P16071 (HN_PI1HW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18

Gene names

Name:

HN

Organism

Human parainfluenza 1 virus (strain Washington/1957) (HPIV-1)

Taxonomic identifier

11211 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus › Human parainfluenza virus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host cell membrane Host membrane Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Hemagglutinin-neuraminidase		PRO_0000142621
Regions
	Topological domain	1 – 34	34	Intravirion Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Topological domain	56 – 575	520	Virion surface Potential
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	499	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	511	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P16071 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 63E55E481A0DB322	FASTA	575	63,981
	10 20 30 40 50 60 MAEKGKTNSS YWSTTRNDNS TVNTYIDTPA GKTHIWLLIA TTMHTILSFI IMILCIDLII 70 80 90 100 110 120 KQDTCMKTNI MTVSSMNESA KTIKETITEL IRQEVISRTI NIQSSVQSGI PILLNKQSRD 130 140 150 160 170 180 LTQLIEKSCN RQELAQICEN TIAIHHADGI SPLDPHDFWR CPVGEPLLSN NPNISLLPGP 190 200 210 220 230 240 SLLSGSTTIS GCVRLPSLSI GDAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMYPD 250 260 270 280 290 300 LKPVISHTYD INDNRKSCSV IAAGTRGYQL CSLPTVNETT DYSSEGIEDL VFDILDLKGK 310 320 330 340 350 360 TKSHRYKNED ITFDHPFSAM YPSVGSGIKI ENTLIFLGYG GLTTPLQGDT KCVTNRCANV 370 380 390 400 410 420 NQSVCNDALK ITWLKKRQVV NVLIRINNYL SDRPKIVVET IPITQNYLGA EGRLLKLGKK 430 440 450 460 470 480 IYIYTRSSGW HSHLQIGSLD INNPMTIKWA PHEVLSRPGN QDCNWYNRCP RECISGVYTD 490 500 510 520 530 540 AYPLSPDAVN VATTTLYANT SRVNPTIMYS NTSEIINMLR LKNVQLEAAY TTTSCITHFG 550 560 570 KGYCFHIVEI NQTSLNTLQP MLFKTSIPKI CKITS

References

[1]

"The hemagglutinin-neuraminidase glycoproteins of human parainfluenza virus type 1 and Sendai virus have high structure-function similarity with limited antigenic cross-reactivity." Gorman W.L., Gill D.S., Scroggs R.A., Portner A. Virology 175:211-221(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31228 Genomic RNA. Translation: AAA46798.1.
PIR	HNNZ39. A34682.
3D structure databases
ProteinModelPortal	P16071.
SMR	P16071. Positions 144-572.
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.120.10.10. 1 hit.
InterPro	IPR000665. Hemagglutn-neuramid. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Sialidases. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_PI1HW

Accession

Primary (citable) accession number: P16071

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program