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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 208GTPUniRule annotation
Nucleotide bindingi90 – 945GTPUniRule annotation
Nucleotide bindingi145 – 1484GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Synonyms:eef1a
Ordered Locus Names:rrnAC2406
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
Proteomesi
  • UP000001169 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 421420Elongation factor 1-alphaPRO_0000090977Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272569.rrnAC2406.

Structurei

3D structure databases

ProteinModelPortaliP16018.
SMRiP16018. Positions 5-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 220217tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 208G1By similarity
Regioni69 – 735G2By similarity
Regioni90 – 934G3By similarity
Regioni145 – 1484G4By similarity
Regioni184 – 1863G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiVNYDQKR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDEQHQNLA IIGHVDHGKS TLVGRLLYET GSVPEHVIEQ HKEEAEEKGK
60 70 80 90 100
GGFEFAYVMD NLAEERERGV TIDIAHQEFS TDTYDFTIVD CPGHRDFVKN
110 120 130 140 150
MITGASQADN AVLVVAADDG VQPQTQEHVF LARTLGIGEL IVAVNKMDLV
160 170 180 190 200
DYGESEYKQV VEEVKDLLTQ VRFDSENAKF IPVSAFEGDN IAEESEHTGW
210 220 230 240 250
YDGEILLEAL NELPAPEPPT DAPLRLPIQD VYTISGIGTV PVGRVETGIL
260 270 280 290 300
NTGDNVSFQP SDVSGEVKTV EMHHEEVPKA EPGDNVGFNV RGVGKDDIRR
310 320 330 340 350
GDVCGPADDP PSVAETFQAQ IVVMQHPSVI TEGYTPVFHA HTAQVACTVE
360 370 380 390 400
SIDKKIDPSS GEVAEENPDF IQNGDAAVVT VRPQKPLSIE PSSEIPELGS
410 420
FAIRDMGQTI AAGKVLGVNE R
Length:421
Mass (Da):45,731
Last modified:January 23, 2007 - v2
Checksum:iCBA81BA0722000A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16677 Genomic DNA. Translation: CAA34665.1.
AY596297 Genomic DNA. Translation: AAV47220.1.
PIRiS15761. EFHST.
RefSeqiWP_007189404.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV47220; AAV47220; rrnAC2406.
GeneIDi3129349.
KEGGihma:rrnAC2406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16677 Genomic DNA. Translation: CAA34665.1.
AY596297 Genomic DNA. Translation: AAV47220.1.
PIRiS15761. EFHST.
RefSeqiWP_007189404.1. NC_006396.1.

3D structure databases

ProteinModelPortaliP16018.
SMRiP16018. Positions 5-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272569.rrnAC2406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV47220; AAV47220; rrnAC2406.
GeneIDi3129349.
KEGGihma:rrnAC2406.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiVNYDQKR.

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2171-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A_HALMA
AccessioniPrimary (citable) accession number: P16018
Secondary accession number(s): Q5UZT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.