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Protein

Galectin-3

Gene

Lgals3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation, Immunity, Innate immunity, mRNA processing, mRNA splicing

Keywords - Ligandi

IgE-binding protein, Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-3
Short name:
Gal-3
Alternative name(s):
35 kDa lectin
Carbohydrate-binding protein 35
Short name:
CBP 35
Galactose-specific lectin 3
IgE-binding protein
L-34 galactoside-binding lectin
Laminin-binding protein
Lectin L-29
Mac-2 antigen
Gene namesi
Name:Lgals3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96778. Lgals3.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: CACAO
  • cytoplasm Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • immunological synapse Source: BHF-UCL
  • membrane Source: MGI
  • mitochondrial inner membrane Source: MGI
  • nucleus Source: MGI
  • proteinaceous extracellular matrix Source: MGI
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted, Spliceosome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3668.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 264263Galectin-3PRO_0000076931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei6 – 61Phosphoserine; by CK1By similarity
Disulfide bondi187 – 187Interchain1 Publication
Modified residuei202 – 2021PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP16110.
MaxQBiP16110.
PaxDbiP16110.
PeptideAtlasiP16110.
PRIDEiP16110.

PTM databases

iPTMnetiP16110.
PhosphoSiteiP16110.

Expressioni

Tissue specificityi

The highest levels are found in activated macrophages.

Gene expression databases

CleanExiMM_LGALS3.

Interactioni

Subunit structurei

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SufuQ9Z0P75EBI-3508325,EBI-3508336

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-2152N.
IntActiP16110. 4 interactions.
STRINGi10090.ENSMUSP00000114350.

Chemistry

BindingDBiP16110.

Structurei

3D structure databases

ProteinModelPortaliP16110.
SMRiP16110. Positions 127-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati35 – 4391
Repeati44 – 5292
Repeati53 – 6193
Repeati62 – 7094
Repeati71 – 7995
Repeati80 – 8896
Repeati89 – 9797
Repeati98 – 107108
Repeati108 – 11479; truncated
Domaini132 – 262131GalectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 114809 X 9 AA tandem repeats of Y-P-G-X(3)-P-[GS]-AAdd
BLAST
Regioni195 – 2017Beta-galactoside bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi240 – 25516Nuclear export signalAdd
BLAST

Sequence similaritiesi

Contains 1 galectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000246423.
HOVERGENiHBG006255.
InParanoidiP16110.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR015534. Galectin_3.
IPR001079. Galectin_CRD.
[Graphical view]
PANTHERiPTHR11346:SF26. PTHR11346:SF26. 1 hit.
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSFSLNDA LAGSGNPNPQ GYPGAWGNQP GAGGYPGAAY PGAYPGQAPP
60 70 80 90 100
GAYPGQAPPG AYPGQAPPSA YPGPTAPGAY PGPTAPGAYP GQPAPGAFPG
110 120 130 140 150
QPGAPGAYPQ CSGGYPAAGP YGVPAGPLTV PYDLPLPGGV MPRMLITIMG
160 170 180 190 200
TVKPNANRIV LDFRRGNDVA FHFNPRFNEN NRRVIVCNTK QDNNWGKEER
210 220 230 240 250
QSAFPFESGK PFKIQVLVEA DHFKVAVNDA HLLQYNHRMK NLREISQLGI
260
SGDITLTSAN HAMI
Length:264
Mass (Da):27,515
Last modified:January 23, 2007 - v3
Checksum:i1B5A8A81093D68F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → R in AAA37311 (PubMed:3360772).Curated
Sequence conflicti4 – 41S → T in CAA34206 (PubMed:2525069).Curated
Sequence conflicti92 – 932QP → ST in CAA34206 (PubMed:2525069).Curated
Sequence conflicti110 – 1123QCS → SAP in CAA34206 (PubMed:2525069).Curated
Sequence conflicti252 – 2521G → R in AAA37311 (PubMed:3360772).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16834 mRNA. Translation: CAA34736.1.
J03723 mRNA. Translation: AAA37311.1.
X16074 mRNA. Translation: CAA34206.1.
CCDSiCCDS26986.1.
PIRiS08537. A28651.
UniGeneiMm.248615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16834 mRNA. Translation: CAA34736.1.
J03723 mRNA. Translation: AAA37311.1.
X16074 mRNA. Translation: CAA34206.1.
CCDSiCCDS26986.1.
PIRiS08537. A28651.
UniGeneiMm.248615.

3D structure databases

ProteinModelPortaliP16110.
SMRiP16110. Positions 127-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-2152N.
IntActiP16110. 4 interactions.
STRINGi10090.ENSMUSP00000114350.

Chemistry

BindingDBiP16110.
ChEMBLiCHEMBL3668.

PTM databases

iPTMnetiP16110.
PhosphoSiteiP16110.

Proteomic databases

EPDiP16110.
MaxQBiP16110.
PaxDbiP16110.
PeptideAtlasiP16110.
PRIDEiP16110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96778. Lgals3.

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000246423.
HOVERGENiHBG006255.
InParanoidiP16110.

Miscellaneous databases

ChiTaRSiLgals3. mouse.
PROiP16110.
SOURCEiSearch...

Gene expression databases

CleanExiMM_LGALS3.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR015534. Galectin_3.
IPR001079. Galectin_CRD.
[Graphical view]
PANTHERiPTHR11346:SF26. PTHR11346:SF26. 1 hit.
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEG3_MOUSE
AccessioniPrimary (citable) accession number: P16110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.