Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Photosystem II protein D1 2

Gene

psbA2

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Photosystem II (PSII) is a light-driven water: plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.UniRule annotation

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

Note: The D1/D2 heterodimer binds P680, chlorophylls that are the primary electron donor of PSII, and subsequent electron acceptors. It shares a non-heme iron and each subunit binds pheophytin, quinone, additional chlorophylls, carotenoids and lipids. D1 provides most of the ligands for the Mn4-Ca-O5 cluster of the oxygen-evolving complex (OEC). There is also a Cl(-1) ion associated with D1 and D2, which is required for oxygen evolution. The PSII complex binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi118 – 1181Magnesium (chlorophyll-a ChlzD1 axial ligand); via tele nitrogenUniRule annotation
Binding sitei126 – 1261Pheophytin D1UniRule annotation
Sitei161 – 1611Tyrosine radical intermediateUniRule annotation
Metal bindingi170 – 1701Calcium-manganese-oxide [Ca-4Mn-5O]; calciumUniRule annotation
Metal bindingi170 – 1701Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation
Metal bindingi189 – 1891Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation
Sitei190 – 1901Stabilizes free radical intermediateUniRule annotation
Metal bindingi198 – 1981Magnesium (chlorophyll-a PD1 axial ligand); via tele nitrogenUniRule annotation
Metal bindingi215 – 2151Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation
Binding sitei215 – 2151Quinone (B)UniRule annotation
Metal bindingi272 – 2721Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation
Metal bindingi332 – 3321Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1; via tele nitrogenUniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 3UniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2UniRule annotation
Sitei344 – 3452Cleavage; by CtpAUniRule annotation1 Publication
Metal bindingi344 – 3441Calcium-manganese-oxide [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation
Metal bindingi344 – 3441Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2; via carboxylateUniRule annotation

GO - Molecular functioni

  1. chlorophyll binding Source: UniProtKB-HAMAP
  2. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
  3. iron ion binding Source: UniProtKB-HAMAP
  4. oxygen evolving activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. photosynthetic electron transport in photosystem II Source: InterPro
  2. protein-chromophore linkage Source: UniProtKB-KW
  3. response to herbicide Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Herbicide resistance, Photosynthesis, Transport

Keywords - Ligandi

Calcium, Chlorophyll, Chromophore, Iron, Magnesium, Manganese, Metal-binding

Protein family/group databases

TCDBi3.E.2.2.2. the photosynthetic reaction center (prc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II protein D1 2UniRule annotation (EC:1.10.3.9UniRule annotation)
Short name:
PSII D1 protein 2UniRule annotation
Alternative name(s):
Photosystem II Q(B) protein 2UniRule annotation
Gene namesi
Name:psbA2UniRule annotationCurated
Synonyms:psbA-2
Ordered Locus Names:slr1311
AND
Name:psbA3UniRule annotationCurated
Synonyms:psbA-3
Ordered Locus Names:sll1867
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cellular thylakoid membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4618HelicalUniRule annotationAdd
BLAST
Transmembranei118 – 13316HelicalUniRule annotationAdd
BLAST
Transmembranei142 – 15615HelicalUniRule annotationAdd
BLAST
Transmembranei197 – 21822HelicalUniRule annotationAdd
BLAST
Transmembranei274 – 28815HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane-derived thylakoid photosystem II Source: UniProtKB
  3. thylakoid membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi221 – 2222SS → LA: Strong herbicide resistance. 1 Publication
Mutagenesisi227 – 2282TT → AA: Herbicide resistance. 1 Publication
Mutagenesisi237 – 2371Y → F: Weak herbicide resistance. 1 Publication
Mutagenesisi238 – 2381K → V: Weak herbicide resistance. 1 Publication
Mutagenesisi255 – 2551F → W: Herbicide resistance. 1 Publication
Mutagenesisi257 – 2571R → V: Herbicide resistance. 1 Publication
Mutagenesisi264 – 2641S → A: Strong herbicide resistance. 1 Publication
Mutagenesisi271 – 2711L → V, M, A or S: Herbicide resistance. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Photosystem II protein D1 2UniRule annotationPRO_0000090493Add
BLAST
Propeptidei345 – 36016UniRule annotation1 PublicationPRO_0000316429Add
BLAST

Post-translational modificationi

C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and photosynthetic growth.UniRule annotation1 Publication
Tyr-161 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.UniRule annotation
C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and thus photosynthetic growth.UniRule annotation

Proteomic databases

PaxDbiP16033.

Interactioni

Subunit structurei

Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation

Protein-protein interaction databases

IntActiP16033. 5 interactions.
STRINGi1148.slr1311.

Structurei

3D structure databases

ProteinModelPortaliP16033.
SMRiP16033. Positions 10-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni264 – 2652Quinone (B)UniRule annotation

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG04871.
HOGENOMiHOG000246913.
InParanoidiP16033.
KOiK02703.
OMAiCFTIAFI.
OrthoDBiEOG6Q2SGP.
PhylomeDBiP16033.

