDENVQTLSV TGTFDNCQDI VKAIFGDKEF NSKHNVGAVN SINWARILAQ
260 270 280 290 300
MTYYFYSFFQ ATNGKDSKKV KFVVPSGNFG DILAGYFAKK MGLPIEKLAI
310 320 330 340 350
ATNENDILDR FLKSGLYERS DKVAATLSPA MDILISSNFE RLLWYLAREY
360 370 380 390 400
LANGDDLKAG EIVNNWFQEL KTNGKFQVDK SIIEGASKDF TSERVSNEET
410 420 430 440 450
SETIKKIYES SVNPKHYILD PHTAVGVCAT ERLIAKDNDK SIQYISLSTA
460 470 480 490 500
HPAKFADAVN NALSGFSNYS FEKDVLPEEL KKLSTLKKKL KFIERADVEL
510
VKNAIEEELA KMKL
Length:514
Mass (Da):57,474
Last modified:April 1, 1990 - v1
Checksum:iEF6BA9DF56D94B6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176K → R in AAU09679 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17256 Genomic DNA. Translation: CAA35157.1.
X59720 Genomic DNA. Translation: CAA42284.1.
AY723762 Genomic DNA. Translation: AAU09679.1.
BK006937 Genomic DNA. Translation: DAA07530.1.
PIRiS22836. SYBSR.
RefSeqiNP_009982.1. NM_001178767.1.

Genome annotation databases

EnsemblFungiiCAA42284; CAA42284; CAA42284.
YCR053W; YCR053W; YCR053W.
GeneIDi850420.
KEGGisce:YCR053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17256 Genomic DNA. Translation: CAA35157.1.
X59720 Genomic DNA. Translation: CAA42284.1.
AY723762 Genomic DNA. Translation: AAU09679.1.
BK006937 Genomic DNA. Translation: DAA07530.1.
PIRiS22836. SYBSR.
RefSeqiNP_009982.1. NM_001178767.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KL7X-ray2.70A/B1-514[»]
ProteinModelPortaliP16120.
SMRiP16120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31033. 337 interactors.
DIPiDIP-6522N.
IntActiP16120. 1 interactor.
MINTiMINT-2787019.

PTM databases

iPTMnetiP16120.

Proteomic databases

MaxQBiP16120.
PRIDEiP16120.
TopDownProteomicsiP16120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42284; CAA42284; CAA42284.
YCR053W; YCR053W; YCR053W.
GeneIDi850420.
KEGGisce:YCR053W.

Organism-specific databases

EuPathDBiFungiDB:YCR053W.
SGDiS000000649. THR4.

Phylogenomic databases

GeneTreeiENSGT00530000063879.
HOGENOMiHOG000230745.
InParanoidiP16120.
KOiK01733.
OMAiEEIASWA.
OrthoDBiEOG092C1BXU.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00065.
BioCyciYEAST:YCR053W-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP16120.
PROiP16120.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR029144. Thr_synth_N.
IPR004450. Thr_synthase-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHRC_YEAST
AccessioniPrimary (citable) accession number: P16120
Secondary accession number(s): D6VR61, E9P943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 26000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.