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P16049

- TRY1_GADMO

UniProt

P16049 - TRY1_GADMO

Protein

Trypsin-1

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei59 – 591Charge relay systemBy similarity
    Active sitei103 – 1031Charge relay systemBy similarity
    Sitei189 – 1891Required for specificityBy similarity
    Active sitei195 – 1951Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-1 (EC:3.4.21.4)
    Alternative name(s):
    Trypsin I
    OrganismiGadus morhua (Atlantic cod)
    Taxonomic identifieri8049 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1313Sequence AnalysisAdd
    BLAST
    Propeptidei14 – 196Activation peptide1 PublicationPRO_0000028309
    Chaini20 – 241222Trypsin-1PRO_0000028310Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 155PROSITE-ProRule annotation
    Disulfide bondi44 ↔ 60PROSITE-ProRule annotation
    Disulfide bondi128 ↔ 228PROSITE-ProRule annotation
    Disulfide bondi135 ↔ 201PROSITE-ProRule annotation
    Disulfide bondi166 ↔ 180PROSITE-ProRule annotation
    Disulfide bondi191 ↔ 215PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Structurei

    Secondary structure

    1
    241
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni28 – 336
    Beta strandi34 – 4815
    Beta strandi50 – 567
    Helixi58 – 603
    Beta strandi66 – 705
    Beta strandi82 – 9110
    Turni97 – 1004
    Beta strandi105 – 1117
    Beta strandi116 – 1183
    Beta strandi134 – 1418
    Beta strandi146 – 1483
    Beta strandi154 – 1607
    Helixi163 – 1697
    Turni171 – 1733
    Beta strandi178 – 1825
    Beta strandi198 – 2014
    Beta strandi204 – 2118
    Beta strandi213 – 2164
    Beta strandi222 – 2265
    Helixi227 – 23812

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EEKX-ray1.85A20-239[»]
    ProteinModelPortaliP16049.
    SMRiP16049. Positions 20-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16049.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 239220Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG013304.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16049-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSLIFVLLL GAVFAEEDKI VGGYECTKHS QAHQVSLNSG YHFCGGSLVS    50
    KDWVVSAAHC YKSVLRVRLG EHHIRVNEGT EQYISSSSVI RHPNYSSYNI 100
    NNDIMLIKLT KPATLNQYVH AVALPTECAA DATMCTVSGW GNTMSSVADG 150
    DKLQCLSLPI LSHADCANSY PGMITQSMFC AGYLEGGKDS CQGDSGGPVV 200
    CNGVLQGVVS WGYGCAERDH PGVYAKVCVL SGWVRDTMAN Y 241
    Length:241
    Mass (Da):25,941
    Last modified:November 1, 1997 - v2
    Checksum:i44EC9A0106AD1A68
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251E → Q AA sequence (PubMed:2707266)Curated
    Sequence conflicti27 – 282TK → EA AA sequence (PubMed:2707266)Curated
    Sequence conflicti43 – 431F → Y AA sequence (PubMed:2707266)Curated
    Sequence conflicti49 – 524VSKD → IN AA sequence (PubMed:2707266)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76886 mRNA. Translation: CAA54214.1.
    PIRiS39047.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76886 mRNA. Translation: CAA54214.1 .
    PIRi S39047.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EEK X-ray 1.85 A 20-239 [» ]
    ProteinModelPortali P16049.
    SMRi P16049. Positions 20-239.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG013304.

    Miscellaneous databases

    EvolutionaryTracei P16049.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen."
      Gudmundsdottir A., Gudmundsdottir E., Oskarsson S., Bjarnason J.B., Eakin A.E., Craik C.S.
      Eur. J. Biochem. 217:1091-1097(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pyloric caecum.
    2. "Purification and characterization of trypsin from the poikilotherm Gadus morhua."
      Asgeirsson B., Fox J.W., Bjarnason J.B.
      Eur. J. Biochem. 180:85-94(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-58.
      Tissue: Pyloric caecum.

    Entry informationi

    Entry nameiTRY1_GADMO
    AccessioniPrimary (citable) accession number: P16049
    Secondary accession number(s): Q91040, Q92156
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3