Reviewed, UniProtKB/Swiss-Prot P16020 (IPNS_FLASS)

Names and origin

Protein names

Recommended name:
    Isopenicillin N synthetase
    EC=1.21.3.1
Alternative name(s):
    Isopenicillin N synthase
      Short name=IPNS

Gene names

Name:

pcbC

Organism

Flavobacterium sp. (strain SC 12,154)

Taxonomic identifier

241 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteria › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Isopenicillin N synthetase		PRO_0000219500
Sites
	Metal binding	209	1	Iron By similarity
	Metal binding	211	1	Iron By similarity
	Metal binding	265	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16020-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 256E274316395837	FASTA	326	36,465
	10 20 30 40 50 60 MNRHADVPVI DISGLSGNDM DVKKDIAARI DRACRGSGFF YAANHGVDLA ALQKFTTDWH 70 80 90 100 110 120 MAMSAEEKWE LAIRAYNPAN PRNRNGYYMA VEGKKANESF CYLNPSFDAD HATIKAGLPS 130 140 150 160 170 180 HEVNIWPDEA RHPGMRRFYE AYFSDVFDVA AVILRGFAIA LGREESFFER HFSMDDTLSA 190 200 210 220 230 240 VSLIRYPFLE NYPPLKLGPD GEKLSFEHHQ DVSLITVLYQ TAIPNLQVET AEGYLDIPVS 250 260 270 280 290 300 DEHFLVNCGT YMAHITNGYY PAPVHRVKYI NAERLSIPFF ANLSHASAID PFAPPPYAPP 310 320 GGNPTVSYGD YLQHGLLDLI RANGQT

References

[1]

"Nucleotide sequence of the isopenicillin N synthase gene (pcbC) of the Gram-negative Flavobacterium sp. SC 12,154." Shiffman D., Cohen G., Aharonowitz Y., von Dohren H., Kleinkauf H., Mevarech M. Nucleic Acids Res. 18:660-660(1990) [PubMed: 2308852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17355 Genomic DNA. Translation: CAA35233.1.
3D structure databases
HSSP	HSSP built from PDB template 1ODM based on UniProtKB P05326.
ModBase	Search...
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_FLASS

Accession

Primary (citable) accession number: P16020

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)