P16096 (ALFC_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Fructose-bisphosphate aldolase, chloroplastic EC=4.1.2.13
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Chloroplast Ref.2
	Chain	47 – 394	348	Fructose-bisphosphate aldolase, chloroplastic		PRO_0000001113
Sites
	Active site	223	1	Proton acceptor By similarity
	Active site	265	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Site	394	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Experimental info
	Sequence conflict	260 – 263	4	EGSS → RDP in CAA47293. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16096 [UniParc]. Last modified November 1, 1995. Version 3. Checksum: 9D8A813E1636B274	FASTA	394	42,468
	10 20 30 40 50 60 MASASLLKTS PVLDNPEFLK GQTLRIPSVA GVRFTPSGSS SLTVRASSYA DELVKTAKTV 70 80 90 100 110 120 ASPGRGILAM DESNATCGKR LASIGLENTE ANRQAYRTLL ISAPGLGQYV SGAILFEETL 130 140 150 160 170 180 YQSTTDGKKM VDVLIEQGIV PGIKVDKGWL PLPGSNDESW CQGLDGLACR SAAYYQQGAR 190 200 210 220 230 240 FAKWRTVVSI PNGPSALAVK EAAWGLARYA AITQDNGLDP ILEPEIMLDG EHGIDRTFRV 250 260 270 280 290 300 AQQVWAEVFF NLAENNVLLE GSSLKPSMVG PGALSARKGP PEQVADYPLK LLHRRRGPVV 310 320 330 340 350 360 PGIMVLSGGQ SEVEATLNLN AMNQSPNPWH VSFSYARALQ NTCLKTWVEG QENVKAQDFA 370 380 390 CAKSNSLAQL GKYTGEGESE ERKKDMFVKA TLTY

References

[1]	"Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach." Pelzer-Reith B., Penger A., Schnarrenberger C. Plant Mol. Biol. 21:331-340(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and characterization of the cytosolic and chloroplast forms of spinach leaf fructose diphosphate aldolase." Lebherz H.G., Leadbetter M.M., Bradshaw R.A. J. Biol. Chem. 259:1011-1017(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 47-64.
[3]	Bairoch A. Unpublished observations (NOV-1995) Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66814 mRNA. Translation: CAA47293.1.
PIR	ADSPAP. S31090.
3D structure databases
ProteinModelPortal	P16096.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P16096.
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC_SPIOL

Accession

Primary (citable) accession number: P16096

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program