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P16033

- PSBA2_SYNY3

UniProt

P16033 - PSBA2_SYNY3

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Protein

Photosystem Q(B) protein 2

Gene

psbA2

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This is one of the two reaction center proteins of photosystem II.By similarity

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

Note: The PsbA/B heterodimer binds P680, the primary electron donor of PSII. It shares a non-heme iron and each subunit binds additional chlorophylls and pheophytin. PsbA provides most of the ligands for the Mn-cluster of the oxygen-evolving complex.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei161 – 1611Tyrosine radical intermediateBy similarity
Metal bindingi170 – 1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calciumUniRule annotation
Metal bindingi170 – 1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1UniRule annotation
Metal bindingi189 – 1891Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2UniRule annotation
Metal bindingi215 – 2151Iron; shared with heterodimeric partnerUniRule annotation
Metal bindingi272 – 2721Iron; shared with heterodimeric partnerUniRule annotation
Metal bindingi332 – 3321Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2; via tele nitrogenUniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1UniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 3UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4UniRule annotation
Metal bindingi344 – 3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation
Metal bindingi344 – 3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4; via carboxylateUniRule annotation

GO - Molecular functioni

  1. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
  2. iron ion binding Source: UniProtKB-HAMAP
  3. oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  1. photosynthetic electron transport in photosystem II Source: InterPro
  2. response to herbicide Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Herbicide resistance, Photosynthesis, Transport

Keywords - Ligandi

Calcium, Iron, Manganese, Metal-binding

Protein family/group databases

TCDBi3.E.2.2.2. the photosynthetic reaction center (prc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem Q(B) protein 2UniRule annotation (EC:1.10.3.9UniRule annotation)
Alternative name(s):
32 kDa thylakoid membrane protein 2UniRule annotation
Photosystem II protein D1 2UniRule annotation
Gene namesi
Name:psbA2UniRule annotation
Synonyms:psbA-2
Ordered Locus Names:slr1311
AND
Name:psbA3UniRule annotation
Synonyms:psbA-3
Ordered Locus Names:sll1867
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cellular thylakoid membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei36 – 5621HelicalUniRule annotationAdd
BLAST
Transmembranei109 – 12921HelicalUniRule annotationAdd
BLAST
Transmembranei141 – 16424HelicalUniRule annotationAdd
BLAST
Transmembranei192 – 21827HelicalUniRule annotationAdd
BLAST
Transmembranei269 – 28921HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane-derived thylakoid photosystem II Source: UniProtKB
  3. thylakoid membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi221 – 2222SS → LA: Strong herbicide resistance.
Mutagenesisi227 – 2282TT → AA: Herbicide resistance.
Mutagenesisi237 – 2371Y → F: Weak herbicide resistance.
Mutagenesisi238 – 2381K → V: Weak herbicide resistance.
Mutagenesisi255 – 2551F → W: Herbicide resistance.
Mutagenesisi257 – 2571R → V: Herbicide resistance.
Mutagenesisi264 – 2641S → A: Strong herbicide resistance.
Mutagenesisi271 – 2711L → V, M, A or S: Herbicide resistance.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Photosystem Q(B) protein 2PRO_0000090493Add
BLAST
Propeptidei345 – 36016Cleavage; by CtpA1 PublicationUniRule annotationPRO_0000316429Add
BLAST

Post-translational modificationi

C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and photosynthetic growth.1 Publication
Tyr-161 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.By similarity

Proteomic databases

PaxDbiP16033.

Interactioni

Subunit structurei

The PsbA/B heterodimer binds the P680 chlorophylls and subsequent electron acceptors. PSII consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. PSII forms dimeric complexes (By similarity).By similarity

Protein-protein interaction databases

IntActiP16033. 5 interactions.
STRINGi1148.slr1311.

Structurei

3D structure databases

ProteinModelPortaliP16033.
SMRiP16033. Positions 10-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG04871.
HOGENOMiHOG000246913.
InParanoidiP16033.
KOiK02703.
OMAiCFTIAFI.
OrthoDBiEOG6Q2SGP.
PhylomeDBiP16033.

Family and domain databases

Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16033-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTTLQQRES ASLWEQFCQW VTSTNNRIYV GWFGTLMIPT LLTATTCFII
60 70 80 90 100
AFIAAPPVDI DGIREPVAGS LLYGNNIISG AVVPSSNAIG LHFYPIWEAA
110 120 130 140 150
SLDEWLYNGG PYQLVVFHFL IGIFCYMGRQ WELSYRLGMR PWICVAYSAP
160 170 180 190 200
VSAATAVFLI YPIGQGSFSD GMPLGISGTF NFMIVFQAEH NILMHPFHML
210 220 230 240 250
GVAGVFGGSL FSAMHGSLVT SSLVRETTEV ESQNYGYKFG QEEETYNIVA
260 270 280 290 300
AHGYFGRLIF QYASFNNSRS LHFFLGAWPV IGIWFTAMGV STMAFNLNGF
310 320 330 340 350
NFNQSILDSQ GRVIGTWADV LNRANIGFEV MHERNAHNFP LDLASGEQAP
360
VALTAPAVNG
Length:360
Mass (Da):39,721
Last modified:April 1, 1990 - v1
Checksum:i2FE4088498A416C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56000 Genomic DNA. Translation: CAA39472.1.
X13547 Genomic DNA. Translation: CAA31899.1.
BA000022 Genomic DNA. Translation: BAA18230.1.
BA000022 Genomic DNA. Translation: BAA16586.1.
PIRiS13112. F2YB16.
RefSeqiNP_439906.1. NC_000911.1.
NP_441550.1. NC_000911.1.
YP_005649961.1. NC_017277.1.
YP_005651608.1. NC_017277.1.
YP_007449789.1. NC_020286.1.
YP_007451432.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA16586; BAA16586; BAA16586.
BAA18230; BAA18230; BAA18230.
GeneIDi951890.
953105.
KEGGisyn:sll1867.
syn:slr1311.
PATRICi23836870. VBISynSp132158_0010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56000 Genomic DNA. Translation: CAA39472.1 .
X13547 Genomic DNA. Translation: CAA31899.1 .
BA000022 Genomic DNA. Translation: BAA18230.1 .
BA000022 Genomic DNA. Translation: BAA16586.1 .
PIRi S13112. F2YB16.
RefSeqi NP_439906.1. NC_000911.1.
NP_441550.1. NC_000911.1.
YP_005649961.1. NC_017277.1.
YP_005651608.1. NC_017277.1.
YP_007449789.1. NC_020286.1.
YP_007451432.1. NC_020286.1.

3D structure databases

ProteinModelPortali P16033.
SMRi P16033. Positions 10-344.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P16033. 5 interactions.
STRINGi 1148.slr1311.

Protein family/group databases

TCDBi 3.E.2.2.2. the photosynthetic reaction center (prc) family.

Proteomic databases

PaxDbi P16033.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA16586 ; BAA16586 ; BAA16586 .
BAA18230 ; BAA18230 ; BAA18230 .
GeneIDi 951890.
953105.
KEGGi syn:sll1867.
syn:slr1311.
PATRICi 23836870. VBISynSp132158_0010.

Phylogenomic databases

eggNOGi NOG04871.
HOGENOMi HOG000246913.
InParanoidi P16033.
KOi K02703.
OMAi CFTIAFI.
OrthoDBi EOG6Q2SGP.
PhylomeDBi P16033.

Family and domain databases

Gene3Di 1.20.85.10. 1 hit.
HAMAPi MF_01379. PSII_PsbA_D1.
InterProi IPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view ]
Pfami PF00124. Photo_RC. 1 hit.
[Graphical view ]
PRINTSi PR00256. REACTNCENTRE.
SUPFAMi SSF81483. SSF81483. 1 hit.
TIGRFAMsi TIGR01151. psbA. 1 hit.
PROSITEi PS00244. REACTION_CENTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a second psbA gene from the unicellular cyanobacterium Synechocystis 6803."
    Ravnikar P.D., Debus R., Sevrinck J., Saetaert P., McIntosh L.
    Nucleic Acids Res. 17:3991-3991(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the psbA3 gene from the cyanobacterium Synechocystis PCC 6803."
    Metz J., Nixon P., Diner B.
    Nucleic Acids Res. 18:6715-6715(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  4. "Mutations in the D1 subunit of photosystem II distinguish between quinone and herbicide binding sites."
    Ohad N., Hirschberg J.
    Plant Cell 4:273-282(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS FOR HERBICIDE RESISTANCE.
  5. "The D-E region of the D1 protein is involved in multiple quinone and herbicide interactions in photosystem II."
    Kless H., Oren-Shamir M., Malkin S., McIntosh L., Edelman M.
    Biochemistry 33:10501-10507(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS FOR HERBICIDE RESISTANCE.
  6. "Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease. Analysis of a spontaneous photosystem II-deficient mutant strain of the cyanobacterium Synechocystis sp. PCC 6803."
    Shestakov S.V., Anbudurai P.R., Stanbekova G.E., Gadzhiev A., Lind L.K., Pakrasi H.B.
    J. Biol. Chem. 269:19354-19359(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE CLEAVAGE.
    Strain: ATCC 27184 / PCC 6803 / N-1.
  7. "Thylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis."
    Pursiheimo S., Rintamaeki E., Baena-Gonzalez E., Aro E.-M.
    FEBS Lett. 423:178-182(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, LACK OF PHOSPHORYLATION.
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiPSBA2_SYNY3
AccessioniPrimary (citable) accession number: P16033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Herbicides such as atrazine, BNT, diuron or ioxynil bind to Q(B) and block electron transport.
Cyanobacteria usually contain more than 2 copies of the psbA gene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3