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P16068 (GCYB1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanylate cyclase soluble subunit beta-1

Short name=GCS-beta-1
EC=4.6.1.2
Alternative name(s):
Guanylate cyclase soluble subunit beta-3
Short name=GCS-beta-3
Soluble guanylate cyclase small subunit
Gene names
Name:GUCY1B1
Synonyms:GUC1B3, GUCY1B3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

GTP = 3',5'-cyclic GMP + diphosphate.

Cofactor

Binds 1 or 2 heme groups per heterodimer.

Enzyme regulation

Activated by nitric oxide in the presence of magnesium or manganese ions, binding of NO to the heme iron increases catalytic activity up to 400 folds.

Subunit structure

Heterodimer of an alpha and a beta chain.

Subcellular location

Cytoplasm.

Tissue specificity

Lung and brain.

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 1 guanylate cyclase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Guanylate cyclase soluble subunit beta-1
PRO_0000074115

Regions

Domain421 – 554134Guanylate cyclase

Sites

Metal binding1051Iron (heme proximal ligand)

Secondary structure

......................... 619
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16068 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 8EFB14952B80F344

FASTA61970,502
        10         20         30         40         50         60 
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN 

        70         80         90        100        110        120 
LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN LDALHDHLAT IYPGMRAPSF 

       130        140        150        160        170        180 
RCTDADKGKG LILHYYSERE GLQDIVIGII KTVAQQIHGT EIDMKVIQQR NEECDHTQFL 

       190        200        210        220        230        240 
IEEKESKEED FYEDLDRFEE NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL 

       250        260        270        280        290        300 
PQLQPGNCSL LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKS ECEDELTGTE 

       310        320        330        340        350        360 
ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR DLVLLGEQFR 

       370        380        390        400        410        420 
EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP SVANELRHKR PVPAKRYDNV 

       430        440        450        460        470        480 
TILFSGIVGF NAFCSKHASG EGAMKIVNLL NDLYTRFDTL TDSRKNPFVY KVETVGDKYM 

       490        500        510        520        530        540 
TVSGLPEPCI HHARSICHLA LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY 

       550        560        570        580        590        600 
CLFGNTVNLT SRTETTGEKG KINVSEYTYR CLMTPENSDP QFHLEHRGPV SMKGKKEPMQ 

       610 
VWFLSRKNTG TEETEQDEN 

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase."
Koesling D., Herz J., Gausepohl H., Niroomand F., Hinsch K.-D., Muelsch A., Boehme E., Schultz G., Frank R.
FEBS Lett. 239:29-34(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Lung.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Brain cortex.
[3]"Mutation of His-105 in the beta 1 subunit yields a nitric oxide-insensitive form of soluble guanylyl cyclase."
Wedel B., Humbert P., Harteneck C., Foerster J., Malkewitz J., Bohme E., Schultz G., Koesling D.
Proc. Natl. Acad. Sci. U.S.A. 91:2592-2596(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF HISTIDINE RESIDUES.
[4]"Identification of histidine 105 in the beta1 subunit of soluble guanylate cyclase as the heme proximal ligand."
Zhao Y., Schelvis J.P., Babcock G.T., Marletta M.A.
Biochemistry 37:4502-4509(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: HEME PROXIMAL LIGAND.
[5]"Catalytic mechanism of the adenylyl and guanylyl cyclases: modeling and mutational analysis."
Liu Y., Ruoho A.E., Rao V.D., Hurley J.H.
Proc. Natl. Acad. Sci. U.S.A. 94:13414-13419(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 412-572.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00770 mRNA. Translation: CAA68739.1.
BC133308 mRNA. Translation: AAI33309.1.
PIROYBO70. S01653.
RefSeqNP_777066.1. NM_174641.1.
UniGeneBt.109786.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AWNmodel-B412-572[»]
ProteinModelPortalP16068.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP16068.

Proteomic databases

PRIDEP16068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000005009; ENSBTAP00000005009; ENSBTAG00000003840.
GeneID282433.
KEGGbta:282433.

Organism-specific databases

CTD2983.

Phylogenomic databases

eggNOGCOG2114.
GeneTreeENSGT00750000117642.
HOGENOMHOG000220903.
HOVERGENHBG051715.
InParanoidP16068.
KOK12319.
OMAVLMSEQF.

Family and domain databases

Gene3D3.30.70.1230. 1 hit.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011645. Haem_no_assoc-bd.
IPR011644. Heme_NO-bd.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
[Graphical view]
PfamPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806209.

Entry information

Entry nameGCYB1_BOVIN
AccessionPrimary (citable) accession number: P16068
Secondary accession number(s): A2VDM4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 14, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references