P16087 (GAG_FIVPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gag polyprotein

Cleaved into the following 4 chains:

1. Matrix protein p15
   Short name=MA
2. Capsid protein p24
   Short name=CA
3. p1
4. Nucleocapsid protein p13
   Short name=NC

Gene names

Name:

gag

Organism

Feline immunodeficiency virus (isolate Petaluma) (FIV)

Taxonomic identifier

11674 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Feline lentivirus group › Feline immunodeficiency virus

Virus host

Felidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane By similarity. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity. Nucleocapsid protein p13 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers By similarity.
Subcellular location	Matrix protein p15: Virion Potential. Capsid protein p24: Virion Potential. Nucleocapsid protein p13: Virion Potential.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Nucleocapsid protein p13 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 By similarity.
Sequence similarities	Belongs to the feline lentivirus group gag polyprotein family. Contains 2 CCHC-type zinc fingers.

Ontologies

Keywords
Biological process	Capsid maturation Host-virus interaction Viral budding Viral budding via the host ESCRT complexes Virus exit from host cell
Cellular component	Viral matrix protein Virion
Domain	Repeat Zinc-finger
Ligand	Metal-binding Viral nucleoprotein Zinc
Molecular function	Capsid protein
Gene Ontology (GO)
Biological_process	viral procapsid maturation Inferred from electronic annotation. Source: UniProtKB-KW viral release from host cell Inferred from electronic annotation. Source: UniProtKB-KW virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	Matrix protein p15		PRO_0000038784
	Chain	136 – 357	222	Capsid protein p24		PRO_0000038785
	Peptide	358 – 366	9	p1 Potential		PRO_0000272316
	Chain	367 – 450	84	Nucleocapsid protein p13		PRO_0000038786
Regions
	Zinc finger	375 – 392	18	CCHC-type 1
	Zinc finger	394 – 411	18	CCHC-type 2
	Motif	438 – 441	4	PTAP/PSAP motif
Experimental info
	Sequence conflict	405	1	L → V in AAB59936. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16087 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E10A98DEB9494B94	FASTA	450	49,244
	10 20 30 40 50 60 MGNGQGRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL 70 80 90 100 110 120 RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPS 130 140 150 160 170 180 MKEAGGKEEG PPQAYPIQTV NGVPQYVALD PKMVSIFMEK AREGLGGEEV QLWFTAFSAN 190 200 210 220 230 240 LTPTDMATLI MAAPGCAADK EILDESLKQL TAEYDRTHPP DAPRPLPYFT AAEIMGIGLT 250 260 270 280 290 300 QEQQAEARFA PARMQCRAWY LEALGKLAAI KAKSPRAVQL RQGAKEDYSS FIDRLFAQID 310 320 330 340 350 360 QEQNTAEVKL YLKQSLSIAN ANADCKKAMS HLKPESTLEE KLRACQEIGS PGYKMQLLAE 370 380 390 400 410 420 ALTKVQVVQS KGSGPVCFNC KKPGHLARQC REVKKCNKCG KPGHLAAKCW QGNRKNSGNW 430 440 450 KAGRAAAPVN QMQQAVMPSA PPMEEKLLDL

References

[1]	"Nucleotide sequence and genomic organization of feline immunodeficiency virus." Talbott R.L., Sparger E.E., Lovelace K.M., Fitch W.M., Pedersen N.C., Luciw P.A., Elder J.H. Proc. Natl. Acad. Sci. U.S.A. 86:5743-5747(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Clone 34TF10.
[2]	"Nucleotide sequence analysis of feline immunodeficiency virus: genome organization and relationship to other lentiviruses." Olmsted R.A., Hirsch V.M., Purcell R.H., Johnson P.R. Proc. Natl. Acad. Sci. U.S.A. 86:8088-8092(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Clone FIV-14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25381 Genomic RNA. Translation: AAB59936.1.
PIR	FOLJFP. A33543.
3D structure databases
ProteinModelPortal	P16087.
SMR	P16087. Positions 376-409.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1200.30. 1 hit. 1.10.375.10. 1 hit. 4.10.60.10. 1 hit.
InterPro	IPR000721. Gag_p24. IPR008916. Retrov_capsid_C. IPR008919. Retrov_capsid_N. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF00607. Gag_p24. 1 hit. PF00098. zf-CCHC. 2 hits. [Graphical view]
SMART	SM00343. ZnF_C2HC. 2 hits. [Graphical view]
SUPFAM	SSF47353. Retrov_capsid_C. 1 hit. SSF47943. Retrov_capsid_N. 1 hit. SSF57756. SSF57756. 1 hit.
PROSITE	PS50158. ZF_CCHC. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAG_FIVPE

Accession

Primary (citable) accession number: P16087

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program