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P16033 (PSBA2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Photosystem Q(B) protein 2

EC=1.10.3.9
Alternative name(s):
32 kDa thylakoid membrane protein 2
Photosystem II protein D1 2
Gene names
Name:psbA2
Synonyms:psbA-2
Ordered Locus Names:slr1311
AND
Name:psbA3
Synonyms:psbA-3
Ordered Locus Names:sll1867
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of the two reaction center proteins of photosystem II By similarity. HAMAP-Rule MF_01379

Catalytic activity

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol. HAMAP-Rule MF_01379

Cofactor

The PsbA/B heterodimer binds P680, the primary electron donor of PSII. It shares a non-heme iron and each subunit binds additional chlorophylls and pheophytin. PsbA provides most of the ligands for the Mn-cluster of the oxygen-evolving complex By similarity.

Subunit structure

The PsbA/B heterodimer binds the P680 chlorophylls and subsequent electron acceptors. PSII consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. PSII forms dimeric complexes By similarity.

Subcellular location

Cellular thylakoid membrane; Multi-pass membrane protein Probable Ref.6.

Miscellaneous

Herbicides such as atrazine, BNT, diuron or ioxynil bind to Q(B) and block electron transport.

Cyanobacteria usually contain more than 2 copies of the psbA gene.

Sequence similarities

Belongs to the reaction center PufL/M/PsbA/D family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Photosystem Q(B) protein 2 HAMAP-Rule MF_01379
PRO_0000090493
Propeptide345 – 36016 Potential
PRO_0000316429

Regions

Transmembrane36 – 5621Helical; Potential
Transmembrane109 – 12921Helical; Potential
Transmembrane141 – 16424Helical; Potential
Transmembrane192 – 21827Helical; Potential
Transmembrane269 – 28921Helical; Potential

Sites

Metal binding1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calcium By similarity
Metal binding1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1 By similarity
Metal binding1891Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2 By similarity
Metal binding2151Iron; shared with heterodimeric partner By similarity
Metal binding2721Iron; shared with heterodimeric partner By similarity
Metal binding3321Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2; via tele nitrogen By similarity
Metal binding3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1 By similarity
Metal binding3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 3 By similarity
Metal binding3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2 By similarity
Metal binding3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4 By similarity
Metal binding3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calcium; via carboxylate By similarity
Metal binding3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4; via carboxylate By similarity
Site344 – 3452Cleavage; by CtpA By similarity

Experimental info

Mutagenesis221 – 2222SS → LA: Strong herbicide resistance.
Mutagenesis227 – 2282TT → AA: Herbicide resistance.
Mutagenesis2371Y → F: Weak herbicide resistance.
Mutagenesis2381K → V: Weak herbicide resistance.
Mutagenesis2551F → W: Herbicide resistance.
Mutagenesis2571R → V: Herbicide resistance.
Mutagenesis2641S → A: Strong herbicide resistance.
Mutagenesis2711L → V, M, A or S: Herbicide resistance.

Sequences

Sequence LengthMass (Da)Tools
P16033 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 2FE4088498A416C2

FASTA36039,721
        10         20         30         40         50         60 
MTTTLQQRES ASLWEQFCQW VTSTNNRIYV GWFGTLMIPT LLTATTCFII AFIAAPPVDI 

        70         80         90        100        110        120 
DGIREPVAGS LLYGNNIISG AVVPSSNAIG LHFYPIWEAA SLDEWLYNGG PYQLVVFHFL 

       130        140        150        160        170        180 
IGIFCYMGRQ WELSYRLGMR PWICVAYSAP VSAATAVFLI YPIGQGSFSD GMPLGISGTF 

       190        200        210        220        230        240 
NFMIVFQAEH NILMHPFHML GVAGVFGGSL FSAMHGSLVT SSLVRETTEV ESQNYGYKFG 

       250        260        270        280        290        300 
QEEETYNIVA AHGYFGRLIF QYASFNNSRS LHFFLGAWPV IGIWFTAMGV STMAFNLNGF 

       310        320        330        340        350        360 
NFNQSILDSQ GRVIGTWADV LNRANIGFEV MHERNAHNFP LDLASGEQAP VALTAPAVNG 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a second psbA gene from the unicellular cyanobacterium Synechocystis 6803."
Ravnikar P.D., Debus R., Sevrinck J., Saetaert P., McIntosh L.
Nucleic Acids Res. 17:3991-3991(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the psbA3 gene from the cyanobacterium Synechocystis PCC 6803."
Metz J., Nixon P., Diner B.
Nucleic Acids Res. 18:6715-6715(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
[4]"Mutations in the D1 subunit of photosystem II distinguish between quinone and herbicide binding sites."
Ohad N., Hirschberg J.
Plant Cell 4:273-282(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS FOR HERBICIDE RESISTANCE.
[5]"The D-E region of the D1 protein is involved in multiple quinone and herbicide interactions in photosystem II."
Kless H., Oren-Shamir M., Malkin S., McIntosh L., Edelman M.
Biochemistry 33:10501-10507(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS FOR HERBICIDE RESISTANCE.
[6]"Thylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis."
Pursiheimo S., Rintamaeki E., Baena-Gonzalez E., Aro E.-M.
FEBS Lett. 423:178-182(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, LACK OF PHOSPHORYLATION.
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56000 Genomic DNA. Translation: CAA39472.1.
X13547 Genomic DNA. Translation: CAA31899.1.
BA000022 Genomic DNA. Translation: BAA18230.1.
BA000022 Genomic DNA. Translation: BAA16586.1.
PIRF2YB16. S13112.
RefSeqNP_439906.1. NC_000911.1.
NP_441550.1. NC_000911.1.
YP_005649961.1. NC_017277.1.
YP_005651608.1. NC_017277.1.
YP_007449789.1. NC_020286.1.
YP_007451432.1. NC_020286.1.

3D structure databases

ProteinModelPortalP16033.
SMRP16033. Positions 10-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP16033. 5 interactions.
STRING1148.slr1311.

Protein family/group databases

TCDB3.E.2.2.2. the photosynthetic reaction center (prc) family.

Proteomic databases

PaxDbP16033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA16586; BAA16586; BAA16586.
BAA18230; BAA18230; BAA18230.
GeneID12254260.
12255303.
14615430.
14617092.
951890.
953105.
KEGGsyn:sll1867.
syn:slr1311.
syy:SYNGTS_0008.
syy:SYNGTS_1655.
syz:MYO_116700.
syz:MYO_180.
PATRIC23836870. VBISynSp132158_0010.

Phylogenomic databases

eggNOGNOG04871.
HOGENOMHOG000246913.
KOK02703.
OMACFTIAFI.
OrthoDBEOG6Q2SGP.
PhylomeDBP16033.
ProtClustDBCLSK892431.

Family and domain databases

Gene3D1.20.85.10. 1 hit.
HAMAPMF_01379. PSII_PsbA_D1.
InterProIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSPR00256. REACTNCENTRE.
SUPFAMSSF81483. SSF81483. 1 hit.
TIGRFAMsTIGR01151. psbA. 1 hit.
PROSITEPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSBA2_SYNY3
AccessionPrimary (citable) accession number: P16033
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families