Reviewed, UniProtKB/Swiss-Prot P16093 (RIPK_TRIKI)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein trichokirin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Trichosanthes kirilowii (Mongolian snake-gourd)
Taxonomic identifier	3677 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Trichosanthes

Protein attributes

Sequence length	16 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Post-translational modification	Glycosylated.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›16	›16	Ribosome-inactivating protein trichokirin		PRO_0000221408
Experimental info
	Non-terminal residue	16	1

Sequences

	Sequence		Length	Mass (Da)
	P16093-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 5E268A7F345935A2	FASTA	16	1,605
	10 DVSFSLSGGG TASYEK

References

[1]

"Trichokirin, a ribosome-inactivating protein from the seeds of Trichosanthes kirilowii Maximowicz. Purification, partial characterization and use for preparation of immunotoxins." Casellas P., Dussossoy D., Falasca A.I., Barbieri L., Guillemot J.-C., Ferrara P., Bolognesi A., Cenini P., Stirpe F. Eur. J. Biochem. 176:581-588(1988) [PubMed: 3262509] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.

Cross-references

Sequence databases
PIR	S01669.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273574.
Family and domain databases
InterPro	IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPK_TRIKI

Accession

Primary (citable) accession number: P16093

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 5, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)