SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P16067

- ANPRB_RAT

UniProt

P16067 - ANPRB_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Atrial natriuretic peptide receptor 2
Gene
Npr2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth By similarity.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. GTP binding Source: RGD
  3. guanylate cyclase activity Source: RGD
  4. natriuretic peptide receptor activity Source: RGD
  5. peptide hormone binding Source: RGD
  6. protein kinase activity Source: InterPro

GO - Biological processi

  1. bone development Source: Ensembl
  2. cGMP biosynthetic process Source: RGD
  3. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: Ensembl
  4. intracellular signal transduction Source: InterPro
  5. ossification Source: UniProtKB-KW
  6. receptor guanylyl cyclase signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis, Osteogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 2 (EC:4.6.1.2)
Alternative name(s):
Atrial natriuretic peptide receptor type B
Short name:
ANP-B
Short name:
ANPR-B
Short name:
NPR-B
Guanylate cyclase B
Short name:
GC-B
Gene namesi
Name:Npr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi620851. Npr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 458442Extracellular Reviewed prediction
Add
BLAST
Transmembranei459 – 47820Helical; Reviewed prediction
Add
BLAST
Topological domaini479 – 1047569Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi513 – 5131S → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication
Mutagenesisi516 – 5161T → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication
Mutagenesisi518 – 5181S → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication
Mutagenesisi521 – 5211G → A: Reduced phosphorylation, 50% loss of CNP-dependent activity. 1 Publication
Mutagenesisi522 – 5221S → A: Reduced phosphorylation, 30% loss of CNP-dependent activity. 1 Publication
Mutagenesisi523 – 5231S → A: Markedly reduced phosphorylation, 70% loss of CNP-dependent activity. 1 Publication
Mutagenesisi526 – 5261S → A: Markedly reduced phosphorylation, 80% loss of CNP-dependent activity. 1 Publication
Mutagenesisi529 – 5291T → A: No effect on phosphorylation, 30% loss of CNP-dependent activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed prediction
Add
BLAST
Chaini17 – 10471031Atrial natriuretic peptide receptor 2
PRO_0000012366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi75 ↔ 101 By similarity
Glycosylationi161 – 1611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi195 – 1951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi244 – 2441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi277 – 2771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi349 – 3491N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi439 – 439Interchain Inferred
Disulfide bondi448 – 448Interchain Inferred
Modified residuei513 – 5131Phosphoserine2 Publications
Modified residuei516 – 5161Phosphothreonine2 Publications
Modified residuei518 – 5181Phosphoserine2 Publications
Modified residuei523 – 5231Phosphoserine2 Publications
Modified residuei526 – 5261Phosphoserine2 Publications
Modified residuei529 – 5291Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation of the protein kinase-like domain is required for full activation by CNP.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP16067.
PRIDEiP16067.

PTM databases

PhosphoSiteiP16067.

Expressioni

Gene expression databases

GenevestigatoriP16067.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021802.

Structurei

3D structure databases

ProteinModelPortaliP16067.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini513 – 786274Protein kinase
Add
BLAST
Domaini861 – 991131Guanylate cyclase
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00710000106571.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP16067.
KOiK12324.
OMAiAAKSEHY.
OrthoDBiEOG7Z69BJ.
PhylomeDBiP16067.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16067-1 [UniParc]FASTAAdd to Basket

