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Protein

Galectin-3

Gene

Lgals3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIgE-binding protein
Biological processDifferentiation, Immunity, Innate immunity, mRNA processing, mRNA splicing
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-3000471. Scavenging by Class B Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-3
Short name:
Gal-3
Alternative name(s):
35 kDa lectin
Carbohydrate-binding protein 35
Short name:
CBP 35
Galactose-specific lectin 3
IgE-binding protein
L-34 galactoside-binding lectin
Laminin-binding protein
Lectin L-29
Mac-2 antigen
Gene namesi
Name:Lgals3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96778. Lgals3.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: CACAO
  • cytoplasm Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular region Source: Reactome
  • extracellular space Source: BHF-UCL
  • glial cell projection Source: MGI
  • immunological synapse Source: BHF-UCL
  • membrane Source: MGI
  • mitochondrial inner membrane Source: MGI
  • nucleus Source: MGI
  • proteinaceous extracellular matrix Source: MGI
  • spliceosomal complex Source: UniProtKB-KW

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted, Spliceosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3668.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000769312 – 264Galectin-3Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei6Phosphoserine; by CK1By similarity1
Disulfide bondi187Interchain1 Publication
Modified residuei202PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP16110.
MaxQBiP16110.
PaxDbiP16110.
PeptideAtlasiP16110.
PRIDEiP16110.

PTM databases

iPTMnetiP16110.
PhosphoSitePlusiP16110.

Expressioni

Tissue specificityi

The highest levels are found in activated macrophages.

Gene expression databases

CleanExiMM_LGALS3.

Interactioni

Subunit structurei

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SufuQ9Z0P75EBI-3508325,EBI-3508336

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-2152N.
IntActiP16110. 4 interactors.
STRINGi10090.ENSMUSP00000114350.

Chemistry databases

BindingDBiP16110.

Structurei

3D structure databases

ProteinModelPortaliP16110.
SMRiP16110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati35 – 4319
Repeati44 – 5229
Repeati53 – 6139
Repeati62 – 7049
Repeati71 – 7959
Repeati80 – 8869
Repeati89 – 9779
Repeati98 – 107810
Repeati108 – 1149; truncated7
Domaini132 – 262GalectinPROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 1149 X 9 AA tandem repeats of Y-P-G-X(3)-P-[GS]-AAdd BLAST80
Regioni195 – 201Beta-galactoside bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi240 – 255Nuclear export signalAdd BLAST16

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000246423.
HOVERGENiHBG006255.
InParanoidiP16110.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR015534. Galectin_3.
IPR001079. Galectin_CRD.
PANTHERiPTHR11346:SF145. PTHR11346:SF145. 1 hit.
PfamiView protein in Pfam
PF00337. Gal-bind_lectin. 1 hit.
SMARTiView protein in SMART
SM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS51304. GALECTIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSFSLNDA LAGSGNPNPQ GYPGAWGNQP GAGGYPGAAY PGAYPGQAPP
60 70 80 90 100
GAYPGQAPPG AYPGQAPPSA YPGPTAPGAY PGPTAPGAYP GQPAPGAFPG
110 120 130 140 150
QPGAPGAYPQ CSGGYPAAGP YGVPAGPLTV PYDLPLPGGV MPRMLITIMG
160 170 180 190 200
TVKPNANRIV LDFRRGNDVA FHFNPRFNEN NRRVIVCNTK QDNNWGKEER
210 220 230 240 250
QSAFPFESGK PFKIQVLVEA DHFKVAVNDA HLLQYNHRMK NLREISQLGI
260
SGDITLTSAN HAMI
Length:264
Mass (Da):27,515
Last modified:January 23, 2007 - v3
Checksum:i1B5A8A81093D68F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → R in AAA37311 (PubMed:3360772).Curated1
Sequence conflicti4S → T in CAA34206 (PubMed:2525069).Curated1
Sequence conflicti92 – 93QP → ST in CAA34206 (PubMed:2525069).Curated2
Sequence conflicti110 – 112QCS → SAP in CAA34206 (PubMed:2525069).Curated3
Sequence conflicti252G → R in AAA37311 (PubMed:3360772).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16834 mRNA. Translation: CAA34736.1.
J03723 mRNA. Translation: AAA37311.1.
X16074 mRNA. Translation: CAA34206.1.
CCDSiCCDS26986.1.
PIRiS08537. A28651.
UniGeneiMm.248615.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLEG3_MOUSE
AccessioniPrimary (citable) accession number: P16110
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot