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Protein

Phosphate carrier protein, mitochondrial

Gene

Slc25a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transport of phosphate groups from the cytosol to the mitochondrial matrix. Phosphate is cotransported with H+. May play a role regulation of the mitochondrial permeability transition pore (mPTP).1 Publication

GO - Molecular functioni

  • phosphate:proton symporter activity Source: RGD

GO - Biological processi

  • hydrogen ion transmembrane transport Source: GOC
  • phosphate ion transmembrane transport Source: GOC
  • proton transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate carrier protein, mitochondrial
Alternative name(s):
Phosphate transport protein
Short name:
PTP
Solute carrier family 25 member 3
Gene namesi
Name:Slc25a3
Synonyms:Phc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70986. Slc25a3.

Subcellular locationi

  • Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini45 – 5713Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei58 – 8023Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini81 – 11535Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei116 – 13520Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini136 – 15520Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei156 – 17722Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini178 – 21235Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei213 – 23220Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini233 – 25523Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei256 – 27823Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini279 – 30830Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei309 – 32719Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini328 – 35629Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4444Mitochondrion2 PublicationsAdd
BLAST
Chaini45 – 356312Phosphate carrier protein, mitochondrialPRO_0000019258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931N6-acetyllysineBy similarity
Modified residuei190 – 1901PhosphotyrosineBy similarity
Modified residuei203 – 2031N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP16036.
PRIDEiP16036.

PTM databases

iPTMnetiP16036.
PhosphoSiteiP16036.
SwissPalmiP16036.

Interactioni

Subunit structurei

Interacts with PPIF; the interaction is impaired by CsA.1 Publication

Protein-protein interaction databases

IntActiP16036. 1 interaction.
STRINGi10116.ENSRNOP00000011494.

Structurei

3D structure databases

ProteinModelPortaliP16036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati57 – 14185Solcar 1Add
BLAST
Repeati154 – 23885Solcar 2Add
BLAST
Repeati255 – 33379Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0767. Eukaryota.
ENOG410XPST. LUCA.
HOGENOMiHOG000164438.
HOVERGENiHBG024440.
InParanoidiP16036.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSSVAHLAR ANPFNAPHLQ LVHDVSGPRS PPGPPRRSRH LAAAAVEGYS
60 70 80 90 100
CEFGSMKYYA LCGFGGVLSC GLTHTAVVPL DLVKCRMQVD PQKYKGIFNG
110 120 130 140 150
FSITLKEDGV RGLAKGWAPT LIGYSMQGLC KFGFYEVFKA LYSNILGEEN
160 170 180 190 200
TYLWRTSLYL AASASAEFFA DIALAPMEAA KVRIQTQPGY ANTLREAVPK
210 220 230 240 250
MYKEEGLNAF YKGVAPVWMR QIPYTMMKFA CFERTVEALY KFVVPKPRSE
260 270 280 290 300
CTKAEQLVVT FVAGYIAGVF CAIVSHPADS VVSVLNKEKG STASQVLQRL
310 320 330 340 350
GFRGVWKGLF ARIIMIGTLT ALQWFIYDSV KVYFRLPRPP PPEMPESLKK

KLGLTE
Length:356
Mass (Da):39,445
Last modified:April 1, 1990 - v1
Checksum:i64AF5FD9DD671DBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23984 mRNA. Translation: AAA41634.1.
PIRiA34350.
UniGeneiRn.3606.

Genome annotation databases

UCSCiRGD:70986. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23984 mRNA. Translation: AAA41634.1.
PIRiA34350.
UniGeneiRn.3606.

3D structure databases

ProteinModelPortaliP16036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16036. 1 interaction.
STRINGi10116.ENSRNOP00000011494.

PTM databases

iPTMnetiP16036.
PhosphoSiteiP16036.
SwissPalmiP16036.

Proteomic databases

PaxDbiP16036.
PRIDEiP16036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:70986. rat.

Organism-specific databases

RGDi70986. Slc25a3.

Phylogenomic databases

eggNOGiKOG0767. Eukaryota.
ENOG410XPST. LUCA.
HOGENOMiHOG000164438.
HOVERGENiHBG024440.
InParanoidiP16036.

Miscellaneous databases

PROiP16036.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Energy-linked anion transport. Cloning, sequencing, and characterization of a full length cDNA encoding the rat liver mitochondrial proton/phosphate symporter."
    Ferreira G.C., Pratt R.D., Pedersen P.L.
    J. Biol. Chem. 264:15628-15633(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 45-62.
    Tissue: Liver.
  2. "Mitochondrial proton/phosphate transporter. An antibody directed against the COOH terminus and proteolytic cleavage experiments provides new insights about its membrane topology."
    Ferreira G.C., Pratt R.D., Pedersen P.L.
    J. Biol. Chem. 265:21202-21206(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY.
  3. "Mitochondrial phosphate transport. Import of the H+/Pi symporter and role of the presequence."
    Pratt R.D., Ferreira G.C., Pedersen P.L.
    J. Biol. Chem. 266:1276-1280(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSIT PEPTIDE CLEAVAGE SITE.
  4. "The mitochondrial phosphate carrier interacts with cyclophilin D and may play a key role in the permeability transition."
    Leung A.W., Varanyuwatana P., Halestrap A.P.
    J. Biol. Chem. 283:26312-26323(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPIF.

Entry informationi

Entry nameiMPCP_RAT
AccessioniPrimary (citable) accession number: P16036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.