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Protein

Gag polyprotein

Gene

gag

Organism
Feline immunodeficiency virus (isolate Petaluma) (FIV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane.By similarity
Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p13 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri375 – 392CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri394 – 411CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virion maturation, Virus exit from host cell

Keywords - Ligandi

Metal-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Capsid protein p24
Short name:
CA
Gene namesi
Name:gag
OrganismiFeline immunodeficiency virus (isolate Petaluma) (FIV)
Taxonomic identifieri11674 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
Virus hostiFelidae (cat family) [TaxID: 9681]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000387841 – 135Matrix protein p15Add BLAST135
ChainiPRO_0000038785136 – 357Capsid protein p24Add BLAST222
PeptideiPRO_0000272316358 – 366p1Sequence analysis9
ChainiPRO_0000038786367 – 450Nucleocapsid protein p13Add BLAST84

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi7 – 16Combined sources10
Beta strandi19 – 26Combined sources8
Helixi32 – 45Combined sources14
Beta strandi49 – 51Combined sources3
Helixi57 – 74Combined sources18
Helixi78 – 93Combined sources16
Helixi103 – 112Combined sources10
Turni113 – 116Combined sources4
Helixi121 – 124Combined sources4
Helixi288 – 301Combined sources14
Helixi306 – 319Combined sources14
Helixi323 – 328Combined sources6
Turni329 – 331Combined sources3
Helixi338 – 345Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2N1RNMR-A2-135[»]
4IC9X-ray2.00A1-135[»]
4ICAX-ray2.70A/B1-120[»]
5DCKX-ray2.29A/B278-347[»]
ProteinModelPortaliP16087.
SMRiP16087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi438 – 441PTAP/PSAP motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Nucleocapsid protein p13 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 (By similarity).By similarity

Sequence similaritiesi

Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri375 – 392CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri394 – 411CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNGQGRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP
60 70 80 90 100
GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV
110 120 130 140 150
STAAAAENMY SQMGLDTRPS MKEAGGKEEG PPQAYPIQTV NGVPQYVALD
160 170 180 190 200
PKMVSIFMEK AREGLGGEEV QLWFTAFSAN LTPTDMATLI MAAPGCAADK
210 220 230 240 250
EILDESLKQL TAEYDRTHPP DAPRPLPYFT AAEIMGIGLT QEQQAEARFA
260 270 280 290 300
PARMQCRAWY LEALGKLAAI KAKSPRAVQL RQGAKEDYSS FIDRLFAQID
310 320 330 340 350
QEQNTAEVKL YLKQSLSIAN ANADCKKAMS HLKPESTLEE KLRACQEIGS
360 370 380 390 400
PGYKMQLLAE ALTKVQVVQS KGSGPVCFNC KKPGHLARQC REVKKCNKCG
410 420 430 440 450
KPGHLAAKCW QGNRKNSGNW KAGRAAAPVN QMQQAVMPSA PPMEEKLLDL
Length:450
Mass (Da):49,244
Last modified:April 1, 1990 - v1
Checksum:iE10A98DEB9494B94
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405L → V in AAB59936 (PubMed:2762293).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25381 Genomic RNA. Translation: AAB59936.1.
PIRiA33543. FOLJFP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25381 Genomic RNA. Translation: AAB59936.1.
PIRiA33543. FOLJFP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2N1RNMR-A2-135[»]
4IC9X-ray2.00A1-135[»]
4ICAX-ray2.70A/B1-120[»]
5DCKX-ray2.29A/B278-347[»]
ProteinModelPortaliP16087.
SMRiP16087.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAG_FIVPE
AccessioniPrimary (citable) accession number: P16087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.