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P16033

- PSBA2_SYNY3

UniProt

P16033 - PSBA2_SYNY3

Protein

Photosystem Q(B) protein 2

Gene

psbA2

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    This is one of the two reaction center proteins of photosystem II.By similarity

    Catalytic activityi

    2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

    Cofactori

    The PsbA/B heterodimer binds P680, the primary electron donor of PSII. It shares a non-heme iron and each subunit binds additional chlorophylls and pheophytin. PsbA provides most of the ligands for the Mn-cluster of the oxygen-evolving complex.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi170 – 1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calciumUniRule annotation
    Metal bindingi170 – 1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1UniRule annotation
    Metal bindingi189 – 1891Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2UniRule annotation
    Metal bindingi215 – 2151Iron; shared with heterodimeric partnerUniRule annotation
    Metal bindingi272 – 2721Iron; shared with heterodimeric partnerUniRule annotation
    Metal bindingi332 – 3321Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2; via tele nitrogenUniRule annotation
    Metal bindingi333 – 3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1UniRule annotation
    Metal bindingi333 – 3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 3UniRule annotation
    Metal bindingi342 – 3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2UniRule annotation
    Metal bindingi342 – 3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4UniRule annotation
    Sitei344 – 3452Cleavage; by CtpAUniRule annotation
    Metal bindingi344 – 3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation
    Metal bindingi344 – 3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4; via carboxylateUniRule annotation

    GO - Molecular functioni

    1. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
    2. iron ion binding Source: UniProtKB-HAMAP
    3. oxidoreductase activity Source: UniProtKB-KW

    GO - Biological processi

    1. photosynthetic electron transport in photosystem II Source: InterPro
    2. response to herbicide Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Herbicide resistance, Photosynthesis, Transport

    Keywords - Ligandi

    Calcium, Iron, Manganese, Metal-binding

    Protein family/group databases

    TCDBi3.E.2.2.2. the photosynthetic reaction center (prc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Photosystem Q(B) protein 2UniRule annotation (EC:1.10.3.9UniRule annotation)
    Alternative name(s):
    32 kDa thylakoid membrane protein 2UniRule annotation
    Photosystem II protein D1 2UniRule annotation
    Gene namesi
    Name:psbA2UniRule annotation
    Synonyms:psbA-2
    Ordered Locus Names:slr1311
    AND
    Name:psbA3UniRule annotation
    Synonyms:psbA-3
    Ordered Locus Names:sll1867
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    Cellular thylakoid membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane-derived thylakoid photosystem II Source: UniProtKB
    3. thylakoid membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Photosystem II, Thylakoid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi221 – 2222SS → LA: Strong herbicide resistance.
    Mutagenesisi227 – 2282TT → AA: Herbicide resistance.
    Mutagenesisi237 – 2371Y → F: Weak herbicide resistance.
    Mutagenesisi238 – 2381K → V: Weak herbicide resistance.
    Mutagenesisi255 – 2551F → W: Herbicide resistance.
    Mutagenesisi257 – 2571R → V: Herbicide resistance.
    Mutagenesisi264 – 2641S → A: Strong herbicide resistance.
    Mutagenesisi271 – 2711L → V, M, A or S: Herbicide resistance.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Photosystem Q(B) protein 2PRO_0000090493Add
    BLAST
    Propeptidei345 – 36016UniRule annotationPRO_0000316429Add
    BLAST

    Proteomic databases

    PaxDbiP16033.

    Interactioni

    Subunit structurei

    The PsbA/B heterodimer binds the P680 chlorophylls and subsequent electron acceptors. PSII consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. PSII forms dimeric complexes By similarity.By similarity

    Protein-protein interaction databases

    IntActiP16033. 5 interactions.
    STRINGi1148.slr1311.

    Structurei

    3D structure databases

    ProteinModelPortaliP16033.
    SMRiP16033. Positions 10-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621HelicalUniRule annotationAdd
    BLAST
    Transmembranei109 – 12921HelicalUniRule annotationAdd
    BLAST
    Transmembranei141 – 16424HelicalUniRule annotationAdd
    BLAST
    Transmembranei192 – 21827HelicalUniRule annotationAdd
    BLAST
    Transmembranei269 – 28921HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG04871.
    HOGENOMiHOG000246913.
    KOiK02703.
    OMAiCFTIAFI.
    OrthoDBiEOG6Q2SGP.
    PhylomeDBiP16033.

