Reviewed, UniProtKB/Swiss-Prot P16116 (ALDR_BOVIN)

Names and origin

Protein names

Recommended name:
    Aldose reductase
      Short name=AR
    EC=1.1.1.21
Alternative name(s):
    Aldehyde reductase
    20-alpha-hydroxysteroid dehydrogenase
      Short name=20-alpha-HSD
    EC=1.1.1.149

Gene names

Name:

AKR1B1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Catalytic activity	Alditol + NAD(P)+ = aldose + NAD(P)H. 17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	20-alpha-hydroxysteroid dehydrogenase activity Inferred from electronic annotation. Source: EC aldehyde reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Aldose reductase		PRO_0000124622
Regions
	Nucleotide binding	9 – 18	10	NADP Potential
	Nucleotide binding	210 – 272	63	NADP By similarity
Sites
	Active site	48	1	Proton donor By similarity
	Binding site	110	1	Substrate By similarity
	Site	77	1	Lowers pKa of active site Tyr By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylalanine Ref.1
	Modified residue	22	1	Phosphoserine By similarity
	Modified residue	39	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	4	1	I → L
	Natural variant	6	1	L → I
Experimental info
	Sequence conflict	65	1	Q → K in AAA30370. Ref.2
	Sequence conflict	65	1	Q → K in AAB25333. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P16116-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 18597E7332A3F9C2	FASTA	315	35,919
	10 20 30 40 50 60 AHNIVLYTGA KMPILGLGTW KSPPGKVTEA VKVAIDLGYR HIDCAHVYQN ENEVGLALQA 70 80 90 100 110 120 KLQEQVVKRE DLFIVSKLWC TYHDKDLVKG ACQKTLSDLK LDYLDLYLIH WPTGFKPGKD 130 140 150 160 170 180 FFPLDEDGNV IPSEKDFVDT WTAMEELVDE GLVKAIGVSN FNHLQVEKIL NKPGLKYKPA 190 200 210 220 230 240 VNQIECHPYL TQEKLIQYCN SKGIVVTAYS PLGSPDRPWA KPEDPSILED PRIKAIADKY 250 260 270 280 290 300 NKTTAQVLIR FPIQRNLIVI PKSVTPERIA ENFQVFDFEL DKEDMNTLLS YNRDWRACAL 310 VSCASHRDYP FHEEF

References

[1]	"Sequence analysis of bovine lens aldose reductase." Schade S.Z., Early S.L., Williams T.R., Kezdy F.J., Heinrikson R.L., Grimshaw C.E., Doughty C.C. J. Biol. Chem. 265:3628-3635(1990) [PubMed: 2105951] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Lens.
[2]	"Molecular cloning of testicular 20 alpha-hydroxysteroid dehydrogenase: identity with aldose reductase." Warren J.C., Murdock G.L., Ma Y., Goodman S.R., Zimmer W.E. Biochemistry 32:1401-1406(1993) [PubMed: 8431420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-315. Tissue: Testis.
[3]	"Isolation and characterization of cDNA clones encoding aldose reductase." Petrash J.M., Favello A.D. Curr. Eye Res. 8:1021-1027(1989) [PubMed: 2515032] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-315. Tissue: Lens.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M31463 mRNA. Translation: AAA30370.1. Different initiation.
S54973 mRNA. Translation: AAB25333.1.

IPI

IPI00700920.

PIR

A35452.

UniGene

Bt.63116

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GO6	model	-	A	1-315	[»]

SMR

P16116. Positions 1-315.

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000009902. Bos taurus. [Contig view]

Phylogenomic databases

HOVERGEN

P16116.

Enzyme and pathway databases

BRENDA

1.1.1.149. 251.
1.1.1.21. 251.

Family and domain databases

InterPro

IPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
[Graphical view]

Gene3D

G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.

PANTHER

PTHR11732. Aldo/ket_red. 1 hit.

Pfam

PF00248. Aldo_ket_red. 1 hit.
[Graphical view]

ProDom

PD000288. Aldo/ket_red. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ALDR_BOVIN

Accession

Primary (citable) accession number: P16116

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)