Reviewed, UniProtKB/Swiss-Prot P16061 (CHI8_POPTR)

Names and origin

Protein names

Recommended name:
    Endochitinase WIN8
    EC=3.2.1.14

Gene names

Name:

WIN8

Organism

Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)

Taxonomic identifier

3694 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 316	293	Endochitinase WIN8		PRO_0000005319
Regions
	Domain	24 – 64	41	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	26 ↔ 41		By similarity
	Disulfide bond	35 ↔ 47		By similarity
	Disulfide bond	40 ↔ 54		By similarity
	Disulfide bond	58 ↔ 62		By similarity
	Disulfide bond	84 ↔ 146		By similarity
	Disulfide bond	158 ↔ 168		By similarity
	Disulfide bond	266 ↔ 298		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16061-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: A441FD604B2C1615	FASTA	316	35,046
	10 20 30 40 50 60 MRFWALTVLS LLLSLLLGVS SDTAQCGSQA GNATCPNDLC CSSGGYCGLT VAYCCAGCVS 70 80 90 100 110 120 QCRNCFFTES MFEQMLPNRN NDSCPGKGFY TYDAYFVATE FYPGFGMTGD DDTRKRELAA 130 140 150 160 170 180 FFAQTSQETS GRSIIGEDAP FTWGYCLVNE LNPNSDYCDP KTKSSYPCVA DYYGRGPLQL 190 200 210 220 230 240 RWNYNYGECG NYLGQNLLDE PEKVATDPVL SFEAALWFWM NPHSTGAPSC HEVITGEWSP 250 260 270 280 290 300 SEADIEAGRK PGFGMLTNII TNGGECTKDG KTRQQNRIDY YLRYCDMLQV DPGDNLYCDN 310 QETFEDNGLL KMVGTM

References

[1]	"Systemic accumulation of specific mRNAs in response to wounding in poplar trees." Parsons T.J., Bradshaw H.D. Jr., Gordon M.P. Proc. Natl. Acad. Sci. U.S.A. 86:7895-7899(1989) [PubMed: 2813366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M25337 mRNA. Translation: AAA96702.1.
PIR	A33985.
3D structure databases
HSSP	HSSP built from PDB template 1HEV based on UniProtKB P02877.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 262297.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI8_POPTR

Accession

Primary (citable) accession number: P16061

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	September 22, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)