Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85SubstrateBy similarity1
Binding sitei117NAD or substrateBy similarity1
Binding sitei148SubstrateBy similarity1
Active sitei172Proton acceptor1
Binding sitei226SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 37NAD1 PublicationAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
Ordered Locus Names:TM_1867
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001684061 – 319L-lactate dehydrogenaseAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei217PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1867.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 22Combined sources13
Beta strandi26 – 31Combined sources6
Helixi35 – 48Combined sources14
Helixi49 – 51Combined sources3
Beta strandi56 – 59Combined sources4
Helixi62 – 65Combined sources4
Beta strandi69 – 73Combined sources5
Helixi85 – 106Combined sources22
Beta strandi111 – 114Combined sources4
Beta strandi116 – 118Combined sources3
Helixi119 – 130Combined sources12
Turni134 – 136Combined sources3
Beta strandi137 – 139Combined sources3
Helixi143 – 157Combined sources15
Helixi161 – 163Combined sources3
Beta strandi168 – 170Combined sources3
Helixi180 – 182Combined sources3
Helixi190 – 194Combined sources5
Beta strandi197 – 199Combined sources3
Helixi202 – 223Combined sources22
Helixi228 – 242Combined sources15
Beta strandi247 – 258Combined sources12
Beta strandi263 – 273Combined sources11
Beta strandi276 – 280Combined sources5
Helixi287 – 308Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A5ZX-ray2.10A1-319[»]
ProteinModelPortaliP16115.
SMRiP16115.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16115.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
InParanoidiP16115.
KOiK00016.
OMAiNNEHRVL.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGIVGLGR VGSSTAFALL MKGFAREMVL IDVDKKRAEG DALDLIHGTP
60 70 80 90 100
FTRRANIYAG DYADLKGSDV VIVAAGVPQK PGETRLQLLG RNARVMKEIA
110 120 130 140 150
RNVSKYAPDS IVIVVTNPVD VLTYFFLKES GMDPRKVFGS GTVLDTARLR
160 170 180 190 200
TLIAQHCGFS PRSVHVYVIG EHGDSEVPVW SGAMIGGIPL QNMCQICQKC
210 220 230 240 250
DSKILENFAE KTKRAAYEII ERKGATHYAI ALAVADIVES IFFDEKRVLT
260 270 280 290 300
LSVYLEDYLG VKDLCISVPV TLGKHGVERI LELNLNEEEL EAFRKSASIL
310
KNAINEITAE ENKHQNTSG
Length:319
Mass (Da):34,994
Last modified:June 1, 1994 - v2
Checksum:iA1FB9B97CDCF290B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14Missing AA sequence (PubMed:2318202).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74302 Genomic DNA. Translation: CAA52355.1.
AE000512 Genomic DNA. Translation: AAD36929.1.
PIRiS36863.
RefSeqiNP_229663.1. NC_000853.1.
WP_004082418.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36929; AAD36929; TM_1867.
GeneIDi897800.
KEGGitma:TM1867.
PATRICi23938723. VBITheMar51294_1888.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74302 Genomic DNA. Translation: CAA52355.1.
AE000512 Genomic DNA. Translation: AAD36929.1.
PIRiS36863.
RefSeqiNP_229663.1. NC_000853.1.
WP_004082418.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A5ZX-ray2.10A1-319[»]
ProteinModelPortaliP16115.
SMRiP16115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1867.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36929; AAD36929; TM_1867.
GeneIDi897800.
KEGGitma:TM1867.
PATRICi23938723. VBITheMar51294_1888.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
InParanoidiP16115.
KOiK00016.
OMAiNNEHRVL.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Miscellaneous databases

EvolutionaryTraceiP16115.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_THEMA
AccessioniPrimary (citable) accession number: P16115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.