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Protein

Trypsin-1

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei59Charge relay systemBy similarity1
Active sitei103Charge relay systemBy similarity1
Sitei189Required for specificityBy similarity1
Active sitei195Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Trypsin I
OrganismiGadus morhua (Atlantic cod)
Taxonomic identifieri8049 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 13Sequence analysisAdd BLAST13
PropeptideiPRO_000002830914 – 19Activation peptide1 Publication6
ChainiPRO_000002831020 – 241Trypsin-1Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 155PROSITE-ProRule annotation
Disulfide bondi44 ↔ 60PROSITE-ProRule annotation
Disulfide bondi128 ↔ 228PROSITE-ProRule annotation
Disulfide bondi135 ↔ 201PROSITE-ProRule annotation
Disulfide bondi166 ↔ 180PROSITE-ProRule annotation
Disulfide bondi191 ↔ 215PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP16049.

Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni28 – 33Combined sources6
Beta strandi34 – 48Combined sources15
Beta strandi50 – 56Combined sources7
Helixi58 – 60Combined sources3
Beta strandi66 – 70Combined sources5
Beta strandi82 – 91Combined sources10
Turni97 – 100Combined sources4
Beta strandi105 – 111Combined sources7
Beta strandi116 – 118Combined sources3
Beta strandi134 – 141Combined sources8
Beta strandi146 – 148Combined sources3
Beta strandi154 – 160Combined sources7
Helixi163 – 169Combined sources7
Turni171 – 173Combined sources3
Beta strandi178 – 182Combined sources5
Beta strandi198 – 201Combined sources4
Beta strandi204 – 211Combined sources8
Beta strandi213 – 216Combined sources4
Beta strandi222 – 226Combined sources5
Helixi227 – 238Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EEKX-ray1.85A20-239[»]
ProteinModelPortaliP16049.
SMRiP16049.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16049.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 239Peptidase S1PROSITE-ProRule annotationAdd BLAST220

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLIFVLLL GAVFAEEDKI VGGYECTKHS QAHQVSLNSG YHFCGGSLVS
60 70 80 90 100
KDWVVSAAHC YKSVLRVRLG EHHIRVNEGT EQYISSSSVI RHPNYSSYNI
110 120 130 140 150
NNDIMLIKLT KPATLNQYVH AVALPTECAA DATMCTVSGW GNTMSSVADG
160 170 180 190 200
DKLQCLSLPI LSHADCANSY PGMITQSMFC AGYLEGGKDS CQGDSGGPVV
210 220 230 240
CNGVLQGVVS WGYGCAERDH PGVYAKVCVL SGWVRDTMAN Y
Length:241
Mass (Da):25,941
Last modified:November 1, 1997 - v2
Checksum:i44EC9A0106AD1A68
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25E → Q AA sequence (PubMed:2707266).Curated1
Sequence conflicti27 – 28TK → EA AA sequence (PubMed:2707266).Curated2
Sequence conflicti43F → Y AA sequence (PubMed:2707266).Curated1
Sequence conflicti49 – 52VSKD → IN AA sequence (PubMed:2707266).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76886 mRNA. Translation: CAA54214.1.
PIRiS39047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76886 mRNA. Translation: CAA54214.1.
PIRiS39047.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EEKX-ray1.85A20-239[»]
ProteinModelPortaliP16049.
SMRiP16049.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP16049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Miscellaneous databases

EvolutionaryTraceiP16049.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY1_GADMO
AccessioniPrimary (citable) accession number: P16049
Secondary accession number(s): Q91040, Q92156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.