Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Capsid scaffolding protein

Gene

UL80

Organism
Simian cytomegalovirus (strain Colburn)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation
Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation
Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation

Catalytic activityi

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47Charge relay systemUniRule annotation1
Active sitei118Charge relay systemUniRule annotation1
Active sitei142Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • nuclear capsid assembly Source: UniProtKB
  • viral release from host cell Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processViral capsid assembly, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid scaffolding proteinUniRule annotation
Alternative name(s):
Capsid protein P40
Protease precursorUniRule annotation
Short name:
pPRUniRule annotation
Cleaved into the following 2 chains:
AssemblinUniRule annotation (EC:3.4.21.97UniRule annotation)
Alternative name(s):
ProteaseUniRule annotation
Assembly proteinUniRule annotation
Alternative name(s):
Capsid assembly proteinUniRule annotation
Gene namesi
Name:UL80
Synonyms:APNG
OrganismiSimian cytomegalovirus (strain Colburn)
Taxonomic identifieri50292 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiMacaca (macaques) [TaxID: 9539]

Subcellular locationi

Capsid scaffolding protein :
  • Host cytoplasm UniRule annotation
Assemblin :
  • Host nucleus UniRule annotation
Assembly protein :
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000272891 – 589Capsid scaffolding proteinAdd BLAST589
ChainiPRO_00000272901 – 249AssemblinUniRule annotationAdd BLAST249
ChainiPRO_0000027292250 – 589Assembly proteinUniRule annotationAdd BLAST340

Post-translational modificationi

Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei249 – 250Cleavage; by assemblin; Release siteUniRule annotation2
Sitei556 – 557Cleavage; by assemblin; Tail site2

Keywords - PTMi

Phosphoprotein

Miscellaneous databases

PMAP-CutDBiP16046.

Interactioni

Subunit structurei

Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP16046.
SMRiP16046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 326Interaction with pAPUniRule annotationAdd BLAST20
Regioni569 – 589Interaction with major capsid proteinUniRule annotationAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi428 – 433Nuclear localization signal 16
Motifi453 – 459Nuclear localization signal 27

Domaini

Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).UniRule annotation

Sequence similaritiesi

Belongs to the herpesviridae capsid scaffolding protein family.UniRule annotation

Family and domain databases

HAMAPiMF_04008. HSV_SCAF. 1 hit.
InterProiView protein in InterPro
IPR001847. Peptidase_S21.
PfamiView protein in Pfam
PF00716. Peptidase_S21. 1 hit.
PRINTSiPR00236. HSVCAPSIDP40.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform Capsid scaffolding protein (identifier: P16046-1) [UniParc]FASTAAdd to basket
Also known as: pPR

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADPVYVGGF LVRYDEPPGE AELFLPSGVV DRWLRDCRGP LPLNVNHDES
60 70 80 90 100
ATVGYVAGLQ NVRAGLFCLG RVTSPKFLDI VQKASEKSEL VSRGPPSESS
110 120 130 140 150
LRPDGVLEFL SGSYSGLSLS SRRDINAADG AAGDAETACF KHVALCSVGR
160 170 180 190 200
RRGTLAVYGR QPDWVMERFP DLTEADREAL RNQLSGSGEV AAKESAESSA
210 220 230 240 250
AAAVDPFQSD SYGLLGNSVD ALYIQERLPK LRYDKRLVGV TARESYVKAS
260 270 280 290 300
VSPAEQETCD IKVEKERPKE PEQSHVPTES MSHPMSAVAT PAASTVAPSQ
310 320 330 340 350
APLALAHDGV YLPKDAFFSL IGASRPLAEA AGARAAYPAV PPPPAYPVMN
360 370 380 390 400
YEDPSSRHFD YSAWLRRPAY DAVPPLPPPP VMPMPYRRRD PMMEEAERAA
410 420 430 440 450
WERGYAPSAY DHYVNNGSWS RSRSGALKRR RERDASSDEE EDMSFPGEAD
460 470 480 490 500
HGKARKRLKA HHGRDNNNSG SDAKGDRYDD IREALQELKR EMLAVRQIAP
510 520 530 540 550
AALLAPAQLA TPVASPTTTT SHQAEASEPQ ASTAAAAPST ASSHGSKSAE
560 570 580
RGVVNASCRV APPLEAVNPP KDMVDLNRRL FVAALNKME
Length:589
Mass (Da):63,715
Last modified:July 11, 2002 - v3
Checksum:i247CBB9DCC4E5A23
GO
Isoform APNG.7 protein (identifier: P16046-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-165: Missing.

Show »
Length:424
Mass (Da):46,021
Checksum:iC1F027FA60158586
GO
Isoform pAP (identifier: P16046-3) [UniParc]FASTAAdd to basket
Also known as: Assembly protein, APGN.5 protein

The sequence of this isoform differs from the canonical sequence as follows:
     1-280: Missing.

Show »
Length:309
Mass (Da):33,382
Checksum:i7BBA3737AC2F91E5
GO
Isoform APNG.4 protein (identifier: P16046-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-348: Missing.

Show »
Length:241
Mass (Da):26,619
Checksum:iA964DA8576FB1A0E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti501A → R in AAA85777 (PubMed:2536099).Curated1
Sequence conflicti537A → AS (PubMed:2536099).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374261 – 348Missing in isoform APNG.4 protein. CuratedAdd BLAST348
Alternative sequenceiVSP_0374271 – 280Missing in isoform pAP. CuratedAdd BLAST280
Alternative sequenceiVSP_0374281 – 165Missing in isoform APNG.7 protein. CuratedAdd BLAST165

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64627 Genomic DNA. Translation: AAA46065.2.
M24205 mRNA. Translation: AAA85777.1.
PIRiA40414. WMBECB.

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSCAF_SCMVC
AccessioniPrimary (citable) accession number: P16046
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 11, 2002
Last modified: June 7, 2017
This is version 95 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families