Reviewed, UniProtKB/Swiss-Prot P16016 (CAHC_SPIOL)

Names and origin

Protein names	Recommended name:     Carbonic anhydrase, chloroplastic     EC=4.2.1.1 Alternative name(s):     Carbonate dehydratase
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Subunit structure	Homohexamer.
Subcellular location	Plastid › chloroplast stroma.
Sequence similarities	Belongs to the beta-class carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Zinc
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbon utilization Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 98	98	Chloroplast Ref.2 Ref.3
	Chain	99 – 319	221	Carbonic anhydrase, chloroplastic		PRO_0000004271
Experimental info
	Sequence conflict	99	1	E → G AA sequence Ref.3
	Sequence conflict	222	1	F → S in AAA34026. Ref.2
	Sequence conflict	311	1	Y → F in AAA34026. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P16016-1 [UniParc]. Last modified August 1, 1990. Version 2. Checksum: 2229B19F02423EEF	FASTA	319	34,570
	10 20 30 40 50 60 MSTINGCLTS ISPSRTQLKN TSTLRPTFIA NSRVNPSSSV PPSLIRNQPV FAAPAPIITP 70 80 90 100 110 120 TLKEDMAYEE AIAALKKLLS EKGELENEAA SKVAQITSEL ADGGTPSASY PVQRIKEGFI 130 140 150 160 170 180 KFKKEKYEKN PALYGELSKG QAPKFMVFAC SDSRVCPSHV LDFQPGEAFM VRNIANMVPV 190 200 210 220 230 240 FDKDKYAGVG AAIEYAVLHL KVENIVVIGH SACGGIKGLM SFPDAGPTTT DFIEDWVKIC 250 260 270 280 290 300 LPAKHKVLAE HGNATFAEQC THCEKEAVNV SLGNLLTYPF VRDGLVKKTL ALQGGYYDFV 310 NGSFELWGLE YGLSPSQSV

References

[1]	"Spinach carbonic anhydrase primary structure deduced from the sequence of a cDNA clone." Fawcett T.W., Browse J.A., Volokita M., Bartlett S.G. J. Biol. Chem. 265:5414-5417(1990) [PubMed: 2108138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Hybrid 424.
[2]	"Spinach chloroplastic carbonic anhydrase: nucleotide sequence analysis of cDNA." Burnell J.N., Gibbs M.J., Mason J.G. Plant Physiol. 92:37-40(1990) [PubMed: 16667262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 66-319, PROTEIN SEQUENCE OF 99-118.
[3]	"Origins and molecular evolution of the carbonic anhydrase isozymes." Hewett-Emmett D., Hopkins P.J., Tashian R.E., Czelusniak J. Ann. N. Y. Acad. Sci. 429:338-358(1984) [PubMed: 6430173] [Abstract] Cited for: PROTEIN SEQUENCE OF 99-118.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05403 mRNA. Translation: AAA34027.1. M27295 mRNA. Translation: AAA34026.1.
PIR	A35163.
3D structure databases
HSSP	HSSP built from PDB template 1EKJ based on UniProtKB P17067.
SMR	P16016. Positions 113-319.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.1.1. 286.
Family and domain databases
InterPro	IPR001765. Carbonic_anhydrase. IPR015892. Carbonic_anhydrase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1050.10. CO_anhd_prok_pln. 1 hit.
PANTHER	PTHR11002. Carbonic_anhydrase. 1 hit.
Pfam	PF00484. Pro_CA. 1 hit. [Graphical view]
PROSITE	PS00704. PROK_CO2_ANHYDRASE_1. 1 hit. PS00705. PROK_CO2_ANHYDRASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAHC_SPIOL

Accession

Primary (citable) accession number: P16016

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)