« Hide

MALPSLLLVV AALAGGVRPP GARNLTLAVV LPEHNLSYAW AWPRVGPAVA     50
LAVEALGRAL PVDLRFVSSE LDGACSEYLA PLRAVDLKLY HDPDLLLGPG 100
CVYPAASVAR FASHWHLPLL TAGAVASGFA AKNEHYRTLV RTGPSAPKLG 150
EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH 200
QVYTREPGGP EQATHFIRAN GRIVYICGPL EMLHEILLQA QRENLTNGDY 250
VFFYLDVFGE SLRAGPTRAT GRPWQDNRTQ EQAQALREAF QTVLVITYRE 300
PPNPEYQEFQ NRLLIRARED FGVELAPSLM NLIAGCFYDG ILLYAQVLNE 350
TIQEGGTRED GLRIVEKMQG RRYHGVTGLV VMDKNNDRET DFVLWAMGDL 400
ESGDFQPAAH YSGAEKQIWW TGRPIPWVKG APPLDNPPCA FDLDDPSCDK 450
TPLSTLAIVA LGTGITFIMF GVSSFLIFRK LMLEKELASM LWRIRWEELQ 500
FGNSDRYHKG AGSRLTLSLR GSSYGSLMTA HGKYQIFANT GHFKGNVVAI 550
KHVNKKRIEL TRQVLFELKH MRDVQFNHLT RFIGACIDPP NICIVTEYCP 600
RGSLQDILEN DSINLDWMFR YSLINDLVKG MAFLHNSIIS SHGSLKSSNC 650
VVDSRFVLKI TDYGLASFRS TAEPDDSHAL YAKKLWTAPE LLSGNPLPTT 700
GMQKADVYSF AIILQEIALR SGPFYLEGLD LSPKEIVQKV RNGQRPYFRP 750
SIDRTQLNEE LVLLMERCWA QDPTERPDFG QIKGFIRRFN KEGGTSILDN 800
LLLRMEQYAN NLEKLVEERT QAYLEEKRKA EALLYQILPH SVAEQLKRGE 850
TVQAEAFDSV TIYFSDIVGF TALSAESTPM QVVTLLNDLY TCFDAIIDNF 900
DVYKVETIGD AYMVVSGLPG RNGQRHAPEI ARMALALLDA VSSFRIRHRP 950
HDQLRLRIGV HTGPVCAGVV GLKMPRYCLF GDTVNTASRM ESNGQALKIH 1000
VSSTTKDALD ELGCFQLELR GDVEMKGKGK MRTYWLLGER KGPPGLL 1047
Length:1,047
Mass (Da):117,127
Last modified:April 1, 1990 - v1
Checksum:i5062C49228CC14A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26896 mRNA. Translation: AAA41205.1.
PIRiA33300. OYRTBR.
RefSeqiNP_446290.1. NM_053838.1.
UniGeneiRn.32984.

Genome annotation databases

EnsembliENSRNOT00000021802; ENSRNOP00000021802; ENSRNOG00000015991.
GeneIDi116564.
KEGGirno:116564.
UCSCiRGD:620851. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26896 mRNA. Translation: AAA41205.1 .
PIRi A33300. OYRTBR.
RefSeqi NP_446290.1. NM_053838.1.
UniGenei Rn.32984.

3D structure databases

ProteinModelPortali P16067.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000021802.

PTM databases

PhosphoSitei P16067.

Proteomic databases

PaxDbi P16067.
PRIDEi P16067.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000021802 ; ENSRNOP00000021802 ; ENSRNOG00000015991 .
GeneIDi 116564.
KEGGi rno:116564.
UCSCi RGD:620851. rat.

Organism-specific databases

CTDi 4882.
RGDi 620851. Npr2.

Phylogenomic databases

eggNOGi COG2114.
GeneTreei ENSGT00710000106571.
HOGENOMi HOG000293307.
HOVERGENi HBG051862.
InParanoidi P16067.
KOi K12324.
OMAi AAKSEHY.
OrthoDBi EOG7Z69BJ.
PhylomeDBi P16067.
TreeFami TF106338.

Miscellaneous databases

NextBioi 619241.
PROi P16067.

Gene expression databases

Genevestigatori P16067.

Family and domain databases

Gene3Di 3.30.70.1230. 1 hit.
InterProi IPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PRINTSi PR00255. NATPEPTIDER.
SMARTi SM00044. CYCc. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The primary structure of a plasma membrane guanylate cyclase demonstrates diversity within this new receptor family."
    Schulz S., Singh S., Bellet R.A., Singh G., Tubb D.J., Chin H., Garbers D.L.
    Cell 58:1155-1162(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification and characterization of the major phosphorylation sites of the B-type natriuretic peptide receptor."
    Potter L.R., Hunter T.
    J. Biol. Chem. 273:15533-15539(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-513; THR-516; SER-518; SER-523 AND SER-526, MUTAGENESIS OF SER-513; THR-516; SER-518; GLY-521; SER-522; SER-523; SER-526 AND THR-529.
  3. "Mass spectrometric identification of phosphorylation sites in guanylyl cyclase A and B."
    Yoder A.R., Stone M.D., Griffin T.J., Potter L.R.
    Biochemistry 49:10137-10145(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-513; THR-516; SER-518; SER-523; SER-526 AND THR-529.

Entry informationi

Entry nameiANPRB_RAT
AccessioniPrimary (citable) accession number: P16067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi