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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateBy similarity
Binding sitei117 – 1171NAD or substrateBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Active sitei172 – 1721Proton acceptor
Binding sitei226 – 2261SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 3729NAD1 PublicationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
Ordered Locus Names:TM_1867
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319L-lactate dehydrogenasePRO_0000168406Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1867.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 2213Combined sources
Beta strandi26 – 316Combined sources
Helixi35 – 4814Combined sources
Helixi49 – 513Combined sources
Beta strandi56 – 594Combined sources
Helixi62 – 654Combined sources
Beta strandi69 – 735Combined sources
Helixi85 – 10622Combined sources
Beta strandi111 – 1144Combined sources
Beta strandi116 – 1183Combined sources
Helixi119 – 13012Combined sources
Turni134 – 1363Combined sources
Beta strandi137 – 1393Combined sources
Helixi143 – 15715Combined sources
Helixi161 – 1633Combined sources
Beta strandi168 – 1703Combined sources
Helixi180 – 1823Combined sources
Helixi190 – 1945Combined sources
Beta strandi197 – 1993Combined sources
Helixi202 – 22322Combined sources
Helixi228 – 24215Combined sources
Beta strandi247 – 25812Combined sources
Beta strandi263 – 27311Combined sources
Beta strandi276 – 2805Combined sources
Helixi287 – 30822Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A5ZX-ray2.10A1-319[»]
ProteinModelPortaliP16115.
SMRiP16115. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16115.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
InParanoidiP16115.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGIVGLGR VGSSTAFALL MKGFAREMVL IDVDKKRAEG DALDLIHGTP
60 70 80 90 100
FTRRANIYAG DYADLKGSDV VIVAAGVPQK PGETRLQLLG RNARVMKEIA
110 120 130 140 150
RNVSKYAPDS IVIVVTNPVD VLTYFFLKES GMDPRKVFGS GTVLDTARLR
160 170 180 190 200
TLIAQHCGFS PRSVHVYVIG EHGDSEVPVW SGAMIGGIPL QNMCQICQKC
210 220 230 240 250
DSKILENFAE KTKRAAYEII ERKGATHYAI ALAVADIVES IFFDEKRVLT
260 270 280 290 300
LSVYLEDYLG VKDLCISVPV TLGKHGVERI LELNLNEEEL EAFRKSASIL
310
KNAINEITAE ENKHQNTSG
Length:319
Mass (Da):34,994
Last modified:June 1, 1994 - v2
Checksum:iA1FB9B97CDCF290B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141Missing AA sequence (PubMed:2318202).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74302 Genomic DNA. Translation: CAA52355.1.
AE000512 Genomic DNA. Translation: AAD36929.1.
PIRiS36863.
RefSeqiNP_229663.1. NC_000853.1.
WP_004082418.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36929; AAD36929; TM_1867.
GeneIDi897800.
KEGGitma:TM1867.
PATRICi23938723. VBITheMar51294_1888.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74302 Genomic DNA. Translation: CAA52355.1.
AE000512 Genomic DNA. Translation: AAD36929.1.
PIRiS36863.
RefSeqiNP_229663.1. NC_000853.1.
WP_004082418.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A5ZX-ray2.10A1-319[»]
ProteinModelPortaliP16115.
SMRiP16115. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1867.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36929; AAD36929; TM_1867.
GeneIDi897800.
KEGGitma:TM1867.
PATRICi23938723. VBITheMar51294_1888.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
InParanoidiP16115.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Miscellaneous databases

EvolutionaryTraceiP16115.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The L-lactate dehydrogenase gene of the hyperthermophilic bacterium Thermotoga maritima cloned by complementation in Escherichia coli."
    Ostendorp R., Liebl W., Schurig H., Jaenicke R.
    Eur. J. Biochem. 216:709-715(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Lactate dehydrogenase from the extreme thermophile Thermotoga maritima."
    Wrba A., Jaenicke R., Huber R., Stetter K.O.
    Eur. J. Biochem. 188:195-201(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-31.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  4. "Lactate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: the crystal structure at 2.1-A resolution reveals strategies for intrinsic protein stabilization."
    Auerbach G., Ostendorp R., Prade L., Korndorfer I., Dams T., Huber R., Jaenicke R.
    Structure 6:769-781(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH NAD.

Entry informationi

Entry nameiLDH_THEMA
AccessioniPrimary (citable) accession number: P16115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: April 13, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.