Family and domain databases

Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTLQQRES ASLWEQFCQW VTSTNNRIYV GWFGTLMIPT LLTATTCFII
60 70 80 90 100
AFIAAPPVDI DGIREPVAGS LLYGNNIISG AVVPSSNAIG LHFYPIWEAA
110 120 130 140 150
SLDEWLYNGG PYQLVVFHFL IGIFCYMGRQ WELSYRLGMR PWICVAYSAP
160 170 180 190 200
VSAATAVFLI YPIGQGSFSD GMPLGISGTF NFMIVFQAEH NILMHPFHML
210 220 230 240 250
GVAGVFGGSL FSAMHGSLVT SSLVRETTEV ESQNYGYKFG QEEETYNIVA
260 270 280 290 300
AHGYFGRLIF QYASFNNSRS LHFFLGAWPV IGIWFTAMGV STMAFNLNGF
310 320 330 340 350
NFNQSILDSQ GRVIGTWADV LNRANIGFEV MHERNAHNFP LDLASGEQAP
360
VALTAPAVNG
Length:360
Mass (Da):39,721
Last modified:April 1, 1990 - v1
Checksum:i2FE4088498A416C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56000 Genomic DNA. Translation: CAA39472.1.
X13547 Genomic DNA. Translation: CAA31899.1.
BA000022 Genomic DNA. Translation: BAA18230.1.
BA000022 Genomic DNA. Translation: BAA16586.1.
PIRiS13112. F2YB16.
RefSeqiNP_439906.1. NC_000911.1.
NP_441550.1. NC_000911.1.
YP_005649961.1. NC_017277.1.
YP_005651608.1. NC_017277.1.
YP_007449789.1. NC_020286.1.
YP_007451432.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA16586; BAA16586; BAA16586.
BAA18230; BAA18230; BAA18230.
GeneIDi951890.
953105.
KEGGisyn:sll1867.
syn:slr1311.
PATRICi23836870. VBISynSp132158_0010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56000 Genomic DNA. Translation: CAA39472.1.
X13547 Genomic DNA. Translation: CAA31899.1.
BA000022 Genomic DNA. Translation: BAA18230.1.
BA000022 Genomic DNA. Translation: BAA16586.1.
PIRiS13112. F2YB16.
RefSeqiNP_439906.1. NC_000911.1.
NP_441550.1. NC_000911.1.
YP_005649961.1. NC_017277.1.
YP_005651608.1. NC_017277.1.
YP_007449789.1. NC_020286.1.
YP_007451432.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP16033.
SMRiP16033. Positions 10-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16033. 5 interactions.
STRINGi1148.slr1311.

Protein family/group databases

TCDBi3.E.2.2.2. the photosynthetic reaction center (prc) family.

Proteomic databases

PaxDbiP16033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16586; BAA16586; BAA16586.
BAA18230; BAA18230; BAA18230.
GeneIDi951890.
953105.
KEGGisyn:sll1867.
syn:slr1311.
PATRICi23836870. VBISynSp132158_0010.

Phylogenomic databases

eggNOGiNOG04871.
HOGENOMiHOG000246913.
InParanoidiP16033.
KOiK02703.
OMAiCFTIAFI.
OrthoDBiEOG6Q2SGP.
PhylomeDBiP16033.

Family and domain databases

Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a second psbA gene from the unicellular cyanobacterium Synechocystis 6803."
    Ravnikar P.D., Debus R., Sevrinck J., Saetaert P., McIntosh L.
    Nucleic Acids Res. 17:3991-3991(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the psbA3 gene from the cyanobacterium Synechocystis PCC 6803."
    Metz J., Nixon P., Diner B.
    Nucleic Acids Res. 18:6715-6715(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  4. "Mutations in the D1 subunit of photosystem II distinguish between quinone and herbicide binding sites."
    Ohad N., Hirschberg J.
    Plant Cell 4:273-282(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PHE-255; SER-264 AND LEU-271.
  5. "The D-E region of the D1 protein is involved in multiple quinone and herbicide interactions in photosystem II."
    Kless H., Oren-Shamir M., Malkin S., McIntosh L., Edelman M.
    Biochemistry 33:10501-10507(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 221-SER-SER-222; 227-THR-THR-228; TYR-237; LYS-238 AND ARG-257.
  6. "Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease. Analysis of a spontaneous photosystem II-deficient mutant strain of the cyanobacterium Synechocystis sp. PCC 6803."
    Shestakov S.V., Anbudurai P.R., Stanbekova G.E., Gadzhiev A., Lind L.K., Pakrasi H.B.
    J. Biol. Chem. 269:19354-19359(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE CLEAVAGE.
    Strain: ATCC 27184 / PCC 6803 / N-1.
  7. "Thylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis."
    Pursiheimo S., Rintamaeki E., Baena-Gonzalez E., Aro E.-M.
    FEBS Lett. 423:178-182(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, LACK OF PHOSPHORYLATION.
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiPSBA2_SYNY3
AccessioniPrimary (citable) accession number: P16033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 4, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Cyanobacteria usually contain more than 2 copies of the psbA gene.UniRule annotation
2 of the reaction center chlorophylls (ChlD1 and ChlD2) are entirely coordinated by water.UniRule annotation
Herbicides such as atrazine, BNT, diuron or ioxynil bind in the Q(B) binding site and block subsequent electron transfer.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.