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P16049

- TRY1_GADMO

UniProt

P16049 - TRY1_GADMO

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Protein

Trypsin-1

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei59 – 591Charge relay systemBy similarity
Active sitei103 – 1031Charge relay systemBy similarity
Sitei189 – 1891Required for specificityBy similarity
Active sitei195 – 1951Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Trypsin I
OrganismiGadus morhua (Atlantic cod)
Taxonomic identifieri8049 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313Sequence AnalysisAdd
BLAST
Propeptidei14 – 196Activation peptide1 PublicationPRO_0000028309
Chaini20 – 241222Trypsin-1PRO_0000028310Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 155PROSITE-ProRule annotation
Disulfide bondi44 ↔ 60PROSITE-ProRule annotation
Disulfide bondi128 ↔ 228PROSITE-ProRule annotation
Disulfide bondi135 ↔ 201PROSITE-ProRule annotation
Disulfide bondi166 ↔ 180PROSITE-ProRule annotation
Disulfide bondi191 ↔ 215PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

Secondary structure

1
241
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni28 – 336Combined sources
Beta strandi34 – 4815Combined sources
Beta strandi50 – 567Combined sources
Helixi58 – 603Combined sources
Beta strandi66 – 705Combined sources
Beta strandi82 – 9110Combined sources
Turni97 – 1004Combined sources
Beta strandi105 – 1117Combined sources
Beta strandi116 – 1183Combined sources
Beta strandi134 – 1418Combined sources
Beta strandi146 – 1483Combined sources
Beta strandi154 – 1607Combined sources
Helixi163 – 1697Combined sources
Turni171 – 1733Combined sources
Beta strandi178 – 1825Combined sources
Beta strandi198 – 2014Combined sources
Beta strandi204 – 2118Combined sources
Beta strandi213 – 2164Combined sources
Beta strandi222 – 2265Combined sources
Helixi227 – 23812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EEKX-ray1.85A20-239[»]
ProteinModelPortaliP16049.
SMRiP16049. Positions 20-239.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16049.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 239220Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16049-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSLIFVLLL GAVFAEEDKI VGGYECTKHS QAHQVSLNSG YHFCGGSLVS
60 70 80 90 100
KDWVVSAAHC YKSVLRVRLG EHHIRVNEGT EQYISSSSVI RHPNYSSYNI
110 120 130 140 150
NNDIMLIKLT KPATLNQYVH AVALPTECAA DATMCTVSGW GNTMSSVADG
160 170 180 190 200
DKLQCLSLPI LSHADCANSY PGMITQSMFC AGYLEGGKDS CQGDSGGPVV
210 220 230 240
CNGVLQGVVS WGYGCAERDH PGVYAKVCVL SGWVRDTMAN Y
Length:241
Mass (Da):25,941
Last modified:November 1, 1997 - v2
Checksum:i44EC9A0106AD1A68
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251E → Q AA sequence (PubMed:2707266)Curated
Sequence conflicti27 – 282TK → EA AA sequence (PubMed:2707266)Curated
Sequence conflicti43 – 431F → Y AA sequence (PubMed:2707266)Curated
Sequence conflicti49 – 524VSKD → IN AA sequence (PubMed:2707266)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76886 mRNA. Translation: CAA54214.1.
PIRiS39047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76886 mRNA. Translation: CAA54214.1 .
PIRi S39047.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2EEK X-ray 1.85 A 20-239 [» ]
ProteinModelPortali P16049.
SMRi P16049. Positions 20-239.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG013304.

Miscellaneous databases

EvolutionaryTracei P16049.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen."
    Gudmundsdottir A., Gudmundsdottir E., Oskarsson S., Bjarnason J.B., Eakin A.E., Craik C.S.
    Eur. J. Biochem. 217:1091-1097(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pyloric caecum.
  2. "Purification and characterization of trypsin from the poikilotherm Gadus morhua."
    Asgeirsson B., Fox J.W., Bjarnason J.B.
    Eur. J. Biochem. 180:85-94(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-58.
    Tissue: Pyloric caecum.

Entry informationi

Entry nameiTRY1_GADMO
AccessioniPrimary (citable) accession number: P16049
Secondary accession number(s): Q91040, Q92156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3