    Family and domain databases

    Gene3Di1.20.85.10. 1 hit.
    HAMAPiMF_01379. PSII_PsbA_D1.
    InterProiIPR000484. Photo_RC_L/M.
    IPR005867. PSII_D1.
    [Graphical view]
    PfamiPF00124. Photo_RC. 1 hit.
    [Graphical view]
    PRINTSiPR00256. REACTNCENTRE.
    SUPFAMiSSF81483. SSF81483. 1 hit.
    TIGRFAMsiTIGR01151. psbA. 1 hit.
    PROSITEiPS00244. REACTION_CENTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16033-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTTLQQRES ASLWEQFCQW VTSTNNRIYV GWFGTLMIPT LLTATTCFII    50
    AFIAAPPVDI DGIREPVAGS LLYGNNIISG AVVPSSNAIG LHFYPIWEAA 100
    SLDEWLYNGG PYQLVVFHFL IGIFCYMGRQ WELSYRLGMR PWICVAYSAP 150
    VSAATAVFLI YPIGQGSFSD GMPLGISGTF NFMIVFQAEH NILMHPFHML 200
    GVAGVFGGSL FSAMHGSLVT SSLVRETTEV ESQNYGYKFG QEEETYNIVA 250
    AHGYFGRLIF QYASFNNSRS LHFFLGAWPV IGIWFTAMGV STMAFNLNGF 300
    NFNQSILDSQ GRVIGTWADV LNRANIGFEV MHERNAHNFP LDLASGEQAP 350
    VALTAPAVNG 360
    Length:360
    Mass (Da):39,721
    Last modified:April 1, 1990 - v1
    Checksum:i2FE4088498A416C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56000 Genomic DNA. Translation: CAA39472.1.
    X13547 Genomic DNA. Translation: CAA31899.1.
    BA000022 Genomic DNA. Translation: BAA18230.1.
    BA000022 Genomic DNA. Translation: BAA16586.1.
    PIRiS13112. F2YB16.
    RefSeqiNP_439906.1. NC_000911.1.
    NP_441550.1. NC_000911.1.
    YP_005649961.1. NC_017277.1.
    YP_005651608.1. NC_017277.1.
    YP_007449789.1. NC_020286.1.
    YP_007451432.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA16586; BAA16586; BAA16586.
    BAA18230; BAA18230; BAA18230.
    GeneIDi951890.
    953105.
    KEGGisyn:sll1867.
    syn:slr1311.
    syy:SYNGTS_0008.
    syy:SYNGTS_1655.
    syz:MYO_116700.
    syz:MYO_180.
    PATRICi23836870. VBISynSp132158_0010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56000 Genomic DNA. Translation: CAA39472.1 .
    X13547 Genomic DNA. Translation: CAA31899.1 .
    BA000022 Genomic DNA. Translation: BAA18230.1 .
    BA000022 Genomic DNA. Translation: BAA16586.1 .
    PIRi S13112. F2YB16.
    RefSeqi NP_439906.1. NC_000911.1.
    NP_441550.1. NC_000911.1.
    YP_005649961.1. NC_017277.1.
    YP_005651608.1. NC_017277.1.
    YP_007449789.1. NC_020286.1.
    YP_007451432.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P16033.
    SMRi P16033. Positions 10-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P16033. 5 interactions.
    STRINGi 1148.slr1311.

    Protein family/group databases

    TCDBi 3.E.2.2.2. the photosynthetic reaction center (prc) family.

    Proteomic databases

    PaxDbi P16033.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA16586 ; BAA16586 ; BAA16586 .
    BAA18230 ; BAA18230 ; BAA18230 .
    GeneIDi 951890.
    953105.
    KEGGi syn:sll1867.
    syn:slr1311.
    syy:SYNGTS_0008.
    syy:SYNGTS_1655.
    syz:MYO_116700.
    syz:MYO_180.
    PATRICi 23836870. VBISynSp132158_0010.

    Phylogenomic databases

    eggNOGi NOG04871.
    HOGENOMi HOG000246913.
    KOi K02703.
    OMAi CFTIAFI.
    OrthoDBi EOG6Q2SGP.
    PhylomeDBi P16033.

    Family and domain databases

    Gene3Di 1.20.85.10. 1 hit.
    HAMAPi MF_01379. PSII_PsbA_D1.
    InterProi IPR000484. Photo_RC_L/M.
    IPR005867. PSII_D1.
    [Graphical view ]
    Pfami PF00124. Photo_RC. 1 hit.
    [Graphical view ]
    PRINTSi PR00256. REACTNCENTRE.
    SUPFAMi SSF81483. SSF81483. 1 hit.
    TIGRFAMsi TIGR01151. psbA. 1 hit.
    PROSITEi PS00244. REACTION_CENTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a second psbA gene from the unicellular cyanobacterium Synechocystis 6803."
      Ravnikar P.D., Debus R., Sevrinck J., Saetaert P., McIntosh L.
      Nucleic Acids Res. 17:3991-3991(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence of the psbA3 gene from the cyanobacterium Synechocystis PCC 6803."
      Metz J., Nixon P., Diner B.
      Nucleic Acids Res. 18:6715-6715(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.
    4. "Mutations in the D1 subunit of photosystem II distinguish between quinone and herbicide binding sites."
      Ohad N., Hirschberg J.
      Plant Cell 4:273-282(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS FOR HERBICIDE RESISTANCE.
    5. "The D-E region of the D1 protein is involved in multiple quinone and herbicide interactions in photosystem II."
      Kless H., Oren-Shamir M., Malkin S., McIntosh L., Edelman M.
      Biochemistry 33:10501-10507(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS FOR HERBICIDE RESISTANCE.
    6. "Thylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis."
      Pursiheimo S., Rintamaeki E., Baena-Gonzalez E., Aro E.-M.
      FEBS Lett. 423:178-182(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, LACK OF PHOSPHORYLATION.
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiPSBA2_SYNY3
    AccessioniPrimary (citable) accession number: P16033
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Herbicides such as atrazine, BNT, diuron or ioxynil bind to Q(B) and block electron transport.
    Cyanobacteria usually contain more than 2 copies of the psbA gene.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3