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Protein

Atrial natriuretic peptide receptor 2

Gene

Npr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.By similarity

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.By similarity

GO - Molecular functioni

  • ATP binding Source: RGD
  • GTP binding Source: RGD
  • guanylate cyclase activity Source: RGD
  • natriuretic peptide receptor activity Source: RGD
  • peptide hormone binding Source: RGD
  • protein kinase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis, Osteogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2. 5301.
ReactomeiR-RNO-5578768. Physiological factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 2 (EC:4.6.1.2By similarity)
Alternative name(s):
Atrial natriuretic peptide receptor type B
Short name:
ANP-B
Short name:
ANPR-B
Short name:
NPR-B
Guanylate cyclase B
Short name:
GC-B
Gene namesi
Name:Npr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620851. Npr2.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 458ExtracellularSequence analysisAdd BLAST442
Transmembranei459 – 478HelicalSequence analysisAdd BLAST20
Topological domaini479 – 1047CytoplasmicSequence analysisAdd BLAST569

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi513S → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi516T → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi518S → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi521G → A: Reduced phosphorylation, 50% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi522S → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi523S → A: Markedly reduced phosphorylation, 70% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi526S → A: Markedly reduced phosphorylation, 80% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi529T → A: No effect on phosphorylation, 30% loss of CNP-dependent activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001236617 – 1047Atrial natriuretic peptide receptor 2Add BLAST1031

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi75 ↔ 101By similarity
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Glycosylationi244N-linked (GlcNAc...)Sequence analysis1
Glycosylationi277N-linked (GlcNAc...)Sequence analysis1
Glycosylationi349N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi439InterchainCurated
Disulfide bondi448InterchainCurated
Modified residuei513Phosphoserine2 Publications1
Modified residuei516Phosphothreonine2 Publications1
Modified residuei518Phosphoserine2 Publications1
Modified residuei522PhosphoserineBy similarity1
Modified residuei523Phosphoserine2 Publications1
Modified residuei526Phosphoserine2 Publications1
Modified residuei529Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation of the protein kinase-like domain is required for full activation by CNP.2 Publications
Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP16067.
PRIDEiP16067.

PTM databases

iPTMnetiP16067.
PhosphoSitePlusiP16067.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015991.
GenevisibleiP16067. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021802.

Structurei

3D structure databases

ProteinModelPortaliP16067.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini513 – 786Protein kinasePROSITE-ProRule annotationAdd BLAST274
Domaini861 – 991Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP16067.
KOiK12324.
OMAiHQVYTRE.
OrthoDBiEOG091G02QS.
PhylomeDBiP16067.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPSLLLVV AALAGGVRPP GARNLTLAVV LPEHNLSYAW AWPRVGPAVA
60 70 80 90 100
LAVEALGRAL PVDLRFVSSE LDGACSEYLA PLRAVDLKLY HDPDLLLGPG
110 120 130 140 150
CVYPAASVAR FASHWHLPLL TAGAVASGFA AKNEHYRTLV RTGPSAPKLG
160 170 180 190 200
EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH
210 220 230 240 250
QVYTREPGGP EQATHFIRAN GRIVYICGPL EMLHEILLQA QRENLTNGDY
260 270 280 290 300
VFFYLDVFGE SLRAGPTRAT GRPWQDNRTQ EQAQALREAF QTVLVITYRE
310 320 330 340 350
PPNPEYQEFQ NRLLIRARED FGVELAPSLM NLIAGCFYDG ILLYAQVLNE
360 370 380 390 400
TIQEGGTRED GLRIVEKMQG RRYHGVTGLV VMDKNNDRET DFVLWAMGDL
410 420 430 440 450
ESGDFQPAAH YSGAEKQIWW TGRPIPWVKG APPLDNPPCA FDLDDPSCDK
460 470 480 490 500
TPLSTLAIVA LGTGITFIMF GVSSFLIFRK LMLEKELASM LWRIRWEELQ
510 520 530 540 550
FGNSDRYHKG AGSRLTLSLR GSSYGSLMTA HGKYQIFANT GHFKGNVVAI
560 570 580 590 600
KHVNKKRIEL TRQVLFELKH MRDVQFNHLT RFIGACIDPP NICIVTEYCP
610 620 630 640 650
RGSLQDILEN DSINLDWMFR YSLINDLVKG MAFLHNSIIS SHGSLKSSNC
660 670 680 690 700
VVDSRFVLKI TDYGLASFRS TAEPDDSHAL YAKKLWTAPE LLSGNPLPTT
710 720 730 740 750
GMQKADVYSF AIILQEIALR SGPFYLEGLD LSPKEIVQKV RNGQRPYFRP
760 770 780 790 800
SIDRTQLNEE LVLLMERCWA QDPTERPDFG QIKGFIRRFN KEGGTSILDN
810 820 830 840 850
LLLRMEQYAN NLEKLVEERT QAYLEEKRKA EALLYQILPH SVAEQLKRGE
860 870 880 890 900
TVQAEAFDSV TIYFSDIVGF TALSAESTPM QVVTLLNDLY TCFDAIIDNF
910 920 930 940 950
DVYKVETIGD AYMVVSGLPG RNGQRHAPEI ARMALALLDA VSSFRIRHRP
960 970 980 990 1000
HDQLRLRIGV HTGPVCAGVV GLKMPRYCLF GDTVNTASRM ESNGQALKIH
1010 1020 1030 1040
VSSTTKDALD ELGCFQLELR GDVEMKGKGK MRTYWLLGER KGPPGLL
Length:1,047
Mass (Da):117,127
Last modified:April 1, 1990 - v1
Checksum:i5062C49228CC14A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26896 mRNA. Translation: AAA41205.1.
PIRiA33300. OYRTBR.
RefSeqiNP_446290.1. NM_053838.1.
UniGeneiRn.32984.

Genome annotation databases

EnsembliENSRNOT00000021802; ENSRNOP00000021802; ENSRNOG00000015991.
GeneIDi116564.
KEGGirno:116564.
UCSCiRGD:620851. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26896 mRNA. Translation: AAA41205.1.
PIRiA33300. OYRTBR.
RefSeqiNP_446290.1. NM_053838.1.
UniGeneiRn.32984.

3D structure databases

ProteinModelPortaliP16067.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021802.

PTM databases

iPTMnetiP16067.
PhosphoSitePlusiP16067.

Proteomic databases

PaxDbiP16067.
PRIDEiP16067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021802; ENSRNOP00000021802; ENSRNOG00000015991.
GeneIDi116564.
KEGGirno:116564.
UCSCiRGD:620851. rat.

Organism-specific databases

CTDi4882.
RGDi620851. Npr2.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP16067.
KOiK12324.
OMAiHQVYTRE.
OrthoDBiEOG091G02QS.
PhylomeDBiP16067.
TreeFamiTF106338.

Enzyme and pathway databases

BRENDAi4.6.1.2. 5301.
ReactomeiR-RNO-5578768. Physiological factors.

Miscellaneous databases

PROiP16067.

Gene expression databases

BgeeiENSRNOG00000015991.
GenevisibleiP16067. RN.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANPRB_RAT
AccessioniPrimary (citable) accession number: P16067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.