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P16096

- ALFC_SPIOL

UniProt

P16096 - ALFC_SPIOL

Protein

Fructose-bisphosphate aldolase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 3 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Active sitei223 – 2231Proton acceptorBy similarity
    Active sitei265 – 2651Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Sitei394 – 3941Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12899.
    SABIO-RKP16096.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase, chloroplastic (EC:4.1.2.13)
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4646Chloroplast1 PublicationAdd
    BLAST
    Chaini47 – 394348Fructose-bisphosphate aldolase, chloroplasticPRO_0000001113Add
    BLAST

    Proteomic databases

    PRIDEiP16096.

    Structurei

    3D structure databases

    ProteinModelPortaliP16096.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16096-1 [UniParc]FASTAAdd to Basket

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    MASASLLKTS PVLDNPEFLK GQTLRIPSVA GVRFTPSGSS SLTVRASSYA    50
    DELVKTAKTV ASPGRGILAM DESNATCGKR LASIGLENTE ANRQAYRTLL 100
    ISAPGLGQYV SGAILFEETL YQSTTDGKKM VDVLIEQGIV PGIKVDKGWL 150
    PLPGSNDESW CQGLDGLACR SAAYYQQGAR FAKWRTVVSI PNGPSALAVK 200
    EAAWGLARYA AITQDNGLDP ILEPEIMLDG EHGIDRTFRV AQQVWAEVFF 250
    NLAENNVLLE GSSLKPSMVG PGALSARKGP PEQVADYPLK LLHRRRGPVV 300
    PGIMVLSGGQ SEVEATLNLN AMNQSPNPWH VSFSYARALQ NTCLKTWVEG 350
    QENVKAQDFA CAKSNSLAQL GKYTGEGESE ERKKDMFVKA TLTY 394
    Length:394
    Mass (Da):42,468
    Last modified:November 1, 1995 - v3
    Checksum:i9D8A813E1636B274
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti260 – 2634EGSS → RDP in CAA47293. (PubMed:8425060)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66814 mRNA. Translation: CAA47293.1.
    PIRiS31090. ADSPAP.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66814 mRNA. Translation: CAA47293.1 .
    PIRi S31090. ADSPAP.

    3D structure databases

    ProteinModelPortali P16096.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P16096.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    BioCyci MetaCyc:MONOMER-12899.
    SABIO-RK P16096.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach."
      Pelzer-Reith B., Penger A., Schnarrenberger C.
      Plant Mol. Biol. 21:331-340(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and characterization of the cytosolic and chloroplast forms of spinach leaf fructose diphosphate aldolase."
      Lebherz H.G., Leadbetter M.M., Bradshaw R.A.
      J. Biol. Chem. 259:1011-1017(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 47-64.
    3. Bairoch A.
      Unpublished observations (NOV-1995)
      Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

    Entry informationi

    Entry nameiALFC_SPIOL
    AccessioniPrimary (citable) accession number: P16096
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 86 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3