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Protein

Photosystem II protein D1 2

Gene

psbA2

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Photosystem II (PSII) is a light-driven water: plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.UniRule annotation

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

Note: The D1/D2 heterodimer binds P680, chlorophylls that are the primary electron donor of PSII, and subsequent electron acceptors. It shares a non-heme iron and each subunit binds pheophytin, quinone, additional chlorophylls, carotenoids and lipids. D1 provides most of the ligands for the Mn4-Ca-O5 cluster of the oxygen-evolving complex (OEC). There is also a Cl(-1) ion associated with D1 and D2, which is required for oxygen evolution. The PSII complex binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi118Magnesium (chlorophyll-a ChlzD1 axial ligand); via tele nitrogenUniRule annotation1
Binding sitei126Pheophytin D1UniRule annotation1
Sitei161Tyrosine radical intermediateUniRule annotation1
Metal bindingi170Calcium-manganese-oxide [Ca-4Mn-5O]; calciumUniRule annotation1
Metal bindingi170Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation1
Metal bindingi189Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation1
Sitei190Stabilizes free radical intermediateUniRule annotation1
Metal bindingi198Magnesium (chlorophyll-a PD1 axial ligand); via tele nitrogenUniRule annotation1
Metal bindingi215Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation1
Binding sitei215Quinone (B)UniRule annotation1
Metal bindingi272Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation1
Metal bindingi332Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi333Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 3UniRule annotation1
Metal bindingi333Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation1
Metal bindingi342Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation1
Metal bindingi342Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2UniRule annotation1
Metal bindingi344Calcium-manganese-oxide [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation1
Metal bindingi344Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2; via carboxylateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Herbicide resistance, Photosynthesis, Transport

Keywords - Ligandi

Calcium, Chlorophyll, Chromophore, Iron, Magnesium, Manganese, Metal-binding

Protein family/group databases

TCDBi3.E.2.2.2. the photosynthetic reaction center (prc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II protein D1 2UniRule annotation (EC:1.10.3.9UniRule annotation)
Short name:
PSII D1 protein 2UniRule annotation
Alternative name(s):
Photosystem II Q(B) protein 2UniRule annotation
Gene namesi
Name:psbA2UniRule annotationCurated
Synonyms:psbA-2
Ordered Locus Names:slr1311
AND
Name:psbA3UniRule annotationCurated
Synonyms:psbA-3
Ordered Locus Names:sll1867
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cellular thylakoid membrane UniRule annotation1 Publication1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 46HelicalUniRule annotationAdd BLAST18
Transmembranei118 – 133HelicalUniRule annotationAdd BLAST16
Transmembranei142 – 156HelicalUniRule annotationAdd BLAST15
Transmembranei197 – 218HelicalUniRule annotationAdd BLAST22
Transmembranei274 – 288HelicalUniRule annotationAdd BLAST15

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi221 – 222SS → LA: Strong herbicide resistance. 1 Publication2
Mutagenesisi227 – 228TT → AA: Herbicide resistance. 1 Publication2
Mutagenesisi237Y → F: Weak herbicide resistance. 1 Publication1
Mutagenesisi238K → V: Weak herbicide resistance. 1 Publication1
Mutagenesisi255F → W: Herbicide resistance. 1 Publication1
Mutagenesisi257R → V: Herbicide resistance. 1 Publication1
Mutagenesisi264S → A: Strong herbicide resistance. 1 Publication1
Mutagenesisi271L → V, M, A or S: Herbicide resistance. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000904931 – 344Photosystem II protein D1 2UniRule annotationAdd BLAST344
PropeptideiPRO_0000316429345 – 360UniRule annotation1 PublicationAdd BLAST16

Post-translational modificationi

C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and photosynthetic growth.UniRule annotation1 Publication
Tyr-161 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.UniRule annotation
C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and thus photosynthetic growth.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei344 – 345Cleavage; by CtpAUniRule annotation1 Publication2

Proteomic databases

PRIDEiP16033.

Interactioni

Subunit structurei

Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP16033. 5 interactors.

Structurei

3D structure databases

ProteinModelPortaliP16033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 265Quinone (B)UniRule annotation2

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000246913.
InParanoidiP16033.
KOiK02703.
OMAiCFTIAFI.
PhylomeDBiP16033.

Family and domain databases

CDDicd09289. Photosystem-II_D1. 1 hit.
Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1. 1 hit.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTLQQRES ASLWEQFCQW VTSTNNRIYV GWFGTLMIPT LLTATTCFII
60 70 80 90 100
AFIAAPPVDI DGIREPVAGS LLYGNNIISG AVVPSSNAIG LHFYPIWEAA
110 120 130 140 150
SLDEWLYNGG PYQLVVFHFL IGIFCYMGRQ WELSYRLGMR PWICVAYSAP
160 170 180 190 200
VSAATAVFLI YPIGQGSFSD GMPLGISGTF NFMIVFQAEH NILMHPFHML
210 220 230 240 250
GVAGVFGGSL FSAMHGSLVT SSLVRETTEV ESQNYGYKFG QEEETYNIVA
260 270 280 290 300
AHGYFGRLIF QYASFNNSRS LHFFLGAWPV IGIWFTAMGV STMAFNLNGF
310 320 330 340 350
NFNQSILDSQ GRVIGTWADV LNRANIGFEV MHERNAHNFP LDLASGEQAP
360
VALTAPAVNG
Length:360
Mass (Da):39,721
Last modified:April 1, 1990 - v1
Checksum:i2FE4088498A416C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56000 Genomic DNA. Translation: CAA39472.1.
X13547 Genomic DNA. Translation: CAA31899.1.
BA000022 Genomic DNA. Translation: BAA18230.1.
BA000022 Genomic DNA. Translation: BAA16586.1.
PIRiS13112. F2YB16.

Genome annotation databases

EnsemblBacteriaiBAA16586; BAA16586; BAA16586.
BAA18230; BAA18230; BAA18230.
KEGGisyn:sll1867.
syn:slr1311.
PATRICi23836870. VBISynSp132158_0010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56000 Genomic DNA. Translation: CAA39472.1.
X13547 Genomic DNA. Translation: CAA31899.1.
BA000022 Genomic DNA. Translation: BAA18230.1.
BA000022 Genomic DNA. Translation: BAA16586.1.
PIRiS13112. F2YB16.

3D structure databases

ProteinModelPortaliP16033.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16033. 5 interactors.

Protein family/group databases

TCDBi3.E.2.2.2. the photosynthetic reaction center (prc) family.

Proteomic databases

PRIDEiP16033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16586; BAA16586; BAA16586.
BAA18230; BAA18230; BAA18230.
KEGGisyn:sll1867.
syn:slr1311.
PATRICi23836870. VBISynSp132158_0010.

Phylogenomic databases

HOGENOMiHOG000246913.
InParanoidiP16033.
KOiK02703.
OMAiCFTIAFI.
PhylomeDBiP16033.

Family and domain databases

CDDicd09289. Photosystem-II_D1. 1 hit.
Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1. 1 hit.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSBA2_SYNY3
AccessioniPrimary (citable) accession number: P16033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Cyanobacteria usually contain more than 2 copies of the psbA gene.UniRule annotation
2 of the reaction center chlorophylls (ChlD1 and ChlD2) are entirely coordinated by water.UniRule annotation
Herbicides such as atrazine, BNT, diuron or ioxynil bind in the Q(B) binding site and block subsequent electron transfer.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.