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Protein

Guanylate cyclase soluble subunit beta-1

Gene

GUCY1B1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.1 Publication

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.1 Publication

Cofactori

heme2 PublicationsNote: Binds 1 or 2 heme groups per heterodimer. Heme is required for responding to nitric oxide, but not for catalytic activity.2 Publications

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions, binding of NO to the heme iron increases catalytic activity up to 400 folds.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron (heme proximal ligand)2 Publications1

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: UniProtKB
  • heme binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to nitric oxide Source: UniProtKB
  • cGMP biosynthetic process Source: UniProtKB
  • nitric oxide-cGMP-mediated signaling pathway Source: UniProtKB

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis
LigandGTP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2 908

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit beta-1 (EC:4.6.1.21 Publication)
Short name:
GCS-beta-1
Alternative name(s):
Guanylate cyclase soluble subunit beta-3
Short name:
GCS-beta-3
Soluble guanylate cyclase small subunit
Gene namesi
Name:GUCY1B1
Synonyms:GUC1B3, GUCY1B3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 17

Organism-specific databases

VGNCiVGNC:29721 GUCY1B1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105H → A or G: Abolishes heme binding. 1 Publication1
Mutagenesisi105H → F: Does not affect basal levels of enzyme activity, but abolishes stimulation by nitric oxide. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741151 – 619Guanylate cyclase soluble subunit beta-1Add BLAST619

Proteomic databases

PaxDbiP16068
PRIDEiP16068

Expressioni

Tissue specificityi

Lung and brain.

Gene expression databases

BgeeiENSBTAG00000003840
ExpressionAtlasiP16068 baseline and differential

Interactioni

Subunit structurei

The active enzyme is formed by a heterodimer of an alpha and a beta subunit (PubMed:7908439, PubMed:9521770). Heterodimer with GUCY1A1. Can also form inactive homodimers in vitro.By similarity2 Publications

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055570

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AWNmodel-B412-572[»]
ProteinModelPortaliP16068
SMRiP16068
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini421 – 554Guanylate cyclasePROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000220903
HOVERGENiHBG051715
InParanoidiP16068
KOiK12319
OMAiEFLCEAP
OrthoDBiEOG091G07GF

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
3.90.1520.10, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR038158 H-NOX_domain_sf
IPR011644 Heme_NO-bd
IPR011645 HNOB_dom_associated
IPR024096 NO_sig/Golgi_transp_ligand-bd
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 1 hit
PF07700 HNOB, 1 hit
PF07701 HNOBA, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF111126 SSF111126, 1 hit
SSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P16068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD
60 70 80 90 100
LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN
110 120 130 140 150
LDALHDHLAT IYPGMRAPSF RCTDADKGKG LILHYYSERE GLQDIVIGII
160 170 180 190 200
KTVAQQIHGT EIDMKVIQQR NEECDHTQFL IEEKESKEED FYEDLDRFEE
210 220 230 240 250
NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL PQLQPGNCSL
260 270 280 290 300
LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKS ECEDELTGTE
310 320 330 340 350
ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR
360 370 380 390 400
DLVLLGEQFR EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP
410 420 430 440 450
SVANELRHKR PVPAKRYDNV TILFSGIVGF NAFCSKHASG EGAMKIVNLL
460 470 480 490 500
NDLYTRFDTL TDSRKNPFVY KVETVGDKYM TVSGLPEPCI HHARSICHLA
510 520 530 540 550
LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY CLFGNTVNLT
560 570 580 590 600
SRTETTGEKG KINVSEYTYR CLMTPENSDP QFHLEHRGPV SMKGKKEPMQ
610
VWFLSRKNTG TEETEQDEN
Length:619
Mass (Da):70,502
Last modified:April 1, 1990 - v1
Checksum:i8EFB14952B80F344
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00770 mRNA Translation: CAA68739.1
BC133308 mRNA Translation: AAI33309.1
PIRiS01653 OYBO70
RefSeqiNP_777066.1, NM_174641.1
UniGeneiBt.109786

Genome annotation databases

EnsembliENSBTAT00000005009; ENSBTAP00000005009; ENSBTAG00000003840
GeneIDi282433
KEGGibta:282433

Similar proteinsi

Entry informationi

Entry nameiGCYB1_BOVIN
AccessioniPrimary (citable) accession number: P16068
Secondary accession number(s): A2VDM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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