P16117 (RPC1_BP434) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Repressor protein CI

Gene names

Name:

CI

Organism

Bacteriophage 434

Taxonomic identifier

10712 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	95 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to two sets of three contiguous operator sites in the phage genome.
Subunit structure	Homodimer, when bound to an operator.
Sequence similarities	Contains 1 HTH cro/C1-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed; by host

Chain

2 – ›95

›94

Repressor protein CI

PRO_0000149714

Regions

Domain

7 – 60

54

HTH cro/C1-type

DNA binding

18 – 37

20

H-T-H motif

Experimental info

Non-terminal residue

95

1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

95

Helix Strand Turn

Details...

Helix

3 – 13

11

Helix

18 – 25

8

Helix

29 – 36

8

Helix

46 – 52

7

Helix

57 – 62

6

Sequences

	Sequence		Length	Mass (Da)
	P16117 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6229D2DD66EA7EFC	FASTA	95	10,426
	10 20 30 40 50 60 MSISSRVKSK RIQLGLNQAE LAQKVGTTQQ SIEQLENGKT KRPRFLPELA SALGVSVDWL 70 80 90 LNGTSDSNVR FVGHVEPKGK YPLISMVRAG SWCEA

References

[1]	"Determination of vector: insert junctions in lambda gt10 cDNAs that do not recut with EcoRI. Nucleotide sequence of the lambda imm434 HindIII-EcoRI DNA fragment encoding part of the cI protein." Kuziel W.A., Tucker P.W. Nucleic Acids Res. 15:3181-3181(1987) [PubMed: 3031621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the amino-terminal domain of phage 434 repressor at 2.0-A resolution." Mondragon A., Subbiah S., Almo S.C., Drottar M., Harrison S.C. J. Mol. Biol. 205:189-200(1989) [PubMed: 2926803] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-67.
[3]	"Recognition of a DNA operator by the repressor of phage 434: a view at high resolution." Aggarwal A.K., Rodgers D.W., Drottar M., Ptashne M., Harrison S.C. Science 242:899-907(1988) [PubMed: 3187531] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-67.
[4]	"Determination of the nuclear magnetic resonance solution structure of the DNA-binding domain (residues 1 to 69) of the 434 repressor and comparison with the X-ray crystal structure." Neri D., Billeter M., Wuethrich K. J. Mol. Biol. 223:743-767(1992) [PubMed: 1311771] [Abstract] Cited for: STRUCTURE BY NMR OF 1-67.
[5]	"Structural role of a buried salt bridge in the 434 repressor DNA-binding domain." Pervushin K., Billeter M., Siegal G., Wuethrich K. J. Mol. Biol. 264:1002-1012(1996) [PubMed: 9000626] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y00118 Genomic DNA. Translation: CAA68301.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PER	X-ray	2.50	L/R	2-70	[»]
1PRA	NMR	-	A	2-70	[»]
1R63	NMR	-	A	2-64	[»]
1R69	X-ray	2.00	A	2-70	[»]
1RPD	model	-	C/D/E/F	1-95	[»]
1RPE	X-ray	2.50	L/R	2-69	[»]
1SQ8	NMR	-	A	11-62	[»]
2OR1	X-ray	2.50	L/R	2-70	[»]
2R63	NMR	-	A	2-64	[»]

ProteinModelPortal

P16117.

SMR

P16117. Positions 2-70.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001387. HTH_3.
IPR010982. Lambda_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.

Pfam

PF01381. HTH_3. 1 hit.
[Graphical view]

SMART

SM00530. HTH_XRE. 1 hit.
[Graphical view]

SUPFAM

SSF47413. Lambda_like_DNA. 1 hit.

PROSITE

PS50943. HTH_CROC1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RPC1_BP434

Accession

Primary (citable) accession number: P16117

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program