Skip Header

Contribute Send feedback
Read comments (?) or add your own

P16053 (NFM_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurofilament medium polypeptide
Short name=NF-M
Alternative name(s):
160 kDa neurofilament protein
Neurofilament triplet M protein
Gene names
Name:NEFM
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Post-translational modification

There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.

Sequence similarities

Belongs to the intermediate filament family.

Ontologies

Keywords
   Cellular componentIntermediate filament
   DomainCoiled coil
   PTMAcetylation
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintermediate filament

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionstructural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 858857Neurofilament medium polypeptide
PRO_0000063799

Regions

Region2 – 9998Head
Region100 – 407308Rod
Region100 – 13132Coil 1A
Region132 – 14413Linker 1
Region145 – 24399Coil 1B
Region244 – 26017Linker 12
Region261 – 28222Coil 2A
Region283 – 2864Linker 2
Region287 – 407121Coil 2B
Region408 – 858451Tail

Amino acid modifications

Modified residue21N-acetylserine By similarity
Glycosylation471O-linked (GlcNAc) By similarity
Glycosylation4271O-linked (GlcNAc) By similarity

Experimental info

Sequence conflict5471G → R in CAA29073. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P16053 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 50D10457BCE2B839

FASTA85895,835
        10         20         30         40         50         60 
MSYTMEPLGN PSYRRVMTET RATYSRASAS PSSGFRSQSW SRGSGSTVSS SYKRTNLGAP 

        70         80         90        100        110        120 
RTAYGSTVLS SAESLDVSQS SLLNGAAELK LSRSNEKEQL QGLNDRFAGY IEKVHYLEQQ 

       130        140        150        160        170        180 
NKEIEAELAA LRQKHAGRAQ LGDAYEQELR ELRGALEQVS HEKAQIQLDS EHIEEDIQRL 

       190        200        210        220        230        240 
RERFEDEARL RDETEATIAA LRKEMEEASL MRAELDKKVQ SLQDEVAFLR GNHEEEVAEL 

       250        260        270        280        290        300 
LAQLQASHAT VERKDYLKTD LTTALKEIRA QLECQSDHNM HQAEEWFKCR YAKLTEAAEQ 

       310        320        330        340        350        360 
NKEAIRSAKE EIAEYRRQLQ SKSIELESVR GTKESLERQL SDIEERHNND LTTYQDTIHQ 

       370        380        390        400        410        420 
LENELRGTKW EMARHLREYQ DLLNVKMALD IEIAAYRKLL EGEETRFSAF SGSITGPIFT 

       430        440        450        460        470        480 
HRQPSVTIAS TKIQKTKIEP PKLKVQHKFV EEIIEETKVE DEKSEMEDAL SAIAEEMAAK 

       490        500        510        520        530        540 
AQEEEQEEEK AEEEAVEEEA VSEKAAEQAA EEEEKEEEEA EEEEAAKSDA AEEGGSKKEE 

       550        560        570        580        590        600 
IEEKEEGEEA EEEEAEAKGK AEEAGAKVEK VKSPPAKSPP KSPPKSPVTE QAKAVQKAAA 

       610        620        630        640        650        660 
EVGKDQKAEK AAEKAAKEEK AASPEKPATP KVTSPEKPAT PEKPPTPEKA ITPEKVRSPE 

       670        680        690        700        710        720 
KPTTPEKVVS PEKPASPEKP RTPEKPASPE KPATPEKPRT PEKPATPEKP RSPEKPSSPL 

       730        740        750        760        770        780 
KDEKAVVEES ITVTKVTKVT AEVEVSKEAR KEDIAVNGEV EEKKDEAKEK EAEEEEKGVV 

       790        800        810        820        830        840 
TNGLDVSPVD EKGEKVVVTK KAEKITSEGG DSTTTYITKS VTVTQKVEEH EESFEEKLVS 

       850 
TKKVEKVTSH AVVKEIKE

« Hide

References

[1]"Isolation of the chicken middle-molecular weight neurofilament (NF-M) gene and characterization of its promoter."
Zopf D., Dineva B., Betz H., Gundelfinger E.D.
Nucleic Acids Res. 18:521-529(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of gene products expressed in the developing chick visual system: characterization of a middle-molecular-weight neurofilament cDNA."
Zopf D., Hermans-Borgmeyer I., Gundelfinger E.D., Betz H.
Genes Dev. 1:699-708(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 260-858.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X17102 Genomic DNA. Translation: CAA34958.1.
X05558 mRNA. Translation: CAA29073.1.
IPIIPI00589424.
PIRS15762.
RefSeqNP_001095200.1. NM_001101730.1.
UniGeneGga.4179.

3D structure databases

ProteinModelPortalP16053.
SMRP16053. Positions 94-131, 325-402.
ModBaseSearch...

Protein-protein interaction databases

IntActP16053. 1 interaction.
STRING9031.ENSGALP00000000422.

Proteomic databases

PaxDbP16053.
PRIDEP16053.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396206.
KEGGgga:396206.

Organism-specific databases

CTD4741.

Phylogenomic databases

eggNOGNOG151229.
HOGENOMHOG000230977.
HOVERGENHBG013015.
InParanoidP16053.
KOK04573.
OrthoDBEOG4VMFFD.

Family and domain databases

InterProIPR016044. F.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERPTHR23239. PTHR23239. 1 hit.
PfamPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
PRINTSPR01248. TYPE1KERATIN.
PROSITEPS00226. IF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816258.

Entry information

Entry nameNFM_CHICK
AccessionPrimary (citable) accession number: P16053
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents